Linkage Disequilibrium (LD) Mapping and Tagging SNP Selection of C-Fos Induced Growth Factor (Figf) Gene in Korean Population

Kim, Sook,Yoo, Yeon-Kyung,Jang, Hye-Yoon,Shin, Eun-Soon,Cho, Eun-Young,Kim, Eu-Gene,NamKung, Jung-Hyun,Yang, Jun-Mo,Lee, Jong-Eun The Korean Society of Toxicogenomics and Toxicopro 2006 Molecular & cellular toxicology Vol.2 No.1

We performed comprehensive SNP validation and linkage disequilibrium (LD) analysis of the c-fos induced growth factor (Figf) gene in Korean population. Out of 32 SNPs, only 9 SNPs were polymorphic in Korean population. Validated SNPs formed a single extended haplotype block with strong LD through the entire length of the gene. Tagging SNP analysis picked only 2 SNPs to represent most of the genetic variation information of the Figf gene. Our results demonstrate the utility of LD block and tagging SNP analysis for an efficient way of performing a candidate gene based association study.

Human Immunodeficiency Virus-1 Tat 단백에 의한 인간 CD99유전자의 조절기전에 대한 연구

이유진,김예리,이미경,이임순,Lee, Eu-Gene,Kim, Ye-Ri,Lee, Mi-Kyung,Lee, Im-Soon 한국미생물학회 2008 미생물학회지 Vol.44 No.4

HIV에 감염된 환자의 경우 다양한 종류의 암이 발생하는 것으로 알려져 있다. 이러한 암종의 높은 발생률의 원인으로, 감염에 의한 면역세포의 감소 및 결핍과 같은 간접적인 이유 뿐 아니라, HIV 바이러스 단백질의 발현이 직접적으로 병의 발생에 관여한다는 보고가 있다. 본 연구에서는 HIV 환자에서 높게 나타나는 암의 발생에 대한 기전을 이해하기 위하여 HIV-1 Tat 유전자와, 다수의 암 발생과 관련이 있는 세포막단백 CD99와의 관계를 규명하였다. 먼저 CD99의 발현에 미치는 Tat의 영향을 알아보기 위하여 HIV-1 Tat 발현 안정화 세포주를 확립하고 Tat 단백에 의한 CD99 유전자의 발현 양상 변화를 분석하였다. 실험결과 Tat의 발현에 의하여 CD99 유전자의 발현이 활성화되는 것이 관찰되었으며 이와 반대로 STAT3의 발현은 낮아졌다. CD99 프로모터는 CpG 함량이 높기 때문에 Tat 단백이 DNA 메칠화를 통해서 CD99 유전자의 발현을 조절하는지 확인하기 위하여 methylation specific PCR을 수행하였고 Tat의 발현이 높은 곳에서 특이적으로 CD99 프로모터 부위가 탈메칠화되는 것을 발견하였다. Tat 발현 세포에서만 특이적인 발현 차이를 보이는 유전자 분석을 위한 Differentially Expressed Gene keratin 17과 collagen, type IV 증가됨이 확인되었다. 위의 결과는 HIV Tat 단백이 직접 세포 단백들을 조절하여 암을 발생시킬 수 있다는 보고를 뒷받침한다. HIV affects many organ systems. Patients with HIV infection have substantially increased risk of developing various cancers, primarily by opportunistic infection with oncogenic viruses due to their immunocompromised status. However, extensive evidence also indicates that the viral protein, Tat itself, may playas a major factor in the development of AIDS-related neoplasms. The molecular mechanism underlying Tat's oncogenic activity may include deregulation of cellular genes. Therefore, in this study, we examined the effect of HIV-l Tat on CD99 as one of the target cellular genes, which is a well-known tumor marker in several cancers. By using established HeLa clones that are stably expressing Tat, we found that CD99 is upregulated by endogenous Tat, whereas STAT3 is down regulated. Upon the screening of genes differentially expressed between Tat-stable cells and the control cells by using the gene fishing technique, DEG, we detected 3 genes which expression is affected by the presence of Tat. Furthermore, the methylation specific PCR analysis of the stably Tat expressing cell lines revealed that the CD99 promoter is de methylated in the presence of Tat. Taken together, these results open a potential role of CD99 in AIDS-related oncogenesis via epigenetic regulation by HIV-1 Tat.

