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김은경 ( Eun-gyeong Kim ),김민경 ( Min-kyung Kim ),권현애 ( Hyun-ae Kwon ),윤도경 ( Do-kyung Youn ),구정헌 ( Jeong-heon Koo ),박소연 ( So-yeon Park ),이희근 ( Hui-geun Lee ),조명희 ( Myeong-hui Jo ),하도윤 ( Do-yun Hah ),김철호 ( 한국동물위생학회(구 한국가축위생학회) 2018 韓國家畜衛生學會誌 Vol.41 No.3
Salmonella Enteritidis (S. Enteritidis) are found in animals, humans, and environment. In addition, S. Enteritidis draws attention to the public health concerns due to carriage of antibiotic resistance traits. For these reasons, the prevalence and antibiotic resistance patterns of S. Enteritidis are significant issues with regard to public health. To address this issues, a total of 24 strains of S. Enteritidis from 164 samples collected from several slaughterhouses in Gyeong-Nam province in order for antibiotic resistance profiles. Subsequently, we characterized the genotyping by random amplification polymorphic DNA (RAPD)-PCR. As a result, very high level of resistance to protein synthesis inhibition antibiotics and most isolates were susceptible to others. Six random primers were used for RAPD-PCR to reveal genotypes of S. Enteritidis isolates. One of the primer, P1245, generated 147 distinct RAPD-PCR fragments ranging from 400∼3000 bp. The number of RAPD-PCR products ranged from 4 to 8 for this primer. The RAPD-PCR fragments could be placed these strains into 3 subgroups and 2 classes by UPGMA cluster analysis. Interestingly, several S. Enteritidis that isolated from different slaughterhouses showed same genotype. These results showed only limited genetic variation among the isolates, those were grouped into a few different patterns of antibiotic resistance.
Kim, Seong Gyeong,Jang, Sungho,Lim, Jae Hyung,Jeon, Byoung Seung,Kim, Jungyeon,Kim, Kyoung Heon,Sang, Byoung-In,Jung, Gyoo Yeol Elsevier 2018 Bioresource technology Vol.247 No.-
<P><B>Abstract</B></P> <P>The aim of this study is to demonstrate that rebalancing of metabolic fluxes at acetyl-CoA branch node can substantially improve the titer and productivity of hexanoic acid in recombinant <I>Escherichia coli</I> strains. First, a hexanoic acid-producing <I>E. coli</I> strain was constructed by expressing genes encoding β-ketothiolase (BktB) from <I>Cupriavidus necator</I> and acetyl-CoA transferase (ACT) from <I>Megasphaera</I> sp. MH in a butyric acid producer strain. Next, metabolic flux was optimized at the acetyl-CoA branch node by fine-tuning the expression level of the gene for acetyl-CoA acetyltransferase (AtoB). Four synthetic 5′-untranslated regions were designed for <I>atoB</I> using UTR Designer to modulate the expression level of the gene. Notably, the productivity of the optimized strain (14.7 mg/L/h) was the highest among recombinant <I>E. coli</I> strains in literature when using a similar inoculum size for fermentation. These results show that fine-tuning the expression level of <I>atoB</I> is critical for production of hexanoic acid.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Recombinant <I>E. coli</I> strains were constructed to produce hexanoic acid. </LI> <LI> The enzyme that catalyzes the last step of the hexanoic acid pathway was replaced. </LI> <LI> Precise flux rebalancing at the acetyl-CoA branch point was achieved. </LI> <LI> The productivity achieved was 8.7-fold higher than that in the parental strain. </LI> <LI> The productivity of the optimized strain was 14.7 mg/L/h. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Kim, Ha Gyeong,Kim, Chan Woo,Lee, Don Haeng,Lee, Jae-Seon,Oh, Eun-Taex,Park, Heon Joo MDPI AG 2019 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.20 No.18
<P>5-Fluorouracil (5-FU) is an important chemotherapeutic agent for the systemic treatment of colorectal cancer (CRC), but its effectiveness against CRC is limited by increased 5-FU resistance caused by the hypoxic tumor microenvironment. The purpose of our study was to assess the feasibility of using quinacrine (QC) to increase the efficacy of 5-FU against CRC cells under hypoxic conditions. QC reversed the resistance to 5-FU induced by hypoxia in CRC cell lines, as determined using ATP-Glo cell viability assays and clonogenic survival assays. Treatment of cells with 5-FU under hypoxic conditions had no effect on the expression of nuclear factor (erythroid-derived 2)-like 2 (Nrf2), a regulator of cellular resistance to oxidative stress, whereas treatment with QC alone or in combination with 5-FU reduced Nrf2 expression in all CRC cell lines tested. Overexpression of Nrf2 effectively prevented the increase in the number of DNA double-strand breaks induced by QC alone or in combination with 5-FU. siRNA-mediated c-Jun N-terminal kinase-1 (JNK1) knockdown inhibited the QC-mediated Nrf2 degradation in CRC cells under hypoxic conditions. The treatment of CRC xenografts in mice with the combination of QC and 5-FU was more effective in suppressing tumor growth than QC or 5-FU alone. QC increases the susceptibility of CRC cells to 5-FU under hypoxic conditions by enhancing JNK1-dependent Nrf2 degradation.</P>
Neuron-like differentiation of mesenchymal stem cells on silicon nanowires.
