Tracheal Membrane Rupture as the Cause of Pneumomediastinum in a Patient with COVID-19

Fu Bao,Hu Jie,Chen Tao,Fu Xiaoyun 대한영상의학회 2022 Korean Journal of Radiology Vol.23 No.4

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Spectroscopic Determination of the Species Fractions and the Power Profiles of the Diagnostic Neutral Beam on the HT-7 Tokamak

Jia Fu,Yuejiang Shi,Yingying Li,Fudi Wang,Sheng Liu,Jian Zhang,Jun Li,Yiyyun Huang,Yuanlai Xie,Zhimin Liu,Chundong Hu,Chundong Hu,DNB Team,William Rowan,He Huang 한국물리학회 2011 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.58 No.5

A diagnostic neutral beam (DNB) has been installed on the HT-7 tokamak for the measurement of charge exchange recombination spectroscopy (CXRS). The H_α-light Doppler shift spectroscopy from the drifted duct is measured to determine the components of the neutral beam. The fractions of neutral beam species are investigated under a wide range of arc voltages and extraction high voltages of the beam aimed to the optimized species fractions for the CXRS applications. A magnet ring is used to improve the magnetic property of the ion source. The result shows that the full-energy component fractions increase from 19 to 25 percent with optimization of the beam operation, but with a dramatic increase of the water component. There are nine optical fiber channels observing one section of the beam simultaneously for this spectroscopy, which provides information of the power profiles of the beam. The full width at half maximum (FWHM) of the beam profile is 8 cm, as measured by using the spectroscopy.

Proteomic Profiles of Mouse Neuro N2a Cells Infected with Variant Virulence of Rabies Viruses

( Wang Xiao Hu ),( Shou Feng Zhang ),( Cheng Long Sun ),( Zi Guo Yuan ),( Xian Fu Wu ),( Dong Xia Wang ),( Zhuang Ding ),( Rong Liang Hu ) 한국미생물 · 생명공학회 2011 Journal of microbiology and biotechnology Vol.21 No.4

We characterized the proteomes of murine N2a cells following infection with three rabies virus (RV) strains, characterized by distinct virulence phenotypes (i.e., virulent BD06, fixed CVS-11, and attenuated SRV9 strains), and identified 35 changes to protein expression using twodimensional gel electrophoresis in whole-cell lysates. The annotated functions of these proteins are involved in various cytoskeletal, signal transduction, stress response, and metabolic processes. Specifically, a-enolase, prx-4, vimentin, cytokine-induced apoptosis inhibitor 1 (CIAPIN1) and prx-6 were significantly up-regulated, whereas Trx like-1 and galectin-1 were down-regulated following infection of N2a cells with all three rabies virus strains. However, comparing expressions of all 35 proteins affected between BD06-, CVS-11-, and SRV9-infected cells, specific changes in expression were also observed. The up-regulation of vimentin, CIAPIN1, prx-4, and 14-3-3 θ/δ, and downregulation of NDPK-B and HSP-1 with CVS and SRV9 infection were ≥2 times greater than with BD06. Meanwhile, Zfp12 protein, splicing factor, and arginine/serine-rich 1 were unaltered in the cells infected with BD06 and CVS- 11, but were up-regulated in the group infected with SRV9. The proteomic alterations described here may suggest that these changes to protein expression correlate with the rabies virus`` adaptability and virulence in N2a cells, and hence provides new clues as to the response of N2a host cells to rabies virus infections, and may also aid in uncovering new pathways in these cells that are involved in rabies infections. Further characterization of the functions of the affected proteins may contribute to our understanding of the mechanisms of RV infection and pathogenesis.

Hydrogen sulfide inhibits the growth of Escherichia coli through oxidative damage

Liu-Hui Fu,Zeng-Zheng Wei,Kang-Di Hu,Lan-Ying Hu,Yan-Hong Li,Xiao-Yan Chen,Zhuo Han,Gai-Fang Yao,Hua Zhang 한국미생물학회 2018 The journal of microbiology Vol.56 No.4

Many studies have shown that hydrogen sulfide (H2S) is both detrimental and beneficial to animals and plants, whereas its effect on bacteria is not fully understood. Here, we report that H2S, released by sodium hydrosulfide (NaHS), significantly inhibits the growth of Escherichia coli in a dose-dependent manner. Further studies have shown that H2S treatment stimulates the production of reactive oxygen species (ROS) and decreases glutathione (GSH) levels in E. coli, resulting in lipid peroxidation and DNA damage. H2S also inhibits the antioxidative enzyme activities of superoxide dismutase (SOD), catalase (CAT) and glutathione reductase (GR) and induces the response of the SoxRS and OxyR regulons in E. coli. Moreover, pretreatment with the antioxidant ascorbic acid (AsA) could effectively prevent H2S-induced toxicity in E. coli. Taken together, our results indicate that H2S exhibits an antibacterial effect on E. coli through oxidative damage and suggest a possible application for H2S in water and food processing.

