Association between High Diffusion-Weighted Imaging-Derived Functional Tumor Burden of Peritoneal Carcinomatosis and Overall Survival in Patients with Advanced Ovarian Carcinoma

An He,Perucho Jose AU,Chiu Keith WH,Hui Edward S,Chu Mandy MY,Ngu Siew Fei,Ngan Hextan YS,Lee Elaine YP 대한영상의학회 2022 Korean Journal of Radiology Vol.23 No.5

Objective: To investigate the association between functional tumor burden of peritoneal carcinomatosis (PC) derived from diffusion-weighted imaging (DWI) and overall survival in patients with advanced ovarian carcinoma (OC). Materials and Methods: This prospective study was approved by the local research ethics committee, and informed consent was obtained. Fifty patients (mean age ± standard deviation, 57 ± 12 years) with stage III–IV OC scheduled for primary or interval debulking surgery (IDS) were recruited between June 2016 and December 2021. DWI (b values: 0, 400, and 800 s/mm2) was acquired with a 16-channel phased-array torso coil. The functional PC burden on DWI was derived based on K-means clustering to discard fat, air, and normal tissue. A score similar to the surgical peritoneal cancer index was assigned to each abdominopelvic region, with additional scores assigned to the involvement of critical sites, denoted as the functional peritoneal cancer index (fPCI). The apparent diffusion coefficient (ADC) of the largest lesion was calculated. Patients were dichotomized by immediate surgical outcome into high- and low-risk groups (with and without residual disease, respectively) with subsequent survival analysis using the Kaplan-Meier curve and log-rank test. Multivariable Cox proportional hazards regression was used to evaluate the association between DWI-derived results and overall survival. Results: Fifteen (30.0%) patients underwent primary debulking surgery, and 35 (70.0%) patients received neoadjuvant chemotherapy followed by IDS. Complete tumor debulking was achieved in 32 patients. Patients with residual disease after debulking surgery had reduced overall survival (p = 0.043). The fPCI/ADC was negatively associated with overall survival when accounted for clinicopathological information with a hazard ratio of 1.254 for high fPCI/ADC (95% confidence interval, 1.007–1.560; p = 0.043). Conclusion: A high DWI-derived functional tumor burden was associated with decreased overall survival in patients with advanced OC.

The Design As Culture

He Song Fei (사)한국디지털디자인협의회 2006 (사)한국디지털디자인협의회 conference Vol.2006 No.2

SAXS investigations of the fractal character of additive silica xerogels

Fei He,Xiaodong He,Mingwei Li,Sumei Zhang 한양대학교 세라믹연구소 2008 Journal of Ceramic Processing Research Vol.9 No.4

Two-step acid-base catalyzed silica xerogels with different amounts of additives, including TiO2 powder, silica xerogel powders and short glass fibers, were prepared through a sol-gel route and ambient pressure drying. The fractal dimensions of these additive silica xerogels were investigated by the means of small-angle X-ray scattering (SAXS). Specific surface area and the average chord length were calculated by the Debye method. The changes of the porous structure were compared between the pure silica xerogels and the impure ones. The results show that additive silica xerogels do not possess a surface fractal character. The mass fractal range of silica xerogels decreases and colloid particle size increases with an increase of additives. The adding of excessive silica xerogel powders results in agglomeration of particles which goes beyond the metrical limit of SAXS. The mass fractal dimension and fractal range decrease with the addition of additional short fibers. The shrinkage was restrained in the course of drying by adding short fibers Two-step acid-base catalyzed silica xerogels with different amounts of additives, including TiO2 powder, silica xerogel powders and short glass fibers, were prepared through a sol-gel route and ambient pressure drying. The fractal dimensions of these additive silica xerogels were investigated by the means of small-angle X-ray scattering (SAXS). Specific surface area and the average chord length were calculated by the Debye method. The changes of the porous structure were compared between the pure silica xerogels and the impure ones. The results show that additive silica xerogels do not possess a surface fractal character. The mass fractal range of silica xerogels decreases and colloid particle size increases with an increase of additives. The adding of excessive silica xerogel powders results in agglomeration of particles which goes beyond the metrical limit of SAXS. The mass fractal dimension and fractal range decrease with the addition of additional short fibers. The shrinkage was restrained in the course of drying by adding short fibers

