Behavioral Attraction of Two Parasitoids, Venturia canescens and Bracon hebetor, to Silk Extracts of a Host Plodia interpunctella

Ha, Dae-Myung,Choi, Sun-Ha,Shim, Jae-Kyoung,Jung, Duck-Oung,Song, Kyung-Sik,Nho, Si-Kab,Lee, Kyeong-Yeoll Korean Society of Applied Entomology 2006 Journal of Asia-Pacific Entomology Vol.9 No.3

Kairomonal activities of silk extracts of host Plodia interpunctella were determined by measuring the rates of behavioral responses of two parasitic wasps, Venturia canescens and Bracon hebetor. Silk of P. interpunctella larvae attracted both parasitic wasps but the cocoon silk of silkworm, Bombyx mori and the web silk of two-spotted spider mite, Tetranychus urticae did not. Silk components of P. interpunctella were extracted by using either hexane or methanol, and tested the rates of three serial responses of wasps; host location, antennal drumming and ovipositor probing behaviors. The patterns of each behavioral response were similar in two wasps. The rates of each response were increased at the higher concentrations of both extracts. Antennal drumming behavior was much more responsive to lower concentrations of both extracts than ovipositor probing behavior was. Furthermore, the rate of antennal drumming response was higher in hexane-extracts rather than methanol-extract in both wasps; V. canescens and B. hebetor for 20 and 17 times, respectively. However, ovipositor probing response was similar in two different extracts. Both extracts elicited 100% of antennal drumming response but ovipositor probing response was only 60 to 80% of all tested individuals. Our results were shown that silk extracts of host larvae elicited strong behavioral responses of two parasitic wasps and could be applied for practical application of parasitoids attraction in the biological control of agricultural pests.

Rat Growth-Hormone Release Stimulators from Fenugreek Seeds

Shim, Sang Hee,Lee, Eun Ju,Kim, Ju Sun,Kang, Sam Sik,Ha, Hyekyung,Lee, Ho Young,Kim, Chungsook,Lee, Je-Hyun,Son, Kun Ho WILEY-VCH Verlag 2008 CHEMISTRY AND BIODIVERSITY Vol.5 No.9

<P>Bioassay-guided fractionation of MeOH extract from fenugreek (Trigonella foenum-graecum L.) seeds resulted in the isolation of two rat growth-hormone release stimulators in vitro, fenugreek saponin I (1) and dioscin (9), along with two new, i.e., 2 and 3, and five known analogues, i.e., 4–8. The structures of the new steroidal saponins, fenugreek saponins I, II, and III (1–3, resp.), were determined as gitogenin 3-O-β-D-xylopyranosyl-(1→6)-β-D-glucopyranoside, sarsasapogenin 3-O-β-D-xylopyranosyl-(1→6)-β-D-glucopyranoside, and gitogenin 3-O-α-L-rhamnopyranosyl-(1→2)-β-D-glucopyranoside, respectively. Fenugreek saponin I (1) and dioscin (9) caused ca. 12.5- and 17.7-fold stimulation of release, respectively, of rat growth hormone from rat pituitary cells, whereas gitogenin (5) showed moderate activity. To our knowledge, this is the first study to demonstrate that steroidal saponins stimulate rat growth-hormone release in rat pituitary cells.</P>