Kim, Hyunju,Kim, Ilsoo,Choi, Heon-Jin,Kim, So Yeon,Yang, Eun Gyeong RSC Pub 2015 Nanoscale Vol.7 No.40
<P>The behavior of mammalian cells on vertical nanowire (NW) arrays, including cell spreading and the dynamic distribution of focal adhesions and cytoskeletal proteins, has been intensively studied to extend the implications for cellular manipulations in vitro. Prompted by the result that cells on silicon (Si) NWs showed morphological changes and reduced migration rates, we have explored the transition of mesenchymal stem cells into a neuronal lineage by using SiNWs with varying lengths. When human mesenchymal stem cells (hMSCs) were cultured on the longest SiNWs for 3 days, most of the cells exhibited elongated shapes with neurite-like extensions and dot-like focal adhesions that were prominently observed along with actin filaments. Under these circumstances, the cell motility analyzed by live cell imaging was found to decrease due to the presence of SiNWs. In addition, the slowed growth rate, as well as the reduced population of S phase cells, suggested that the cell cycle was likely arrested in response to the differentiation process. Furthermore, we measured the mRNA levels of several lineage-specific markers to confirm that the SiNWs actually induced neuron-like differentiation of the hMSCs while hampering their osteogenic differentiation. Taken together, our results implied that SiNWs were capable of inducing active reorganization of cellular behaviors, collectively guiding the fate of hMSCs into the neural lineage even in the absence of any inducing reagent.</P>
Kim, Hee Jin,Chun, Byung Chul,Kwon, AmyM,Lee, Gyeong-Ho,Ryu, Sungweon,Oh, Soo Yeon,Lee, Jin Beom,Yoo, Se Hwa,Kim, Eui Sook,Kim, Je Hyeong,Shin, Chol,Lee, Seung Heon The Korean Academy of Tuberculosis and Respiratory 2015 Tuberculosis and Respiratory Diseases Vol.78 No.4
Background: The tuberculin skin test (TST) is the standard tool to diagnose latent tuberculosis infection (LTBI) in mass screening. The aim of this study is to find an optimal cut-off point of the TST+ rate within tuberculosis (TB) contacts to predict the active TB development among adolescents in school TB outbreaks. Methods: The Korean National Health Insurance Review and Assessment database was used to identify active TB development in relation to the initial TST (cut-off, 10 mm). The 7,475 contacts in 89 schools were divided into two groups: Incident TB group (43 schools) and no incident TB group (46 schools). LTBI treatment was initiated in 607 of the 1,761 TST+ contacts. The association with active TB progression was examined at different cut-off points of the TST+ rate. Results: The mean duration of follow-up was $3.9{\pm}0.9years$. Thirty-three contacts developed active TB during the 4,504 person-years among the TST+ contacts without LTBI treatment (n=1,154). The average TST+ rate for the incident TB group (n=43) and no incident TB group (n=46) were 31.0% and 15.5%, respectively. The TST+ rate per group was related with TB progression (odds ratio [OR], 1.025; 95% confidence interval [CI], 1.001-1.050; p=0.037). Based on the TST+ rate per group, active TB was best predicted at TST+ ${\geq}$ 16% (OR, 3.11; 95% CI, 1.29-7.51; area under curve, 0.64). Conclusion: Sixteen percent of the TST+ rate per group within the same grade students can be suggested as an optimal cut-off to predict active TB development in middle and high schools TB outbreaks.
An efficient nano-composite layer for highly transparent organic light emitting diodes
Kim, Gyeong Woo,Lampande, Raju,Boizot, Julien,Kim, Gyeong Heon,Choe, Dong Cheol,Kwon, Jang Hyuk The Royal Society of Chemistry 2014 Nanoscale Vol.6 No.7
We report highly transparent and low resistive new cathode structures, which basically consist of nano-composite layer/Ag/WO3 for transparent organic light-emitting diode (TOLED) applications. Our new cathode structure exhibits an extremely high transmittance of 91.2% at 550 nm, a low sheet resistance of 5.4 Omega square(-1), and excellent electron injection properties. Such a high transmittance along with a low resistivity of the fabricated new cathode could be explained by surface-modifying behavior with the generation of a nano-composite thin silver oxide layer during Ag deposition. Chemical interaction at the interface between the electron injection layer and the electron transport layer results in good electron injection properties in TOLEDs. The fabricated TOLEDs with our new cathode structures have a full device transmittance of 85-87% at 550 nm.
수질오염총량관리 단위유역 장기유황곡선 구축 -낙동강수계를 대상으로-
김경훈 ( Gyeong Hoon Kim ),권헌각 ( Heon Gak Kwon ),안정민 ( Jung Min Ahn ),김상훈 ( Sanghun Kim ),임태효 ( Tae Hyo Im ),신동석 ( Dong Seok Shin ),정강영 ( Kang-young Jung ) 한국환경과학회 2017 한국환경과학회지 Vol.26 No.8
For the development of flow duration curves for the management of 41 Total Maximum Daily Load (TMDL) units of the Nakdong River basin, first, an equation for estimating daily flow rates as well as the level of correlation (correlation and determination coefficients) was extrapolated through regression analysis of discrete (Ministry of Environment) and continuous (Ministry of Land, Infrastructure and Transportation) measurement data. The equation derived from the analysis was used to estimate daily flow rates in order to develop flow duration curves for each TMDL unit. By using the equation, the annual flow duration curves and flow curves, for the entire period and for each TMDL unit of the basin, were developed to be demonstrated in this research. Standard flow rates (abundant-, ordinary-, low- and drought flows) for major flow duration periods were calculated based on the annual flow duration curves. Then, the flow rates, based on percentile ranks of exceedance probabilities (5, 25, 50, 75, and 95%), were calculated according to the flow duration curves for the entire period and are suggested in this research. These results can be used for feasibility assessment of the set values of primary and secondary standard flow rates for each river system, which are derived from complicated models. In addition, they will also be useful for the process of implementing TMDL management, including evaluation of the target level of water purity based on load duration curves.