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Protein kinase A-dependent phosphorylation of B/K protein
Chin, Hemin,Choi, Sung-Ho,Jang, Yoon-Seong,Cho, Sung-Min,Kim, Ho-Shik,Lee, Jeong-Hwa,Jeong, Seong-Whan,Kim, In-Kyung,Kim, Grace J,Kwon, Oh-Joo Springer Science and Business Media LLC 2006 Experimental and molecular medicine Vol.38 No.2
<P>We have previously isolated a novel protein 'B/K' that contains two C2-like domains. Here, we report the isolation and mRNA distribution of a human B/K isoform, and protein kinase A (PKA)-dependent phosphorylation of the B/K protein. The 1.5 kb human B/K cDNA clone exhibits 89% and 97% identities with rat B/K in the sequences of nucleotide and amino acid, respectively. Human B/K isoform encodes a 474 amino acid protein and shows structural features similar to the rat counterpart including two C2 domains, three consensus sequences for PKA, absence of a transmembrane region, and conservation of the N-terminal cysteine cluster. On Northern and dot blot analyses, a 3.0 kb B/K transcript was abundantly present in human brain, kidney, and prostate. Among the brain regions, strong signals were observed in the frontal and temporal lobes, the hippocampus, the hypothalamus, the amygdala, the substantia nigra, and the pituitary. Recombinant B/K proteins containing three consensus sites for PKA was very efficiently phosphorylated in vitro by PKA catalytic subunit. B/K protein which was overexpressed in LLC-PK1 cells was also strongly phosphorylated in vivo by vasopressin analog DDAVP, and PKA-specific inhibitor H 89 as well as type 2 vasopressin receptor antagonist specifically suppressed DDAVP-induced B/K phosphorylation. These results suggest that B/K proteins play a role as potential substrates for PKA in the area where they are expressed.</P>
Lee, Sang Kyu,Lee, Dong Ju,Jeong, Hemin,Bista, Sudeep R.,Kang, Mi Jeong,Lee, Eung Seok,Son, Jong Keun,Nam, Doo Hyun,Chang, Hyeun Wook,Lee, Seung Ho,Jahng, Yurngdong,Jeong, Tae Cheon Taylor Francis 2007 Journal of toxicology and environmental health. Pa Vol.70 No.15
<P> To determine a possible role of glutathione (GSH) conjugation in 1,3-dibromopropane (1,3-DBP)-induced hepatotoxicity and immunotoxicity, female BALB/c mice were treated orally with 1,3-DBP. Based on the liquid chromatography/electrospray ionization-tandem mass spectrometry (LC/ESI-MS) analyses, two forms of S-bromopropyl GSH were observed at m/z 427.9 and 429.9 in the positive ESI spectrum with a retention time of 5.29 and 5.23 min, respectively. Following single treatment of mice with 150, 300 or 600 mg/kg 1,3-DBP for 12 hr, the amount of S-bromopropyl GSH was detected maximally in liver homogenates at 600 mg/kg 1,3-DBP. Hepatic GSH levels were significantly decreased by treatment with 1,3-DBP. In a time course study, production of S-bromopropyl GSH rose maximally 6 hr after treatment and decreased gradually thereafter. The liver weights were significantly increased by treatment with 600 mg/kg 1,3-DBP. When mice were treated orally with 600 mg/kg 1,3-DBP for 12 hr, the activities of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were increased by 365- and 83-fold. In addition, oral 1,3-DBP significantly suppressed the antibody response to a T-dependent antigen at 600 mg/kg 1,3-DBP. 1,3-DBP elevated hepatic levels of malondialdehyde and suppressed the activities of some hepatic enzymes involved in anti-oxidation. Taken together, the formation of GSH conjugate with 1,3-DBP may deplete cellular GSH and, subsequently, produce hepatotoxicity and immunotoxicity via damage to the cellular anti-oxidative system.</P>
Sun Hee Hyun,Tae Won Jeon,Sang Kyu Lee,Chun Hwa Kim,Young Min Seo,Ju Hyun Kim,Hemin Jeong,Mi Jeong Kang,Jae Sung Lee,Tae Cheon Jeong 한국독성학회 2007 Toxicological Research Vol.23 No.2
Paecilomyces tenuipes (PT), one of the Ascomycetes family, has been used for medicinal purposes due to its broad pharmacological activities. The present study was undertaken to investigate the hepatoprotective effects of PT water extracts against CCl₄-induced hepatotoxicity in primary cultures of adult rat hepatocytes. When the extract of PT was directly added into the culture medium at 1, 2, and 5 mg/ml, the extracts not only reduce the CCl₄-induced elevation of aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase, and lipid peroxide, but also protect cultured hepatocytes from CCl₄-induced reduction of reduced glutathione, glutathione reductase, glutathione- S-transferase, glutathione peroxidase, catalase and superoxide dismutase. In addition, the effects of PT water extracts on cytochrome P450 enzymes were relatively marginal, indicating that the hepatoprotective effects of PT extract against CCl₄-induced toxicity might not be due to the inhibition of CCl₄ activation. In conclusion, the PT extracts were effective in protecting against CCl₄- induced hepatotoxicity in hepatocyte cultures, at least in part, by scavenging free radicals, and by modulating enzyme systems involved in cellular oxidative stress.
