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On the Production of Axial Prepositions: Linking Figure and Ground in Broca`s Aphasia: A Case Study
( Elisa Zampieri ),( Ludovico Franco ),( Giulia Bencini ),( Francesca Meneghello ) 서강대학교 언어정보연구소 2013 언어와 정보 사회 Vol.19 No.-
Zampieri, Elisa; Franco, Ludovico; Bencini, Giulia, and Meneghello, Francesca(2013), “On the Production of Axial Prepositions: Linking Figure and Ground in Broca`s Aphasia: A Case Study”, Language & Information Society 19. We report the case of a classic agrammatic speaker of Italian in repeating locative Figure/Ground relations via complex prepositions consisting of a locative axial preposition and a simple preposition in light of recent theoretical accounts of prepositions and the existing literature on prepositions in aphasia. The repetition data showed greater rates of omission for simple than axial prepositions, and a trade-off between the inclusion of the Figure and the Axial preposition. Grounds tended to be preserved. These results are unexpected on accounts that simple prepositions are more functional in nature than axial prepositions and argue for an analysis in which axial prepositions can take on a functional grammatical role. To account for the repetition data we propose that the agrammatic processor is unable to fill and retain functional Axial Parts. Hence, it links Figure and Ground through a reduced configuration, mediated by the simple preposition operating as a relational item and not a functional category. The clinical data presented here add to the empirical data set against which to test the validity of finergrained distinctions among prepositions and compare different theoretical views of the status of prepositions.
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( Elisa Friska Romasi ),( Jin Ho Lee ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.12
Biosynthesis of indole-3-acetic acid (IAA) via the indole-3-pyruvic acid pathway involves three kinds of enzymes; aminotransferase encoded by aspC, indole-3-pyruvic acid decarboxylase encoded by ipdC, and indole-3-acetic acid dehydrogenase encoded by iad1. The ipdC from Enterobacter cloacae ATCC 13047, aspC from Escherichia coli, and iad1 from Ustilago maydis were cloned and expressed under the control of the tac and sod promoters in E. coli. According to SDS-PAGE and enzyme activity, IpdC and Iad1 showed good expression under the control of Ptac, whereas AspC was efficiently expressed by Psod originating from Corynebacterium glutamicum. The activities of IpdC, AspC, and Iad1 from the crude extracts of recombinant E. coli Top 10 were 215.6, 5.7, and 272.1 nmol/min/mg-protein, respectively. The recombinant E. coli DH5α expressing IpdC, AspC, and Iad1 produced about 1.1 g/l of IAA and 0.13 g/l of tryptophol (TOL) after 48 h of cultivation in LB medium with 2 g/l tryptophan. To improve IAA production, a tnaA gene mediating indole formation from tryptophan was deleted. As a result, E. coli IAA68 with expression of the three genes produced 1.8 g/l of IAA, which is a 1.6- fold increase compared with wild-type DH5α harboring the same plasmids. Moreover, the complete conversion of tryptophan to IAA was achieved by E. coli IAA68. Finally, E. coli IAA68 produced 3.0 g/l of IAA after 24 h cultivation in LB medium supplemented with 4 g/l of tryptophan.
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Character Combination Dynamics in Web Novels : Focusing on Naver Series and KakaoPage
Elisa Choi,Haneul Kim 한국경영정보학회 2023 한국경영정보학회 학술대회논문집 Vol.2023 No.11
The rise of the internet and smartphones has transformed the web novel industry into a significant cultural force. Platforms like Naver Series and Kakao Page experienced explosive growth, soaring from KRW 10 billion to KRW 640 billion from 2013 to 2020 and surpassing KRW 1 trillion by 2022. With approximately 5.87 million readers[1], this growth is expected to continue, fueled by global exports to Japan and China. In this competitive landscape, platforms enhance user experiences using features like 'keywords' to ensure user loyalty. This study employs text mining to analyze character combinations in top-ranking romance fantasy web novels, aiming to unveil user preferences and correlations, providing valuable insights into the webnovel domain.