Quantitative PCR for Etiologic Diagnosis of Methicillin-Resistant Staphylococcus aureus Pneumonia in Intensive Care Unit

( Sun Jung Kwon ),( Taeh Yeon Jeon ),( Dong Wook Seo ),( Moon Joon Na ),( Eu Gene Choi,),( Ji Woong Son ),( Eun Hyung Yoo ),( Chang Gyo Park ),( Hoi Young Lee ),( Ju Ock Kim ),( Sun Young Kim ),( Jae 대한결핵 및 호흡기학회 2012 Tuberculosis and Respiratory Diseases Vol.72 No.3

Background: Ventilator-associated pneumonia (VAP) requires prompt and appropriate treatment. Since methicillin- resistant Staphylococcus aureus (MRSA) is a frequent pathogen in VAP, rapid identification of it, is pivotal. Our aim was to evaluate the utility of quantitative polymerase chain reaction (qPCR) as a useful method for etiologic diagnoses of MRSA pneumonia. Methods: We performed qPCR for mecA, S. aureus-specific femA-SA, and S. epidermidis-specific femA-SE genes from bronchoalveolar lavage or bronchial washing samples obtained from clinically-suspected VAP. Molecular identification of MRSA was based on the presence of the mecA and femA-SA gene, with the absence of the femA-SE gene. To compensate for the experimental and clinical conditions, we spiked an internal control in the course of DNA extraction. We estimated number of colony-forming units per mL (CFU/mL) of MRSA samples through a standard curve of a serially-diluted reference MRSA strain. We compared the threshold cycle (Ct) value with the microbiologic results of MRSA. Results: We obtained the mecA gene standard curve, which showed the detection limit of the mecA gene to be 100 fg, which corresponds to a copy number of 30. We chose cut-off Ct values of 27.94 (equivalent to 1×104 CFU/mL) and 21.78 (equivalent to 1×105 CFU/mL). The sensitivity and specificity of our assay were 88.9% and 88.9% respectively, when compared with quantitative cultures. Conclusion: Our results were valuable for diagnosing and identifying pathogens involved in VAP. We believe our modified qPCR is an appropriate tool for the rapid diagnosis of clinical pathogens regarding patients in the intensive care unit.

Quantitative PCR for Etiologic Diagnosis of Methicillin-Resistant Staphylococcus aureus Pneumonia in Intensive Care Unit

Kwon, Sun-Jung,Jeon, Tae-Hyeon,Seo, Dong-Wook,Na, Moon-Joon,Choi, Eu-Gene,Son, Ji-Woong,Yoo, Eun-Hyung,Park, Chang-Gyo,Lee, Hoi-Young,Kim, Ju-Ock,Kim, Sun-Young,Kang, Jae-Ku The Korean Academy of Tuberculosis and Respiratory 2012 Tuberculosis and Respiratory Diseases Vol.72 No.3

Background: Ventilator-associated pneumonia (VAP) requires prompt and appropriate treatment. Since methicillin-resistant Staphylococcus aureus (MRSA) is a frequent pathogen in VAP, rapid identification of it, is pivotal. Our aim was to evaluate the utility of quantitative polymerase chain reaction (qPCR) as a useful method for etiologic diagnoses of MRSA pneumonia. Methods: We performed qPCR for mecA, S. aureus-specific femA-SA, and S. epidermidis-specific femA-SE genes from bronchoalveolar lavage or bronchial washing samples obtained from clinically-suspected VAP. Molecular identification of MRSA was based on the presence of the mecA and femA-SA gene, with the absence of the femA-SE gene. To compensate for the experimental and clinical conditions, we spiked an internal control in the course of DNA extraction. We estimated number of colony-forming units per mL (CFU/mL) of MRSA samples through a standard curve of a serially-diluted reference MRSA strain. We compared the threshold cycle (Ct) value with the microbiologic results of MRSA. Results: We obtained the mecA gene standard curve, which showed the detection limit of the mecA gene to be 100 fg, which corresponds to a copy number of 30. We chose cut-off Ct values of 27.94 (equivalent to $1{\times}10^4$ CFU/mL) and 21.78 (equivalent to $1{\times}10^5$ CFU/mL). The sensitivity and specificity of our assay were 88.9% and 88.9% respectively, when compared with quantitative cultures. Conclusion: Our results were valuable for diagnosing and identifying pathogens involved in VAP. We believe our modified qPCR is an appropriate tool for the rapid diagnosis of clinical pathogens regarding patients in the intensive care unit.

