Lactobacillus salivarius Strain FDB89 Induced Longevity in Caenorhabditis elegans by Dietary Restriction

Yang Zhao,Liang Zhao,Xiaonan Zheng,Tianjiao Fu,Huiyuan Guo,Fazheng Ren 한국미생물학회 2013 The journal of microbiology Vol.51 No.2

In this study, we utilized the nematode Caenorhabditis elegans to assess potential life-expanding effect of Lactobacillus salivarius strain FDB89 (FDB89) isolated from feces of centenarians in Bama County (Guangxi, China). This study showed that feeding FDB89 extended the mean life span in C. elegans by up to 11.9% compared to that of control nematodes. The reduced reproductive capacities, pharyngeal pumping rate, growth, and increased superoxide dismutase (SOD)activity and XTT reduction capacity were also observed in FDB89 feeding worms. To probe the anti-aging mechanism further, we incorporated a food gradient feeding assay and assayed the life span of eat-2 mutant. The results demonstrated that the maximal life span of C. elegans fed on FDB89was achieved at the concentration of 1.0 mg bacterial cells/plate, which was 10-fold greater than that of C. elegans fed on E. coli OP50 (0.1 mg bacterial cells/plate). However, feeding FDB89 could not further extend the life span of eat-2mutant. These results indicated that FDB89 modulated the longevity of C. elegans in a dietary restriction-dependent manner and expanded the understanding of anti-aging effect of probiotics.

Invited Lectures ( 1998 . 11 . 12 -17 ) : Alkaloid from Stemona and Lyophyllum

(Yang Ye),(Wen Han Lin),(Guo Wei Qin),(Ren Sheng Xu) 한국응용약물학회 1998 학술대회 Vol.1998 No.1

Application of modified graphite felt as electrode material: a review

Yang Su,Na Chen,Hai‑lin Ren,Cheng‑wei Li,Li‑li Guo,Zhen Li,Xiao‑min Wang 한국탄소학회 2023 Carbon Letters Vol.33 No.1

Graphite felt is a felt-like porous material made of high-temperature carbonized polymers. It is widely used in electrode materials because of its good temperature resistance, corrosion resistance, large surface area and excellent electrical conductivity. In this paper, the surface functional group modification is of graphite felt electrodes (mainly nitrogen doping modification, nitrogen–sulfur or nitrogen–boron co-doping modification) and surface catalytic modification (metal/ion surface modification and metal oxide surface modification as Main). There are two main methods and research progresses to improve the performance of graphite felt electrodes, and the comprehensive performance of surface functional group-modified graphite felt electrodes and surface catalytically modified graphite felt electrodes are compared respectively. The results show that both surface functional group modification and surface catalytic modification can improve the comprehensive performance of graphite felt electrodes. In this paper, the future development direction of graphite felt activation modification is also prospected.

Partial Pole Assignment via Constant Gain Feedback in Two Classes of Frequency-domain Models

Guo-Sheng Wang,Guo-Zhen Yang,Guang-Ren Duan 대한전기학회 2007 International Journal of Control, Automation, and Vol.5 No.2

The design problem of partial pole assignment (PPA) in two classes of frequency-domain MIMO models by constant gain feedback is investigated in this paper. Its aim is to design a constant gain feedback which changes only a subset of the open-loop eigenvalues, while the rest of them are kept unchanged in the closed-loop system. A near general parametric expression for the feedback gain matrix in term of a set of design parameter vectors and the set of the closed-loop poles, and a simple parametric approach for solving the proposed problem are presented. The set of poles do not need to be previously prescribed, and can be set undetermined and treated together with the set of parametric vectors as degrees of design freedom provided by the approach. An illustrative example shows that the proposed parametric method is simple and effective.

