Turf Seedling Height and Quality in Paclobutrazol-treated Seeds of Lolium perenne L. ‘Barbal’ Sown in the Soil Mixed with Zeolite

Mahsa Shahrokhi,Hassan Salehi,Saeid Eshghi,Gholamreza Abdi 한국원예학회 2008 Horticulture, Environment, and Biotechnology Vol.49 No.6

To determine the effect of paclobutrazol on seedling height and visual quality, seeds of Lolium perenne L. ‘Barbal’ were soaked with 15, 30 and 45 ㎎?L<SUP>-1</SUP> paclobutrazol for 24 h on shaker during imbibitions stage of seed germination. Treated seeds were potted in the soil mixtures containing 5, 10, 15 and 30 percent zeolite with gardening soil and sand. 30 ㎎?L<SUP>-1</SUP> paclobutrazol followed by 15% zeolite resulted in the lowest seedling height. After the first mowing, lower heights were obtained in 30 ㎎?L<SUP>-1</SUP> paclobutrazol with 30% zeolite and in 45 ㎎?L<SUP>-1</SUP> paclobutrazol without zeolite. Paclobutrazol and zeolite combined at the highest concentrations resulted in the lowest height and clipping dry weight at second mowing. Treatment of 5 % zeolite had lower color quality but paclobutrazol at concentration of 15 ㎎?L<SUP>-1</SUP> produced the highest turf quality. It can be concluded that using zeolite in the media can lower the height of seedlings previously treated with paclobutrazol.

Effect of Natural Zeolite and Paclobutrazol on Reducing Salt Stress in Kentucky Bluegrass (Poa pratensis L.)

Gholamreza Abdi,Hassan Salehi,Saeid Eshghi 한국원예학회 2010 Horticulture, Environment, and Biotechnology Vol.51 No.3

A greenhouse experiment was conducted to study the effect of zeolite and drench application of paclobutrazol (PBZ) on the salt stress response. Kentucky bluegrass (Poa pratensis L.) turfs were grown in boxes filled with sand (100%), sand + 15% zeolite, sand + 30 ㎎ PBZ, and sand + 15% zeolite + 30 ㎎ PBZ, and were irrigated with 0.24 (control irrigation), 3.4 and 6.4 dS · m?¹ saline water daily for 6 months in greenhouse condition. Saline water reduced TQ, TFW, TDW, CY, PR, transpiration, SC, ME, and WUE, compared to control condition, Paclobutrazol and salinity treatments reduced growth and development, progressively produced plant that were too small especially at the highest saline concentration. Amendment of sand with zeolite increased TQ, TFW, TDW, CFW, CDW, PR, transpiration, SC, ME, and WUE during both second and third months of the experiment at both salinity levels. The beneficial effects of zeolite on turf quality and other parameters diminished 3 months after treatment. Amendment of sand with zeolite reduced leaching of Na and K but increased leaching of Ca?? and Mg??. Using zeolite in medium increased SAR value compared to control. Results indicated that amending with zeolite may buffer soil solution Na+ concentration in short-term. In the long-term, however, a substantial amount of Na? may be retained concurrent with Ca?? and Mg?? exchange, thereby increasing sodicity and salinity problems. Furthermore, results indicated that PBZ can reduce salinity effect via reducing the availability of salt ions in the medium.

Promotion of excisional wound repair by a menstrual blood-derived stem cell-seeded decellularized human amniotic membrane

Saeed Farzamfar,Majid Salehi,Arian Ehterami,Mahdi Naseri-Nosar,Ahmad Vaez,Amir-Hassan Zarnani,Hamed Sahrapeyma,Mohammad Reza Shokri,Mehdi Aleahmad 대한의용생체공학회 2018 Biomedical Engineering Letters (BMEL) Vol.8 No.4

This is the first study demonstrating the efficacy of menstrual blood-derived stem cell (MenSC) transplantation viadecellularized human amniotic membrane (DAM), for the promotion of skin excisional wound repair. The DAM wasseeded with MenSCs at the density of 3 9 104 cells/cm2 and implanted onto a rat’s 1.50 9 1.50 cm2 full-thicknessexcisional wound defect. The results of wound closure and histopathological examinations demonstrated that the MenSCseededDAM could significantly improve the wound healing compared with DAM-treatment. All in all, our data indicatedthat the MenSCs can be a potential source for cell-based therapies to regenerate skin injuries.

Phylogenetic Relationships among Seven Old Rose Species Grown in Iran Revealed by ISSR Markers

Zohreh Jabbarzadeh,Morteza Khosh-Khui,Hassan Salehi,Adel Saberivand 한국원예학회 2010 Horticulture, Environment, and Biotechnology Vol.51 No.3

Roses are among the most economically important ornamental crops. Inter-simple sequence repeat (ISSR) markers were used to study phylogenetic relationships among 7 rose species. Among 9 ISSR primers, 6 primers showed acceptable polymorphism and amplified 66 bands of which 50 were polymorphic. Banding patterns were transformed into binary data of presence-absence and matrices were processed using NTSYS pc 2.02 software program. The dendrogram was constructed using Jaccard coefficient and UPGMA algorithm. The 7 rose species were classified into 3 major groups with within-group similarity values of >0.58. Group 1 included R. banksiae; group 2, R. canina, R. chinensis, R. damascena, R. moschata and R. hybrida; and group 3, R. foetida. None of the species used in this study clustered within group 1, indicating that there is no direct relationship between these species. The species that were placed with R. hybrida in group 2 are the ancestors of R. hybrida and because of other species except these four species have contributed to the gene pool of modern garden roses, 59% similarity between these roses in this study was expected. In this phylogenetic tree R. foetida clusters in the lower part of the tree showed 49% similarity with group 2.

