Evaluation of C. Albicans and S. Mutans adherence on different provisional crown materials

Gulsum Sayin Ozel,Mehmet Burak Guneser,Ozgur Inan,Ayce Unverdi Eldeniz 대한치과보철학회 2017 The Journal of Advanced Prosthodontics Vol.9 No.5

PURPOSE. Bacterial adhesion on provisional crown materials retained for a long time can influence the duration for which permanent prosthetic restorations can be healthily worn in the oral cavity. The aim of this study was to compare seven different commonly used provisional crown materials with regard to Streptococcus mutans and Candida albicans surface adhesion. MATERIALS AND METHODS. For each group, twenty specimens of the provisional fixed prosthodontic materials TemDent (Schütz), Imıdent (Imıcryl), Tab 2000 (Kerr), Structur Premium (Voco), Systemp (Ivoclar Vivadent), Acrytemp (Zhermack), and Takilon-BBF (Takilon) were prepared (diameter, 10.0 mm; height, 2.0 mm). Surface roughness was assessed by atomic force microscopy. Each group was then divided into 2 subgroups (n=10) according to the microbial suspensions used: S. mutans and C. albicans. The specimens were incubated at 37°C with S. mutans or C. albicans for seven days. Bacterial adherence on surfaces was assessed using the 2,3-bis[2-methyloxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxanilide (XTT) assay. RESULTS. S. mutans showed maximum adhesion to Structur, followed by Systemp, Acrytemp, Takilon, Tab 2000, Imident, and TemDent (P<.05). The highest vital C. albicans adhesion was noted on Takilon, followed by Imident and Tab 2000; the lowest adhesion was noted on Systemp (P<.05). CONCLUSION. The materials showed significant differences in the degree of bacterial adhesion. C. albicans showed higher surface adhesion than S. mutans on provisional crown and fixed partial denture denture materials.

Evaluation of abiotic stress tolerance and physiological characteristics of potato (Solanum tuberosum L. cv. Kennebec) that heterologously expresses the rice Osmyb4 gene

Gulsum Aydin,Huseyin Avni Oktem,Meral Yucel,Ming-Tsair Chan 한국식물생명공학회 2014 Plant biotechnology reports Vol.8 No.3

MYB transcription factors are involved indiverse biochemical and physiological processes, includinghormone signaling, defense, and stress responses. In thepresent study, we developed a transgenic potato (Solanumtuberosum L. cv. Kennebec) expressing the rice Osmyb4gene, which encodes the transcription factor MYB4. Thetransgene was under the control of either the constitutiveCaMV35S promoter or the stress-induced ArabidopsisCOR15a promoter. The potential involvement of MYB4 incertain physiological processes and the abiotic stressresponse in the potato was evaluated. The transgenic plantsdid not exhibit growth retardation, and they showed nosignificant difference (P\0.05) in tuber yield from that ofnon-transgenic wild-type plants. Although the chlorophylla and b as well as the anthocyanin contents of the sixtransgenic lines were similar to those of the wild type, thetransgenic line S2 presented a significantly higher carotenoidcontent. The total sugar contents of the lines S2 andM48 were significantly higher than that of the wild-typeplants. S2 and M48 were significantly more tolerant ofsalinity than the wild type, according to measured growthparameters. Transgenic plants grown under a high concentrationof boric acid (3 mM) exhibited greater survivalrates than non-transgenic control plants. On the other hand,the transgenic plants did not show an improvement infreezing tolerance. Overall, our results indicated thatMYB4 may affect diverse processes such as carotenoidbiosynthesis, sugar metabolism, and salinity tolerance inpotato, and that it may be an upstream regulatory elementof these processes.