The Design As Culture

He Song Fei 한국디자인지식학회 2006 한국디자인지식학회 Conference Vol.2006 No.8

Research on the Sustainable Development of Chinese Regional Food Culture Integrating Service Design Thinking

He, Song Fei(하송비),Liang, Yue Hui(양월휘),Chung, Mi Sun(정미선) 한국전시산업융합연구원 2021 한국과학예술융합학회 Vol.39 No.2

This study begins with the phenomenon that cultural conflict may occur between people with different social backgrounds, lifestyles and values in the context of social and economic globalization. Cultural conflict is one of the preconditions to promote cultural identity, and cultural identity is also the basis for the sustainable development of regional culture. With the rapid development of society and the substantial improvement of material conditions, food and clothing is no longer a social problem in China. People begin to pay attention to their own internal needs and have more diversified demands on the connotative value and presentation of food. As a symbol of regional culture, food can reflect the internal culture of the region and the social and cultural relationship between the region and other regions. It is an important carrier of the sustainable development of regional culture. In the face of the increasing demand at all levels, tangible single products can no longer meet the needs of users, thus promoting the market economy from product oriented to service-oriented competition. The purpose of this study is to deconstruct and reconstruct a variety of possibilities between regional food, regional culture and people, give play to the role of service design thinking in this process, and explore the value and significance of service design thinking in the process of sustainable development of regional food culture. Therefore, this paper attempts to use service design thinking to participate in the research process of the sustainable development of Chinese regional culture, and its methods and tools to participate in the process of regional culture characteristic food is not only the food remanufacturing and so on. The key is to study and analyze the relationship between the cultural attributes and lifestyle of regional food, create design value by means of symbols, vision, products, space and service process, and enhance the cultural significance and commercial attributes of food with the intervention of multi stakeholders. The research results and contents are as follows: firstly, as the determination and expression of regional food cultural connotation information, service design is not only the presentation of food material attribute information, but also the fundamental driving force to promote the development of intangible values (information, memory, interaction, etc.) such as its cultural attribute. Secondly, the food system involved in service design pays attention to the experience perception of users, promotes collaborative innovation activities of regional food stakeholders, maximizes the value of regional cultural information obtained by stakeholders in the service system, cooperatively creates possible solutions and improves the service quality of the food system. Thirdly, service design improves the user experience of regional food culture, explores the interactive relationship between people and time, region, culture, food and other contacts, and uses symbols and other means of information expression to play the role of people in promoting and inheriting the sustainable development of culture in the regional food system. Based on the research results, this paper looks forward to the ability and creativity of service design to solve the problem of sustainable development of food culture with regional characteristics, promote the advanced innovation of food culture with regional characteristics, and transmit and develop more diversified cultural values.

Inferring Single Nucleotide Polymorphisms in MicroRNA Binding Sites of Lung Cancer-related Inflammatory Genes

He, Fei,Zheng, Ling-Ling,Luo, Wen-Ting,Yang, Rong,Xu, Xiao-Qin,Cai, Lin Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.8

Single nucleotide polymorphisms located at microRNA (miRNA)-binding sites are likely to affect the expression of miRNA targets and may contribute to the susceptibility of humans to common diseases. Here 335 candidate lung cancer-related inflammatory genes were selected according to the existing literature and database. We identified putative miRNA-binding sites of 149 genes by specialised algorithms and screened SNPs in the 3'UTRs of these genes. By calculating binding free energy, we sorted 269 SNPs on the basis of the possibility of prediction. The proposed approach could help to easy the identification of functionally relevant SNPs and minimize the workflow and the costs.

