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( Won Cheol Kim ),( Jung Joon Lee ),( Sang Gi Paik ),( Young Soo Hong ) 한국미생물 · 생명공학회 2010 Journal of microbiology and biotechnology Vol.20 No.11
In the Streptomyces hygroscopicus JCM4427 geldanamycin biosynthetic gene cluster, five putative regulatory genes were identified by protein homology searching. Among those genes, gel14, gel17, and gel19 are located downstream of polyketide synthase genes. Gel14 and Gel17 are members of the LAL family of transcriptional regulators, including an ATP/GTP-binding domain at the N-terminus and a DNA-binding helix-turn-helix domain at the Cterminus. Gel19 is a member of the TetR family of transcriptional regulators, which generally act to repress transcription. To verify the biological significance of the putative regulators in geldanamycin production, they were individually characterized by gene disruption, genetic complementation, and transcriptional analyses. All three genes were confirmed as positive regulators of geldanamycin production. Specifically, Gel17 and Gel19 are required for gel14 as well as gelA gene expression.
Optimized Lamina Size Maximizing Yield for Cross Laminated Timber Using Domestic Trees
Gi Young Jeong,Jun Jae Lee,Hwanm Yeong Yeo,Jung Pyo Hong,Hyung Kun Kim,Won Tek So,Woo Yang Chung 한국목재공학회 2013 목재공학 Vol.41 No.2
The goal of this study was to find the optimum lamina size from red pine, (Pinus densiflora), and Japanese cedar, (Cryptomeria japonica), logs for the cross laminated timber, (CLT), production. From visual inspection of the logs from two species, red pine log showed a larger knot and warp compared to the Japanese cedar Different cross-sectional size of lamina, (110 mm × 30 mm, 110mm × 40 mm, 110 mm × 50 mm, 50 mm × 30 mm, 30 mm × 30 mm), from two species were analyzed for yield and grade. Regardless of the species, the optimized cross sectional size for maximizing the yield was 110 mm × 30 mm. In grading for the different size laminas from Japanese cedar and red pine, a higher percentage of the first and second grade was found from the 110 mm × mm lamina cut.
The Antioxidant Hesperetin Protects Porcine Oocyte from Damage by In Vitro Aging
Won-Jae Kim,Seung-Eun Lee,Yun-Gwi Park,Sang-Gi Jeong,Jae-Wook Yoon,Chan-Oh Park,Hyo-Jin Park,Dong-Yong Hong,Eun-Young Kim,Se-Pill Park 한국동물생명공학회(구 한국동물번식학회) 2018 발생공학 국제심포지엄 및 학술대회 Vol.2018 No.06
Hesperetin (H), one of the citrus flavonoids, shows a various of pharmacological properties including antioxidant, anti‐inflammatory, and anticancer. The purpose of this study is to prevent in vitro aging by treating hesperetin to protect it from oxidative stress. Porcine oocytes were matured in vitro for 44 h (control) and for an additional 24 h in the presence of 0, 0.1, 1, 10, and 100 μM hesperetin (aging, H-1, H-10, H-100 and H-250, respectively). The study investigated the effect of proper concentration of hesperetin on nuclear maturation rate, and ROS level, apoptosis proportion, and the developmental capacity of aging porcine oocytes. The H-100 group was the most effective of protect for oxidate stress. The all results of the investigated showed that the aging group was significantly worse than the control group. During in vitro aging, normal spindle formation, apoptotic proportion, and expression of maturation marker genes were protected by the H-100 group, that result then the H-100 group was similar to the control group. The ROS level was the H-100 group better than the aging group. The estrogen receptor gene was examined to determine how hesperetin enters the porcine oocytes. As a result, it was confirmed that estrogen receptor was used because the H-100 group was expressed more than the aging group. Thus, the hesperetin is effective agent to protect from the oxidative stress during in vitro aging of porcine oocytes. Therefore, hesperetin will improve the quality of mature oocytes by allowing in vitro experiments to be protected from oxidative stress.
Gi Ja Lee,Seok Keun Choi,Yun Hye Eo,Sung Wook Kang,Samjin Choi,Jeong Hoon Park,Ji Eun Lim,Kyung Won Hong,Hyun Seok Jin,Berm Seok Oh,Hun Kuk Park 대한생리학회-대한약리학회 2009 The Korean Journal of Physiology & Pharmacology Vol.13 No.1
During operations, neurosurgeons usually perform multiple temporary occlusions of parental artery, possibly resulting in the neuronal damage. It is generally thought that neuronal damage by cerebral ischemia is associated with extracellular concentrations of the excitatory amino acids. In this study, we measured the dynamics of extracellular glutamate release in 11 vessel occlusion (VO) model to compare between single occlusion and repeated transient occlusions within short interval. Changes in cerebral blood flow were monitored by laser-Doppler flowmetry simultaneously with cortical glutamate level measured by amperometric biosensor. From real time monitoring of glutamate release in 11 VO model, the change of extracellular glutamate level in repeated transient occlusion group was smaller than that of single occlusion group, and the onset time of glutamate release in the second ischemic episode of repeated occlusion group was delayed compared to the first ischemic episode which was similar to that of single 10 min ischemic episode. These results suggested that repeated transient occlusion induces less glutamate release from neuronal cell than single occlusion, and the delayed onset time of glutamate release is attributed to endogeneous protective mechanism of ischemic tolerance.
Magnetism and Magnetocrystalline Anisotropy at fcc Fe (001) Surface
Won Seok Yun,Gi-Beom Cha,Soon Cheol Hong 한국자기학회 2008 Journal of Magnetics Vol.13 No.4
The size and surface effects on the magnetism of a fcc Fe (001) surface was investigated by performing firstprinciples calculations on 3, 5, 7, and 9 monolayers fcc Fe (001) single slabs with two different two-dimensional lattice constants, a = 3.44 A (System Ⅰ) and 3.65 A (System Ⅱ), using the all-electron full-potential linearized augmented plane wave method within a generalized gradient approximation. The surface layers were coupled ferromagnetically to the subsurface layer in both systems. However, the magnetism of the inner layers was quite different from each other. While all the inner layers of System Ⅱ were ferromagnetically coupled in the same way as the surface layer, the inner layers of System I showed a peculiar magnetism, bilayer antiferromagnetism. The calculated spin magnetic moments per Fe atom were approximately 2.7 and 2.9 μB at the surface for Systems Ⅰ and Ⅱ, respectively, due to the almost occupied Fe d-state being in the majority spin state and band narrowing. The spin orientations of System I were out-of-plane regardless of its thickness, whereas the orientation of System Ⅱ changed from out-of-plane to in-plane with increasing thickness.