Evaluation of C. Albicans and S. Mutans adherence on different provisional crown materials

Ozel, Gulsum Sayin,Guneser, Mehmet Burak,Inan, Ozgur,Eldeniz, Ayce Unverdi The Korean Academy of Prosthodonitics 2017 The Journal of Advanced Prosthodontics Vol.9 No.5

PURPOSE. Bacterial adhesion on provisional crown materials retained for a long time can influence the duration for which permanent prosthetic restorations can be healthily worn in the oral cavity. The aim of this study was to compare seven different commonly used provisional crown materials with regard to Streptococcus mutans and Candida albicans surface adhesion. MATERIALS AND METHODS. For each group, twenty specimens of the provisional fixed prosthodontic materials TemDent ($Sch{\ddot{u}}tz$), Imident (Imicryl), Tab 2000 (Kerr), Structur Premium (Voco), Systemp (Ivoclar Vivadent), Acrytemp (Zhermack), and Takilon-BBF (Takilon) were prepared (diameter, 10.0 mm; height, 2.0 mm). Surface roughness was assessed by atomic force microscopy. Each group was then divided into 2 subgroups (n=10) according to the microbial suspensions used: S. mutans and C. albicans. The specimens were incubated at $37^{\circ}C$ with S. mutans or C. albicans for seven days. Bacterial adherence on surfaces was assessed using the 2,3-bis[2-methyloxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxanilide (XTT) assay. RESULTS. S. mutans showed maximum adhesion to Structur, followed by Systemp, Acrytemp, Takilon, Tab 2000, Imident, and TemDent (P<.05). The highest vital C. albicans adhesion was noted on Takilon, followed by Imident and Tab 2000; the lowest adhesion was noted on Systemp (P<.05). CONCLUSION. The materials showed significant differences in the degree of bacterial adhesion. C. albicans showed higher surface adhesion than S. mutans on provisional crown and fixed partial denture denture materials.

In vitro study of fracture strength of provisional crown materials

Isil Karaokutan,Gulsum Sayin,Ozlem Kara 대한치과보철학회 2015 The Journal of Advanced Prosthodontics Vol.7 No.1

PURPOSE The purpose of this report was to evaluate the effect of the fabrication method and material type on the fracture strength of provisional crowns. MATERIALS AND METHODS A master model with one crown (maxillary left second premolar) was manufactured from Cr-Co alloy. The master model was scanned, and the data set was transferred to a CAD/CAM unit (Yenamak D50, Yenadent Ltd, Istanbul, Turkey) for the Cercon Base group. For the other groups, temporary crowns were produced by direct fabrication methods (Imident, Temdent, Structur Premium, Takilon, Systemp c&b II, and Acrytemp). The specimens were subjected to water storage at 37℃ for 24 hours, and then they were thermocycled (TC, 5000×, 5-55℃) (n=10). The maximum force at fracture (Fmax) was measured in a universal test machine at 1 mm/min. Data was analyzed by non-parametric statistics (α=.05). RESULTS Fmax values varied between 711.09-1392.1 N. In the PMMA groups, Takilon showed the lowest values (711.09 N), and Cercon Base showed the highest values (959.59 N). In the composite groups, Structur Premium showed the highest values (1392.1 N), and Acrytemp showed the lowest values (910.05 N). The composite groups showed significantly higher values than the PMMA groups (P=.01). CONCLUSION Composite-based materials showed significantly higher fracture strengths than PMMA-based materials. The CAD-CAM technique offers more advantages than the direct technique.

Effect of surface treatment and luting agent type on shear bond strength of titanium to ceramic materials

Isil Karaokutan,Gulsum Sayin Ozel 대한치과보철학회 2022 The Journal of Advanced Prosthodontics Vol.14 No.2

