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      • KCI등재

        Response of fruit set and quality and seed formation to ploidy levels of pollen donor in yellow-fl eshed kiwifruits

        Eun Ui Oh,Seung Yong Jeong,Ju Young Kim,송관정 한국원예학회 2021 Horticulture, Environment, and Biotechnology Vol.62 No.1

        This study investigated how the ploidy level of the kiwifruit pollinizer cultivars “CK3” (diploid), “T line” (tetraploid),“Pohwa” (hexaploid) and “Chieftain” (hexaploid) aff ected fruit set, fruit quality, and seed formation in the tetraploid kiwifruitcultivars “Halla Gold” and “Sweet Gold” cultivated in an unheated greenhouse in Jeju, Korea. Pollen tubes growingin the pistil reached and combined with the ovule 3 days after artifi cial pollination, and their patterns diff ered depending onthe ploidy level of the pollen parent. The number of pollen tubes observed in “Halla Gold” and “Sweet Gold” pistils wassignifi cantly lower following pollination by “CK3” than with the other pollen donors. In all pollen treatments, the fruit setrates were > 90%. The fruit weight of both “Halla Gold” and “Sweet Gold” were high following pollination with “Chieftain”and “Pohwa”. The dry matter content, soluble solids, and acidity were not signifi cantly diff erent among all pollinationtreatments. Fruit fi rmness was higher following pollination with “Chieftain” and “Powha.” Colorimeter h ˚ values for fl eshof “Halla Gold” was low following pollination with “CK3,” but there were no diff erences for “Sweet Gold” among all pollinations. The number of seeds showed a similar trend to fruit weight, but the 100-seed weight was highest with “T line” asthe pollinizer. The results indicate that the ploidy level of the pollen donor aff ects fruit quality more than fruit set. Also, thepollen most suitable for cultivation of “Halla Gold” and “Sweet Gold” is considered to be tetraploid “T line” and hexaploid“Pohwa” and “Chieftain,” which showed favorable eff ects on the weight and fi rmness of fruits, and the number and weightof seeds without adversely infl uencing fruit set and dry matter content.

      • KCI등재

        Characterization of Pollen Development in Staminate Kiwifruit (Actinidia sp.) Cultivars

        Eun Ui Oh,Seung Yong Jeong,Hyeong Ho Kang,Kwan Jeong Song 한국원예학회 2020 원예과학기술지 Vol.38 No.1

        This study was conducted to determine the timing of flower harvest for pollen collection and preparation in kiwifruit. Here the pollen development of two staminate cultivars, ‘Bohwa’ and ‘Chieftain’, which are the major pollinizers used in the region of Jeju in Korea, was investigated. These two staminate cultivars showed a similar tendency in histological pollen development, except for the time of anther dehiscence. The highest pollen quantity was obtained at the 4th developmental stage; thereafter, the quantity of pollen decreased by 23% and 38% at the 5th developmental stage in ‘Bohwa’ and ‘Chieftain’, respectively. The pollen viability of ‘Bohwa’ was slightly higher than that of ‘Chieftain’ in both 1% iodine potassium iodide and fluorescein diacetate staining with a small increase according to progression in flower development from the 1st to the 5th stage. Pollen germination also increased as flower development advanced, and higher pollen germination of more than 60% occurred in the 4th developmental stage of both staminate cultivars. The results indicate that the appropriate timing for pollen collection might be the 4th developmental stage (full balloon stage) in ‘Bohwa’ and ‘Chieftain’ according to the amount of pollen production, viability, and germination.

      • KCI등재후보

        감귤에서 대목에 의한 과실 품질 특성의 변화

        오은의(Oh, Eun-Ui),김호방(Kim, Ho-Bang),송관정(Song, Kwan-Jeong) 제주학회 2021 濟州島硏究 Vol.56 No.-

