Synthesis of 3-O-Sulfonated Heparan Sulfate Octasaccharides that Inhibit Herpes Simplex Virus Type 1 – Host Cell Interaction

Shang-Cheng Hung 한국당과학회 2012 한국당과학회 학술대회 Vol.2012 No.1

Cell surface carbohydrates play significant roles in a number of biologically importantprocesses. Heparan sulfate (HS), for instance, is a ubiquitously distributed polysulfatedpolysaccharide that is involved, among others, in the initial step of herpes simplex virus type1 (HSV-1) infection. The virus interacts with cell surface HS to facilitate host cellattachment and entry. 3-O-Sulfonated HS was discovered to function as an HSV-1 entryreceptor. A complete understanding of these interactions necessitates the chemical synthesisof such oligosaccharides, but remains challenging. Here, we present a convenient approachfor the synthesis of two irregular 3-O-sulfonated HS octasaccharides making use of a keydisaccharide intermediate to acquire different building blocks for the oligosaccharide chainassembly. Despite substantial structural differences, the prepared 3-O-sulfonated sugarsblocked viral infection in a dosage-dependent manner with remarkable similarity.

Embedded-Ge source and drain in InGaAs/GaAs dual channel MESFET

Shang-Chao Hung,Qiuping Luan,Hau-Yu Lin,Shuguang Li,Shoou-Jinn Chang 한국물리학회 2013 Current Applied Physics Vol.13 No.8

We report the first demonstration of n-type IIIeV metal-semiconductor field-effect transistors (nMESFETs) with IV group material hetero-junction source and drain (S/D) technology. A selective epitaxial growth of germanium (Ge) in the recessed gallium arsenide (GaAs) S/D regions is successfully developed using ultra-high vacuum chemical vapor deposition (UHVCVD) system. The dual channel structure includes an additional 10-nm higher mobility n-In0.2Ga0.8As layer on n-GaAs channel and is introduced to further improve the device performance. The n-MESFET, combining embedded-Ge S/D with In0.2Ga0.8As/GaAs channel, exhibits good transfer properties with a drain current on/off ratio of approximately 103. Due to the small barrier height of Ti/In0.2Ga0.8As Schottky contact, a lattice-matched wide bandgap In0.49Ga0.51P dielectric layer is also integrated into the device architecture to build a higher electron Schottky barrier height (SBH) for gate leakage current reduction. The Ti/In0.49Ga0.51P/n-In0.2Ga0.8As Schottky diode shows a comparable leakage level to Ti/n-GaAs with 2 x 10-2 A/cm2 at a gate voltage of -2.0 V.

Clinical and Histologic Features of Patients with Biopsy-Proven Metabolic Dysfunction-Associated Fatty Liver Disease

( Shang-chin Huang ),( Hau-jyun Su ),( Jia-horng Kao ),( Tai-chung Tseng ),( Hung-chih Yang ),( Tung-hung Su ),( Pei-jer Chen ),( Chun-jen Liu ) 대한간학회 2021 Gut and Liver Vol.15 No.3

Background/Aims: Fatty liver disease is defined as a cluster of diseases with heterogeneous etiologies, and its definition continues to evolve. The novel conceptional criteria for metabolic dysfunction-associated fatty liver disease (MAFLD) were proposed in 2020 to avoid the exclusion of a certain subpopulation, but their evaluations have been limited. We aimed to examine and compare the clinical as well as histologic features of MAFLD versus nonalcoholic fatty liver disease (NAFLD) in patients with biopsy-proven hepatic steatosis. Methods: From January 2009 to December 2019, 175 patients with histology-proven hepatic steatosis and 10 with cryptogenic cirrhosis who were treated at National Taiwan University Hospital, Taipei, Taiwan, were enrolled. Patients were classified into different groups according to the diagnostic criteria of MAFLD and NAFLD. The clinical and histologic features were then analyzed and compared. Results: In total, 76 patients (41.1%) were diagnosed with both MAFLD and NAFLD, 81 patients (43.8%) were diagnosed with MAFLD alone, nine patients (4.9%) were diagnosed with NAFLD alone, and 19 patients (10.3%) were diagnosed with neither. Those with MAFLD alone exhibited a higher degree of disease severity regarding histology and laboratory data than those with NAFLD alone. Advanced fibrosis was associated with the presences of hepatitis B virus infection and metabolic diseases. Conclusions: The novel diagnostic criteria for MAFLD include an additional 38.9% of patients with hepatic steatosis and can better help identify those with a high degree of disease severity for early intervention than can the previous NAFLD criteria. (Gut Liver 2021;15:451-458)