PURPOSE. This study aimed to compare the effect of different surface treatments and luting agent types on the shear bond strength of two ceramics to commercially pure titanium (Cp Ti). MATERIALS AND METHODS. A total of 160 Cp Ti specimens were divided into 4 subgroups (n = 40) according to surface treatments received (control, 50 μm airborne-particle abrasion, 110 μm airborne-particle abrasion, and tribochemical coating). The cementation surfaces of titanium and all-ceramic specimens were treated with a universal primer. Two cubic all-ceramic discs (lithium disilicate ceramic (LDC) and zirconia-reinforced lithium silicate ceramic (ZLC)) were cemented to titanium using two types of resin-based luting agents: self-cure and dual-cure (n = 10). After cementation, all specimens were subjected to 5000 cycles of thermal aging. A shear bond strength (SBS) test was conducted, and the failure mode was determined using a scanning electron microscope. Data were analyzed using three-way ANOVA, and the Tukey-HSD test was used for post hoc comparisons (P < .05). RESULTS. Significant differences were found among the groups based on surface treatment, resin-based luting agent, and ceramic type (P < .05). Among the surface treatments, 50 μm air-abrasion showed the highest SBS, while the control group showed the lowest. SBS was higher for dual-cure resin-based luting agent than self-cure luting agent. ZLC showed better SBS values than LDC. CONCLUSION. The cementation of ZLC with dual-cure resin-based luting agent showed better bonding effectiveness to commercially pure titanium treated with 50 μm airborne-particle abrasion.

In vitro study of fracture strength of provisional crown materials

Karaokutan, Isil,Sayin, Gulsum,Kara, Ozlem The Korean Academy of Prosthodonitics 2015 The Journal of Advanced Prosthodontics Vol.7 No.1

PURPOSE. The purpose of this report was to evaluate the effect of the fabrication method and material type on the fracture strength of provisional crowns. MATERIALS AND METHODS. A master model with one crown (maxillary left second premolar) was manufactured from Cr-Co alloy. The master model was scanned, and the data set was transferred to a CAD/CAM unit (Yenamak D50, Yenadent Ltd, Istanbul, Turkey) for the Cercon Base group. For the other groups, temporary crowns were produced by direct fabrication methods (Imident, Temdent, Structur Premium, Takilon, Systemp c&b II, and Acrytemp). The specimens were subjected to water storage at $37^{\circ}C$ for 24 hours, and then they were thermocycled (TC, $5000{\times}$, $5-55^{\circ}C$) (n=10). The maximum force at fracture (Fmax) was measured in a universal test machine at 1 mm/min. Data was analyzed by non-parametric statistics (${\alpha}$=.05). RESULTS. Fmax values varied between 711.09-1392.1 N. In the PMMA groups, Takilon showed the lowest values (711.09 N), and Cercon Base showed the highest values (959.59 N). In the composite groups, Structur Premium showed the highest values (1392.1 N), and Acrytemp showed the lowest values (910.05 N). The composite groups showed significantly higher values than the PMMA groups (P=.01). CONCLUSION. Composite-based materials showed significantly higher fracture strengths than PMMA-based materials. The CADCAM technique offers more advantages than the direct technique.

Association of Secondary Amyloidosis with Common Variable Immune Deficiency and Tuberculosis

Aykut Ferhat Celik,Mehmet Riza Altiparmak,Gulsum Emel Pamuk,Omer Nuri Pamuk,Fehmi Tabak 연세대학교의과대학 2005 Yonsei medical journal Vol.46 No.6

This paper describes the first case of common variable immunodeficiency (CVID) and AA amyloidosis. A recently treated tuberculosis, and chronic inflammation induced by frequent respiratory tract infections, were thought to be responsible for the amyloidosis. No other reason for this condition could be detected. Although T cell dysfunction in some CVID patients has been reported, pulmonary tuberculosis is quite rare with this condition. Bacterial or viral agents or evidence in favour of intestinal tuberculosis, which would explain this patient's recurrent diarrhea, were not found. In this case, the response of the attacks of diarrhea to metranidazole and the histologic observation of extensive intestinal amyloid deposition, which is known to decrease intestinal motility, made us conclude that the diarrhea was associated with bacterial overgrowth. In this report, we discuss the association of CVID and tuberculosis to secondary amyloidosis and recurrent diarrhea.

