http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Lee, Young Ju,Lee, Doyeon,Shin, So Min,Lee, Jin Sun,Chun, Hyun Soo,Quan, Fu-Shi,Shin, Jae Ho,Lee, Gi-Ja ELSEVIER SCIENCE B.V.; AMSTERDAM 2017 JOURNAL OF FUNCTIONAL FOODS Vol.33 No.-
<P><B>Abstract</B></P> <P>In this study, we investigated the cardioprotective effects of fermented garlic extract (FGE) against ischemia-reperfusion (IR) injury in H9c2 cells and myocardium. FGE used in this study is rich in nitrite (1.42mg/ml of FGE) and reduced glutathione (GSH, 3.23mg/ml of FGE). We compared the effectiveness of FGE against HR–induced oxidative stress with that of nitrite, GSH, and a mixture of nitrite and GSH (nitrite+GSH). In addition, we utilized fluorinated xerogel-derived oxygen microsensors to measure the changes in oxygen level (<I>p</I>O<SUB>2</SUB>) of the myocardium during myocardial IR by FGE. As a result, the <I>p</I>O<SUB>2</SUB> in the FGE group restored to pre-ischemic level (75.1±36.9%) at reperfusion period; however, that of the control group did not increase throughout reperfusion period (2.0±1.7%, <I>p</I> <0.05). Therefore, pre-treatment with FGE might be helpful as a therapeutic strategy for preventing H9c2 cells and myocardium from IR injury.</P> <P><B>Highlights</B></P> <P> <UL> <LI> We investigated the cardioprotective effect of FGE against myocardial IR injury. </LI> <LI> FGE was rich in natural nitrite (1.422mg/ml) and glutathione (3.23mg/ml). </LI> <LI> Pre-treatment with FGE could protect H9c2 cells from HR-induced injury. </LI> <LI> pre-treatment with FGE could promote reoxygenation in post-ischemic myocardiaum. </LI> <LI> FGE might be useful in preventing myocardial IR injury. </LI> </UL> </P>
Yu, Namhee,Yong, Seunghui,Kim, Hong Kwan,Choi, Yoon‐,La,Jung, Yeonjoo,Kim, Doyeon,Seo, Jihae,Lee, Ye Eun,Baek, Daehyun,Lee, Jinseon,Lee, Seungjae,Lee, Jong Eun,Kim, Jaesang,Kim, Jhingook,Lee, Sa John Wiley and Sons Inc. 2019 MOLECULAR ONCOLOGY Vol.13 No.6
<P>The roles of miRNAs in lung cancer have not yet been explored systematically at the genome scale despite their important regulatory functions. Here, we report an integrative analysis of miRNA and mRNA sequencing data for matched tumor–normal samples from 109 Korean female patients with non‐small‐cell lung adenocarcinoma (LUAD). We produced miRNA sequencing (miRNA‐Seq) and RNA‐Seq data for 48 patients and RNA‐Seq data for 61 additional patients. Subsequent differential expression analysis with stringent criteria yielded 44 miRNAs and 2322 genes. Integrative gene set analysis of the differentially expressed miRNAs and genes using miRNA–target information revealed several regulatory processes related to the cell cycle that were targeted by tumor suppressor miRNAs (TSmiR). We performed colony formation assays in A549 and NCI‐H460 cell lines to test the tumor‐suppressive activity of downregulated miRNAs in cancer and identified 7 novel TSmiRs (miR‐144‐5p, miR‐218‐1‐3p, miR‐223‐3p, miR‐27a‐5p, miR‐30a‐3p, miR‐30c‐2‐3p, miR‐338‐5p). Two miRNAs, miR‐30a‐3p and miR‐30c‐2‐3p, showed differential survival characteristics in the Tumor Cancer Genome Atlas (TCGA) LUAD patient cohort indicating their prognostic value. Finally, we identified a network cluster of miRNAs and target genes that could be responsible for cell cycle regulation. Our study not only provides a dataset of miRNA as well as mRNA sequencing from the matched tumor–normal samples, but also reports several novel TSmiRs that could potentially be developed into prognostic biomarkers or therapeutic RNA drugs.</P>
The Effects of Preoperative Aspirin on Coronary Artery Bypass Surgery: a Systematic Meta-Analysis
Doyeon Hwang,Joo Myung Lee,Tae-Min Rhee,Young-Chan Kim,Jiesuck Park,Jonghanne Park,Chul Ahn,Young Bin Song,Joo-Yong Hahn,Ki-Bong Kim,Young-Tak Lee,Bon-Kwon Koo 대한심장학회 2019 Korean Circulation Journal Vol.49 No.6
Background and ObjectivesAspirin plays an important role in the maintenance of graft patency and the prevention of thrombotic event after coronary artery bypass graft surgery (CABG). However, the use of preoperative aspirin is still under debate due to the risk of bleeding. MethodsFrom PubMed, EMBASE, and Cochrane Central Register of Controlled Trials, data were extracted by 2 independent reviewers. Meta-analysis using random effect model was performed. ResultsWe performed a systemic meta-analysis of 17 studies (12 randomized controlled studies and 5 non-randomized registries) which compared clinical outcomes of 9,101 patients who underwent CABG with or without preoperative aspirin