Comparison of Gadobenate Dimeglumine and Gadopentetate Dimeglumine for Breast MRI Screening: a Meta-analysis

Yang, Xiao-Ping,Han, Yue-Dong,Ye, Jian-Jun,Chen, Gang,Luo, Ying,Ma, Hong-Xia,Yu, Xue-Wen,Niu, Juan-Qin,Ren, Fang-Yuan,Guo, You-Ming Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.12

Background: As a common and essential contrast medium at present, gadobenate dimeglumine has shown better performance than some other agents when applied to Breast Magnetic Resonance Imaging Screening (Breast MRI Screening). Nevertheless, reports on the diagnostic performance of these two mediums (gadobenate dimeglumine and gadopentetate dimeglumine) are not completely consistent. Objective: To assess the diagnostic value of gadobenate dimeglumine and gadopentetate dimeglumine for Breast MRI Screening in patients suffering from breast cancer and to provide more convinced evidence to guide clinical practice in terms of appropriate contrast agents. Data Sources and Review Methods: Original articles in English and Chinese published before January 2013 were selected from available databases (The Cochrane Library, PUBMED, EMBASE, Chinese Biomedical Literature Database, Chinese Scientific Journals Full-text Database, Chinese Journal Full-text). The criteria for inclusion and exclusion were based on the standard for diagnosis tests. Meta-Disc software (Version 1.4) was used for data analysis. Then, the area under curve (AUC) of SROC and the spearman rank correlation of sensitivity against (1-specificity) were calculated. Results: Total of 17 researches involving 1934 patients were included. The pooled sensitivity of gadobenate dimeglumine and gadopentetate dimeglumine were 0.99 (0.97, 1.00) and 0.93 (0.88, 1.00) respectively. The pooled specificity for these two contrast agents were 0.924 (0.902, 0.943) and 0.838 (0.817, 0.858) respectively, and the AUC of SROC curve were 0.9781 and 0.9215 respectively. Conclusions: Gadobenate dimeglumine can be regarded as a more effective and feasible contrast medium for Breast MRI Screening. At least 5% differences in diagnostic performance are usually considered as clinically relevant.

Research on optimization of milling performance of V-groove micro-texture ball-end milling cutter

Shucai Yang,Chaoyang Guo,Wei Ren 대한기계학회 2022 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.36 No.6

Titanium alloys generally have problems such as sticking, large cutting force, and poor heat dissipation during the cutting process., research shows that processing microtextures on the surface of the tool can effectively improve the above problems. Therefore, this paper designs the V-groove micro-textures based on the principle of bionics. Use simulation software to analyze the milling behavior of different V-groove micro-texture ball-end milling cutters for milling titanium alloys. Build a test platform to study the milling performance of the Vgroove micro-texture ball-end milling cutter for milling titanium alloys, obtain the prior choice range of V-groove micro-texture parameters, optimize the parameters based on the simulated annealing algorithm and conduct experimental verification. The results of the optimal tool parameters are that the opening angle of the V-groove micro-textures is 79°, the V-groove microtexture spacing is 170 μm, and the V-groove micro-texture width is 30 μm, the distance from blade of the V-groove micro-textures is 90 μm.

Study in the Mechanisms of Formation of Transfer Film under the Condition of Wear of Steel AISI1020 by Natural Rubber

DE-GUO WANG,SI-WEI ZHANG,REN-YANG HE,MING-YUAN LI 한국트라이볼로지학회 2002 한국트라이볼로지학회 학술대회 Vol.2002 No.10

The mechanisms of formation of transfer film under the condition of wear of Steel AISI1020 by natural rubber were investigated. The transfer film was observed and the formation mechanisms were clarified. The formation process of transfer film on the worn surface of the steel could be divided into two stages. Firstly, the adhesive layer emerged on the worn surface of the steel by adhesion of natural rubber, in which the macromolecular chains of natural rubber joined to the surface of the steel by Van der Waals' force. And then, the iron atom and metal oxide reacted with the macroradicals of natural rubber in the adhesive layer and produced Fe-polymer compound. As a result, the transfer film was formed on the worn surface of the steel. The transfer film was joined to the worn surface of the steel by the chemical bonds and electrostatic force.

Crystallographic Characterizations of Eutectic and Secondary Carbides in a Fe-12Cr-2.5Mo-1.5W-3V-1.25C Alloy

Jing Guo,Ligang Liu,Yunli Feng,Sha Liu,Xuejun Ren,Qingxiang Yang 대한금속·재료학회 2017 METALS AND MATERIALS International Vol.23 No.2