척추 기기 고정술 후 발생한 후기 감염

심재익,김택선,이성종,이석하,이동기,유연식,김윤엽 대한척추외과학회 2000 대한척추외과학회지 Vol.7 No.1

연구계획 : 본 연구는 척추 기기 고정술후 발생한 후기 감염의 5예에 대한 후향적 분석을 시행하였다. 연구목적 : 척추 기기 고정술후 생긴 후기감염의 위험인자와 임상양상을 관찰하여 진단 및 치료를 하고 그 결과를 알아보고자 하였다. 대상 및 방법 : 저자들은 1993년 4월부터 1999년 6월까지 한국보훈병원 정형외과에서 척추 기기 고정술을 받았던 421명의 환자중 1년이상 추시 관찰이 가능하였던 374명을 대상으로 하여 후기감염으로 진단된 5예에 대하여 임상양상, 진단 및 치료결과를 후향적으로 분석하였다. 결과 : 전환자에서 악화되는 동통 및 이로 인한 파행이 나타났고, 동통은 진통제로 호전되지 않았다. 혈액검사에서는 ESR이 평균 44.8로, CRP가 평균 26.2로 증가되었다. 백혈구수는 1예에서만 경미한 증가소견을 보였다. 전환자에서 술후 4주에서 6주정도 항생제가 정맥 투여되었다. 방사선 소견에서는 나사못 주위의 골용해 간격이 3에서 7㎜로 평균 4.6㎜의 간격을 보였다. 5예중 2예에서 기기제거 및 변연절제술 후 항생제 투여를 하였고, 3예에서는 기기제거, 변연절제, 세척술과 동시에 기기 고정술을 재시행하였다. 수술 소견상 전례에서 모두 기기의 이완 및 나사못 주위로의 화농성 농양 소견을 보였으나, 2예에서 coagulase(-) staphylococcus와 S. epidermidids로 균이 동정되었다. 1예를 제외한 전례에서 동통의 감소가 있었고, 전례에서 혈액검사에서 ESR과 CRP의 수치가 정상화되었다. 평균 추시기간은 18.2개월이었다. 결론 : 척추 술후의 후기감염의 진단에는 임상적 추정이 상당히 중요하리라 사료된다. 정상적인 술후 회복 기간을 거친추 시작하여 악화되는 통증, ESR과 CRP의 증가, 방사선 소견상 나사못 주위의 골요해 소견을 진단의 실마리로 삼았다. 이러한 증상 및 소견이 나타나는 경우 후기 감염에 대한 가능성을 고려하여야 할 것으로 사료된다. 저자들의 경우 5예에서 술적 치료를 시행하여, 1예를 제외하고 만족스러운 결과를 얻었다. Study Design : A retrospective analysis of five cases of late spinal infection after spinal instrumentation and fusion. Objectives : These cases are reviewed to verify risk factors for late spinal infection after elective instrumentation and to manifest the treatment of this complication. Summary of Literature Review : Late spinal infection after elective spinal instrumentation and fusion are uncommon. The diagnosis is usually hard and requires much clinical suspicion. Materials and Methods : 5 cases were in total 374 patients of the author's cases. These cases are reviewed retrospectively. Results : All patients reported aggravated back pain. 4 patients had elevated erythrocyte sedimentation rates, aveaging 44.8㎜/hour and elevated C-reactive protein, averaging 26.2㎎/L. No distance foci of infection was identified. All patients got the raieolucent zone around screw fixation site, averaging 4.6㎜ width. The organisms were S. epidermidis in 1 case and coagulase(-) staphylococcus in 1 case. All cases were treated by operative method with debridement, instrument removal with or without revision and postoperative intravenous antibiotics. The average follow-up period was 18.2 months, one patient recurred back pain at 7 months after operation. Conclusion : The diagnosis of late infection after eletive spinal instrumentation and fusion requires high suspicion of clinical symptoms and signs. All except one were successfully treated by operative treatment.

Poster Session 2 : Oral Presentation ; Neurobiology : A novel mechanosensitive channel identified in sensory neurons

( Ha Won Cho ),( Sang Sung Kim ),( Young Duk Yang ),( Won Sik Shim ),( Mi Sook Kim ),( Byung Moon Kim ),( Uh Taek Oh ) 한국생화학분자생물학회 (구 한국생화학회) 2005 62회 KSBMB Annual Meeting in 2005 Vol.- No.-

Poster Session 2 : A novel mechanosensitive channel identified in sensory neurons

( Ha Won Cho ),( Sang Sung Kim ),( Young Duk Yang ),( Won Sik Shim ),( Mi Sook Kim ),( Byung Moon Kim ),( Uh Taek Oh ) 한국생화학분자생물학회 (구 한국생화학회) 2005 생화학분자생물학회 춘계학술발표논문집 Vol.2005 No.-

LL-37 inhibits serum amyloid A-induced IL-8 production in human neutrophils

Ha Young Lee,Sang Doo Kim,Jae Woong Shim,Sun Young Lee,Jeanho Yun,Yoe-Sik Bae 생화학분자생물학회 2009 Experimental and molecular medicine Vol.41 No.5

