[PD-0032] Targeted replacement of PhcA encoding transcription factor regulating multiple virulence genes in Ralstonia solanacearum infecting Solanum lycopersicum

Sung-Mi Kim(Sung-Mi Kim),Jinjeong Lee(Jinjeong Lee),Su Jung Ra(Su Jung Ra),Eunhee Kim(Eunhee Kim),Hong-Il Ahn(Hong-Il Ahn),Myeong Jin Kang(Myeong Jin Kang),HwangWeon Jeong(HwangWeon Jeong),Hye-Jin Yun 한국육종학회 2022 한국육종학회 공동학술발표집 Vol.2022 No.-

A Multi-Center Educational Research Regarding Breastfeeding for Pediatrics Residents in Korea

Yong Sung Choi,정성훈,김은선,Eun Sun Kim,Eunhee Lee,Euiseok Jung,So-Yeon Lee,이우령,Hye Sun Yoon,Yong Joo Kim,Ji Kyoung Park,Son Moon Shin,Ellen Ai-Rhan Kim 대한신생아학회 2022 Neonatal medicine Vol.29 No.1

Purpose: Pediatricians have a significant responsibility to educate mothers about the importance of breastfeeding. However, there have been minimal efforts in the courses of resident training in Korea. The purpose of this study is to evaluate the change in knowledge and attitude before and after a 4-week breastfeeding educational intervention among multicenter residents. Methods: Prospective interventional educational research was designed for residents at eight training hospitals in Korea. Institutional reviews were obtained in each hospital. The education curriculum consisted of 14 courses regarding breastfeeding theory and practice. These materials were used to teach pediatric residents for 4 weeks. Knowledge-based tests were administered before the course, and re-tests were administered after the course using different test items of similar levels. Test scores and survey responses were compared before and after the intervention. Results: A total of 73 residents (1st year 20, 2nd year 23, 3rd year 16, and 4th year residents 14) from eight training hospitals completed the intervention. Their average age was 30.3±2.9 years, 17 (23.3%) were male, 22 (30.1%) were married, and eight had more than one child of their own. The mean pre-test score was 61.8±13.4 and the mean post-test score was 78.3±7.5 (P<0.001). The inter-grade difference in the score was significant in the pre-test (P=0.005), but not significant in the post-test (P=0.155). There were more responses of obtaining confidence after the intervention (P<0.001). Conclusion: In our study, pediatric residents showed improvement in their knowledge and confidence level after 4 weeks of the breastfeeding curriculum. This will provide a basis for future policymaking in the training of pediatric residents regarding breastfeedReceived: 6 January 2022 Revised: 15 February 2022 Accepted: 15 February 2022 Correspondence to: Ellen Ai-Rhan Kim, MD Department of Pediatrics, Asan Medical Center, University of Ulsan College of Medicine, 88 Olympic-ro 43-gil, Songpa-gu, Seoul 05505, Korea Tel: +82-2-3010-3390 Fax: +82-2-3010-6978 E-mail: arkim@amc.seoul.kr A Multi-Center Educational Research Regarding Breastfeeding for Pediatrics Residents in Korea Yong-Sung Choi, MD,PhD1, Sung-Hoon Chung, MD, PhD2, Eun Sun Kim, MD, PhD3, Eun Hee Lee, MD4, Euiseok Jung, MD5, So Yeon Lee, MD, PhD5, Wooryoung Lee, MD6, Hye Sun Yoon, MD, PhD7, Yong Joo Kim, MD, PhD8, Ji Kyoung Park, MD, PhD9, Son Moon Shin, MD, PhD9, and Ellen Ai-Rhan Kim, MD, PhD5 1Department of Pediatrics, Kyung Hee University Hospital, Seoul, Korea 2Department of Pediatrics, Kyung Hee University Hospital at Gangdong, Seoul, Korea 3Department of Pediatrics, Kangwon National University Hospital, Chuncheon, Korea 4Department of Pediatrics, Korea University Anam Hospital, Seoul, Korea 5Department of Pediatrics, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea 6Department of Pediatrics, Soonchunhyang University Seoul Hospital, Seoul, Korea 7Department of Pediatrics, Nowon Eulji Medical Center, Eulji University, Seoul, Korea 8Department of Pediatrics, Hanyang University Seoul Hospital, Seoul, Korea 9Department of Pediatrics, Inje University Busan Paik Hospital, Busan, Korea Neonatal Med 2022 February;29(1):28-35 https://doi.org/10.5385/nm.2022.29.1.28 pISSN 2287-9412 . eISSN 2287-9803 Copyright(c) 2022 By Korean Society of Neonatology This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http:// creativecommons.org/licenses/by-nc/4.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Original Article 29 Neonatal Med 2022 February;29(1):28-35 https://doi.org/10.5385/nm.2022.29.1.28 ing in Korea.

