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( Jin Hua Chi ),( Hao Jin ),( Wen Yi Jiang ),( Sung Hee Lee ),( In Tae Hwang ),( Suck Chei Choi ),( Geom Seog Seo ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1
Background: High Mobility Group Box 1 protein (HMGB1) is a chromatin binding nucleus protein and has proinflammatory cytokine potential when released by damaged or necrosis cells during Inflammatory bowel dieases(IBD). This study is aimed to explore the association between ISQ and HMGB1 release in human intestinal cell. Methods: The protein expression of COX-2, fracnation of NF-κB and HMGB1, concentration of culture medium were analyzed by Western blot. Translocation of NF-κ B and HMGB1 were assessed by Fluorescence staining. Co-Immunoprecipitation assay for demonstrated acetyled HMGB1 in medium. HMGB1 and COX-2 mRNA level was analyzed by RT-PCR. Results: ISQ reduce TNF-a induced release of HMGB1 in extracellular and inhibit nucleus/cytosol translocation. ISQ also regulates NF-κB p65 translocation and inhibit the COX-2 expression which is the downstream of the NF-κB. Moreover ISQ suppressed the release of HMGB1 through reduction of the mRNA level and inhibit HMGB1 acetylation. Conclusions: In this study, all data evidence that released HMGB1 is a proinflammatory cytokine and leads to signaling cascades in inflammatory responses in human intestinal cell. These findings highlight the potential of ISQ for clinical applications in the treatment of intestinal inflammation including IBD.
Hao, Jin-Qing,Li, Hong,Woo, Hee-Gweon Wiley Subscription Services, Inc., A Wiley Company 2009 Journal of applied polymer science Vol.112 No.5
<P>A cholic acid (CA)-containing biodegradable hydrogel (PLA-PEG-PLA-co-MACAH) was synthesized from the photoinduced copolymerization of a CA-modified methacrylate monomer (MACAH), bearing a spacer of hexane-1,6-diol spacer between the methacryloyl and the cholanoate moieties, and a macromonomer (PLA-PEG-PLA-DA), bearing two acryloyl end groups derived from a poly(lactic acid)-b-poly(ethylene glycol)-b-poly(lactic acid) triblock copolymer. The structure of MACAH was confirmed by FTIR, <SUP>1</SUP>H-NMR, and MS. The hydrogel PLA-PEG-PLA-co-MACAH was characterized by scanning electron microscopy and X-ray diffraction. The experiment results showed that the swelling ratios of the hydrogels decreased with the increase of the CA fraction. The investigation on the in vitro degradation of the hydrogel showed that the CA-containing hydrogels degraded much slower than the hydrogels without CA component. The bioactivity of the synthesized hydrogels was assessed by the simulated body fluid method. The observed formation of hydroxyapatite on the scaffold of the hydrogels indicated that the hydrogels possess good bioactivity. © 2009 Wiley Periodicals, Inc. J Appl Polym Sci, 2009</P>
Jin, Li,Piao, Zhe Hao,Liu, Chun Ping,Sun, Simei,Liu, Bin,Kim, Gwi Ran,Choi, Sin Young,Ryu, Yuhee,Kee, Hae Jin,Jeong, Myung Ho John Wiley and Sons Inc. 2018 JOURNAL OF CELLULAR AND MOLECULAR MEDICINE Vol.22 No.3