administration. Preoperative aspirin increased chest tube drainage (weighted mean difference 177.4 mL, 95% confidence interval [CI], 41.3–313.4; p=0.011). However, the risk of re-operation for bleeding was not different between the preoperative aspirin group and the control group (3.2% vs. 2.4%; odds ratio [OR], 1.23; 95% CI, 0.94–1.60; p=0.102). There was no difference in the rates of all-cause mortality (1.6% vs. 1.5%; OR, 0.98; 95% CI, 0.64–1.49; p=0.920) and myocardial infarction (MI) (8.7% vs. 10.4%; OR, 0.83; 95% CI, 0.66–1.04; p=0.102) between patients with and without preoperative aspirin administration. ConclusionsAlthough aspirin increased the amount of chest tube drainage, it was not associated with increased risk of re-operation for bleeding. In addition, the risks of early postoperative all-cause mortality and MI were not reduced by using preoperative aspirin.
Development of bioinformatics and multi-omics analyses in organoids
Doyeon Ha,JungHo Kong,Donghyo Kim,Kwanghwan Lee,Juhun Lee,Minhyuk Park,Hyunsoo Ahn,Youngchul Oh,Sanguk Kim 생화학분자생물학회 2023 BMB Reports Vol.56 No.1
Pre-clinical models are critical in gaining mechanistic and biologicalinsights into disease progression. Recently, patient-derivedorganoid models have been developed to facilitate our understandingof disease development and to improve the discoveryof therapeutic options by faithfully recapitulating in vivo tissuesor organs. As technological developments of organoid modelsare rapidly growing, computational methods are gaining attentionin organoid researchers to improve the ability to systematicallyanalyze experimental results. In this review, we summarize therecent advances in organoid models to recapitulate human diseasesand computational advancements to analyze experimentalresults from organoids.
Lee, Geumbee,Kim, Daeil,Kim, Doyeon,Oh, Sooyeoun,Yun, Junyeong,Kim, Jihyun,Lee, Sang-Soo,Ha, Jeong Sook The Royal Society of Chemistry 2015 ENERGY AND ENVIRONMENTAL SCIENCE Vol.8 No.6
<P>In this study, we report the fabrication of a stretchable and patchable array of micro-supercapacitors (MSCs) using a gel-type electrolyte of poly(methyl methacrylate)–propylene carbonate–lithium perchlorate. As electrodes, a layer-by-layer-assembled thin film of multi-walled carbon nanotubes with a top layer of Mn<SUB>3</SUB>O<SUB>4</SUB> nanoparticles was used. The fabricated MSC maintained over 85% of its performance for 2 weeks in ambient air without encapsulation owing to the use of a non-aqueous solvent based gel electrolyte. Dry-transferred MSC arrays on a specially designed stretchable polymer substrate exhibited stable electrochemical performance under various deformations, including bending, twisting, both uniaxial and biaxial stretching up to 50%, and winding around the curved substrate. Furthermore, the encapsulated MSC array with a thin polymer film directly attached to skin maintained its electrochemical performance under repeated body movement, cycles of attachment–detachment, and even in water. This study clearly demonstrates a stretchable and patchable MSC array for practical use as an energy storage device that can be attached to the body for electronic function, even under wet conditions.</P> <P>Graphic Abstract</P><P>We report on air stable planar micro-supercapacitors (MSCs) using a non-aqueous solvent based gel electrolyte and stable performance of encapsulated MSC array under stretching and exposure to water. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c5ee00670h'> </P>
김도연(Doyeon Kim),이슬아(Seulah Lee),이현진(Hyun Jin Lee),박경호(Kyoung Ho Park) 대한두개저학회 2020 대한두개저학회지 Vol.15 No.1
Squamous cell carcinoma (SCC) of the external auditory canal is uncommon malignancy with an annual incidence of one per one million inhabitants. Common tumor progression site are periauricular soft tissues, the parotid gland, temporomandibular joint and mastoid. SCC of the external auditory canal is an aggressive disease with the prognosis dependent on the stage of disease and the primary treatment. Early signs of this disease are ear pain and discharge. The treatment of choice is aggressive surgery with postoperative radiation. A 73-year-old woman with the left ear discharge and mass lesion visited our outpatient clinic. A 1 cm diameter mass was observed at the entrance of the left external auditory canal. Biopsy revealed a squamous cell carcinoma. We performed total auriculectomy, subtotal petrosectomy and superficial parotidectomy. We reviewed this case with reference to relevant publications in the medical literature.