졸-겔 법과 RTA 장비를 이용한 전기 전도성 박막의 연구

金裕振 弘益大學校 科學技術硏究所 2001 科學技術硏究論文集 Vol.12 No.-

Electroconductive antimony-doped tin oxide coating on borosilicate glass was studied. Solutions were prepared at various compositions of alkoxides in ethanol solvents containing small quantities of catalysts and H₂O. Conductive larger was coated by spin casting followed by proper thermal treatment. We could obtain good conductivity from the thin films by tuning the rotation velocity of spinner, the number of layers, the thicknesses of the layers, and the annealing temperature. The lowest sheet resistance achieved was 3600 Ω/sq.

다양한 알코올에 의해 일어나는 수용액내 CTAB 나노구조의 변이에 관한 연구

金裕振 弘益大學校 科學技術硏究所 2005 科學技術硏究論文集 Vol.16 No.-

When the amphiphilic molecules are dissolved in water at the concentration above the critical micelle concentration(CMC), the phenomenon of micelle formation takes place. In this study the structures of the Cetyltrimethyl-ammonium Bromide(CTAB) micelles were investigated using SANS. These have received a considerable academic and industrial interests due to their special properties and various applicabilities. Although it has been well known that micelle of various forms can be formed, there are a lot of problems still not clearly confirmed about the detailed shapes, the size distribution of micelles, and inter-, intra- micelle interactions. For the present structure analysis, "Core and shell model" was introduced, in which the structure is consist of a "core" containing the surfactant hydrocarbon chains, a "shell" containing the charged head groups, a fraction of counter-ions, and water. It was possible to obtain structural information including the location of the alcohols in hydrocarbon core, hydrophilic shell and aqueous solvent media.

LB법을 이용한 TCNQ 박막의 물성 및 전기적 특성

金裕振,李榕洙 弘益大學校 科學技術硏究所 1997 科學技術硏究論文集 Vol.8 No.-

A study on the electrical conduction characteristics of the ultrathin organic films is one of the important factors for the development of molecular electronic devices. The Langmuir- Blodgett(LB) technique has recently been attracted interest as the a method of deposition ultrathin films. We have fabricated N-docosyl-N'-methyl viologen-(TCNQ-)₂(DMVT) anion radical LB film and investigated the physical properties and electrical conduction characteristics. We have measured infrared transmission-reflection spectra. The alkyl chain is found to be well-ordered with the tilt angle of 13˚ to the substrate surface normal and the TCNQ plane is tilted at 76˚ to the surface normal. In ESR spectrum, we confirmed that a half-amplitude linewidth is clearly dependent on both the incident angle and temperature, which indicates conducting species change their electron spin state. The in-plane conductivity of 31 layers is approximately 1.33×10?? S/cm. The ohmic behaviour was observed below 0.6 V and Schottky characteristics was observed in the range of 2.5∼6V, when current-voltage(I-V) characteristics was measured verically.

천연식품 추출 혼합물인 디콜을 이용한 마우스와 랫드에서의 LDL-콜레스테롤, 중성지질 및 체중 감소효과

김지훈,김경범,이유진,정종문 수원대학교 기능성생명소재연구소 2004 자연과학연구논문집 Vol.3 No.1

This study was performed to investigate the effects of natural source extracts, Dechol. The effects of Dechol were investigated an the blood LDL-cholesterol, triglyceride, and body weights in the Sprague Dawley rats, transgenic mice (B6, 129-Ldlr^(tm1Her)), and ICH mice. Hyperlipidemia was induced in SD rats and ICR mice by feeding them with high-fat food. Transgenic mice were tested by feeding them with normal food. All animal experiments were divided into 2 groups. One group was treated in drinking 0.5% Dechol whereas the other group was fed with tap water. Dechol-treated SD rat group could be returned to normal blood LDL-cholesterol level almost in 9 days. ICR mice determination of LDL-cholesterol level showed that the difference after 4 weeks between two groups was 43 mg/dl. Transgenic mice indicated that the blood cholesterol concentration in the subject group was decreasing everyday whereas the one in the control group was increasing, resulting in the difference of 280 mg/dl between two groups just after 20 day-treatment. These results suggest that Dechol may be efficiently used for primary prevention of cardiovascular diseases.