        우리나라의 감귤산업에서 최근 개발된 새로운 품종들에 적합한 대목의 이용을 촉진 하고자 대목의 역할과 기능에 대해 고찰하였다. 본고에서는 대목의 역할과 중요성, 국내는 물론 세계적으로 이용되고 있는 대목의 종류와 특성, 대목에 의한 접수 품종의 과실 품질 특성의 변화와 생리적 기작에 대해 제시하였다. 대목은 품종의 토양 적응성, 바이러스 저항성 및 왜화성의 역할은 물론 과실 품질에도 영향을 주기 때문에 중요하 다. 그러므로 지역의 특성에 따라 이용하는 대목의 종류가 달라지며, 우리나라는 거의 대부분 탱자를 이용하고 있다. 최근 다양한 신품종들이 개발됨에 따라, 품종의 특성 발현을 최적화할 수 있는 대목 개발이 요구되고 있다. 특히 과실 경쟁력의 핵심 요인인 당도, 산도, 크기, 색발현, 기능성 성분 등의 특성이 대목에 따라 달라질 수 있다. 이는 대목의 발현 유전자 산물들이 접목부를 건너 이동하여 품종의 유전자 발현을 변화시키 고, 과실 품질의 변화로 이어지게 된다. 향후 대목의 기능에 대한 이해가 확산되어 품종에 적합한 대목의 선발 및 이용으로 감귤산업의 경쟁력 제고로 이어질 수 있기를 기대한다. The review on the role and function of rootstocks was conducted with an aims to encourage the use of rootstocks proper to new scion cultivars developed recently in Korean citrus industry. This paper represented a role and importance of rootstocks, the kind and characteristics of rootstocks used worldwide as well as nationally, and the changes of scion fruit quality and physiological mechanism affected by rootstocks. The rootstock is important due to attributes affecting not only scion tree adaptation to soils, tolerance to virus infection, and dwarfness, but also the fruit quality of scion trees. Therefore, the different kind of rootstocks is selected for cultivation environment and trifoliate orange is mostly used in Korea. The release of new scion cultivars has been recently increased, which needs for introducing rootstocks suitable to optimize scion tree performance. Specially, major factors such as soluble solids, acidity, fruit size, pulp pigmentation, bioactive components, etc. for determining fruit competitiveness might be different from those of rootstocks being used. Some transcripts and metabolites of rootstock are transported across grafting site to change the gene expression and fruit quality of scion trees. The review could contribute to improve the competitiveness of citrus industry through promoting the selection and use of suitable rootstocks based on a full understanding on the functions of rootstock.

      • KCI등재

        동질 사배체 탱자에서 염색체 배가와 수체 표현형의 변이

        오은의(Eun Ui Oh),채치원(Chi-Won Chae),김샛별(Sat-Byul Kim),Jian Liang Lu,윤수현(Su-Hyun Yun),고상욱(Sang-Wook Koh),송관정(Kwan Jeong Song) 한국원예학회 2014 원예과학기술지 Vol.32 No.3

        이배체 탱자로부터 자연적으로 발생한 동질 사배체 탱자의 수체 형질 관련 표현형 및 유전체 메틸화 변이 정도를 분석하여 후성 유전의 하나인 유전체 메틸화가 동질 사배체의 표현형 변이의 요인으로 작용할 수 있음을 구명하고자 본 실험을 수행하였다. 이배체 탱자에서 유래된 14주의 사배체 탱자로부터 염색체를 분석하여 이수성이 없는 2n = 4X = 36 식물체로 확인하였다. CMA 핵형 분석 결과 염색체가 배가된 동질 사배체임을 확인할 수 있었다. 동질 사배체에서 수고, 수폭, 원가지 수, 원가지 길이, 분지 각도, 마디 길이, 잎의 특성 등 동질 사배체 수체 표현형에 있어서 상당한 변이가 나타남을 확인하였다. 또한 동질 사배체 광합성률에는 큰 차이는 없었지만, SPAD 값에 의한 엽록소 지수에 있어서도 표현형이 다양하게 나타나는 것을 알 수 있었다. 그 외에도 기공 밀도와 공변 세포 길이에 있어서 광범위하게 변이가 관찰되는 것을 알 수 있었다. Global cytosine DNA 메틸화를 분석한 결과 개체 간 메틸화 정도에 차이가 존재함을 알 수 있었다. 동질 사배체 탱자 14주의 절반이 이배체 탱자의 메틸화와 비교하였을 때 2배 이상으로 나타난 것을 확인하였다. 본 연구 결과 동질 사배체에서 나타나는 수체형질 변이가 gene redundency를 줄이기 위한 global cytosine DNA 메틸화와 관계될 수 있음을 확인하였다. The study was conducted to investigate the possibility that epigenetic DNA methylation causes tree phenotypic variation in autotetraploids through evaluating the phenotypic variation and DNA methylation in autotetraploids occurred spontaneously from diploid trifoliate orange. Chromosome analysis confirmed that fourteen trifoliate orange trees of selected by flow cytometry were tetraploids (2n = 4X = 36) without any aneuploids. Chromomycin A3 staining determined that these trees were all autotetraploid with doubled chromosome set. Tree phenotypes, such as tree height and width, branching number, length, and angle, internode length, and leaf characteristics, varied in the autotetraploids. Chlorophyll indices were diverse in the autotetraploids, but photosynthetic rates were not significantly different. In addition, a wide range of variation was observed in stomatal density and guard cell length. Analysis of global cytosine DNA methylation showed that there was a variation of the methylation level in autotetraploids. More than half of 14 autotetraploids had at least 2 times higher methylation level than diploid trifoliate orange. The results indicate that tree phenotypic variation in autotetraploids might be related to global DNA methylation for reducing gene redundancy.