Promoting Preschoolers' Contact Ability and Sense of Security through Aesthetic Education

Wang He Fei 국제문화기술진흥원 2023 International Journal of Advanced Culture Technolo Vol.11 No.1

Nowadays, more and more countries are paying attention to basic teaching, so it can be found that they are carrying out a new round of basic teaching reform. The fundamental purpose is to adapt to the economic, cultural and technological development of the 21st century, so that their nationals will not be eliminated in the competition of the world environment, the spiritual civilization and material life of the nationals can be further improved, and they can have an advantage in the competition and catch up with the advanced level of the world. The cultivation of preschoolers' mental health determines the future development of a person. Through the long-term practice of art teaching at the Longyuan Branch of the District Third Kindergarten of Guangxi, This paper summarizes some effective teaching methods to cultivate children's connection ability and sense of security through aesthetic education.Firstly, preschoolers' contact ability was studied, and the aspects such as the definition of contact ability and the reasons for the formation of contact ability were explored. Secondly, the development of preschoolers' sense of security, what to avoid in order to develop a good sense of security and the effects of security on children were investigated. Finally, several specific teaching cases were written based on these theories. Through these cases, aesthetic education was utilized to promote preschoolers' contact ability and develop a sense of security. The study mainly draws the following conclusions that if children want to have a successful career, a happy family in the future, it is crucial to have the ability of connect and security in childhood. Aesthetic education can promote the formation of children's ability of connection and security.Finally, I write specific teaching cases based on the above theories and practice, through which these cases use aesthetic education to promote the formation of children's contact ability and sense of security.

A Secure Communication Scheme without Trusted RSU Setting for VANET

하비(He Fei),이쿤(Li Kun),김범한(Bum Han Kim),이동훈(Dong Hoon Lee) 한국정보보호학회 2010 정보보호학회논문지 Vol.20 No.4

근접한 차량으로부터 수신한 브로드캐스트 메시지는 운전자와 승객의 생명과 직간접적으로 연관이 있는 메시지를 포함하고 있기 때문에 VANET에서 보안 통신은 주요 과제 중 하나가 되어 왔다. 이 때문에 보안 통신에 대한 다양한 기법들이 제안되어 왔다. 하지만, 그 기법들의 대부분은, 직접적으로 관리되지 않는 즉 방치된, RSU를 신뢰해야 한다는 가정에 기반을 두고 있다. 본 논문은 RSU를 신뢰할 필요가 없는 보안 통신 기법을 제안하며 사용자의 익명성을 위태롭게 하는 것 없이 서버에 지워지는 부담을 평균적으로 나누기 위하여 그룹핑 기술을 적용하였다. 게다가 본 논문에서는 상대적으로 낮은 하드웨어 성능을 요구하는 보안 요구 사항을 만족시키기 위하여 완전 집합(Complete set) 을 설계하였다. 마지막으로 본 논문은 보안 요구사항, 통신 오버헤드, 저장 용량, 그리고 네트워크 성능 측면에서 제안한 기법을 평가하였다. Secure communication has been one of the main challenges in vehicular ad hoc networks(VANET) since broadcast messages from nearby vehicles contain life-critical information for drivers and passengers. So far various secure communication schemes have been proposed to secure the communication in VANET, and they satisfy most security requirements. However most of them need to put trust on roadside units(RSUs), which are usually deployed in unattended area and vulnerable to compromise. In this paper, we propose a secure communication scheme, which does not need to put trust on RSUs. And we adopt a grouping technique to averagely divide the huge burden in the server without jeopardizing the anonymity of users. Moreover we design a complete set of protocols to satisfy common security requirements with a relatively lower hardware requirement. At last, we evaluate the scheme with respect to security requirements, communication overhead, storage overhead and network performance.