Strong adsorption of arsenic species by amorphous zirconium oxide nanoparticles

Hang Cui,Shian Gao,Jian Ku Shang,Qi Li 한국공업화학회 2012 Journal of Industrial and Engineering Chemistry Vol.18 No.4

A novel oxide adsorbent of amorphous zirconium oxide (am-ZrO2) nanoparticles was synthesized by a simple hydrothermal process for effective arsenic removal from aqueous environment. Due to their high specific surface area (327.1 m2/g), large mesopore volume (0.68 cm3/g), and the presence of high affinity surface hydroxyl groups, am-ZrO2 nanoparticles demonstrated exceptional adsorption performance on both As(III) (arsenite) and As(V) (arsenate) without pre-treatment at near neutral condition. At pH ~ 7,the adsorption kinetic is fast and the adsorption capacity is high (over 83 mg/g for As(III) and over 32.4 mg/g for As(V), respectively). Under low equilibrium arsenic concentrations (Ce at 0.01 mg/L, the maximum contaminant level (MCL) for arsenic in drinking water), the amount of arsenic adsorbed by am-ZrO2 nanoparticles is over 0.92 mg/g for As(III) and over 5.2 mg/g for As(V), respectively. The adsorption mechanism of arsenic species onto am-ZrO2 nanoparticles was found to follow the inner-sphere complex mechanism. Testing with arsenic contaminated natural lake water confirmed the effectiveness of these am-ZrO2 nanoparticles in removing arsenic from natural water. The immobilized am-ZrO2 nanoparticles on glass fiber cloth demonstrated an even better arsenic removal performance than dispersed am-ZrO2nanoparticles in water, paving the way for their potential applications in water treatment facility to treat arsenic contaminated water body without pre-treatment. 2012 The Korean Society of Industrial and Engineering Chemistry. Published by Elsevier B.V. All rights reserved.

Direct comparison of biopsy techniques for hepatic malignancies

( Shang-chin Huang ),( Ja-der Liang ),( Shih-jer Hsu ),( Tzu-chan Hong ),( Hung-chih Yang ),( Jia-horng Kao ) 대한간학회 2021 Clinical and Molecular Hepatology(대한간학회지) Vol.27 No.2

Background/Aims: The core needle biopsy (CNB), fine needle aspiration cytology (FNAC) and touch imprint cytology (TIC) are commonly used tools for the diagnosis of hepatic malignancies. However, little is known about the benefits and criteria for selecting appropriate technique among them in clinical practice. We aimed to compare the sensitivity of ultrasound-guided CNB, FNAC, TIC as well as combinations for the diagnosis of hepatic malignancies, and to determine the factors associated with better sensitivity in each technique. Methods: From January 2018 to December 2019, a total of 634 consecutive patients who received ultrasound-guided liver biopsies at the National Taiwan University Hospital was collected, of whom 235 with confirmed malignant hepatic lesions receiving CNB, FNAC and TIC simultaneously were enrolled for analysis. The clinical and procedural data were compared. Results: The sensitivity of CNB, FNAC and TIC for the diagnosis of malignant hepatic lesions were 93.6%, 71.9%, and 85.1%, respectively. Add-on use of FNAC or TIC to CNB provided additional sensitivity of 2.1% and 0.4%, respectively. FNAC exhibited a significantly higher diagnostic rate in the metastatic cancers (P=0.011), hyperechoic lesions on ultrasound (P=0.028), and those with depth less than 4.5 cm from the site of needle insertion (P=0.036). Conclusions: The sensitivity of CNB is superior to that of FNAC and TIC for the diagnosis of hepatic malignancies. Nevertheless, for shallow (depth <4.5 cm) and hyperechoic lesions not typical for primary liver cancers, FNAC alone provides excellent sensitivity. (Clin Mol Hepatol 2021;27:305-312)

Numerical Research on Suppression of Thermally Induced Wavefront Distortion of Solid-state Laser Based on Neural Network

