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Xu Weimin,Tang Wenbo,Ding Wenjun,Hua Zhebin,Wang Yaosheng,Ge Xiaolong,Cui Long,Wu Xiaojian,Zhou Wei,Ding Zhao,Du Peng 거트앤리버 소화기연관학회협의회 2024 Gut and Liver Vol.18 No.1
Background/Aims: Total proctocolectomy with ileal pouch-anal anastomosis (IPAA) is widely accepted as a radical surgery for refractory ulcerative colitis (UC). Definite results on the appropriate pouch length for an evaluation of the risk-to-benefit ratio regarding technical complications and long-term quality of life (QOL) are still scarce. Methods: Data on UC patients who underwent IPAA from 2008 to 2022 in four well-established pouch centers affiliated to China UC Pouch Center Union were collected. Results: A total of 208 patients with a median follow-up time of 6.0 years (interquartile range, 2.3 to 9.0 years) were enrolled. The median lengths of the patients’ short and long pouches were 14.0 cm (interquartile range, 14.0 to 15.0 cm) and 22.0 cm (interquartile range, 20.0 to 24.0 cm), respectively. Patients with a short J pouch configuration were less likely to achieve significantly improved long-term QOL (p=0.015) and were prone to develop late postoperative complications (p=0.042), such as increased defecation frequency (p=0.003) and pouchitis (p=0.035). A short ileal pouch was an independent risk factor for the development of late postoperative complications (odds ratio, 3.100; 95% confidence interval, 1.519 to 6.329; p=0.002) and impaired long-term QOL improvement (odds ratio, 2.221; 95% confidence interval, 1.218 to 4.050, p=0.009). Conclusions: The length of the J pouch was associated with the improvement in long-term QOL and the development of late post-IPAA complications. A long J pouch configuration could be a considerable surgical option for pouch construction.
Long Yuan,Li-xin Lu,Ya-li Tang,Chang-feng Ge 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.2
Application of high-hydrostatic-pressure (HHP) sterilization technology to boiled bamboo shoots was preliminarily discussed. An improved Weibull model that considered pressure and time as independent variables simultaneously for simulating the sterilization result was obtained by fitting the microbial lethal curves with high precision (R2>0.98). HHP sterilization parameters could be calculated using this model with a certain disinfection rate. For a sterilization rate of 99.5%, treatment with a pressure of 400 MPa for 6 min or 500 MPa for 3 min at room temperature could exterminate all pathogenic microorganisms in boiled bamboo shoots, and control the total number of colonies at a low level below 100 CFU/g. Comparisons of the effects of HHP, high-pressure steam and microwave on the softness and contents of soluble protein and vitamin C of samples were also made. The results showed that the extent of damage of the products’ original quality caused by HHP sterilization was less than that caused by the thermal and microwave treatment processes with equivalent sterilization percentages.
Long, Jin,Zhang, Zhong-Bo,Liu, Zhe,Xu, Yuan-Hong,Ge, Chun-Lin Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.6
Background: Loss of heterozygosity (LOH) on chromosomal regions is crucial in tumor progression and this study aimed to identify genome-wide LOH in pancreatic cancer. Materials and Methods: Single-nucleotide polymorphism (SNP) profiling data GSE32682 of human pancreatic samples snap-frozen during surgery were downloaded from Gene Expression Omnibus database. Genotype console software was used to perform data processing. Candidate genes with LOH were screened based on the genotype calls, SNP loci of LOH and dbSNP database. Gene annotation was performed to identify the functions of candidate genes using NCBI (the National Center for Biotechnology Information) database, followed by Gene Ontology, INTERPRO, PFAM and SMART annotation and UCSC Genome Browser track to the unannotated genes using DAVID (the Database for Annotation, Visualization and Integration Discovery). Results: The candidate genes with LOH identified in this study were MCU, MICU1 and OIT3 on chromosome 10. MCU was found to encode a calcium transporter and MICU1 could encode an essential regulator of mitochondrial $Ca^{2+}$ uptake. OIT3 possibly correlated with calcium binding revealed by the annotation analyses and was regulated by a large number of transcription factors including STAT, SOX9, CREB, NF-kB, PPARG and p53. Conclusions: Global genomic analysis of SNPs identified MICU1, MCU and OIT3 with LOH on chromosome 10, implying involvement of these genes in progression of pancreatic cancer.
Long, Zhi,Gao, Liqin,Li, Yankai,Kang, Baotao,Lee, Jin Yong,Ge, Junjie,Liu, Changpeng,Ma, Shuhua,Jin, Zhao,Ai, Hongqi American Chemical Society 2017 ACS APPLIED MATERIALS & INTERFACES Vol.9 No.44
<P>The self-assembly powder (SAP) with varying Nafion content was synthesized and characterized by XRD, XPS, HRTEM, and mapping. It is observed that the oxygen from oxygen functional groups transfers to the surface of Pt and generate PtO during the process of self-assembly with the mechanism of micro galvanic cell, where Pt, carbon black, and Nafion act as the anode, cathode and electrolyte, respectively. The appearance of PtO on the surface of Pt leads to a turnover of Nafion structure, and therefore more hydrophilic sulfonic groups directly contact with Pt, and thus the triple-phase boundary (TPB) has been expanded.</P>
Jianhua Ge,Jing Xu,Yujie Liu,Long Zhang,Lan Wang,Dehan Wu 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2019 NANO Vol.14 No.11
Nitrogen photo fixation mediated by various photocatalysts has received extensive concerns in recent years. In this work, a general strategy is proposed to enhance nitrogen photo fixation performance with some W18O49-based photocatalysts under visible-light irradiation. A series of photocatalysts were successfully synthesized in the presence of some noble-metal-free transition metals (Fe, Co and Ni) to modify the urchin-like W18O49. The resulting photocatalysts were systematically characterized using XRD, SEM, EDS, XPS, PL, N2 adsorption–desorption isotherms, and DRS. Structural characterization of the photocatalysts revealed that the crystal morphology features were preserved, optional harvesting properties were enhanced, BET specific surface area was extended, and migration efficiency of the photo-induced charge carriers was significantly improved in the modified-W18O49 materials. Therefore, a progressed nitrogen photo fixation performance was observed. The maximum production of NH4 + was approximately reached to 2752 μg · g -1 catal. under the optimum reaction conditions. Furthermore, a plausible nitrogen photo fixation mechanism in the presence of the synthesized composites is also proposed.