스포츠 관전행동의 결정요인에 관한 연구 : 이용자 특성을 중심으로 Focused on Consumer's Psychological Characteristics

박유진,김재휘 한국스포츠심리학회 2003 한국스포츠심리학회지 Vol.14 No.3

본 연구는 스포츠팬의 심리적 특성이 스포츠 관전행동에 영향을 미친다고 가정하고, 스포츠 관전동기와 스포츠팬 정체성의 다양한 차원들 중 스포츠 관전행동을 결정하는 요인들을 밝히고자 하였다. 질문지 조사를 통한 연구 결과 정체성현출성, 친지관계동기, 대리만족동기, 성별의 네 가지 변인이 스포츠 관전행동을 결정하는 요인으로 밝혀졌다. 또한 관전자 특성에 따라 관저행동에 영향을 미치는 관전동기 및 스포츠팬 정체성 요인에 차이가 있는 것으로 나타났다. 즉, 스포츠팬의 성별 및 소극적 관전수준에 따라 관전행동에 영향을 미치는 관전동기 및 스포츠팬 정체성의 구성요소가 달라졌다. 이러한 연구 결과를 스포츠 마케팅에 활용한다면, 관전자 특성에 따라 보다 세분화된 마케팅 전략을 수립할 수 있을 것이다. Watching sport events represents a predominant form of leisure today. Many people attend sport events and think of themselves as sport fans, So, It is needed to understand psychological natures of sports fans and which of psychological natures lead spectators to stadium. Then we could know what makes more steady and devoted fans. Based on previous researches, we could expect that spectator motivation and fan identity would influence on sporting game attendance of spectator. Also we could expect there were sexual differences in effects of motivation and identity factors. To examine these propositions, we performed survey research. Questionnaire of this study included the scales for sports spectator motivation, fan identity dimensions, sport spectating experiences, and demographic variables. Data of this study were collected from 192 college students. The results of the survey analysis verify relationship motive, identity salience, sex, and vicarious satisfaction determine attendance of sporting games. And there were sexual differences in effect of motivation and identity dimensions. These results meant men and women want different benefits through watching sporting events. Therefore, it is needed diverse marketing strategy according to spectators' natures.

전분의 첨가가 자몽 종자 추출물 DF-100의 항균 활성에 미치는 영향

이유진,김지훈,김영식,정영재,김경범,정종문 수원대학교 기능성생명소재연구소 2003 자연과학연구논문집 Vol.2 No.1

Recent testimonials report grapefruit seed extract DF-100 to be successful in combating a variety of common infectious agents. This study investigated DF-100 for antimicrobial activites at containing 3% corn starch, 3% wheat flour and 0.5~5% starch contents to determine if antimicrobial activity of DF-100 keeps almost constant or is sometimes decreased by the addition of various types of starch. In our study, grapefruit seed extract DF-100 was tested for antibacterial properties against 5 strains. Gram-negative and gram-positive isolates {Escherichia coli, Bacillus cereus, Staphylococcus aureus, Candida albians, and Yeast (be often contaminated strain in confectionery factory)} were introduced into various dilutions of DF-100 (0, 250, 500, 1,000, 2,000, 4,000, 8,000, 16,000 ppm) for determination of antimicrobial activity. The antibacterial effect of the DF-100 appears to suppress the. growth of the 5 strains used for experiment. However, antimicrobial activity of DF-100 was reduced 1/4 to 1/16 when added into foods (such as bread, noodle, and biscuits) containing corn starch, wheat flour, or potato starch. It was evident that the antimicrobial activity DF-100 was decreased by addition of starch.