Detection of Sulfated Glycosaminoglycan of Human Fetal Tooth Germs by HID-TCH-SP Method

Gyeung Ju Park,Chong Heon Lee 대한구강악안면병리학회 2005 대한구강악안면병리학회지 Vol.29 No.5

rt is well known that glycosaminoglycans are 01' fllndamental importance to the processes 01' morphogenesis and cytodifferentiati on dllring the teeth development , With HlD-TCH-SP(High - iron diamine-thiocarbohydrazide-silvel protcinntc) , s lllfatcd glycosaminoglycans such as chondroitin sulfate and heparan slllfate have been localized a t the III trastrllctural level i n a wide variety of tlssues The pmpose of this stlldy were to examine slllfated glycosaminoglycan at llltrastrllctllral level fo 1' the phase of morphogenesis and cytodifferentiation of hllma n fetal tooth germs, and to detect the protein expression of slllfated glycosaminoglycan by immunoslot blot Human tooth gerll1s fl'Oll1 the a lveolar bone of twenty still born fetuses we1'e fixed in a mixtllre of 2% gllltaraldehyde/1% forma ldehyde, The 미 t 1'athin sections were stained witb HID-TCH-SP and were treated with 0, 05% solution of t esticular hyaluronidase to identify the histochemical properties 01' tbe HID-TCH-SP stain deposits , For semi quantitative protein assay , immllnoslot blot was done Sulfated glycocongugated deposits were localized in DEJ, peri tubular dentin , and mantle dentin matrix‘ enamel prism sheath , interrod area , and enamel matnx Heparan sll l띠 te deposits i n DEJ resisted to testicular hyalllronidase treatment prior to HID-TCH-SP staining, Immunoslot blot s howed that• chondroitin sulfate was detected higher in enamel and dentin extract, while heparan sulfate was relatively expressed in enamel and dentin extract, but rarely expressed in enamel or dentin extract It suggest ecl that chonclroi tin and hepa ran slllfate woulcl play an important role in the formation of DEJ, while chondroitin sulfate would in the clevelopll1ent of enamel prism sheath, enamel matrix, and mantle 01' peritublllar clentin of human fetal tooth germs,

Functional improvement of hemostatic dressing by addition of recombinant batroxobin

Seon, Gyeung Mi,Lee, Mi Hee,Kwon, Byeong-Ju,Kim, Min Sung,Koo, Min-Ah,Kim, Dohyun,Seomun, Young,Kim, Jong-Tak,Park, Jong-Chul Elsevier 2017 Acta Biomaterialia: structure-property-function re Vol.48 No.-

<P><B>Abstract</B></P> <P>Although a number of natural materials have been used as hemostatic agents, many substances do not act quickly enough. Here, we created a novel dressings using collagen and chitosan with recombinant batroxobin (r-Bat) to promote faster and more effective hemostasis. We hypothesized that r-Bat would promote synergetic blood coagulation because it contains a blood coagulation active site different than those of collagen and chitosan. Our results suggest that each substances can maintain hemostatic properties while in the mixed dressings and that our novel hemostatic dressings promotes potent control of bleeding, as demonstrated by a whole blood assay and rat hemorrhage model. In a rat femoral artery model, the scaffold with a high r-Bat concentration more rapidly controlled excessive bleeding. This novel dressings has enormous possible for rapidly controlling bleeding and it improves upon the effect of collagen and chitosan used alone. Our novel r-Bat dressings is a possible candidate for improving preoperative care and displays promising properties as an absorbable agent in hemostasis.</P> <P><B>Statement of Significance</B></P> <P>Despite the excellent hemostatic properties of collagen and chitosan pads, they reported to brittle behavior and lack sufficient hemostatic effect within relevant time. Therefore, we created a novel pad using collagen and chitosan with recombinant batroxobin (r-Bat). r-Bat acts as a thrombin-like enzyme in the coagulation cascade. Specifically, r-Bat, in contrast to thrombin, only splits fibrinopeptide A off and does not influence other hemostatic factors or cells, which makes it clinically useful as a stable hemostatic agent. Also the materials in the pad have synergetic effect because they have different hemostatic mechanisms in the coagulation cascade. This report propose the novel hemostatic pad isreasonable that a great potential for excessive bleeding injury and improve effects of natural substance hemostatic pad.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Recombinant batroxobin-coated nonwoven chitosan as hemostatic dressing for initial hemorrhage control