An Improved ET-GM-PHD Filter for Multiple Closely-Spaced Extended Target Tracking
Jin-Long Yang,Peng Li,Le Yang,Hongwei Ge 제어·로봇·시스템학회 2017 International Journal of Control, Automation, and Vol.15 No.1
This paper presents an enhanced version of the ET-GM-PHD algorithm, a recently developed multipleextended target tracking (METT) technique. The original ET-GM-PHD filter tends to underestimate the targetnumber, because the likelihood estimate in the state update process may poorly approximate the real one whentargets are close to each other. The proposed algorithm addresses this drawback via introducing a new penaltystrategy in estimating the measurement likelihood. Besides, Gaussian component labeling technique is adopted toobtain individual target tracks. Simulations show that for closely-spaced extended target tracking, the improvedmethod achieves track continuity and exhibits better estimation accuracy over the original ET-GM-PHD filter.
Jin-Long Ren,Xiongbing Tu,Jing Ge,Li Zhao,Zehua Zhang 한국응용곤충학회 2016 Journal of Asia-Pacific Entomology Vol.19 No.1
Calliptamus italicus is a migratory species of grasshopper that can cause serious damage to grasslands in Xinjiang, China. In this study,we examined the effects of different temperatures (23, 26, 29, 32, and 35 °C) on the lifecycle and life table parameters. The results showed that temperature strongly influences the growth and development of C. italicus. At temperatures ranging from 23 to 35 °C, the development time was negatively linearly related to temperature (P b 0.01). The time of development from an egg to an adult (pre-oviposition) ranged from86.8 d at 26 °C to 40.6 d at 35 °C, and no oviposition occurred at 23 °C. An average of 687.1 degree-days was required for complete development to occur above the lower threshold temperature (18.3 °C). Egg hatching rates and nymph and pre-adult survival rates were lowest at 23 °C (95.6%, 72.6%, and 0%, respectively) and highest at 32 °C (97.8%, 86.2%, and 88.7%, respectively). The mean total fecundity ranged from 41 eggs/female at 26 °C to 55 eggs/female at 32 °C. The sex ratio (% females) ranged from30 to 52%. The intrinsic rate of population increase (rm) at the different temperatures ranged from 0.0020 to 0.0447 d−1, with the highest value recorded at 32 °C. These results indicate that temperature significantly affects the biology of C. italicus and that the optimal temperature range for its development is 26–32 °C.
Qing Ge,Jian-wei Mao,An-qiang Zhang,Yong-jiang Wang,Pei-long Sun 한국식품과학회 2013 Food Science and Biotechnology Vol.22 No.2
A purified water-soluble polysaccharide was isolated from the fruiting bodies of sanghuang mushroom (Phellinus baumii Pilát) using hot water extraction, DEAE Sepharose Fast Flow anion exchange and High-Resolution Sephacryl S-1000 gel-filtration chromatography. The purified polysaccharide was a complex β-D-glucan, with a molecular mass of 230 kDa. Fourier-transform infrared (FT-IR), methylation analysis, and NMR spectroscopy of sanghuang mushroom polysaccharide (PBF3) indicated that the polysaccharide contained (1→3)-β-D-, (1→4)-β-D-,and branched (1→3,6)-β-D-glucopyranosyl residues. On the basis of hydroxyl radical assay, superoxide radical assay, and DPPH radical assay, its antioxidant activities were investigated. PBF3 had significant effect on scavenging hydroxyl radicals, an equivalent inhibiting ability to vitamin C on superoxide radical, and a little lower scavenging activity on DPPH radical than vitamin C, and should be explored as a novel potential antioxidant.
( Xiang Yang Ge ),( Yan Xu ),( Xiang Chen ),( Long Yun Zhang ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.1
This study describes a novel strategy to regulate the metabolic flux for lactic acid production in Lactobacillus casei. The ldhL gene encoding L-lactate dehydrogenase (L-LDH) was overexpressed in L. casei, and a two-stage oxygen supply strategy (TOS) that maintained a medium oxygen supply level during the early fermentation phase, and a low oxygen supply level in the later phase was carried out. As a consequence, a maximum L-LDH activity of 95.6 U/ml was obtained in the recombinant strain, which was over 4-fold higher than that of the initial strain. Under the TOS for L. casei (pMG-ldhL), the maximum lactic acid concentration of 159.6 g/l was obtained in 36 h, corresponding to a 62.8% increase. The results presented here provide a novel way to regulate the metabolic flux of L. casei for lactic acid production in different fermentation stages, which is available to enhance organic acid production in other strains.