<P><B>Abstract</B></P><P>Hypertension causes cardiac hypertrophy and leads to heart failure. Apoptotic cells are common in hypertensive hearts. Ca<SUP>2+</SUP>/calmodulin‐dependent protein kinase II (CaMKII) is associated with apoptosis. We recently demonstrated that gallic acid reduces nitric oxide synthase inhibition‐induced hypertension. Gallic acid is a trihydroxybenzoic acid and has been shown to have beneficial effects, such as anti‐cancer, anti‐calcification and anti‐oxidant activity. The purpose of this study was to determine whether gallic acid regulates cardiac hypertrophy and apoptosis in essential hypertension. Gallic acid significantly lowered systolic and diastolic blood pressure in spontaneously hypertensive rats (SHRs). Wheat germ agglutinin (WGA) and H&E staining revealed that gallic acid reduced cardiac enlargement in SHRs. Gallic acid treatment decreased cardiac hypertrophy marker genes, including atrial natriuretic peptide (<I>ANP</I>) and brain natriuretic peptide (<I>BNP</I>), in SHRs. The four isoforms, α, β, δ and γ, of <I>CaMKII</I> were increased in SHRs and were significantly reduced by gallic acid administration. Gallic acid reduced cleaved caspase‐3 protein as well as <I>bax</I>,<I> p53</I> and <I>p300 </I>mRNA levels in SHRs. <I>CaMKII</I> δ overexpression induced <I>bax</I> and <I>p53</I> expression, which was attenuated by gallic acid treatment in H9c2 cells. Gallic acid treatment reduced DNA fragmentation and the TUNEL positive cells induced by angiotensin II. Taken together, gallic acid could be a novel therapeutic for the treatment of hypertension through suppression of CaMKII δ‐induced apoptosis.</P>
Hao, Jian,Zou, Run-Hao,Liao, Rui-Jin,Yang, Li-Jun,Liao, Qiang,Zhu, Meng-Zhao The Korean Institute of Electrical Engineers 2018 Journal of Electrical Engineering & Technology Vol.13 No.4
Natural ester is a suitable substitute for mineral oil and has been widely used in AC transformer in many countries. In order to further application of natural ester in direct current (DC) equipment, it is needed to investigate its long term insulation property under DC condition. In this paper, a thermal ageing experiment was conducted for both mineral oil-paper and natural ester-paper insulation. The DC breakdown and space charge characteristics of insulation paper impregnated with natural ester and mineral oil was compared. Results show that the resistivity of the paper immersed in natural ester and mineral oil both increase as the ageing goes on. While insulation paper impregnated with natural ester has higher resistivity and DC breakdown voltage than the paper impregnated with mineral oil. The DC breakdown voltage for the oil impregnated insulation paper being DC pre-stressing is higher than that without pre-stressing. The average DC breakdown field strength difference between the test with pre-stressing and without pre-stressing clearly shows that there is an apparent enhancement effect for the homo-charge injection on the DC breakdown.
Hao Jin,Jing Ma,Yun Li,Ming Zhao 보안공학연구지원센터 2015 International Journal of Multimedia and Ubiquitous Vol.10 No.2
The distributed fusion filtering problem is studied for multi-rate sampling stochastic singular linear systems with multiple sensors and stochastic multiplicative noises. The system is described at the highest sampling rate and different sensors may have different lower sampling rates. The white noise in measurement matrix is introduced to describe the stochastic disturbance. Firstly, based on decomposition in canonical form, the original singular system is transformed into fast and slow two subsystems. For the two reduced-order subsystems, the local filters (LFs) are given based on the “dummy” random variables. The cross-covariance matrices between any two local filtering errors are derived. Further, the distributed fusion filter weighted by matrices (FFWM) is obtained for the original singular system based on the well-known fusion algorithm in the linear minimum variance sense. Simulation example verifies the correctness and feasibility of the proposed algorithm.