Seon, Gyeung Mi,Lee, Mi Hee,Kwon, Byeong-Ju,Kim, Min Sung,Koo, Min-Ah,Seomun, Young,Kim, Jong-Tak,Kim, Tae Hee,Park, Jong-Chul Elsevier 2018 INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES Vol.113 No.-

<P><B>Abstract</B></P> <P>The choice of hemostat is determined by the situation and the degree of hemorrhage. One common hemostat, the nonwoven dressing, is easy to handled and controls severe bleeding on wider wounds. In this study, chitosan-based nonwoven dressings with recombinant batroxobin (rBat) were used as efficacious hemostatic dressing agents. Hemostatic agents need to absorb blood quickly in the early stages of blood coagulation cascade to rapidly and effectively control of excessive hemorrhages. To date, most studies of hemostatic agents focused on a single material and hemostats composed of multiple materials have not been studied sufficiently. Thus, we made a chitosan dressing coated with rBat and investigated the microstructure, mechanical properties, hemostatic efficacy, and clotting properties of the coated dressing. Our results showed that the rBat had a synergetic effect on chitosan that improved blood coagulation. Furthermore, the dressing had excellent bleeding control in an Sprague-Dawley (SD) rat femoral artery hemorrhage model. In conclusion, hemostasis can be improved by combining a chitosan-based nonwoven dressing with other agents, and rBat-coated chitosan-based nonwoven dressings have enormous potential to improve blood coagulation.</P>

CuII-M(M=Zn, Al 및 Fe) 산화-환원반응에서 활성 금속 및 음이온의 효과

박진희(Park, Jin-Hee),류경주(Rhu, Gyeung-Ju),김유빈(Kim, You-bin),박지현(Park, Ji-Hyeun),지유진(Gee, You-Jin),김봉곤(Kim, Bong-Gon) 한국과학영재교육학회 2018 과학영재교육 Vol.10 No.1