A Piperidine Alkaloid and Limonoids from Arisaema decipiens, a Traditional Antitumor Herb Used by the Dong People

Fu-Wei Zhao,Min Luo,Yue-Hu Wang,Ma-Lin Li,Gui-Hua Tang,Chun-Lin Long 대한약학회 2010 Archives of Pharmacal Research Vol.33 No.11

A new piperidine alkaloid and three known tetranortriterpenoids were isolated from the methanol extracts of the rhizomes of Arisaema decipiens Schott (Araceae) and their chemical structures were identified as (-)-(2R*,3S*,6S*)-N,2-dimethyl-3-hydroxy-6-(9-phenylnonyl) piperidine (1), 6-deacetylnimbin (2), 28-deoxonimbolide (3) and nimbin (4). The N-methylated derivative (1a) of 1 was synthesized. Compound 1 exhibited weak inhibitory activity against the MCF-7 cell line, while compound 1a showed potential inhibitory activity against the MCF-7 cell line with an IC50 value of 4.6 μM and weak inhibitory activity against K562 and SK-OV-3 cells. This plant in genus Arisaema is firstly reported as the source of limonoids that are considered a natural antitumor herbal medicine.

Stability Control of Energy Storage Voltage Source Inverters in Isolated Power Systems

Hu, Jian,Fu, Lijun The Korean Institute of Power Electronics 2018 JOURNAL OF POWER ELECTRONICS Vol.18 No.6

Isolated power systems (IPS) are often characterized by a weak grid due to small power grids. The grid side voltage is no longer equivalent to an ideal voltage source of an infinitely big power grid. The conversion control of new energy sources, parameter perturbations as well as the load itself can easily cause the system voltage to oscillate or to become unstable. To solve this problem, increasing the energy-storage power sources is usually used to improve the reliability of a system. In order to provide support for the voltage, the energy-storage power source inverter needs an method to control the voltage source. Therefore, this paper has proposed the active damping control of a voltage source inverter (VSI) based on virtual compensation. By simplifying the VSI double closed-loop control, two feedback compensation channels have been constructed to reduce the VSI output impedance without changing the characteristics of the voltage gain of a system. This improvement allows systems to operate stably in a larger range. A frequency-domain analysis, and simulation and experimental results demonstrate the feasibility and effectiveness of the proposed method.

Effects of Gastric Cancer Cells on the Differentiation of Treg Cells

Hu, Jing-Lan,Yang, Zhen,Tang, Jian-Rong,Fu, Xue-Qin,Yao, Lan-Jie Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.8

The aim of this study was evaluated the prevalence of Treg cells in peripheral blood in patients with gastric cancer, and investigate the effect of gastric cancer cells on their differentiation. ELISA was employed to assess the concentrations of TGF-${\beta}$ and IL-10 in gastric cancer patients' serum. Then, mouse gastric cancer cells were co-cultured with T lymphocytes or T lymphocytes + anti-TGF-${\beta}$. Flow cytometric analysis and RT-PCR were then performed to detect Treg cells and TGF-${\beta}$ and IL-10 expression in gastric cancer cells. Our data showed that the expression of TGF-${\beta}$ and IL-10 in the patients with gastric cancer was increased compared to the case with healthy donors. The population of Treg cells and the expression levels of TGF-${\beta}$ and IL-10 in the co-culture group were much higher than in the control group (18.6% vs 9.5%) (P<0.05). Moreover, the population of Treg cells and the expression levels of TGF-${\beta}$ and IL-10 in the co-culture systerm were clearly decreased after addition of anti-TGF-${\beta}$ (7.7% vs 19.6%) (P<0.01). In conclusion, gastric cancer cells may induce Treg cell differentiation through TGF-${\beta}$, and further promote immunosuppression.