      • A Genetic Linkage Map based on AFLP markers in China type Tea Plant

        Yali Chang,EunUi Oh,Min‐Seuk Lee,Kwan‐Jeong Song 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07

        Based on double pseudo-testcross theory, a population of 76 F1 clones, which were derived from a cross of China type tea plants (Camellia sinensis var. sinensis) with a Korean tea cultivar, ‘Kemsull’ for female parent and a Japanese tea cultivar, ‘Houshun’ for male parent, was used to construct a genetic linkage map with AFLP markers. Totally, 2,360 markers were detected by 26 pairs of primers and 90.8 markers for each pair on average. Among these, 481 markers (20.3%) were polymorphic, 392 markers (81.5%) of which showed Mendelian segregation ratio (p=0.01). Of these Mendelian segregated markers, 139 (35.5%) were segregated in 3:1 ratio and 253 (65.5%) were segregated in 1:1 ratio. The construction of AFLP molecular marker based linkage map were carried out by Joinmap 4.0 version. The linkage map of ‘Kemsull’ contained 227 markers which distributed into 18 linkage groups. The linkage map of ‘Kemsull’ covered 1,382.2 cM with the average distance between two markers of 6.0 cM. The linkage map of ‘Houshun’ contained 154 markers which were distributed into 17 linkage groups and were spanned with the total map length of 1,540.9 cM and the average distance between two markers of 10 cM. However, these AFLP markers were not distributed evenly and further even saturation is additionally required.

      • KCI등재

        Hip Muscle Strength and Ratio Differences in Delivery Workers With and Without Iliotibial Band Syndrome

        Eun-su Lee,Ui-jae Hwang,Hwa-ik Yoo,Il-kyu Ahn,Oh-yun Kwon 한국전문물리치료학회 2023 한국전문물리치료학회지 Vol.30 No.1

        Background: Delivery workers repeatedly get in and out of trucks and walk or run to deliver packages during work. Iliotibial band syndrome (ITBS) is a well-known non-traumatic overuse injury of the lateral side of the knee caused by frequent knee flexion and extension. Hip muscle strength is among the factors that prevent lower extremity injuries. Although many studies have examined the relationship between ITBS and hip muscle strengths, there was no study comparing hip muscle strength and ratio between delivery workers with and without ITBS. Objects: This study aimed to compare hip muscle strength and hip internal/external rotator and adductor/abductor strength ratios between delivery workers with and without ITBS. Methods: Fourteen delivery workers with ITBS matched inclusion criteria in the present study among 20 participants. Because total sample size was required 28 subjects by G*power pro-gram (ver. 3.1.9.4; University of Trier), 14 delivery workers without ITBS were recruited. Hip muscle strengths were measured in a side-lying position using a Smart KEMA pulling sensor (KOREATECH Co. Ltd.). An independent t-test was used to compare hip muscle strengths and hip internal/external rotator and hip adductor/abductor strength ratios between delivery work-ers with and without ITBS. Results: The adductor/abductor strength ratio was significantly greater in delivery workers without ITBS than in those with ITBS (p < 0.05). The strengths of the hip abductor, hip adductor, hip internal rotator, hip external rotator, and the ratio of internal/external rotator strengths were not significantly different between the delivery workers with and without ITBS (p > 0.05). Conclusion: This study’s findings showed that delivery workers with ITBS had significantly lesser adductor/abductor strength ratio, while the strengths of the hip abductor and adduc-tor muscles did not differ significantly. These results suggest that adductor/abductor strength ratio should be considered when evaluating and treating ITBS in delivery workers.

      • KCI등재

        Comprehensive and synthetic inventory of Dokdo Island, Republic of Korea

        Ui Wook Hwang,Hyun Soo Rho,Bia Park,Eun Hwa Choi,Cho Rong Shin,Sa Heung Kim,Jongrak Lee,Hack Cheul Kim,Mann Kyoon Shin,Taeseo Park,Jumin Jun,Heegab Lee,Jong Eun Lee,Yoon Sik Oh,Jung-Goo Myoung,Chang G The National Institute of Biological Resources 2023 Journal of species research Vol.12 No.no.spc

        This study aims to establish a comprehensive, synthetic inventory system for the fauna and flora of Dokdo Island, Republic of Korea, which has been conducted by a specialized research group consisting of more than 50 experts. The research was conducted over five years(2015-2019) and supported by the National Institute of Biological Resources, Ministry of Environment, Republic of Korea. All possible publications on the fauna and flora of Dokdo Island over the last 68 years from 1952 to 2020 were reviewed. As a result, 1,302 species were found on Dokdo Island during the study period. An updated list of 1,963 species was created. This is expected to be of great help for the conservation and national publicity of important indigenous biological resources of Dokdo Island.

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