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Long-term outcomes of abdominal paraganglioma
Hye Ryeon Choi,Zeng Yap,Soon Min Choi,Sun Hyung Choi,Jin Kyong Kim,Cho Rok Lee,Jandee Lee,Jong Ju Jeong,Kee-Hyun Nam,Woong Youn Chung,Sang-Wook Kang 대한외과학회 2020 Annals of Surgical Treatment and Research(ASRT) Vol.99 No.6
Purpose: Paragangliomas (PGL) are rare neuroendocrine tumors derived from chromaffin cells of the autonomic nervous system. We aim to describe our experience and the long-term outcome of abdominal PGL over the last decade. Methods: A retrospective review of patients diagnosed with PGL in our hospital between November 2005 and June 2017 was conducted. All nonabdominal PGL were excluded and the clinicopathological features and long-term outcomes of the patients were analyzed. Results: A total of 46 patients were diagnosed with abdominal PGL. The average age of diagnosis was 55.4 years and there was no sex predilection. The average tumor size was 5.85 cm and they were predominantly located in the infrarenal position (50%). The mean follow-up period was 42 months (range, 1.8–252 months). All patients with metastases had Pheochromocytoma of the Adrenal Gland Scaled Score (PASS) of ≥4. One patient presented with synchronous metastases while 2 developed local recurrence and distant metastases. One presented with only local recurrence. One patient died 5 years after diagnosis. Conclusion: Abdominal PGL is a rare tumor with excellent long-term prognosis. Recurrence although uncommon, can occur decades after initial diagnosis. Long-term follow-up is therefore recommended for all patients with PGL, especially in patients with PASS of ≥4
Choi Hye Ryeon,Choi Sun Hyung,Hong Namki,Rhee Yumie,Kim Jin Kyong,Lee Cho Rok,Kang Sang-Wook,Lee Jandee,Jeong Jong Ju,Nam Kee-Hyun,Chung Woong Youn 대한의학회 2022 Journal of Korean medical science Vol.37 No.13
Background: Normocalcemic primary hyperparathyroidism (NPHPT) was first described in 2008. It is defined as consistently elevated serum parathyroid hormone (PTH) levels with normal serum calcium (sCa) concentration, after excluding secondary causes of PTH elevation. However, the exact definition and management strategy for NPHPT remain controversial. We retrospectively investigated the clinicopathological features and short-term outcomes of NPHPT patients. Methods: A total of 280 patients who were surgically indicated for primary hyperparathyroidism (PHPT) at the Yonsei Severance Medical Center between 2015 and 2019 were included. Patients were classified according to preoperative PTH, corrected sCa, and ionized calcium (iCa) levels as follows: typical primary hyperparathyroidism (TPHPT, elevated PTH, sCa, and iCa, n = 158) and NPHPT (elevated PTH, normal sCa, n = 122). Results: NPHPT was commonly seen in younger individuals (aged < 50 years, P = 0.025); nephrolithiasis and bone fractures were common. Preoperative PTH level was higher in the TPHPT group (P < 0.001). The NPHPT group had higher numbers of multiple parathyroid lesions (P = 0.004) that were smaller (P = 0.011). NPHPT patients were further divided into two subgroups according to iCa levels: the elevated (n = 95) and normal iCa (n = 27) groups. There was no significant difference between the two subgroups regarding symptoms and multiplicity of lesions. Conclusion: We found that NPHPT may be a heterogeneous disease entity of PHPT with high rates of multi-gland disease, which appears to be biochemically milder but symptomatic. Intraoperative PTH monitoring might help increase the surgery success rate. Moreover, the short-term outcomes of NPHPT after surgery did not differ from that of TPHPT.
Sequence Analysis of Phosphorylation Modification of Chicken Embryo Elongation Factor-2
KIM, Yeon Woong,KIM, Inchul,KIM, Choong Won,KANG, Kee Ryeon,KANG, Yoon-Se 경상대학교 유전공학연구소 1993 遺傳工學硏究所報 Vol.12 No.-
The proposed tyrosine phosphorylation consensus sequence of (R,K)X{2,3}X(D,E)X{2,3}Y*was searched in the amino acid sequence of chicken EF-2 which had been deduced from the nucleotide sequence. MOTIFS somputer program in GCG analysis package was used. We found two tyrosine phophorylation motifs, 366-KYRCELLY*-373 and 739-RLMEPIY*-745 present in the EF-2 molecule. CNBr cleavage of ^32P-radiolabeled EF-2 followed by tricine SDS-PAGE yielded a peptide digest with molecular weight of 5,000, which included one of the two tyrosine phosphorylation motifs, 366-KYRCELLY*-373. To isolate the peptide containing phosphotyrusine resudue, the purified EF-2 was digested with trypsin and the tryptic peprides were loaded on antiphosphotyrusine antibody affinity column. The bound fraction was further purified by thin layer electrophoresis and TLC on a precoated cellulose plate and FPLC.