Strategies and Advancement in Antibody-Drug Conjugate Optimization for Targeted Cancer Therapeutics

Kim, Eunhee G.,Kim, Kristine M. The Korean Society of Applied Pharmacology 2015 Biomolecules & Therapeutics(구 응용약물학회지) Vol.23 No.6

Antibody-drug conjugates utilize the antibody as a delivery vehicle for highly potent cytotoxic molecules with specificity for tumor-associated antigens for cancer therapy. Critical parameters that govern successful antibody-drug conjugate development for clinical use include the selection of the tumor target antigen, the antibody against the target, the cytotoxic molecule, the linker bridging the cytotoxic molecule and the antibody, and the conjugation chemistry used for the attachment of the cytotoxic molecule to the antibody. Advancements in these core antibody-drug conjugate technology are reflected by recent approval of Adectris$^{(R)}$(anti-CD30-drug conjugate) and Kadcyla$^{(R)}$(anti-HER2 drug conjugate). The potential approval of an anti-CD22 conjugate and promising new clinical data for anti-CD19 and anti-CD33 conjugates are additional advancements. Enrichment of antibody-drug conjugates with newly developed potent cytotoxic molecules and linkers are also in the pipeline for various tumor targets. However, the complexity of antibody-drug conjugate components, conjugation methods, and off-target toxicities still pose challenges for the strategic design of antibody-drug conjugates to achieve their fullest therapeutic potential. This review will discuss the emergence of clinical antibody-drug conjugates, current trends in optimization strategies, and recent study results for antibody-drug conjugates that have incorporated the latest optimization strategies. Future challenges and perspectives toward making antibody-drug conjugates more amendable for broader disease indications are also discussed.

Sonographic features of follicular variant papillary thyroid carcinomas in comparison with conventional papillary thyroid carcinomas.

Kim, Dae Sik,Kim, Ji-hoon,Na, Dong Gyu,Park, Sung-Hye,Kim, Eunhee,Chang, Kee-Hyun,Sohn, Chul-Ho,Choi, Young Ho W.B. Saunders 2009 Journal of ultrasound in medicine Vol.28 No.12

<P>OBJECTIVE: The purpose of this study was to compare the sonographic features as well as the results of fine-needle aspiration biopsy (FNAB) of follicular variant papillary thyroid carcinoma (FVPTCs) and conventional papillary thyroid carcinoma (PTCs). METHODS: Forty patients with 44 FVPTCs and 59 patients with 74 conventional PTCs were enrolled in this study. The sonographic features, sonographic gradings, and FNAB results were compared between the two groups. RESULTS: The mean nodule size of FVPTCs was larger than that of conventional PTCs (17.70 versus 10.53 mm; P < .001). Sonographic features of an ovoid-to-round shape (95% versus 73%), isoechogenicity (52% versus 8%), and a hypoechoic halo (25% versus 3%) were more frequent in FVPTCs than conventional PTCs (P < .001). Sonographic features of a taller-than-wide shape (5% versus 22%), a spiculated margin (7% versus 32%), marked hypoechogenicity (5% versus 38%), and microcalcification (7% versus 24%) were rarer in FVPTCs than conventional PTCs (P < .05). The incidence of a sonographically malignant grade was also lower in FVPTCs (48%) than conventional PTCs (81%; P < .001). A diagnosis of PTC on FNAB of FVPTCs was less common than that of conventional PTCs (28% versus 56%; P = .0393); however, a diagnosis of an indeterminate cytologic type such as atypical cells or follicular lesions in FVPTCs was higher than that in conventional PTCs (46% versus 19%; P = .0418). CONCLUSIONS: Follicular variant papillary thyroid carcinomas show a relatively larger size, more benign sonographic features, a lower incidence of a sonographically malignant grade, and a lower diagnostic rate of PTC on FNAB compared with conventional PTCs.</P>

Reviews : Invited Review ; Strategies and Advancement in Antibody-Drug Conjugate Optimization for Targeted Cancer Therapeutics

( Eunhee G Kim ),( Kristine M Kim ) 한국응용약물학회 2015 Biomolecules & Therapeutics(구 응용약물학회지) Vol.23 No.6