재난안전 분야 공무원의 직무만족에 대한 영향요인: 직무위험과 직무압박, 직무스트레스, 그리고 내재적 동기의 영향을 중심으로
정지수 ( Jisu Jeong ),최혜민 ( Hemin Choi ) 서울대학교 한국행정연구소 2020 行政論叢 Vol.58 No.3
본 연구는 재난안전 분야의 공무원이 불확실한 직무의 위험과 압박 속에서 업무를 수행하고 있기 때문에 이러한 특성을 고려하여 직무만족에 미치는 영향요인을 고려하였다. 이를 위해 재난안전분야 공무원의 업무특성인 직무위험과 직무압박에 초점을 두고 직무스트레스를 함께 고려하여 직무만족에 미치는 영향을 분석하였다. 추가적으로 내재적 동기의 조절효과를 고려하여 정책적 방향성을 제시하였다. 분석결과 직무위험과 직무압박은 직무스트레스와 직무만족에 부정적 영향을 미치는 것으로 나타났다. 하지만, 내재적 동기는 직무스트레스가 직무만족에 미치는 부정적 영향을 상쇄하는 것으로 분석되었다. 재난안전 분야 공무원의 직무환경 자체를 변화시키는 것은 어려움이 있지만 직무위험과 직무압박을 줄일 수 있도록 하는 조직차원의 관리 노력이 반드시 필요하다. 또한, 변화에 소요되는 시간과 비용의 문제를 고려할 때 내재적 동기부여 요인을 통한 방식을 현실적 대안으로서 고려해볼 수 있을 것이다. 재난안전 분야 공무원의 근무환경과 역량은 치안경쟁력을 높이는 핵심요소라는 점에서도 본 연구의 함의가 있다. This study aims to identify what factors negatively affect job satisfaction. In particular, the group of interest included street-level bureaucrats such as police officers, fire fighters, and civil servants in disaster management who work in disaster environments that are physically dangerous and hazard generating or in administrative support work for disasters. The study explores empirically how disaster-related civil servants are able to manage their job pressure to enhance job satisfaction by using covariance structural analysis. The results show that job hazard and pressure negatively affect job stress and job satisfaction. Interestingly, however, it was found that intrinsic motivation can work as a moderator to reduce job stress, eventually leading to increased job satisfaction. In conclusion, job pressure, job risk (physically dangerous work), and internal job motivation can be utilized as key personnel management tools and need be considered in terms of personnel policy in order to improve job stress and job satisfaction. The findings of this study also provide policy implications for what needs to be prepared for public officials in a post-COVID-19 era.
The Effects of Rutaecarpine on the Pharmacokinetics of Acetaminophen in Rats
이상규,Sudeep R. Bista,Hemin Jeong,Dong Hyeon Kim,강미정,장영동,정태천 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.12
Rutaecarpine, an alkaloid originally isolated from the unripe fruit of Evodia rutaecarpa, has been shown to be anti-inflammatory as it inhibits cyclooxygenase-2. It induces the activities of hepatic CYP 1A2, 2B, and 2E1 in rats. A possible interaction between rutaecarpine and acetaminophen (APAP) was investigated in male Sprague Dawley rats in the present study. When 25 mg/kg APAP was intravenously administered concurrently with 80 mg/kg rutaecarpine, the area under the curve of APAP in plasma was significantly decreased when compared to that of APAP alone. When the rats were pre-treated orally with 40 and 80 mg/kg rutaecarpine for 3 days, the % value of Cmax and area under the curve of acetaminophen-sulfate conjugate were significantly decreased to 56.4% and 61.7% of the vehicle control group, respectively. These results suggest that rutaecarpine might cause changes in the pharmacokinetic parameters of APAP in rats.