Comprehensive Evaluation of Impacts of Connecting Distributed Generation to the Distribution Network

Wei Jin,Xuemei Shi,Fei Ge,Wei Zhang,Hongbin Wu,Chengyuan Zhong 대한전기학회 2017 Journal of Electrical Engineering & Technology Vol.12 No.2

In this paper, we study the various impacts of connecting distributed generation (DG) to the distribution network. The comprehensive evaluation index system (CEIS) of four hierarchies is established, considering economy, reliability and voltage quality, and the calculation methods of different indexes are presented. This paper puts forward an improved triangular fuzzy number analytic hierarchy process (ITFNAHP) to weight the second level indexes (SLI) and the third level indexes (TLI), and calculates the variation coefficient to weight the fourth level indexes (FLI). We calculate the comprehensive weight coefficients based on the weight coefficients of the SLI, TLI and FLI, and then calculate the comprehensive evaluation of satisfaction (CES) of different access schemes. On the basis of the IEEE 33-bus example system, simulations of the calculation methods and the comprehensive evaluation method are carried out under different DG access schemes according to the same total investment cost and the same permeability, respectively, and the simulation results are analyzed and discussed.

Cloning and Characterization of Ginsenoside-Hydrolyzing β-Glucosidase from Lactobacillus brevis That Transforms Ginsenosides Rb1 and F2 into Ginsenoside Rd and Compound K

( Fei-liang Zhong ),( Rui Ma ),( Mingliang Jiang ),( Wei-wei Dong ),( Jun Jiang ),( Songquan Wu ),( Donghao Li ),( Lin-hu Quan ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.10

The ginsenoside-hydrolyzing β-glucosidase gene (bgy2) was cloned from Lactobacillus brevis. We expressed this gene in Escherichia coli BL21(DE3), isolated the resulting protein, and then utilized the enzyme for the biotransformation of ginsenosides. The bgy2 gene contains 2,223 bp, and encodes a protein of 741 amino acids that is a member of glycosyl hydrolase family 3. β-Glucosidase (Bgy2) cleaved the outer glucose moieties of ginsenosides at the C-20 position, and the inner glucose at the C-3 position. Under optimal conditions (pH 7.0, 30˚C), we used 0.1 mg/ml Bgy2 in 20 mM sodium phosphate buffer (PBS) for enzymatic studies. In these conditions, 1.0 mg/ml ginsenoside Rb1 and ginsenoside F2 were converted into 0.59 mg/ml ginsenoside Rd and 0.72mg/ml compound K, with molar conversion productivities of 69% and 91%, respectively. In pharmaceutical and commercial industries, this recombinant Bgy2 would be suitable for producting ginsenoside Rd and compound K.

TRIM22 promotes the proliferation of glioblastoma cells by activating MAPK signaling and accelerating the degradation of Raf-1

Fei Xiaowei,Dou Ya-nan,Sun Kai,Wei Jialiang,Guo Qingdong,Wang Li,Wu Xiuquan,Lv Weihao,Jiang Xiaofan,Fei Zhou 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-

The tripartite motif (TRIM) 22 and mitogen-activated protein kinase (MAPK) signaling pathways play critical roles in the growth of glioblastoma (GBM). However, the molecular mechanism underlying the relationship between TRIM22 and MAPK signaling remains unclear. Here, we found that TRIM22 binds to exon 2 of the sphingosine kinase 2 (SPHK2) gene. An ERK1/2-driven luciferase reporter construct identified TRIM22 as a potential activator of MAPK signaling. Knockout and overexpression of TRIM22 regulate the inhibition and activation of MAPK signaling through the RING-finger domain. TRIM22 binds to Raf-1, a negative regulator of MAPK signaling, and accelerates its degradation by inducing K48-linked ubiquitination, which is related to the CC and SPRY domains of TRIM22 and the C1D domain of Raf-1. In vitro and in vivo, an SPHK2 inhibitor (K145), an ERK1/2 inhibitor (selumetinib), and the nonphosphorylated mutant Raf-1S338A inhibited GBM growth. In addition, deletion of the RING domain and the nuclear localization sequence of TRIM22 significantly inhibited TRIM22-induced proliferation of GBM cells in vivo and in vitro. In conclusion, our study showed that TRIM22 regulates SPHK2 transcription and activates MAPK signaling through posttranslational modification of two critical regulators of MAPK signaling in GBM cells.