국가현안 대응형 데이터 기반 정부출연연구기관의 융합R&D전략 기획 프레임워크에 대한 연구: 고령화를 중심으로
이도연(Doyeon Lee),김근환(Keunhwan Kim) 한국기술혁신학회 2020 한국기술혁신학회 학술대회 발표논문집 Vol.2020 No.11
초고령화 사회로 진입하려는 우리나라는 상당한 경제적·사회적 문제가 발생할 것이 예상되고 있다. 국가의 난제인 고령화 문제의 충격에 대비하기 위한 연구개발(R&D)에 정부 출연연구기관의 역할과 책임(Role and Responsibility)이 더욱 강화되고 있으며, 국가·사회적 요구가 증대되고 있는 가운데, 고령화라는 국가적 난제를 해결하기 위해 정부출연연구기관을 중심으로 수평적·수직적 협력을 통해 융합연구를 더욱 확대할 전략수립의 필요성이 더욱 높아지고 있다. 국가연구개발 투자의 효율성을 높이기 위해 전략기획을 수립하는 단계에서 객관성 및 합리성을 확보할 수 있는 증거기반의 정보 제공이 필수조건이다. 본 연구에서는 국가 난제인 고령화 이슈와 관련한 출연연의 융합R&D전략 수립에 필요한 중점 연구영역(분류체계) 도출과 연구영역별 R&D 수행주체 및 지원현황 정보를 위해 국가 R&D 데이터를 기반으로 제공하는 프레임워크를 제안하였다. 국가과학기술지식정보서비스(NTIS)에서 2017-20년 기간 동안 고령화와 관련된 2,534건의 R&D과제를 대상으로 Scopus의 All Science Journal Classification(ASJC)을 기반으로 분류하고, 이를 기반으로 동시발생현(co-occurrence) 분석결과를 통해 클러스터링을 수행하였다. 본 연구를 통해 증거기반 R&D전략을 수립하는 과정에서 가장 기초가 되는 정보를 제공하여 이해관계자들의 적극적인 협력을 유도하여 정책의 신뢰성을 제고시킬 수 있는 가능성을 확인하였다. Korea, enters a super-aged society by 2026, is expected to encounter considerable economic and social problems. Government-funded research institutes (GFRIs) have been required for their role and responsibility in R&D to prepare for the impact of the nation"s aging problem. In order to solve the national challenge of aging, it is required for GFRIs to expand convergence research through horizontal and vertical cooperation with other research organizations. Thus, it is essential to provide evidence-based information that can secure objectivity and rationality at the early stage of strategic planning, thereby increasing the efficiency of national R&D investment, In this study, we propose a framework to derive key research areas, and then research organizations and investment trend per key research areas, which needed to discuss among stakeholder. After nationally-funded 2,534 R&D projects related to aging (2017-20) were collected from The National Science and Technology Information Service (NTIS), Scopus" All Science Journal Classification (ASJC) was designated, and then conducted cluster analysis based on the co-occurrence. The results showed that the possibility of enhancing the credibility of the policy was confirmed by providing evidence-based information in the process of establishing the R&D strategy.