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Analysis of Acid Sphingomyelinase Activity in Dried Blood Spots Using Tandem Mass Spectrometry
Elisa Legnini,Joe J. Orsini,Adolf Mühl,Britt Johnson,Angela Dajnoki,Olaf A Bodamer 대한진단검사의학회 2012 Annals of Laboratory Medicine Vol.32 No.5
Background: Niemann Pick disease (NP) is a rare, lysosomal storage disorder due to deficiency of the intra-lysosomal enzyme acid sphingomyelinase (ASM) resulting in intracellular accumulation of sphingomyelin. We evaluated a tandem mass spectrometry (MS/MS)method to analyze ASM activity in dried blood spots (DBS) that may be suitable for laboratory diagnosis of NP including high throughput screening of at-risk populations and potentially for newborn screening. Methods: ASM activity was measured in 3.2 mm punches from DBS. The eluate was incubated with the ASM substrate (N-Hexanoyl-D-erythro-sphingosylphosphorylcholine [C6- sphingomyelin (C29H59N2O6P)]) and an internal standard (N-butyroyl-D-erythro-sphingosine [C4-ceramide (C22H43NO3)]). ASM product and IS were analyzed using MS/MS in multiple reaction monitoring mode for transitions m/z 370.6>264.3 (ASM internal standard) and m/z 398.6>264.3 (ASM product). Results: ASM activities were stable for up to 2 months at or below 4˚C. Position of the punch in the DBS and/or hematocrit of the DBS had a limited effect on ASM activities. Both intraand inter-assay variability were below 10%. There was no carry-over. The median ASM activity in 2,085 newborn infants was 9.5 μmol/h/L (mean 10.6) with a SD of 5.06 μmol/h/L. Six of 2,085 (0.3%) infants were found to have ASM activities below the cut-off of 2.5 μmol/h/L. ASM activities were below the cut-off level in all 10 previously diagnosed cases with NP (range: 0.16 to 2.08 μmol/h/L). Conclusions: This MS/MS method for the measurement of ASM activity in DBS is robust and suitable for laboratory diagnosis of NP. Background: Niemann Pick disease (NP) is a rare, lysosomal storage disorder due to deficiency of the intra-lysosomal enzyme acid sphingomyelinase (ASM) resulting in intracellular accumulation of sphingomyelin. We evaluated a tandem mass spectrometry (MS/MS)method to analyze ASM activity in dried blood spots (DBS) that may be suitable for laboratory diagnosis of NP including high throughput screening of at-risk populations and potentially for newborn screening. Methods: ASM activity was measured in 3.2 mm punches from DBS. The eluate was incubated with the ASM substrate (N-Hexanoyl-D-erythro-sphingosylphosphorylcholine [C6- sphingomyelin (C29H59N2O6P)]) and an internal standard (N-butyroyl-D-erythro-sphingosine [C4-ceramide (C22H43NO3)]). ASM product and IS were analyzed using MS/MS in multiple reaction monitoring mode for transitions m/z 370.6>264.3 (ASM internal standard) and m/z 398.6>264.3 (ASM product). Results: ASM activities were stable for up to 2 months at or below 4˚C. Position of the punch in the DBS and/or hematocrit of the DBS had a limited effect on ASM activities. Both intraand inter-assay variability were below 10%. There was no carry-over. The median ASM activity in 2,085 newborn infants was 9.5 μmol/h/L (mean 10.6) with a SD of 5.06 μmol/h/L. Six of 2,085 (0.3%) infants were found to have ASM activities below the cut-off of 2.5 μmol/h/L. ASM activities were below the cut-off level in all 10 previously diagnosed cases with NP (range: 0.16 to 2.08 μmol/h/L). Conclusions: This MS/MS method for the measurement of ASM activity in DBS is robust and suitable for laboratory diagnosis of NP.
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Stainless Steel Porous Substrates Produced by Tape Casting
Elisa Mercadelli,Angela Gondolini,Paola Pinasco,Alessandra Sanson 대한금속·재료학회 2017 METALS AND MATERIALS International Vol.23 No.1
In this work the technological issues related to the production of tape cast large-area porous stainless steel supports for Solid Oxide Fuel Cells (SOFC) applications were carefully investigated. The slurry formulation was optimized in terms of amount and nature of the organic components needed: rice starch and polymethyl metacrylate were found to be, respectively, the most suitable pore former and binder because easily eliminated during the thermal treatment in reducing atmosphere. The compatibility of the binder system chosen with the most widely used solvents for screen printing inks was also evaluated. Finally the influence of the sintering temperature and of the refractory supports to be used during the thermal treatments onto the production of porous stainless steel supports was discussed. The whole process optimization allows to produce flat, crack-free metallic substrate 900-1000 μm thick, dimensions up to 5×5 cm and with a tailored porosity of 40% suitable for SOFCs application.
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Elisa Eugenia Córdoba,Martín Carlos Abba,Ezequiel Lacunza,Eduardo Fernández,Alba Mabel Güerci 대한암학회 2016 Cancer Research and Treatment Vol.48 No.3
Purpose Reactive oxygen species (ROS) are generated as an indirect product of radiation therapy (RT). Genetic variation in genes related to ROS metabolism may influence the level of RT-induced adverse effects. We evaluated the potential association of single nucleotide polymorphism (SNP)–related response to radiotherapy injury in breast cancer patients undergoing RT. Materials and Methods Eighty patients receiving conventional RT were included. Acute effects were evaluated according to the Radiation Therapy Oncology Group (RTOG) scores. DNA was extracted from blood and buccal swab samples. SNPs were genotyped for GSTP1, GSTA1, SOD2, and NOS3 genes by polymerase chain reaction–based restriction fragment length polymorphism. Univariate analysis (odds ratios [ORs] and 95% confidence interval [CI]) and principal component analysis were used for correlation of SNPs and factors related to risk of developing ! grade 2 acute effects. Results Sixty-five patients (81.2%) showed side effects, 32 (40%) presented moderate to severe acute skin toxicity, and 33 (41.2%) manifested minimal acute skin reactions by the end of treatment. In both univariate and multivariate analyses, nominally significant associations were found among body mass index (OR, 3.14; 95% CI, 8.5338 to 1.1274; p=0.022), breast size (OR, 5.11; 95% CI, 17.04 to 1.54; p=0.004), and grade ! 2 acute radiation skin toxicity. A significant association was also observed between NOS3 G894T polymorphism (OR, 9.8; 95% CI, 211.6 to 0.45; p=0.041) and grade ! 2 acute radiation skin toxicity in patients with neo-adjuvant chemotherapy treatment. Conclusion The analysis of the factors involved in individual radiosensitivity contributed to the understanding of the mechanisms underlying this trait.