Serum amyloid A (SAA) has been regarded as an important mediator of inflammatory responses. The effect of several formyl peptide receptor-like 1 (FPRL1) ligands on the production of IL-8 by SAA was investigated in human neutrophils. Among the ligands tested, LL-37 was found to specifically inhibit SAA-induced IL-8 production in transcriptional and post-transcriptional levels. Since SAA stimulated IL-8 production via ERK and p38 MAPK in human neutrophils, we tested the effect of LL-37 on SAA induction for these two MAPKs. LL-37 caused a dramatic inhibition of ERK and p38 MAPK activity, which is induced by SAA. LL-37 was also found to inhibit SAA-stimulated neutrophil chemotactic migration. Further, the LL-37-induced inhibitory effect was mediated by FPRL1. Our findings indicate that LL-37 is expected to be useful in the inhibition of SAA signaling and for the development of drugs against SAA-related inflammatory diseases. Serum amyloid A (SAA) has been regarded as an important mediator of inflammatory responses. The effect of several formyl peptide receptor-like 1 (FPRL1) ligands on the production of IL-8 by SAA was investigated in human neutrophils. Among the ligands tested, LL-37 was found to specifically inhibit SAA-induced IL-8 production in transcriptional and post-transcriptional levels. Since SAA stimulated IL-8 production via ERK and p38 MAPK in human neutrophils, we tested the effect of LL-37 on SAA induction for these two MAPKs. LL-37 caused a dramatic inhibition of ERK and p38 MAPK activity, which is induced by SAA. LL-37 was also found to inhibit SAA-stimulated neutrophil chemotactic migration. Further, the LL-37-induced inhibitory effect was mediated by FPRL1. Our findings indicate that LL-37 is expected to be useful in the inhibition of SAA signaling and for the development of drugs against SAA-related inflammatory diseases.

Cardiovascular findings of Hutchinson-Gilford syndrome : A Doppler and two-dimensional echocardiographic study

Ha, Jong-Won,Shim, Won-Heum,Chung, Nam-Sik Yonsei University, College of Medicine 1993 Yonsei medical journal Vol.34 No.4

First Korean Case of 16p11.2 Duplication Syndrome Diagnosed by Chromosomal Microarray Analysis

Shim, Ye Jee,Park, So Yun,Jung, Nani,Kang, Seok Jin,Kim, Heung Sik,Ha, Jung-Sook Interdisciplinary Society of GeneticGenomic Medici 2019 Journal of interdisciplinary genomics Vol.1 No.1

A 10-year and 5 month-old girl with developmental delay, intellectual disability, attention deficit hyperactivity disorder, poor weight gain, and microcephaly was transferred to our pediatric clinic for genetic evaluation. Her height was within the 5-10th percentile, and her weight was under the 3rd percentile. On the social maturity scale, her developmental status was scored as 3 years 9 months for social age, and the social quotient was 35.98. A chromosomal microarray analysis was performed and the microduplication at chromosome 16p was observed: arr[GRCh37] 16p11.2 (29580020_30190029)${\times}3$. Currently, the patient is diagnosed with Grade 2 intellectual disability and is attending a computerized cognitive rehabilitation class twice weekly. In addition, nutritional support and growth follow up are also ensured in the Pediatric Gastrointestinal and Endocrinology clinic.

이분 슬개골내에 발생한 결절성 통풍 - 1례 보고 -

심재천,홍기도,하성식 대한슬관절학회 2000 대한슬관절학회지 Vol.12 No.2

Gout is a metabolic disorder manifested by hyperuricemia due to genetic or acquired diseases. The pathognomic lesion is the tophus which fine crystals of monosodium urate are deposited in articular cartilage, synovia, tendon sheaths and other periarticular structures, epiphyseal bone, subcutaneous layers of the skin, and interstitial areas of the kidney. Frequent sites of tophaceous deposit are the external ear and subcutaneous deposits in the finger tips, palms and soles. Tophaceous gout involving the space of bipartite patella is very unusual and has not been reported before in Korea. So, we report a case of 38 year old male patient who presented with tophaceous gout involving the space of bipartite patella with review of literatures.

Sub-50 nm template fabrications for nanoimprint lithography using hydrogen silsesquioxane and silicon nitride.

Shim, Jae Yeob,Baek, Kyu-Ha,Park, Kun-Sik,Shin, Hong-Sik,No, Kwang-Soo,Lee, Kijun,Do, Lee-Mi American Scientific Publishers 2010 Journal of nanoscience and nanotechnology Vol.10 No.5

<P>The sub-50 nm templates are successfully fabricated using hydrogen silsesquioxane (HSQ) and silicon nitride on silicon substrate. The HSQ template is directly patterned by e-beam direct writing. The cured HSQ pattern is used for the template of nanoimprint process. The silicon nitride template is reactively ion etched by ZEP resist mask pattern which is prepared by e-beam direct writing using ZEP resist. The line widths of HSQ templates and ZEP patterns after developments are between 22-30 nm and 24-30 nm, respectively. The line width of silicon nitride templates without performing descum is same as that of the ZEP pattern but shows a rough surface. When plasma descum was performed before RIE, the line width of silicon nitride templates increased from 27 nm to 35 nm and has a clean surface. The HSQ template fabrication results in this study will be promise for sub-nm imprint process.</P>