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옥수수 태생돌연변이체-1 및 정상체의 호분층 (糊紛層) 유출물과 ABA 의 결합
배동규 ( Dong Gyu Bai ),문정선 ( Jung Sun Moon ),( J. D. Smith ) 한국식물학회 1994 Journal of Plant Biology Vol.37 No.2
Aleuroue layers of normal and vp1 mutant maize kernels were extracted and centrifuged at 100,000 g to yield a cytosol fraction. Binding of [^3H]ABA cis, trans (+)ABA to a soluble macromolecular components present in the cytosol was demonstrated by Sephadex chromatography and non-denaturing PAGE. The binding component was of high molecular weight and seems to be an aggregate of proteins. A rapid DEAE-cellulose filter method for assaying bound [^3H]ABA to a soluble protein was adapted. Biding assays were performed with cytosol that had been preheated or incubated with several enzymes, indicating that heat and protease treatments disrupted the binding. This suggested that binding occurred to proteins. Some properties of the ABA binding proteins were described. The [^3H]ABA binding were reduced dramatically when unlabeled ABA was added as a competitor, Suggesting a specific binding of [^3H]ABA. Gel filtration profiles and autoradiogram of [^3H]ABA binding showed no difference in the binding components of Vp1 and vp1/vp1 mutant cytosol, indicating that Vp1 protein is not a sole ABA binding protein.
The Effect of Pre-treatment on Physicochemical Properties of Colocasia esculenta (L.) Schott Stem
Dong Hyeon Park,Sang Yoon Lee,Jiseon Lee,Soojin Kim,Jae-Hee Seo,Jung Gyu Lee,Hee Jeong Choi,Jing Jing Bai,Ha Yoon Kim,Mi-Jung Choi 한국산업식품공학회 2016 학술대회 및 심포지엄 Vol.2016 No.04
There are a lot of types of wild vegetables such as Colocasia esculenta (L.) Schott stem in Korea. However, the consumption of these wild vegetables is restricted because their storage decreased dramatically after harvest. To maintain original quality of vegetables, pre-treatments such as blanching and drying are important. But conditions for these treatments were still not optimized for many vegetables including Colocasia esculenta (L.) Schott stem. Thus, the objective of this study was to set up an optimal pre-treatment method for freezing storage. Colocasia esculenta (L.) Schott stems were peeled and cut equally (10 cm) for sample preparation. Dried samples (D) were dried at 90℃ for 3 h. Blanched samples (B) were blanched in hot water at 100℃ for 2 min. Blanched and dried samples (BD) were blanched and dried as same protocol. Physicochemical properties were analyzed to evaluate the quality including texture, moisture content, total color difference and viable cell count. Raw sample had 6.85 kg/cm 3 of hardness and 78.75 of chewiness whereas B was 6.83 kg/cm 3 of hardness and 7.8 of chewiness. B had the similar value compared to raw samples. Moisture content of raw sample was 94.4% and that of B was 94.1%, though there were not any significant differences between them. ΔE value of B showed lower value than those of the others. Viable cell counts and total coliforms were not detected after treatment, while raw sample had 5.39 log CFU/g of viable cell count without total coliform. Therefore, pre-treatments are essential for microbial safety of samples. All results considered, it is supposed that blanching is the optimal pre-treatment to sustain its original quality of Colocasia esculenta (L.) Schott stems before freezing.
Analysis of lawsuit cases in the Department of Surgery in Korea
Ji Yun Jung,김소윤,Dong Gyu Kim,Choong Bai Kim,Kyong-Choun Chi,강원경,Won Lee 대한외과학회 2018 Annals of Surgical Treatment and Research(ASRT) Vol.94 No.3
Purpose: The aim of this study is to prepare medical staff in order to prevent medical malpractice litigation through analysis of litigation cases related to the department of surgery in Korea. Methods: A total of 94 litigation cases related to the department of surgery, where a certain amount of payment was ordered to the defendant between 2005 through 2010, were analyzed. We examined time of occurrence, amount claimed and awarded in damages, plaintiff claims, and court opinion. Results: An average of 3.2 years was spent from the date of the incident occurring to the end of the litigation procedures. The average amount awarded in judgments for damages was 59,708,983 ± 67,307,264 (range, 1,700,000–365,201,482) Korean won. Cases were found involving the following opinion of the court: violation of duty of care (49 cases), violation of informed consent (7 cases), violation of duty of care and informed consent (5 cases), and settlement, reconciliation, and others (32 cases). By analyzing defendants’ negligence in court opinions, diagnosis (30.8%) was the most common, followed by post-operation management (27.7%). Conclusion: Physicians have to conduct treatment and surgery based on exact diagnosis and be careful to observe patients’ conditions and symptoms after surgery. It is essential to identify the current status and characteristics of medical litigation for reducing further litigation and improving patient safety. In order to create a safe medical environment, national efforts should be made not only by individuals but also at the national level.
High Frequency Shoot Regeneration from leaf Explants of Cucumber
Seo, Seung-Hee,Bai, Dong-Gyu,Park, Hyeon-Yong The Korean Society of Plant Biotechnology 2000 JOURNAL OF PLANT BIOTECHNOLOGY Vol.2 No.1
Leaf explants of the cucumber (Cucumis sativus L.) were cultured on Murashige and Skoog's (MS) medium supplemented with various concentrations of $\alpha$-naphthalene acetic acid (NAA) and 6-benzylaminopurine (BAP). Direct shoot orgnogenesis as well as callus formation with somatic embryos and multiple shoots was observed from leaf explants of cvs. Shinhukjinju and Chungjang. The highest frequency of shoot formation 80% was observed on MS medium supplemented with NAA/BAP (5.0 ${\mu}{\textrm}{m}$/2.5 ${\mu}{\textrm}{m}$), with explants forming 3-7 shoots. Shoots formation occured within 3 to 4 weeks. Only one subculture of calli was required for plant regeneration on normal growth regulator-free medium. Plantlets transferred to soil developed into plants of normal appearance, which flowered and set fruits.
The Effect of Sugar as a Cryoprotectant on the Physicochemical Properties of Frozen Soybean Sprouts
Jae-Hee Seo,Dong-Hyun Park,Hee Jeong Choi,Jingjing Bai,Jiseon Lee,Soojin Kim,Jung Gyu Lee,SangYoon Lee,Eun-Young Ko,Mi-Jung Choi 한국산업식품공학회 2016 학술대회 및 심포지엄 Vol.2016 No.10
Cryoprotectant is a substance used to protect biological tissue from freezing injury. However, there was few research paper on application of cryoprotectant in food stuff although its benefits was approved from the biological cell tissues. The objective of this study was to investigate the effect of the sugar addition as a cryoprotectant on the properties of frozen soybean sprouts. Before freezing process, the samples were blanched at 100°C for 1 min to observe the influence of blanching treatment. The blanched or non-blanched soybean sprouts was immersed in sugar solution as cryoprotectant, and continuously, the samples were frozen at -18°C for 24 h. Their physicochemical properties such as drip loss, hardness, color and cellular tissue were analyzed after thawing in running water. In our study, the drip loss of blanched sample without sugar was 43%, and comparatively, blanched one with sugar was 20% which was the lowest value among all samples. There was no significant difference of hardness between sample with sugar and without sugar. From our results, it was supposed that sugar can protect the soybean sprouts during freezing process regardless blanching process.