Knowledge, Attitude and Practices of Technicians Working at Hospitals Towards Testicular Cancer and Self-examination of Testicles in Turkey

Ozturk, Ahmet,Unalan, Demet,Guleser, Gulsum Nihal Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.23

Background: The present study was conducted to determine knowledge level of technicians working in hospitals about testicular cancer (TC) and self-examination of testicles (TSE) and to determine levels of consciousness and implementation status about TSE. Materials and Methods: This cross-sectional study was conducted with technicians working in hospitals (n=243) between $2^{nd}$ January-$31^{st}$ May 2012 at private and governmental hospitals in urban Kayseri. Healthy control subjects (n=235) who were similar to technicians in terms of age, education level and income status were also included to the study. Chi-square test was used in comparison of categorical variables. Results: Technicians were significantly more aware of TC than controls, but the latter were found to have significantly more information about TSE. There was no significant difference between the two groups in terms of knowing how to do TSE and 80% of both groups were unaware this exam. Of technicians, 19.8% and of controls, 25.5% did TSE, the difference being statistically insignificant. First reason for not doing TSE was "not-knowing" among technicians (48.1%) while it was "ignorance" among controls (66.8%). Of technicians doing TSE, 37.5% did as it came to their mind while 51.7% of controls performed TSE several times in the previous year. Technicians were significantly more afraid of getting TC than controls (p=0.037). Conclusions: It was determined in the present study that rates of TSE were similar between technicians and controls. However compliance with the recommended frequency and right method was low. Consequently, public health education should be planned and applied in order to increase the knowledge of TC and TSE.

Effect of Maternal Depression on Brain-derived Neurotrophic Factor Levels in Fetal Cord Blood

Erdem Onder Sonmez,Faruk Uguz,Mine Sahingoz,Gulsum Sonmez,Nazmiye Kaya,Mehmet Akif Camkurt,Zeynel Gokmen,Mustafa Basaran,Kazim Gezginc,Sami Sait Erdem,Hasan Haluk Dulger,Erkan Tasyurek 대한정신약물학회 2019 CLINICAL PSYCHOPHARMACOLOGY AND NEUROSCIENCE Vol.17 No.2

Objective: We aimed to assess the association between cord blood brain-derived neurotrophic factor (BDNF) concentration and maternal depression during pregnancy. Methods: A total of 48 pregnant women, admitted for elective caesarean section to Department of Obstetrics and Gynecology, The Konya Research and Training Hospital and Konya Necmettin Erbakan University Meram Medical Faculty, were included in this study. The study group included 23 women diagnosed as having depression during pregnancy and the control group included 25 pregnant women who did not experience depression during pregnancy. Results: The groups had similar sociodemographic characteristics. Cord blood BDNF concentration was significantly lower in babies born to mothers with major depression as compared with those in the control group. We didn’t find any correlation between the umbilical cord blood BDNF levels and BDI scores. Conclusion: The results suggest that the existence of major depression in pregnant women may negatively affect fetal circulating BDNF levels.

Ultrasonographic and macroscopic comparison of the thickness of the capsule, corium, and soft tissues in bovine claws: an in vitro study

Goksen Cecen,Hakan Salci,Deniz Seyrek Intas,Nureddin Celimli,Gulsum Ulke Caliskan 대한수의학회 2015 Journal of Veterinary Science Vol.16 No.1

This study aimed to compare thickness of the capsule, corium, and soft tissues measured ultrasonographically and macroscopically in selectedregions of bovine claws. A hundred and twenty claws (n = 120) of 15 healthy Holstein bovines were obtained. After cleaning the claws,ultrasonographic measurement of the capsule, corium, and soft tissues was performed while submerging the claws in a water bath. Macroscopicmeasurements were taken after cutting of the claws axially. These values were compared statistically. According to the macroscopicmeasurements, the mean thickness ± standard deviation (SD) of the capsule for dorsal wall and sole was 6.2 ± 0.1 and 9.5 ± 0.4 mm, respectively. The thickness of the corium and soft tissues for dorsal wall and sole was 4.5 ± 0.1 and 5.3 ± 0.1 mm, respectively. Ultrasonographically, themean thickness ± SD of the capsule for dorsal wall and sole was 4.7 ± 0.1 and 7.8 ± 0.3 mm, respectively. The thickness of the corium andsoft tissues for dorsal wall and sole was 4.3 ± 0.1 and 5.9 ± 0.2 mm, respectively. Findings demonstrated that ultrasonography can be reliablyto measure of the thickness of the hoof capsule, corium, and soft tissue in bovine claw.