연구의 목적은 중학교 과학영재학생들의 R & E 프로그램으로, Cu (II) -M (M=Zn, Al, Fe)의 산화-환원반응에서 생성물의 형상과 반응속도에 미치는 구리(II) 이온의 농도, 활성 금속 (M) 및 음이온(spectator ion)의 영향에 대한 연구를 수행하는데 있다. 본 연구를 수행한 결과, 다음과 같은 결론을 얻었다. 첫째, CuSO4-Zn 산화-환원반응에서 0.5 M 황산구리 수용액 보다 진한 농도와 표면적이 큰 아연 분말을 사용하였을 때, 환원 생성물은 빠르게 붉은색 구리로 환원됨을 확인 하였다. 불균일 반응계인 Cu(II)-M의 산화-환원반응은 반응물의 농도가 증가하면 구리의 핵형성 속도가 증가하므로 초기 형성된 검은색 구리가 빠르게 붉은색으로 변화되는 것을 알 수 있었다. 산화-환원반응의 진행에 따른 생성물의 형상 변화는 0.2 M CuSO4 수용액에서 가장 쉽게 확인할 수 있었다. 둘째, 환원 생성물인 구리의 형상은 사용하는 활성 금속의 종류에 따라 다른 것을 확인하였다. 반응성이 큰 아연(Zn)과 알루미늄(Al)은 반응초기에 검은색 구리가 석출되지만, 철(Fe)을 사용하면, 철 표면에 환원된 구리가 도금되는 현상을 쉽게 관찰할 수 있었다. 이처럼 철을 사용하면 새로운 지적 호기심은 감소되지만 형상에 따른 색변화에 따른 학생들의 오개념 형성을 방지할 수 있을 것으로 생각된다. 셋째, 활성 금속(M)의 종류에 따른 Cu(II) -M 산화-환원 반응의 반응속도는 Zn > Al > Fe 순으로 얻어졌다. 이는 환원된 구리의 핵형성 속도가 수소기체의 생성과 관련이 있고, 환원된 구리의 흡착속도 차이를 의미한다. 넷째, 음이온에 의한 Cu(II)-Zn 치환 반응의 반응 속도는 Cl- > SO42- > NO3- 순서로 얻어졌다. 질산 구리(NO3-)와 금속 아연을 반응시켰을 경우, 초기 생성물은 다른 음이온과 같이 검은색 생성물이 얻어졌으나, 2시간 후 다공성의 녹청색 생성물을 확인할 수 있었다. 이 프로그램의 특징은 페트리 접시를 이용한 산화-환원 반응의 실험으로 거시적 현상을 저 비용과 친환경적으로 학생들이 화학 변화를 직접 관찰할 수 있기 때문에, 과학 영재 학생의 지적 호기심과 탐구력을 자극하고 과제 집중력을 높일 수 있었다. 또한, 이 프로그램의 부분적 수정으로 고등학교 과학 영재교육에 적용될 것으로 기대된다. The aims of in this study is to investigate the effects of the reactants concentration, kinds of active metal (M) and the anion (spectator ion) on the morphology and the rate constants of Cu(II)-M’substitution reaction as the R&E programs of science (chemistry) gifted students. As a result of this study, the following conclusions were obtained. First, in the CuSO4-Zn substitution reaction, it was confirmed that most of the product was red copper when using an aqueous solution of copper sulfate that is thicker than 0.5 M or using a zinc powder having a large surface area. As the concentration of the reactants increases, the nucleation rate of copper is increased and red copper is obtained. However, the concentration at which the change of metal surface was observed was 0.2 M CuSO4 aqueous solution. Second, it was confirmed that the morphology of copper as a reduction product varies depending on the kinds of the active metal. Although zinc (Zn) that generates hydrogen gas has black copper precipitate, iron (Fe) has a morphology and color similar to that of metallic copper. Third, the reaction rate of Cu(II)-M substitution reaction according to the kind of active metal(M) was shown in order of Zn, Al, and Fe. This indicates that the rate of nucleation of the reduced copper is related to the generation of hydrogen gas and the adsorption capacity of copper reduced to the surface of the active metal. Fourth, the reaction rate of Cu(II)-Zn substitution reaction by anions was decreased by Cl-, SO42-, and NO3- order. However, in the case of NO3-, the product was found to be a mixture of various types. The experimental methods of the redox reaction using Petri dishes are economical, environmentally friendly, and can directly observe the chemical changes as macroscopic phenomena. Also, applying this program to middle school students, we were able to stimulate intellectual curiosity and inquiry ability of science gifted students, and to increase task concentration ability. We expect that some modifications of this program will be applicable to high school science gifted education.

그루버 알고리즘 적용을 위한 LEA 양자 회로 최적화

장경배 ( Kyung Bae Jang ),김현준 ( Hyun Jun Kim ),박재훈 ( Jae Hoon Park ),송경주 ( Gyeung Ju Song ),서화정 ( Hwa Jeong Seo ) 한국정보처리학회 2021 정보처리학회논문지. 컴퓨터 및 통신시스템 Vol.10 No.4

양자 알고리즘과 양자 컴퓨터는 우리가 현재 사용하고 있는 많은 암호들의 안전성을 깨뜨릴 수 있다. 그루버 알고리즘을 n-bit 보안레벨을 가지는 대칭키 암호에 적용한다면 보안레벨을 (n/2)-bit까지 낮출 수 있다. 그루버 알고리즘을 적용하기 위해서는 오라클 함수에 대칭키 암호가양자 회로로 구현되어야 하기 때문에 대상 암호를 양자 회로로 최적화하는 것이 가장 중요하다. 이에 AES 또는 경량 블록암호를 양자 회로로 구현하는 연구들이 최근 활발히 진행되고 있다. 본 논문에서는 국산 경량 블록암호 LEA를 양자 회로로 최적화하여 구현 하였다. 기존의 LEA 양자회로 구현과 비교하여 양자 게이트는 더 많이 사용하였지만, 큐빗을 획기적으로 줄일 수 있었으며 이러한 트레이드오프 문제에 대한 성능 평가를 수행하였다. 마지막으로 제안하는 LEA 양자 회로에 그루버 알고리즘을 적용하기 위한 양자 자원들을 평가하였다. Quantum algorithms and quantum computers can break the security of many of the ciphers we currently use. If Grover’s algorithm is applied to a symmetric key cipher with n-bit security level, the security level can be lowered to (n/2)-bit. In order to apply Grover’s algorithm, it is most important to optimize the target cipher as a quantum circuit because the symmetric key cipher must be implemented as a quantum circuit in the oracle function. Accordingly, researches on implementing AES(Advanced Encryption Standard) or lightweight block ciphers as quantum circuits have been actively conducted in recent years. In this paper, korean lightweight block cipher LEA was optimized and implemented as a quantum circuit. Compared to the previous LEA quantum circuit implementation, quantum gates were used more, but qubits were drastically reduced, and performance evaluation was performed for this tradeoff problem. Finally, we evaluated quantum resources for applying Grover’s algorithm to the proposed LEA implementation.

