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김은경 ( Eun-gyeong Kim ),김민경 ( Min-kyung Kim ),권현애 ( Hyun-ae Kwon ),윤도경 ( Do-kyung Youn ),구정헌 ( Jeong-heon Koo ),박소연 ( So-yeon Park ),이희근 ( Hui-geun Lee ),조명희 ( Myeong-hui Jo ),하도윤 ( Do-yun Hah ),김철호 ( 한국동물위생학회(구 한국가축위생학회) 2018 韓國家畜衛生學會誌 Vol.41 No.3
Salmonella Enteritidis (S. Enteritidis) are found in animals, humans, and environment. In addition, S. Enteritidis draws attention to the public health concerns due to carriage of antibiotic resistance traits. For these reasons, the prevalence and antibiotic resistance patterns of S. Enteritidis are significant issues with regard to public health. To address this issues, a total of 24 strains of S. Enteritidis from 164 samples collected from several slaughterhouses in Gyeong-Nam province in order for antibiotic resistance profiles. Subsequently, we characterized the genotyping by random amplification polymorphic DNA (RAPD)-PCR. As a result, very high level of resistance to protein synthesis inhibition antibiotics and most isolates were susceptible to others. Six random primers were used for RAPD-PCR to reveal genotypes of S. Enteritidis isolates. One of the primer, P1245, generated 147 distinct RAPD-PCR fragments ranging from 400∼3000 bp. The number of RAPD-PCR products ranged from 4 to 8 for this primer. The RAPD-PCR fragments could be placed these strains into 3 subgroups and 2 classes by UPGMA cluster analysis. Interestingly, several S. Enteritidis that isolated from different slaughterhouses showed same genotype. These results showed only limited genetic variation among the isolates, those were grouped into a few different patterns of antibiotic resistance.
헤모글로빈의 Microencapsulation에 의한 인공적혈구의 산소전달과 용혈도에 관한 연구
나도춘,김기범,김성종,정경락 전북대학교 공학연구원 ( 구 전북대학교 공업기술연구소 ) 2003 工學硏究 Vol.34 No.-
In this study, we tried to improve oxygen transfer rate and reduce pressure drop on intravascular lung assist device with microencapsulation of red blood cells, because microcapsulation of red cells reduce blood hemolysis. Hemoglobin was purified from outdated human red blood cells used. Phospholipid was purified from egg yolk. The hemosome was prepared by encapsulation of hemoglobin with phospholipid multilayer, using rotary vacuum evaporator. The function of hemosome and hemosome/blood mixed solution was tested by measuring oxygen dissociation curve using blood gas analyzer. Also we were measured about hemolysis of hemosome, hemosome/blood mixed solution and blood by absorptivity method. As a results, the oxygen dissociation curve of hemosome and hemosome/blood mixed solution showed the same figure as the curve of normal red blood cells. Also, the hemolysis of hemosome and hemosome/blood mixed solution was measured less than normal red blood cell. Therefore, the oxygen transfer rate showed high use of hemosome, and hemosome/blood mixed solution rather than normal blood. Because microen- capsulation of normal red blood cells prevent hemolysis of normal red blood cells.
프로젝트 접근법(project approach)이 유아의 대본지식 증진에 미치는 효과
임상도,문경자 한국아동교육학회 2003 아동교육 Vol.12 No.2
There were many studies that examine the effects of project approach on the children's sociality, creativity, expression, vocabulary, problem-solving and so forth, but no empirical studies has been made that illumination the effects of project approach on the children's script knowledge. This study aims to examine the effects of project approach on the script knowledge of kindergarteners. The subjects were 20 children sampled randomly from 2 kindergartens in C city, 10 children were assigned to experimental group receiving program in project approach and the other 10 were assigned to control group receiving no particular program. The experimental design was pre-post control-experimental design. The experiment was conducted for 6 weeks in April and May 2003. To test the effects of project approach, questions about dressing, eating, shopping, going to birth party and going to hospital were used. These questions are supposed to represent script knowledge. The data were analyzed by SPSS/win. The conclusion obtained from the results were as follows. First, the project approach was effective in improving script knowledge about dressing. Second, the project approach was effective in improving script knowledge about eating. Third, the project approach was effective in improving script knowledge about shopping. Fourth, the project approach was effective in improving script knowledge about going to birth party. Fifth, the project approach was effective in improving script knowledge about going to hospital. Overall, project approach was effective in improving the script knowledge.
李乙熙,崔相道,吳錫斗,高鏡畢 진주산업대학교 농업기술연구소 1991 農業技術硏究所報 Vol.4 No.-
아미노산과 5炭糖을 121℃에서 15분간 열처리하여 Maillard반응의 發色强度를 비교한 결과는 다음과 같다. 1. 아미노산은 發色强度가 높은것, 중간것, 낮은것으로 대별되었다. 2. 糖은 L(+)arabinose, D(-)-ribose, D(+)-xylose 순으로 發色强度가 높게 나타났다. 3. pH차이에 따른 영향은 反應液의 pH가 높아질수록 反應性은 增加되었다. 4. D(+)-xylose, D(-)-ribose 및 L(+)-arabinose의 농도가 높아질 수록 반응성은 높게 나타났다. Common amino acids and pentoses have been ranked acording to the intensity of Maillard browning formed when heated in an autoclave at 121℃ for 15 min. The results obtained as follows; 1. The amino compounds have been groupeed into high, intermediate and low browning producing groups. 2. The color intensity of pentoses tested was xylose>ribose>arabinose. 3. As the pH values of reaction solutions increased, the color intensity of melanoidins. 4. As the concentrations of xylose, ribose and arabinose increased, the color intensities of melanoidins.
김기범,나도춘,김성종,정경락 전북대학교 공학연구원 ( 구 전북대학교 공업기술연구소 ) 2003 工學硏究 Vol.34 No.-
We intended to develope an vibrating intravascular lung assist device(VIVLAD) in order to exchange of efficiently gas transfer of oxygen and carbon dioxide exchange requirements for end-stage acute respiratory distress syndrome (ARDS) patients. However, the clinical study, concluded that more gas exchange was needed for intravascular oxygenation to be clinically effective in ARDS treatment. In this study, we tried to enhance gas exchange on the VIVLAD by using microencapsulation of hemoglobin, as the microencapsulation of hemoglobin reduces blood hemolysis, and perfluorocarbon emulsion(PFC emulsion). As a result, it was shown that the oxygen transfer of hemosome and blood/hemosome mixed solution were higher than that of whole blood. Also, it showed that the carbon dioxide transfer of whole blood/PFC emulsion mixed solution was higher than that of others. Therefore, we determined that hemosome and PFC emulsion could increase oxygen transfer and carbon dioxide transfer, respectively.
Do Gyeong Lee,Jin Sung Choi,Seung Woo Yeon,En Ji Cui,Hee Jung Park,Jong Su Yoo,In Sik Chung,Nam In Baek 한국응용생명화학회 2010 Journal of Applied Biological Chemistry (J. Appl. Vol.53 No.3
One secoiridoid glycoside was isolated from the EtOAc-soluble fraction of flowers of Osmanthus fragrans var. aurantiacus by repeated silica gel, octadecyl silica gel (ODS) and Sephadex LH-20 column chromatographies. It was identified as (8E)-ligstroside ba