Antibody-drug conjugates utilize the antibody as a delivery vehicle for highly potent cytotoxic molecules with specificity for tumor-associated antigens for cancer therapy. Critical parameters that govern successful antibody-drug conjugate development for clinical use include the selection of the tumor target antigen, the antibody against the target, the cytotoxic molecule, the linker bridging the cytotoxic molecule and the antibody, and the conjugation chemistry used for the attachment of the cytotoxic molecule to the antibody. Advancements in these core antibody-drug conjugate technology are reflected by recent approval of Adectris® (anti-CD30-drug conjugate) and Kadcyla® (anti-HER2 drug conjugate). The potential approval of an anti-CD22 conjugate and promising new clinical data for anti-CD19 and anti-CD33 conjugates are additional advancements. Enrichment of antibody-drug conjugates with newly developed potent cytotoxic molecules and linkers are also in the pipeline for various tumor targets. However, the complexity of antibody-drug conjugate components, conjugation methods, and off-target toxicities still pose challenges for the strategic design of antibody-drug conjugates to achieve their fullest therapeutic potential. This review will discuss the emergence of clinical antibody-drug conjugates, current trends in optimization strategies, and recent study results for antibody-drug conjugates that have incorporated the latest optimization strategies. Future challenges and perspectives toward making antibody-drug conjugates more amendable for broader disease indications are also discussed.

Fas-associated factor 1 mediates NADPH oxidase-induced reactive oxygen species production and proinflammatory responses in macrophages against Listeria infection

Kim, Tae-Hwan,Lee, Hyun-Cheol,Kim, Jae-Hoon,Hewawaduge, C. Y.,Chathuranga, Kiramage,Chathuranga, W. A. Gayan,Ekanayaka, Pathum,Wijerathne, H. M. S. M.,Kim, Chul-Joong,Kim, Eunhee,Lee, Jong-Soo Public Library of Science 2019 PLoS pathogens Vol.15 No.8

<P> Fas-associated factor 1 is a death-promoting protein that induces apoptosis by interacting with the Fas receptor. Until now, FAF1 was reported to interact potentially with diverse proteins and to function as a negative and/or positive regulator of several cellular possesses. However, the role of FAF1 in defense against bacterial infection remains unclear. Here, we show that FAF1 plays a pivotal role in activating NADPH oxidase in macrophages during <I>Listeria monocytogenes</I> infection. Upon infection by <I>L. monocytogenes,</I> FAF1 interacts with p67phox (an activator of the NADPH oxidase complex), thereby facilitating its stabilization and increasing the activity of NADPH oxidase. Consequently, knockdown or ectopic expression of FAF1 had a marked effect on production of ROS, proinflammatory cytokines, and antibacterial activity, in macrophages upon stimulation of TLR2 or after infection with <I>L. monocytogenes.</I> Consistent with this, FAF1<SUP>gt/gt</SUP> mice, which are knocked down in FAF1, showed weaker inflammatory responses than wild-type mice; these weaker responses led to increased replication of <I>L. monocytogenes.</I> Collectively, these findings suggest that FAF1 positively regulates NADPH oxidase-mediated ROS production and antibacterial defenses. </P>

Rapid Purification and Characterization of Nucleoside Diphosphate Kinase Isoforms Using ATP-Sepharose Affinity Column Chromatography

Kim, Sun Young,Chang, Keun Hye,Doh, Hyun-Joo,Jung, Jin A,Kim, Eunhee,Sim, Chung Ja,Lee, Kong-Joo 梨花女子大學校 藥學硏究所 1998 藥學硏究論文集 Vol.- No.7

Nucleoside diphosphate kinases (NDP kinases), products of the nm23 gene, catalyze the transfer of the terminal phosphate group of the nucleoside triphosphate to the corresponding diphosphate and may be involved in tumor metastasis suppression, development, and signal transduction. NDP kinases from various sources including human erythrocytes, rat brain tissue and E. coli strain BL21 transformed with pET3C expression plasmids containing nm23-H1 or nm23-H2, were purified in one step to homogeneity using ATP-sepharose affinity column chromatography. This method was applicable for the purification of various NDP kinases which show the same enzymatic activity and immunodetection, but have various molecular weight and quaternary structures.

MALDI-TOF Mass Spectrometric Analysis of Chemical Warfare Nerve Agent Simulants

Eunhee Kim,Hyunji Lee,Sun-Kyung Choi,윤명한,오한빈 대한화학회 2016 Bulletin of the Korean Chemical Society Vol.37 No.3

To avoid possible danger, simulants of chemical warfare nerve gas agents, such as dimethyl methylphosphonate (DMMP), triethyl phosphate (TEP), di(propylene glycol) monomethyl ether (DPGMME), methyl salicylate, and diethyl phthalate (DEP), are often used for the development of a new detection and analysis method. In the present study, several MALDI matrices were evaluated to determine the optimal matrix for the simultaneous Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) detection of the above-mentioned five chemical warfare agent (CWA) simulants, and α-cyanohydroxycinnamic acid (CHCA) was found to be most effective in detecting all of the simulants without interference from strong background matrix peaks. Furthermore, the limits of detection for the five simulants were roughly estimated with the CHCA matrix. This information is expected to be important for the development of future electrowetting-on-dielectrics (EWOD)-based sample preparation methods that can be subjected to subsequent analysis by MALDI-TOF MS.