Liu, Hang,He, Ping,Wang, Juntao,Wang, Dan,Shang, Jianli Optical Society of Korea 2022 Current Optics and Photonics Vol.6 No.5

To account for the internal thermal effects of solid-state lasers, a method using a back propagation (BP) neural network integrated with a particle swarm optimization (PSO) algorithm is developed, which is a new wavefront distortion correction technique. In particular, by using a slab laser model, a series of fiber pumped sources are employed to form a controlled array to pump the gain medium, allowing the internal temperature field of the gain medium to be designed by altering the power of each pump source. Furthermore, the BP artificial neural network is employed to construct a nonlinear mapping relationship between the power matrix of the pump array and the thermally induced wavefront aberration. Lastly, the suppression of thermally induced wavefront distortion can be achieved by changing the power matrix of the pump array and obtaining the optimal pump light intensity distribution combined using the PSO algorithm. The minimal beam quality β can be obtained by optimally distributing the pumping light. Compared with the method of designing uniform pumping light into the gain medium, the theoretically computed single pass beam quality β value is optimized from 5.34 to 1.28. In this numerical analysis, experiments are conducted to validate the relationship between the thermally generated wavefront and certain pumping light distributions.

Helicobacter pylori感染의 血淸學的 診斷法

尹熙相(Hee Shang Youn),白承哲(Seung-Chul Baik),李愚坤(Woo-Kon Lee),趙明濟(Myung-Je Cho),柳恒希(Hang-Hi Ryou),崔烋振(Hyu-Jin Choi),李光浩(Kwang-Ho Rhee) 大韓微生物學會 1990 大韓微生物學會誌 Vol.25 No.6

Transesterification of soybean oil to biodiesel by tin-based Brønsted-Lewis acidic ionic liquid catalysts

Xiaoxiang Han,Shang-Bin Liu,Wei Yan,Chin-Te Hung,Yanfei He,Pei-Hao Wu,Li-Li Liu,Shing-Jong Huang 한국화학공학회 2016 Korean Journal of Chemical Engineering Vol.33 No.7

A series of Brønsted-Lewis acidic ionic liquid (BLAIL) catalysts consisting of sulfonated ionic liquid [SO3H-pmim]Cl and Sn(II) chloride have been synthesized and exploited for catalytic transesterification of soybean oil with methanol to biodiesel. The structural and chemical properties of these [SO3H-pmim]Cl-xSnCl2 (x=0-0.8) catalysts were characterized by different analytical and spectroscopic techniques, such as FT-IR, TGA, and NMR. In particular, their acid properties were studied by solid-state 31P NMR using trimethylphosphine oxide as the probe molecule. The BLAIL catalysts were found highly efficient for transesterification reaction due to the introduction of Lewis acidity by SnCl2 in the initially Brønsted acidic [SO3H-pmim]Cl catalyst. The effects of three independent process variables on biodiesel yield were optimized by response surface methodology (RSM). Consequently, an excellent biodiesel yield of 98.6% was achieved under optimized reaction conditions over the BLAIL catalyst with SnCl2 loading (x) of 0.7.

Contact angles on quartz surfaces with micro-machined nanopillars

Ming-Tsung Hung,Sung-Chi Chen,Shang Yang,Shing-Lung Chen 한국생산제조학회 2014 한국생산제조시스템학회 학술발표대회 논문집 Vol.2014 No.9(2)

Assistive Control of a Surgical Robot Based on Bilateral Interacted Force Analysis

Ping-Lang Yen,Shang-Wei Hsu,Hang-Ting Lin,Ching-Heng Wang 제어로봇시스템학회 2012 제어로봇시스템학회 국제학술대회 논문집 Vol.2012 No.10

Interacted force between a robot, human and anatomical object in orthopedic surgery provides crucial information for safe and accurate bone cutting. When the robot plays the role of assisting the surgeon in the whole surgical procedure, the robot is trying to comply with the human intention according to various cutting conditions. The reaction force to the robot from the human is for indicating human intention, whereas the force from the anatomical object reflects the cutting condition. In this paper two impedance indices are defined by these two interacted forces and a surgical robot then decides whether assistive or resistant action is activated so that accuracy and safety of bone cutting can be achieved.