Effective stacking and transplantation of stem cell sheets using exogenous ROS-producing film for accelerated wound healing

Koo, Min-Ah,Hee Hong, Seung,Hee Lee, Mi,Kwon, Byeong-Ju,Mi Seon, Gyeung,Sung Kim, Min,Kim, Dohyun,Chang Nam, Ki,Park, Jong-Chul Elsevier 2019 ACTA BIOMATERIALIA Vol. No.

<P><B>Abstract</B></P> <P>Extensive skin loss caused by burns or diabetic ulcers may lead to major disability or even death. Therefore, cell-based therapies that enhance skin regeneration are clinically needed. Previous approaches have been applied the injections of cell suspensions and the implantation of biodegradable three-dimensional scaffolds seeded cells. However, these treatments have limits due to poor localization of the injected cells and insufficient delivery of oxygen and nutrients to cells. Recently, cell sheet-based tissue engineering has been developed to transplant cell sheets, which are cell-dense tissues without scaffolds. Because cell density is one of the important factors for improving the therapeutic effect of cell transplantation, transplanting layered cell sheet constructs can promote the recovery of tissue function and tissue regeneration compared with a single cell sheet. Thus, this study designed ROS-induced cell sheet stacking method with newly fabricated hematoporphyrin-incorporated polyketone film (Hp-PK film) to enhance cell sheet delivery efficiency and application in wound healing. We have demonstrated the therapeutic effect of a multi-layered mesenchymal stem cell sheets onto a full-thickness wound defect in nude mice. Consequentially, three-layered cell sheets transplanted and stacked by ROS-induced method promoted angiogenesis and skin regeneration at the wound site. Thus, our strategy based on Hp-PK film, which allows for easy stacking and transplantation of cell sheets, could be applied to enhance tissue regeneration.</P> <P><B>Statement of Significance</B></P> <P>We herein report exogenous ROS-induced cell sheet stacking method with newly fabricated hematoporphyrin-incorporated polyketone film (Hp-PK film) to enhance cell sheet transplantation efficiency and application in wound healing. Although there are several ways to stack-up cell sheets, all of these methods have limitations in transplanting the cell sheet directly to the target site. The method is simple and takes a relatively short time compared to previously reported methods for stacking and transplanting cell sheets. Thus, our study will provide a scientific impact because the method of applying exogenous ROS generated from Hp-PK film on cell detachment can transplant the cell sheet through a process of putting a cell sheet-cultured film on the lesion, irradiating with light, and then removing only the film.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Influence of Biomimetic Materials on Cell Migration

Kim, Min Sung,Lee, Mi Hee,Kwon, Byeong-Ju,Koo, Min-Ah,Seon, Gyeung Mi,Kim, Dohyun,Hong, Seung Hee,Park, Jong-Chul Kluwer Academic Publishers 2018 Advances in experimental medicine and biology Vol.1064 No.-

Controlled Delivery of Extracellular ROS Based on Hematoporphyrin-Incorporated Polyurethane Film for Enhanced Proliferation of Endothelial Cells

Koo, Min-Ah,Kim, Bong-Jin,Lee, Mi Hee,Kwon, Byeong-Ju,Kim, Min Sung,Seon, Gyeung Mi,Kim, Dohyun,Nam, Ki Chang,Wang, Kang-Kyun,Kim, Yong-Rok,Park, Jong-Chul American Chemical Society 2016 ACS APPLIED MATERIALS & INTERFACES Vol.8 No.42