Molecular analysis of genetic diversity, population structure, and phylogeny of wild and cultivated tulips (Tulipa L.) by genic microsatellites

Ali Pourkhaloee,Morteza Khosh-Khui,Paul Arens,Hassan Salehi,Hooman Razi,Ali Niazi,Alireza Afsharifar,Jaap van Tuyl 한국원예학회 2018 Horticulture, Environment, and Biotechnology Vol.59 No.6

Tulip ( Tulipa L.) is one of the most important ornamental geophytes in the world. Analysis of molecular variability of tulipsis of great importance in conservation and parental lines selection in breeding programs. Of the 70 genic microsatellites, 15highly polymorphic and reproducible markers were used to assess the genetic diversity, structure, and relationships among280 individuals of 36 wild and cultivated tulip accessions from two countries: Iran and the Netherlands. The mean valuesof gene diversity and polymorphism information content were 0.69 and 0.66, respectively, which indicated the high discriminatorypower of markers. The calculated genetic diversity parameters were found to be the highest in wild T . systolaStapf (Derak region). Bayesian model-based STRU CTU RE analysis detected fi ve gene pools for 36 germplasms whichcorresponded with morphological observations and traditional classifi cations. Based on analysis of molecular variance, toconserve wild genetic resources in some geographical locations, sampling should be performed from distant locations toachieve high diversity. The unweighted pair group method with arithmetic mean dendrogram and principal component analysisplot indicated that among wild tulips, T . systola and T . micheliana Hoog exhibited the closest relationships with cultivatedtulips. Thus, it can be assumed that wild tulips from Iran and perhaps other Middle East countries played a role in the originof T . gesneriana , which is likely a tulip species hybrid of unclear origin. In conclusion, due to the high genetic variabilityof wild tulips, they can be used in tulip breeding programs as a source of useful alleles related to resistance against stresses.

The Effects of Natural Zeolite on Vegetative growth, Flower and Physiological Characteristics of African Marigold (Tagetes erecta L. ‘Queen’)

Nazari Farzad,Khosh-Khui Morteza,Eshghi Saeid,Salehi Hassan 한국원예학회 2007 Horticulture, Environment, and Biotechnology Vol.48 No.4

This research was conducted to investigate the effects of natural zeolite on vegetative growth, flower and physiological characteristics of African marigold (Tagetes erecta L. 'Queen'). The experiment was performed as a completely randomized design with six treatments (2, 4, 6, 8 and 10 g zeolite/kg soil mixture along with control) and 5 replications. Results indicated that zeolite decreased leaching of nitrogen, potassium, phosphorus, calcium, and magnesium due to increase in cation exchange capacity (CEC) of soil mixture. Application of zeolite in the medium resulted in significant increase in photosynthetic rate, mesophyll efficiency, water use efficiency, chlorophyll content, fresh and dry weights of roots and shoots, leaf area and flower length, but did not significantly affect flower diameter and stomatal conductance. It was concluded that zeolite had a pronounced effect on vegetative growth, flower and physiological characteristics of African marigold. It my be recommended that zeolite being used, as an amendment to soil of ornamental plants such as African marigold to decrease nutrient leaching and irrigation interval.

Evaluation of MT1XT20 Single Quasi-Monomorphic Mononucleotide Marker for Characterizing Microsatellite Instability in Persian Lynch Syndrome Patients

Farahani, Najmeh,Nikpour, Parvaneh,Emami, Mohammad Hassan,Hashemzadeh, Morteza,Zeinalian, Mehrdad,Shariatpanahi, Seyed Shervin,Salehi, Rasoul Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.9

Background: Colorectal malignancies with high microsatellite instability (MSI-H), either hereditary (Lynch syndrome) or sporadic, demonstrate better prognosis and altered response to 5FU chemotherapy. It is now recommended to perform MSI testing for all new cases of colorectal cancer regardless of being categorized as hereditary or sporadic. For MSI detection, immunohistochemistry or PCR-based protocols using a cohort of various sets of STR markers are recommended. Here we aimed to evaluate a simplified protocol using just a single STR marker, MT1XT20 mononucleotide repeat, for detection of MSI in Lynch syndrome patients. A Promega five-marker MSI testing panel and immunohistochemistry (IHC) were used as the gold standard in conjunction with MT1XT20. Materials and Methods: Colorectal patients with a positive history of familial cancers were selected by evaluating medical records. Based on Amsterdam II criteria for Lynch syndrome 20 families were short listed. DNA was extracted from formalin fixed paraffin embedded tumour and adjacent normal tissues resected from the index case in each family. Extracted DNA was subjected to MT1XT20 mononucleotide marker analysis and assessment with a commercially available five marker MSI testing kit (Promega, USA). IHC also was performed on tissue sections and the results were compared with PCR based data. Results: Eight (40%), seven (35%) and five (25%) cases were MSI positive using with the Promega kit, IHC and MT1XT20, respectively. Among the markers included in Promega kit, BAT26 marker showed instability in all 8 samples. NR24 and NR21 markers showed instability in 7 (87.5%), and BAT25 and MONO 27 in 6 (75%) and 5 (62.5%). Conclusions: Although MT1XT20 was earlier reported as a valid standalone marker for MSI testing in CRC patients, we could not verify this in our Iranian patients. Instead BAT26 among the markers included in Promega MSI testing kit showed instability in all 8 MSI-H CRC samples. Therefore, it seems BAT26 could act well as a single marker for MSI testing in Iranian CRC patients.