MiR-30c facilitates natural killer cell cytotoxicity to lung cancer through targeting GALNT7

Gao Fei,Han Jianjun,Jia Li,He Jun,Wang Yun,Chen Mi,Liu Xiaojun,He Xia 한국유전학회 2023 Genes & Genomics Vol.45 No.2

Background MicroRNAs (miRNAs) have been reported to play important roles in regulating natural killer (NK) cell cytotoxicity to cancer cells. Objective This study aimed to investigate the effects and potential mechanism of miR-30c in regulating NK cell cytotoxicity to lung cancer cells. Methods Primary NK cells were derived from the peripheral blood of lung cancer and normal participants. Exosomes were isolated and validated via transmission electron microscopy and nanoparticle tracking analysis. The levels of miR-30c, polypeptide N-acetylgalactosaminyltransferase 7 (GALNT7) and proteins in PI3K/AKT pathway were determined using quantitative real-time polymerase chain reaction or western blot. Tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ) levels and the cytotoxicity of effector NK cells to target lung cancer cells were measured via enzyme linked immunosorbent assay, cell apoptosis or xenograft experiments. The relationship between miR-30c and GALNT7 was analyzed by luciferase activity, RNA pull-down and RNA immunoprecipitation assays. And a xenograft mice model was established to verify the effect of miR-30c in regulating NK cell cytotoxicity to lung cancer cells in vivo. Results NK cell-derived exosomes carrying miR-30c, and miR-30c level was significantly downregulated in primary NK cells of lung cancer patients. MiR-30c overexpression promoted TNF-α and IFN-γ secretion and enhanced the cytotoxicity of interleukin 2 (IL-2)-treated NK cells to lung cancer cells, while knockdown of miR-30c played an opposite effect in regulating the cytotoxicity of NK cells to lung cancer cells. GALNT7 was a target of miR-30c and was negatively regulated by miR-30c. Besides, miR-30c targeted GALNT7 to exert its function in regulating NK cell cytotoxicity. Furthermore, GALNT7 prompted the activation of PI3K/AKT pathway in NK cells. Additionally, miR-30c overexpression enhanced NK cell cytotoxicity to lung cancer cells and inhibited tumor growth in vivo. Conclusion miR-30c enhanced NK cell cytotoxicity to lung cancer cells via decreasing GALNT7 and inactivating the PI3K/AKT pathway, suggesting that regulating miR-30c expression maybe a promising approach for enhancing NK cell-based antitumor therapies.

Identification of novel rheumatoid arthritis-associated MiRNA-204-5p from plasma exosomes

Wu Long-Fei,Zhang Qin,Mo Xing-Bo,Lin Jun,Wu Yang-Lin,Lu Xin,He Pei,Wu Jian,Guo Yu-Fan,Wang Ming-Jun,Ren Wen-Yan,Deng Hong-Wen,Lei Shu-Feng,Deng Fei-Yan 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

Rheumatoid arthritis (RA) is an autoimmune disease characterized by infiltration of immune cells in the synovium. However, the crosstalk of immune cells and synovial fibroblasts is still largely unknown. Here, global miRNA screening in plasma exosomes was carried out with a custom microarray (RA patients vs. healthy controls = 9:9). A total of 14 exosomal miRNAs were abnormally expressed in the RA patients. Then, downregulated expression of exosomal miR-204-5p was confirmed in both the replication (RA patients vs. healthy controls = 30:30) and validation groups (RA patients vs. healthy controls = 56:60). Similar to the findings obtained in humans, a decreased abundance of exosomal miR-204-5p was observed in mice with collagen-induced arthritis (CIA). Furthermore, Spearman correlation analysis indicated that plasma exosomal miR-204-5p expression was inversely correlated with disease parameters of RA patients, such as rheumatoid factor, erythrocyte sedimentation rate, and C-reactive protein. In vitro, our data showed that human T lymphocytes released exosomes containing large amounts of miR-204-5p, which can be transferred into synovial fibroblasts, inhibiting cell proliferation. Overexpression of miR-204-5p in synovial fibroblasts suppressed synovial fibroblast activation by targeting genes related to cell proliferation and invasion. In vivo assays found that administration of lentiviruses expressing miR-204-5p markedly alleviated the disease progression of the mice with CIA. Collectively, this study identified a novel RA-associated plasma exosomal miRNA-204-5p that mediates the communication between immune cells and synovial fibroblasts and can be used as a potential biomarker for RA diagnosis and treatment.