Biotransformation of Panax ginseng extract by rat intestinal microflora

Wei-Wei Dong,Jinhua Zhao,Fei-Liang Zhong,Wen-Jing Zhu,Jun Jiang,Songquan Wu,Deok-Chun Yang,Donghao Li,Lin-Hu Quan 고려인삼학회 2017 Journal of Ginseng Research Vol.41 No.4

Background: In general, after Panax ginseng is administered orally, intestinal microbes play a crucial role in its degradation and metabolization process. Studies on the metabolism of P. ginseng by microflora are important for obtaining a better understanding of their biological effects. Methods: In vitro biotransformation of P. ginseng extract by rat intestinal microflora was investigated at 37C for 24 h, and the simultaneous determination of the metabolites and metabolic profile of P. ginseng saponins by rat intestinal microflora was achieved using LCeMS/MS. Results: A total of seven ginsenosides were detected in the P. ginseng extract, including ginsenosides Rg1, Re, Rf, Rb1, Rc, Rb2, and Rd. In the transformed P. ginseng samples, considerable amounts of deglycosylated metabolite compound K and Rh1 were detected. In addition, minimal amounts of deglycosylated metabolites (ginsenosides Rg2, F1, F2, Rg3, and protopanaxatriol-type ginsenosides) and untransformed ginsenosides Re, Rg1, and Rd were detected at 24 h. The results indicated that the primary metabolites are compound K and Rh1, and the protopanaxadiol-type ginsenosides were more easily metabolized than protopanaxatriol-type ginsenosides. Conclusion: This is the first report of the identification and quantification of the metabolism and metabolic profile of P. ginseng extract in rat intestinal microflora using LCeMS/MS. The current study provided new insights for studying the metabolism and active metabolites of P. ginseng.

Transverse Tensile Deformation and Failure of Three-dimensional Five-directional Braided Carbon Fiber Composites

Wei Zhou,Zhi-yuan Wei,Guang-fei Wang,Kang-ning Han,Ran Liu,Lian-Hua Ma 한국섬유공학회 2021 Fibers and polymers Vol.22 No.4

Accurate characterisation of transverse tensile deformation and damage evolution is of importance for evaluatingthe failure behaviors of three-dimensional (3D) braided composites. In the present study, a finite element method (FEM) andseveral non-destructive testing methods including acoustic emission, digital image correlation, and infrared thermography aredeveloped to investigate the transverse tensile deformation and damage evolution of 3D five-directional braided composites. In the finite element approach, a matrix-impregnated fiber bundles (MIFB) model and a representative volume cell (RVC)model, which take into account the fiber bundles and matrix, are respectively established to predict the effective mechanicalproperties of fiber bundles and simulate the deformation and progressive damage of such composites. The damaged locationsand the failure modes including matrix crack, fiber debonding and shear fracture of fiber are predicted and verified byexperimental tests. The non-destructive tests show that the transverse tensile fracture process can be divided into four stageswhich correspond to acoustic emission signals severally. The combination of the FEM based numerical modeling andmultiple non-destructive characterisation methods can accurately monitor the deformation and damage behaviors of 3Dbraided composites under transverse tensile loads and thus provide a reference for structural health monitoring of compositesin practical application.

intelligent

Wei Fei 한국콘텐츠학회 2020 ICCC International Digital Design Invitation Exhib Vol.2020 No.11

Appropriate nitrogen application enhances saponin synthesis and growth mediated by optimizing root nutrient uptake ability

Wei, Wei,Ye, Chen,Huang, Hui-Chuan,Yang, Min,Mei, Xin-Yue,Du, Fei,He, Xia-Hong,Zhu, Shu-Sheng,Liu, Yi-Xiang The Korean Society of Ginseng 2020 Journal of Ginseng Research Vol.44 No.4

Background: Cultivation of medicinal crops, which synthesize hundreds of substances for curative functions, was focused on the synthesis of secondary metabolites rather than biomass accumulation. Nutrition is an important restrict factor for plant growth and secondary metabolites, but little attention has been given to the plasticity of nutrient uptake and secondary metabolites synthesis response to soil nitrogen (N) change. Methods: Two year-field experiments of Sanqi (Panax notoginseng), which can synthesize a high level of saponin in cells, were conducted to study the effects of N application on the temporal dynamics of biomass, nutrient absorption, root architecture and the relationships between these parameters and saponin synthesis. Results: Increasing N fertilizer rates could improve the dry matter yields and nutrient absorption ability through increasing the maximum daily growth (or nutrient uptake) rate. Under suitable N level (225 kg/ha N), Sanqi restricted the root length and surface and enhanced the root diameter and N uptake rate per root length (NURI) to promote nutrient absorption, but the opposite status of Sanqi root architecture and NURI was found when soil N was deficient. Furthermore, increasing N rates could promote the accumulation of saponin in roots through improving the NURI, which showed a significant positive relationship with the content of saponin in the taproots. Conclusion: Appropriate N fertilizer rates could optimize both root architecture and nutrient uptake efficiency, then promote both the accumulation of dry matter and the synthesis of saponins.