Cellular Proliferation and Expression of Proto-Oncogenes in Chicken Embryonic Cells
KIM, YEON WOONG,KIM, CHOOG WON,SUNG, SOON KEE,KANG, KEE RYEON,KANG, YOON-SE 경상대학교 유전공학연구소 1988 遺傳工學硏究所報 Vol.7 No.-
Tyrosine protein kinase has been implicated in the mediation of cellular proliferation and development. Products of several viral oncogenes and growth factors have been shown to be associated with tyrosine kinase activity. Thus we investigated expression of tyrosine kinase-related proto-oncogenes during early chicken embryonic development (from 30 hr to 10 day). Each of four c-onc gene (i.e c-fos, c-src, c-abl, and c-fes/fps) showed characteristic expression depending on the time periods within 10 days, but level of c-erbB appeared very low compared to the other proto-oncogenes. Histone H2B gene was used as control.
Kim, Choong Won,Kim, Yeon Woong,Kang, Kee Ryeon,Eom, Mi-Ok,Jung, Eun Joo,Kim, Jong Chul,Ahn, Hong Joon,Kang, Yoon-Se 경상대학교 유전공학연구소 1992 遺傳工學硏究所報 Vol.11 No.-
Chicken elongation factor 2(EF-2) cDNA was isolated from a chicken intestine cDNA libary and the sequence was determined. The probe for the screening of library was prepared by polymerase chain reaction(PCR) using two degenerate oligonuckeotide primers encoding NH_2-terminal region (residue 9-14) of chicken EF-2 and a conserved GTP binding domain (residue 80-84) of hamster EF-2. The sequence of amplified 228 bp product was identical to that of hamster EF-2(residue 9-84). One of 5 positive clones, pCEK4, consisted of 3,144 nucleotides with 2,574 bp open reading frame coding for 858 amino acid reaidues. The M_r 95,361 of the protein calculated from the amino acid sequence agreed well with the molecular weight of chicken and other mammalian EF-2(95,000) measured by SDS-PAGE. Sequence identity between chicken and other mammalian EF-2s(human, hamster, and rat) was 97%, while the sequence of GTP-binding/-hydrolysis domain was 99% identical. This result indicates that EF-2 is one of the highly conserved proteins and its structure has not been changed significantly during evolution. When expression of EF-2 in various stage of chicken embryo development was estimated by measuring mRNA and protein of EF-2, the result showed that the levels of its EF-2 mRNA and protein levels were the highest in 3 day embryo and then decreased gradually.
KIM, CHOONG WON,KIM, YEON WOONG,KANG, KEE RYEON,JUNG, EUN JOO,KIM, JONG CHUL,KANG, YOON SE 경상대학교 유전공학연구소 1991 遺傳工學硏究所報 Vol.10 No.-
Five cDNA clones of elongation factor 2(EF-2) were isolated from chicken intestine cDNA library. All insert in λgt 10 expect one were found to be 3.2 kb. The size should fully encodes for chicken EF-2 with molecular mass of 95kDa. using one of the clones, designated pCEK4, we determined partial amino acid sequence of EF-2 protein. The sequence included NH_2-terminal 169 amino acids correaponding to the common motif of five regions found in GTP-binding proteins. Comparative sequence analysis of each domain among chicken and hamster EF-2 shpwed a striling homology. ADP-ribosylation of chicken EF-2 in the presence of diphtheria toxin and NAD^+ provided an indirect evidence that ADP-ribosylatable diphthamide, 2-[3-carboxyamide-3-(trimethyl ammonio)-propyl]histidine. Finally measurements of EF-2 in chicken embryo of early developmental stage suggest that expression of EF-2 is correlated with the extent of cell growth and development.