http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Jin, Hanyong,Won, Miae,Shin, Eunkyoung,Kim, Hong-Man,Lee, Kangseok,Bae, Jeehyeon Elsevier 2017 Biochemical and biophysical research communication Vol. No.
<P><B>Abstract</B></P> <P>Pituitary gonadotropins are key hormones that orchestrate the growth and development of ovarian follicles. However, limited information is available on intra-ovarian factors that mediate the actions of gonadotropins. In this study, we identified that the early growth response 2 gene (<I>EGR2</I>) is a gonadotropin-inducible gene in granulosa cells of rats and humans. Analysis of consensus EGR-binding elements (EBEs) showed that the immediate early response 3 gene (<I>IER3</I>) is a novel transcriptional target gene of EGR2 as confirmed by the luciferase assay, electrophoretic mobility-shift assay (EMSA), chromatin immunoprecipitation (ChIP), and western blot analysis. Overexpression of EGR2 promoted survival of KGN human granulosa-derived cells in which IER3 acts as a mediator; knockdown of EGR2 induced death in KGN cells. Additionally, EGR2 was found to regulate the expression of myeloid cell leukemia 1 (MCL-1), which belongs to the BCL-2 family of proteins regulating cell survival. Thus, this study identified a novel signaling axis, comprised of gonadotropins-EGR2-IER3, which is important for the survival of granulosa cells during folliculogenesis.</P>
Ji-Hyun Yeom,Eunkyoung Shin,Hanyong Jin,Haifeng Liu,Yongyang Luo,Youngwoo Nam,Minkyung Ryu,Wooseok Song,Heeyoun Chi,Jeongkyu Kim,Kangseok Lee,Jeehyeon Bae 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.126 No.-
Antibodies are being increasingly used for therapeutic purposes due to their remarkable target specificityand affinity. However, currently available antibody therapies are restricted to target proteins in the outercell membrane or in the extracellular fluids because of the lack of technologies for effective intracellulardelivery of antibodies. Here, we report an efficient and versatile intracellular antibody delivery system. This system is based on gold nanoparticles (AuNPs) conjugated with DNA aptamers (Apt) against theFc region of IgG (AuNP-AptIgG), allowing to load any antibodies onto the AuNP-AptIgG by simple mixing. This AuNP-AptIgG-Ab platform was effective for cytosolic delivery of antibodies to clinically importantmutant proteins via scavenger receptors and caveolae-mediated endocytosis. Specifically, cancer cellsexpressing BRAFV600E, a variant of BRAF identified in numerous types of cancers, exhibited reduced cellviability by 70% when BRAFV600E antibodies were intracellularly delivered using the AuNP-AptIgG(AuNP-AptIgG-aBRAFV600E). In addition, subcutaneous injection of AuNP-AptIgG-aBRAFV600E into in vivoxenografted melanoma tumors expressing BRAFV600E resulted in both inhibition of proliferation andinduction of apoptosis, leading to tumor regression in mice. Thus, our findings indicate that the AuNPAptIgG-Ab system can serve as a promising platform for effective intracellular delivery of antibodies fortherapeutic purposes.
Epigenetic Activation of Tensin 4 Promotes Gastric Cancer Progression
Mirang Kim,Haejeong Heo,Hee-Jin Kim,Keeok Haam,Hyun Ahm Sohn,Yang-Ji Shin,Hanyong Go,Hyo-Jung Jung,Jong-Hwan Kim,Sang-Il Lee,Kyu-Sang Song,Min-Ju Kim,Haeseung Lee,Eun-Soo Kwon,Seon-Young Kim,Yong Sung 한국분자세포생물학회 2023 Molecules and cells Vol.46 No.5
Gastric cancer (GC) is a complex disease influenced by multiple genetic and epigenetic factors. Chronic inflammation caused by Helicobacter pylori infection and dietary risk factors can result in the accumulation of aberrant DNA methylation in gastric mucosa, which promotes GC development. Tensin 4 (TNS4), a member of the Tensin family of proteins, is localized to focal adhesion sites, which connect the extracellular matrix and cytoskeletal network. We identified upregulation of TNS4 in GC using quantitative reverse transcription PCR with 174 paired samples of GC tumors and adjacent normal tissues. Transcriptional activation of TNS4 occurred even during the early stage of tumor development. TNS4 depletion in GC cell lines that expressed high to moderate levels of TNS4, i.e., SNU-601, KATO III, and MKN74, reduced cell proliferation and migration, whereas ectopic expression of TNS4 in those lines that expressed lower levels of TNS4, i.e., SNU-638, MKN1, and MKN45 increased colony formation and cell migration. The promoter region of TNS4 was hypomethylated in GC cell lines that showed upregulation of TNS4. We also found a significant negative correlation between TNS4 expression and CpG methylation in 250 GC tumors based on The Cancer Genome Atlas (TCGA) data. This study elucidates the epigenetic mechanism of TNS4 activation and functional roles of TNS4 in GC development and progression and suggests a possible approach for future GC treatments.