In this work, the morphology and structures of the eutectic and secondary carbides in a new high chromiumFe-12Cr-2.5Mo-1.5W-3V-1.25C designed for cold-rolling work roll were systematically studied. The precipitatedcarbides inside the grains and along the grain boundaries were investigated with optical microscope,scanning electron microscopy with energy dispersive spectroscopy, transmission electron microscopy andX-Ray diffraction. Selected area diffraction patterns have been successfully used to identify the crystal formationand lattice constants of the carbides with different alloying elements. The results show that the eutectic carbidesprecipitated contain MC and M2C distributed along the grain boundaries with dendrite feature. The compositionand crystal structure analysis shows that the eutectic MC carbides contain VC and WC with a cubic and hexagonalcrystal lattice structures respectively, while the eutectic M2C carbides predominantly contain V2C and Mo2Cwith orthorhombic and hexagonal crystal lattices respectively. The secondary carbides contain MC, M2C,M7C3 formed along the grain boundaries and their sizes are much larger than the eutectic carbides ones. The secondary M23C6 is much small (0.3-0.5μm) and is distributed dispersively inside the grain. Similar tothe eutectic carbides, the secondary carbides also contain VC, WC, V2C, and Mo2C. M7C3 is hexagonal(Fe,Cr)7C3, while M23C6 is indexed to be in a cubic crystal form.

Fe₃O₄ magnetic nanoparticles provide a novel alternative strategy for Staphylococcus aureus bone infection

Youliang, Ren,Jin, Yang,Jinghui, Zhang,Xiao, Yang,Lei, Shi,Dajing, Guo,Yuanyi, Zheng,Haitao, Ran,Zhongliang, Deng,Lei, Chu Techno-Press 2022 Advances in nano research Vol.13 No.6

Due to its biofilm formation and colonization of the osteocyte-lacuno canalicular network (OLCN), Staphylococcus aureus (S.aureus) implant-associated bone infection (SIABI) is difficult to cure thoroughly, and may occur recurrently subsequently after a long period dormant. It is essential to explore an alternative therapeutic strategy that can eradicate the pathogens in the infected foci. To address this, the polymethylmethacrylate (PMMA) bone cement and Fe3O4 nanoparticles compound cylinder were developed as implants based on their size and mechanical properties for the alternative magnetic field (AMF) induced thermal ablation, The PMMA mixed with optimized 2% Fe₃O₄ nanoparticles showed an excellent antibacterial efficacy in vitro. It was evaluated by the CFU, CT scan and histopathological staining on a rabbit 1-stage transtibial screw model. The results showed that on week 7, the CFU of infected soft tissue and implants, and the white blood cells (WBCs) of the PMMA+2% Fe₃O₄+AMF group decreased significantly from their controls (p<0.05). PMMA+2% Fe₃O₄+AMF group did not observe bone resorption, periosteal reaction, and infectious reactive bone formation by CT images. Further histopathological H&E and Gram Staining confirmed there was no obvious inflammatory cell infiltration, neither pathogens residue nor noticeably burn damage around the infected screw channel in the PMMA+2% Fe₃O₄+AMF group. Further investigation of nanoparticle distributions in bone marrow medullary and vital organs of heart, liver, spleen, lung, and kidney. There were no significantly extra Fe₃O₄ nanoparticles were observed in the medullary cavity and all vital organs either. In the current study, PMMA+2% Fe₃O₄+AMF shows promising therapeutic potential for SIABI by providing excellent mechanical support, and promising efficacy of eradicating the residual pathogenic bacteria in bone infected lesions.

Simulation of natural convection in an inclined polar cavity using a finite-difference lattice Boltzmann method

Fan Yang,Haicheng Yang,Yonghua Yan,Xueyan Guo,Ren Dai,Chaoqun Liu 대한기계학회 2017 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.31 No.6

Natural convection heat transfer in an inclined polar cavity was studied using a Finite-difference lattice Boltzmann method (FDLBM)based on a double-population approach for body-fitted coordinates. A D2G9 model coupled with the simplest TD2Q4 lattice model wasapplied to determine the velocity field and temperature field. For both velocity and temperature fields, the discrete spatial derivativeswere obtained by combining the upwind scheme with the central scheme, and the discrete temporal term is obtained using a fourth-orderRunge-Kutta scheme. Studies were carried out for different Rayleigh numbers and different inclination angles. The results in terms ofstreamlines, isotherms, and Nusselt numbers explain the heat transfer mechanism of natural convection in an inclined polar cavity due tothe change of Rayleigh number and inclination angle.