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        Phylogenetic Relationships among Seven Old Rose Species Grown in Iran Revealed by ISSR Markers

        Zohreh Jabbarzadeh,Morteza Khosh-Khui,Hassan Salehi,Adel Saberivand 한국원예학회 2010 Horticulture, Environment, and Biotechnology Vol.51 No.3

        Roses are among the most economically important ornamental crops. Inter-simple sequence repeat (ISSR) markers were used to study phylogenetic relationships among 7 rose species. Among 9 ISSR primers, 6 primers showed acceptable polymorphism and amplified 66 bands of which 50 were polymorphic. Banding patterns were transformed into binary data of presence-absence and matrices were processed using NTSYS pc 2.02 software program. The dendrogram was constructed using Jaccard coefficient and UPGMA algorithm. The 7 rose species were classified into 3 major groups with within-group similarity values of >0.58. Group 1 included R. banksiae; group 2, R. canina, R. chinensis, R. damascena, R. moschata and R. hybrida; and group 3, R. foetida. None of the species used in this study clustered within group 1, indicating that there is no direct relationship between these species. The species that were placed with R. hybrida in group 2 are the ancestors of R. hybrida and because of other species except these four species have contributed to the gene pool of modern garden roses, 59% similarity between these roses in this study was expected. In this phylogenetic tree R. foetida clusters in the lower part of the tree showed 49% similarity with group 2.

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        Interaction Effects of Chitosan, Benzyladenine, and Gibberellic Acid on in Vitro Proliferation of M26 Apple Rootstock

        Ziba Habibi Dastjerd,Zohreh Jabbarzadeh,Rasul Jalili Marandi 한국원예학회 2013 Horticulture, Environment, and Biotechnology Vol.54 No.6

        The present study was conducted to evaluate the proliferation ability of M26 apple rootstock on the MS medium supplemented with various concentrations of crab chitosan, gibberellic acid (GA3), and N6-benzyladenine (BA). Combination of 120 mg·L-1 chitosan and 0.5 mg·L-1 BA in the absence of GA3 was most suitable for the proliferation after 12 weeks. Without chitosan, total number of shoots increased by increasing BA concentration, but the shoots led to rosette and dense growth, developing tiny yellowish leaves with soft and watery tissues. Shoots taller than 3 cm were obtained by 0.1 or 0.3 mg·L-1 GA3 in combination with 120 mg·L-1 chitosan, but leaves were small with yellowish green color. These results indicated that chitosan can be used as a growth stimulator in combination with a minor amount of plant growth regulators to improve the proliferation rate of in vitro cultured M26 apple rootstock.

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