On the synthesis of a hierarchically-structured ZSM-5 zeolite and the effect of its physicochemical properties with Cu impregnation on cold-start hydrocarbon trap performance

Kim, Heejoong,Jang, Eunhee,Jeong, Yanghwan,Kim, Jinseong,Kang, Chun Yong,Kim, Chang Hwan,Baik, Hionsuck,Lee, Kwan-Young,Choi, Jungkyu Elsevier 2018 CATALYSIS TODAY - Vol.314 No.-

<P><B>Abstract</B></P> <P>A hierarchically structured zeolite (self-pillared pentasil; SPP) comprised of MFI nanosheets or lamellae has been synthesized in various Si/Al ratios and mesoporosities. It turns out that a simple removal of ethanol in a synthesis sol resulted in increased mesoporosity, while the additional reduction of water further increased mesoporosity. In addition, we could synthesize the SPP particle with the actual Si/Al ratio as low as ∼23 with a modest mesoporosity. With these hierarchically structured SPP particles, we further conducted copper impregnation on them in order to use as a hydrocarbon (HC) trap. The resulting Cu-impregnated SPPs could not only adsorb HCs in the exit gas stream including water vapor, but also serve as an active oxidizer of HCs. Specifically, Cu-impregnated SPP with an actual Si/Al ratio of ∼22 and medium mesoporosity exhibited very high performance in cold-start trap tests; desirably adsorbing propene and toluene even in the presence of 10 vol% steam, holding them up to higher temperatures (90 °C for propene and 190 °C for toluene), and furthermore, oxidizing the hydrocarbons. The preferred adsorption can be attributed to the larger amount of exchanged Cu<SUP>2+</SUP> ions in SPP particles with a lower Si/Al ratio, while the additional oxidation was due to the CuO particles dispersed on the SPP surface. However, the hydrothermal stability test revealed that the zeolite structure in the Cu-impregnated SPPs was collapsed and transformed into another undesired phase, thus losing the above-mentioned adsorption ability. Nevertheless, the corresponding oxidation performance was well maintained, indicating the robust, active role of the CuO particles.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The mesoporosity in SPP particles was increased by removing ethanol and water. </LI> <LI> Mesoporous SPP particles with a Si/Al ratio as low as ∼23 could be synthesized. </LI> <LI> Cu-impregnated SPPs were effective for eliminating hydrocarbon (HC) in a cold start. </LI> <LI> The effect of physicochemical properties of SPPs on the HC trap was investigated. </LI> <LI> Cu<SUP>2+</SUP> ions increased HC adsorption, while CuO contributed to HC oxidation. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Apoptosis-inducing factor (AIF) inhibits protein synthesis by interacting with the eukaryotic translation initiation factor 3 subunit p44 (eIF3g)

Kim, Jong-Tae,Kim, Kwang Dong,Song, Eun Young,Lee, Hee Gu,Kim, Jae Wha,Kim, Jung Woo,Chae, Suhn-Kee,Kim, Eunhee,Lee, Myeong-Sok,Yang, Young,Lim, Jong-Seok Elsevier 2006 FEBS letters Vol.580 No.27

<P><B>Abstract</B></P><P>Apoptosis-inducing factor (AIF) is a ubiquitous FAD-binding flavoprotein comprised of 613 amino acids and plays an important role in caspase-independent apoptosis. During apoptotic induction, AIF is translocated from the mitochondrial intermembrane space to the nucleus, where it interacts with DNA and activates a nuclear endonuclease. By performing a yeast two-hybrid screen with mature AIF, we have isolated the eukaryotic translation initiation factor 3 subunit p44 (eIF3g). Our deletion mutant analysis revealed that the eIF3g N-terminus interacts with the C-terminal region of AIF. The direct interaction between AIF and eIF3g was confirmed in a GST pull-down assay and also verified by the results of co-immunoprecipitation and confocal microscopy studies. Using an in vitro TNT coupled transcription–translation system, we found that mature AIF could inhibit newly-translated protein synthesis and this inhibition was significantly blocked by eIF3g competitively. These results were also confirmed in cells. In addition, mature AIF overexpression specifically resulted in the activation of caspase-7, thereby amplifying the inhibition of protein synthesis including eIF3g cleavage. Our data suggest that eIF3g is one of the cytosolic targets that interacts with mature AIF, and provide insight into the AIF’s cellular functions of the inhibition of protein synthesis during apoptosis.</P>