<P>The principle of photodynamic treatment (PDT) involves the administration of photosensitizer (PS) at diseased tissues, followed by light irradiation to produce reactive oxygen species (ROS). In cells, a moderate increase in ROS plays an important role as signaling molecule to promote cell proliferation, whereas a severe increase of ROS causes cell damage. Previous studies have shown that low levels of ROS stimulate cell growth through PS drugs-treating PDT and nonthermal plasma treatment. However, these methods have side effects which are associated with low tissue selectivity and remaining of PS residues. To overcome such shortcomings, we designed hematoporphyrin-incorporated polyurethane (PU) film induced generation of extracellular ROS with singlet oxygen and free radicals. The film can easily control ROS production rate by regulating several parameters including light dose, PS dose. Also, its use facilitates targeted delivery of ROS to the specific lesion. Our study demonstrated that extracellular ROS could induce the formation of intracellular ROS. In vascular endothelial cells, a moderated increase in intracellular ROS also stimulated cell proliferation and cell cycle progression by accurate control of optimum levels of ROS with hematoporphyrin-incorporated polymer films. This modulation of cellular growth is expected to be an effective strategy for the design of next-generation PDT.</P>

Suppression of T24 human bladder cancer cells by ROS from locally delivered hematoporphyrin-containing polyurethane films

Kim, Dohyun,Lee, Mi Hee,Koo, Min-Ah,Kwon, Byeong-Ju,Kim, Min Sung,Seon, Gyeung Mi,Hong, Seung Hee,Park, Jong-Chul The Royal Society of Chemistry 2018 PHOTOCHEMICAL AND PHOTOBIOLOGICAL SCIENCES Vol.17 No.6

<P>Systemic injection of a photosensitizer is a general method in photodynamic therapy, but it has complications due to the unintended systemic distribution and remnants of photosensitizers. This study focused on the possibility of suppressing luminal proliferative cells by excessive reactive oxygen species from locally delivered photosensitizer with biocompatible polyurethane, instead of the systemic injection method. We used human bladder cancer cells, hematoporphyrin as the photosensitizer, and polyurethane film as the photosensitizer-delivering container. The light source was a self-made LED (510 nm, 5 mW cm<SUP>−2</SUP>) system. The cancer cells were cultured on different doses of hematoporphyrin-containing polyurethane film and irradiated with LED for 15 minutes and 30 minutes each. After irradiating with LED and incubating for 24 hours, cell viability analysis, cell cycle analysis, apoptosis assay, intracellular and extracellular ROS generation study and western blot were performed. The cancer cell suppression effects of different concentrations of the locally delivered hematoporphyrin with PDT were compared. Apoptosis dominant cancer cell suppressions were shown to be hematoporphyrin dose-dependent. However, after irradiation, intracellular ROS amounts were similar in all the groups having different doses of hematoporphyrin, but these values were definitely higher than those in the control group. Excessive extracellular ROS from the intended, locally delivered photosensitizer for photodynamic treatment application had an inhibitory effect on luminal proliferative cancer cells. This method can be another possibility for PDT application on contactable or attachable lesions.</P>

배면개구형 집광시트의 자체 정렬 특성

박경주,김영규,최계훈,백상훈,황성기,곽진석,이종훈,권진혁,박이순,Park, Gyeung-Ju,Kim, Young-Gyu,Choi, Gye-Hun,Baik, Sang-Hoon,Hwang, Sung-Ki,Gwag, Jin-Seog,Yi, Jong-Hoon,Kwon, Jin-Hyuk,Park, Yi-Soon 한국광학회 2009 한국광학회지 Vol.20 No.5

액정표시장치의 고효율 백라이트 집광시트로 활용하기 위하여 배면 개구형 집광시트를 설계 및 분석하였다. Microlens array sheet, 피라미드 array sheet, 그리고 cone array sheet에 대해서 자체 정렬에 의한 배면 개구 형성 특성을 비교 분석한 결과, microlens array sheet가 가장 우수하였고, 다음으로 피라미드 array sheet였으며, cone array sheet가 가장 부적합하였다. Optical sheets with apertures on the opposite side of the substrate are designed and analyzed in order to use them as high efficiency light concentration sheets in LCD edge-lit backlight. Formation of apertures by self-aligning exposure were analyzed for the microlens array sheet, pyramid array sheet, and cone array sheet and the microlens array sheet showed the best performance for the formation of apertures by the self-aligning exposure.