Suppression of Ku80 Correlates with Radiosensitivity and Telomere Shortening in the U2OS Telomerase-negative Osteosarcoma Cell Line

Hu, Liu,Wu, Qin-Qin,Wang, Wen-Bo,Jiang, Huan-Gang,Yang, Lei,Liu, Yu,Yu, Hai-Jun,Xie, Cong-Hua,Zhou, Yun-Feng,Zhou, Fu-Xiang Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.2

Ku70/80 heterodimer is a central element in the nonhomologous end joining (NHEJ) DNA repair pathway, Ku80 playing a key role in regulating the multiple functions of Ku proteins. It has been found that the Ku80 protein located at telomeres is a major contributor to radiosensitivity in some telomerase positive human cancer cells. However, in ALT human osteosarcoma cells, the precise function in radiosensitivity and telomere maintenance is still unknown. The aim of this study was to investigate the effects of Ku80 depletion in the U2OS ALT cell line cell line. Suppression of Ku80 expression was performed using a vector-based shRNA and stable Ku80 knockdown in cells was verified by Western blotting. U2OS cells treated with shRNA-Ku80 showed lower radiobiological parameters (D0, Dq and SF2) in clonogenic assays. Furthermore, shRNA-Ku80 vector transfected cells displayed shortening of the telomere length and showed less expression of TRF2 protein. These results demonstrated that down-regulation of Ku80 can sensitize ALT cells U2OS to radiation, and this radiosensitization is related to telomere length shortening.

MOMENT CONVERGENCE RATES OF LIL FOR NEGATIVELY ASSOCIATED SEQUENCES

Fu, Ke-Ang,Hu, Li-Hua Korean Mathematical Society 2010 대한수학회지 Vol.47 No.2

Let {$X_n;n\;\geq\;1$} be a strictly stationary sequence of negatively associated random variables with mean zero and finite variance. Set $S_n\;=\;{\sum}^n_{k=1}X_k$, $M_n\;=\;max_{k{\leq}n}|S_k|$, $n\;{\geq}\;1$. Suppose $\sigma^2\;=\;EX^2_1+2{\sum}^\infty_{k=2}EX_1X_k$ (0 < $\sigma$ < $\infty$). We prove that for any b > -1/2, if $E|X|^{2+\delta}$(0<$\delta$$\leq$1), then $$lim\limits_{\varepsilon\searrow0}\varepsilon^{2b+1}\sum^{\infty}_{n=1}\frac{(loglogn)^{b-1/2}}{n^{3/2}logn}E\{M_n-\sigma\varepsilon\sqrt{2nloglogn}\}_+=\frac{2^{-1/2-b}{\sigma}E|N|^{2(b+1)}}{(b+1)(2b+1)}\sum^{\infty}_{k=0}\frac{(-1)^k}{(2k+1)^{2(b+1)}}$$ and for any b > -1/2, $$lim\limits_{\varepsilon\nearrow\infty}\varepsilon^{-2(b+1)}\sum^{\infty}_{n=1}\frac{(loglogn)^b}{n^{3/2}logn}E\{\sigma\varepsilon\sqrt{\frac{\pi^2n}{8loglogn}}-M_n\}_+=\frac{\Gamma(b+1/2)}{\sqrt{2}(b+1)}\sum^{\infty}_{k=0}\frac{(-1)^k}{(2k+1)^{2b+2'}}$$, where $\Gamma(\cdot)$ is the Gamma function and N stands for the standard normal random variable.

Serum IL-33 as a Diagnostic and Prognostic Marker in Non-small Cell Lung Cancer

Hu, Liang-An,Fu, Yu,Zhang, Dan-Ni,Zhang, Jie Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.4

Background: Interleukin-33 (IL-33) has recently been implicated in tumor immunity. The aim of this study was to explore the clinical role of serum IL-33 in patients with non-small-cell lung cancer (NSCLC). Methods: Sera collected from 250 healthy volunteers (HV), 256 patients with benign lung diseases (BLD) and 262 NSCLC cases were subjected to IL-33 ELISA and relationships between serum IL-33 and clinical characteristics were evaluated. Results: Circulating IL-33 levels were higher in the NSCLC group in comparison with the HV and BLD groups (p<0.001). Using a cut-off level 68 pg/ml (95% specificity in the HV group), IL-33 showed a good diagnostic performance for NSCLC. Multivariate survival analysis indicated that serum IL-33 was an independent prognostic factor in the entire NSCLC group [hazards ratio (HR) = 0.64 for low versus high IL-33 levels, 95% confidence interval (CI) 0.50-0.82; p<0.001] and in 165 selected patients with locally advanced or metastatic disease receiving chemoradiotherapy or chemotherapy (HR 0.70, 95% CI 0.52-0.94; p=0.013). Conclusions: IL-33 is a promising potential diagnostic and prognostic marker in NSCLC, independent of the therapeutic intervention.