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Prevalence of Non-erosive Esophageal Phenotypes in Children: A European Multicenter Study
Elisa Blasi,Ettore Stefanelli,Renato Tambucci,Silvia Salvatore,Paola De Angelis,Paolo Quitadamo,Claudia Pacchiarotti,Giovanni Di Nardo,Fanj Crocco,Valentina Giorgio,Nicoletta Staropoli,Simona Sestito 대한소화기 기능성질환∙운동학회 2023 Journal of Neurogastroenterology and Motility (JNM Vol.29 No.2
Background/AimsSince available data on pediatric non-erosive esophageal phenotypes (NEEPs) are scant, we investigated their prevalence and the phenotype-dependent treatment response in these children. MethodsOver a 5-year period, children with negative upper endoscopy, who underwent esophageal pH-impedance (off-therapy) for persisting symptoms not responsive to proton pump inhibitor (PPI)-treatment, were recruited. Based on the results of acid reflux index (RI) and symptom association probability (SAP), patients were categorized into: (1) abnormal RI (non-erosive reflux disease [NERD]), (2) normal RI and abnormal SAP (reflux hypersensitivity [RH]), (3) normal RI and normal SAP (functional heartburn [FH]), and (4) normal RI and not-reliable SAP (normal-RI-not otherwise-specified [normal-RI-NOS]). For each subgroup, treatment response was evaluated. ResultsOut of 2333 children who underwent esophageal pH-impedance, 68 cases, including 18 NERD, 14 RH, 26 FH, and 10 normal-RI-NOS were identified as fulfilling the inclusion criteria and were analyzed. Considering symptoms before endoscopy, chest pain was more reported in NERD than in other cases (6/18 vs 5/50, P = 0.031). At long-term follow-up of 23 patients (8 NERD, 8 FH, 2 RH, and 5 normal-RI-NOS): 17 were on PPIs and 2 combined alginate, 1 (FH) was on benzodiazepine + anticholinergic, 1 (normal-RI-NOS) on citalopram, and 3 had no therapy. A complete symptom-resolution was observed in 5/8 NERD, in 2/8 FH, and in 2/5 normal-RI-NOS. ConclusionsFH may be the most common pediatric NEEP. At long-term follow-up, there was a trend toward a more frequent complete symptom resolution with PPI-therapy in NERD patients while other groups did not benefit from extended acid-suppressive-treatment.
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Enhanced oxidative stability of meat by including tannin-rich leaves of woody plants in goat diet
Elisa Mariana Garcia,Agustin Lopez,Maria Zimerman,Olegario Hernandez,Jose Ignacio Arroquy,Monica Azucena Nazareno 아세아·태평양축산학회 2019 Animal Bioscience Vol.32 No.9
Objective: The aim of this study was to evaluate the effect of dietary incorporation of tannin-rich woody species on meat oxidative stability, carcass traits and meat quality in goats. Methods: Two tannin-rich species were tested using a three-treatments feeding trial, where treatments consisted of: Larrea divaricata and Acacia aroma both at 12.5% in dry matter basis of the diet and a control diet (alfalfa hay). All feeding diets were iso-protein and iso-energy. Carcass conformation, carcass compactness, carcass fatness and subcutaneous fat deposition were evaluated. Intake, liveweigh, Longissimus thoracis et lumborum muscles of goats were analyzed in order to evaluate quality parameters such as pH value, instrumental color evaluation, water holding capacity, total phenolic content, antioxidant activity, meat oxidative stability and fatty acid profiles in meat. Results: Feed intake, liveweight gain, carcass, and meat traits did not differ among treatments. Changes in meat lipid profile among treatments were observed for oleic and elaidic acid contents. Meat total phenolic content and antioxidant activity did not differ among treatments; although, meat oxidative status after storage at room temperature, as well as under refrigerated and frozen conditions were different between control and both supplemented groups. Conclusion: The inclusion of Acacia aroma and Larrea divaricata leaves in goat diet enhanced meat oxidative stability. Modulation of the ruminal biohydrogenation of fatty acids produced by condensed tannins of these plant species need to be further investigated.
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