Chemical Constituents from the Aerial Parts of Isodon coetsa and their Cytotoxicity

Wei Zhao,Jian Xin Pu,Xue Du,Yong Zhao,Fei He,Hai Bo Zhang,Yong Bo Xue,Wei Lie Xiao,Han Dong Sun,Ying Li Wu,Guo Qiang Chen 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.12

Three new compounds (1-3), including a neolignan, a triterpenoid, and a diterpenoid, together with twenty known compounds (4-23), were isolated from the aerial parts of Isodon coetsa. Their structures and relative configurations were elucidated on the basis of spectroscopic data. Compounds 1, 3, 5-9, 11-13, 16-17, and 19-23 were evaluated for their cytotoxicity against HT-29, BEL-7402, and SK-OV-3 human tumor cell lines. Compound 7 showed significant inhibitory effects on all three types of cells, with IC50 values of 2.52, 3.06, 2.14 μM, respectively.

Soil fungal communities of montane natural secondary forest types in China

Fei Cheng,Xin Wei,Lin Hou,Zhengchun Shang,Xiaobang Peng,Peng Zhao,Zhaoxue Fei,Shuoxin Zhang 한국미생물학회 2015 The journal of microbiology Vol.53 No.6

Distinctive plant communities may provide specific physical and chemical properties with soils by specific litters and root exudates to exert effects on soil microorganisms. Past logging activities in the Qinling Mountains induced diverse natural secondary forest types (NSFTs). How these recovered NSFTs regulate patterns of soil microbial communities remain limited. In the study, we used terminal-restriction fragment length polymorphism (T-RFLP) to precisely determine forest type-specific soil fungal diversity and composition in five NSFTs. Our results indicated that NSFTs had significant impacts on the soil fungal communities. The most diverse fungal species were found in the Armand pine (Pinus armandi) and Chinese pine (Pinus tabulaeformis) forest soils, followed by sharptooth oak (Quercus aliena var. acuteserrata) and Chinese pine-sharptooth oak forest soils, the wilson spruce (Picea wilsonii) forests had the lowest soil fungal diversity. The analyses of community composition suggested that the fungal communities of Armand pine forest soils were similar to those of Chinese pine forest soils, while other communities prominently differed from each other. Stepwise multiple regression analysis revealed that soil silt, clay, pH, and ammonium nitrogen had intimate linkages with soil fungal diversity. Furthermore, the patterns of soil fungal communites were strongly governed by the specific soil environments of the tested NSFTs, as described by canonical correspondence analysis (CCA). Finally, our study showed that soil fungal communities may be mediated by NSFTs via specific soil edaphic status. Hence, such a comparable study may provide fundamental information for fungal diversity and community structure of natural forests and assist with better prediction and understanding how soil fungal composition and function alter with forest type transformation.

Multi-objective Structural Optimization of Composite Wind Turbine Blade

Wei-Fei Hu(후웨이페이),Sang-Joon Yoon(윤상준),Sang-Chul Park(박상철),Dong-Hoon Choi(최동훈) 대한기계학회 2009 대한기계학회 춘추학술대회 Vol.2009 No.11

An extensively automated optimization procedure is presented for a Horizontal-Axis Wind Turbine (HAWT) blade. The design parameters associated with Glass Fiber Reinforced Plastics (GFRP) and Carbon Fiber Reinforced Plastics (CFRP) applied in HAWT blade is optimized automatically. The cost and total mass of the blade are set to be a multi-objective with the constraints of maximum tip deflection and Tsai-Wu failure criterion. To evaluate the mass, tip deflection and stress of the blade under pressure load, a FEM model is generated in SAMCEF environment, a commercial CAE tool. Combining it with a Process Integration and Design Optimization (PIDO) tool named PIAnO, the whole optimization process is executed automatically, which could dramatically reduce the manual design time and increase the efficiency in realistic industrial problem. The design variables consist of layer thickness, type of GFRP and CFRP layers as well as reinforcement orientations in composite laminate. Since they are all discrete values, Evolutionary Algorithm (EA) is applied for searching the global optimal solution with moderate calculation cost. The optimized stacking arrangement of CFRP and GFRP of the blade showed minimized cost and mass satisfying the tip deflection constraint and Tsai-Wu failure criterion.