Hao Jin,Young-Ran Song,Chan-Mi Lee,Seon-Hye Lee,Sang-Ho Baik 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
Pediococcus acidilactici M76 (PA-M76) showed outstanding probiotic properties with high tolerance in acidic GIT environments, broad antimicrobial activity, and high adhesion capability in the intestinal tract of Caenorhabditis elegans. PA-M76 treatment resulted in significant increases of pro-inflammatory cytokine mRNA expression in macrophages. PA-M76 produced 1.62 g/L EPS under optimal lactic acid fermentation conditions. Lactic acid fermentation of BRE by PA-M76 did not significantly affect the total anthocyanin and flavonoid content, except for a significant increase in total polyphenol content compared to non-fermented BRE (NfBRE). However, fBRE exhibited increased DPPH radical scavenging activity, linoleic acid peroxidation inhibition rate, and ABTS scavenging activity of fBRE compared to NfBRE. Among the 28 compounds identified in the GC-MS analysis, esters were present as the major groups. The total concentration of volatile compounds was higher in fBRE than that in NfBRE. However, the undesirable flavor of terpenes decreased. PA-M76 might be useful for preparing functionally enhanced fermented beverages with a higher antioxidant activity of EPS and enhanced flavors.
GRIM-19 Expression and Function in Human Gliomas
Jin, Yong-Hao,Jung, Shin,Jin, Shu-Guang,Jung, Tae-Young,Moon, Kyung-Sub,Kim, In-Young The Korean Neurosurgical Society 2010 Journal of Korean neurosurgical society Vol.48 No.1
Objective : We determined whether the expression of GRIM-19 is correlated with pathologic types and malignant grades in gliomas, and determined the function of GRIM-19 in human gliomas. Methods : Tumor tissues were isolated and frozen at $-80^{\circ}C$ just after surgery. The tissues consisted of normal brain tissue (4), astrocytomas (2), anaplastic astrocytomas (2), oligodendrogliomas (13), anaplastic oligodendrogliomas (11), and glioblastomas (16). To profile tumor-related genes, we applied RNA differential display using a $Genefishing^{TM}$ DEG kit, and validated the tumor-related genes by reverse transcription polymerase chain reaction (RT-PCR). A human glioblastoma cell line (U343MG-A) was used for the GRIM-19 functional studies. The morphologic and cytoskeletal changes were examined via light and confocal microscopy. The migratory and invasive abilities were investigated by the simple scratch technique and Matrigel assay. The antiproliferative activity was determined by thiazolyl blue Tetrazolium bromide (MTT) assay and FACS analysis. Results : Based on RT-PCR analysis, the expression of GRIM-19 was higher in astrocytic tumors than oligodendroglial tumors. The expression of GRIM-19 was higher in high-grade tumors than low-grade tumors or normal brain tissue; glioblastomas showed the highest expression. After transfection of GRIM-19 into U343MG-A, the morphology of the sense-transfection cells became larger and more spindly. The antisensetransfection cells became smaller and rounder compared with wild type U343MG-A. The MTT assay showed that the sense-transfection cells were more sensitive to the combination of interferon-$\beta$ and retinoic acid than U343MG-A cells or antisense-transfection cells; the antiproliferative activity was related to apoptosis. Conclusion : GRIM-19 may be one of the gene profiles which regulate cell death via apoptosis in human gliomas.
Hao Jin,Shuo Yu,Shunhua Zhou,Junhua Xiao 대한토목학회 2019 KSCE JOURNAL OF CIVIL ENGINEERING Vol.23 No.2
Shield tunnel lining is mainly composed of prefabricated segment, accurate description of longitudinal seam joint mechanics is important for design and analysis of shield tunnel segmental linings, however, due to its stress state is complex and internal force changes show the characteristics of nonlinear, there is no more rapid and accurate calculation model to analysis the mechanics. In the view of the problem of the entity unit simulating bolt bending with low computing speed, the model of shield tunnel longitudinal seam that using the nonlinear spring to simulate bending bolt joint is proposed. Combined with longitudinal seam joint test, analysis the entity model and spring bolt model, the study concluded that: 1) the calculation results of spring bolt model are closer to the test results than entity unit model; and 2) calculation efficiency of spring bolt model bolt up 1 times than the physical model. Based on the spring bolt model, further study the relationship between viscous coefficient, friction coefficient and calculation efficiency, and analyzes the regularity of deformation and damage of shield tunnel connection.