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Zhu Qinchao,Zhou Xuhao,Zhang Yanan,Ye Di,Yu Kang,Cao Wangbei,Zhang Liwen,Zheng Houwei,Sun Ziyang,Guo Chengchen,Hong Xiaoqian,Zhu Yang,Zhang Yajun,Xiao Ying,Valencak Teresa G.,Ren Tanchen,Ren Daxi 한국생체재료학회 2023 생체재료학회지 Vol.27 No.00
Background : Post-traumatic massive hemorrhage demands immediately available first-aid supplies with reduced operation time and good surgical compliance. In-situ crosslinking gels that are flexibly adapting to the wound shape have a promising potential, but it is still hard to achieve fast gelation, on-demand adhesion, and wide feasibility at the same time. Methods : A white-light crosslinkable natural milk-derived casein hydrogel bioadhesive is presented for the first time. Benefiting from abundant tyrosine residues, casein hydrogel bioadhesive was synthesized by forming di-tyrosine bonds under white light with a ruthenium-based catalyst. We firstly optimized the concentration of proteins and initiators to achieve faster gelation and higher mechanical strength. Then, we examined the degradation, cytotoxicity, tissue adhesion, hemostasis, and wound healing ability of the casein hydrogels to study their potential to be used as bioadhesives. Result : Rapid gelation of casein hydrogel is initiated with an outdoor flashlight, a cellphone flashlight, or an endoscopy lamp, which facilitates its usage during first-aid and minimally invasive operations. The rapid gelation enables 3D printing of the casein hydrogel and excellent hemostasis even during liver hemorrhage due to section injury. The covalent binding between casein and tissue enables robust adhesion which can withstand more than 180 mmHg blood pressure. Moreover, the casein-based hydrogel can facilitate post-traumatic wound healing caused by trauma due to its biocompatibility. Conclusion : Casein-based bioadhesives developed in this study pave a way for broad and practical application in emergency wound management.
Research on Crash Recovery Strategy for Distribution Network Containing DG
Di Bai,Tieyan Zhang,Zheng Yang 보안공학연구지원센터 2015 International Journal of Grid and Distributed Comp Vol.8 No.3
In order to fully utilize DG and improve system reliability, the author puts forward the rash recovery strategy for distribution network with DG. It adopts the heuristic method by taking DG as the source point of research for distribution network power recovery. Based on simplifying the network through improving branch method with the consideration of purposes of network damage to minimum extent and switch action time, it conducts reconstruction after the crash. It optimizes the power supply recovery strategy according to the load transfer condition after the crash in order to minimize the damage to distribution network caused by blackout. It conducts example test at IEEE33 node and IEEE69 node to verify the validity of this scheme.
MLSE-Net: Multi-level Semantic Enriched Network for Medical Image Segmentation
Di Gai,Heng Luo,Jing He, Baogang Xie,Pengxiang Su,Zheng Huang,Song Zhang,Zhijun Tu 한국인터넷정보학회 2023 KSII Transactions on Internet and Information Syst Vol.17 No.9
Medical image segmentation techniques based on convolution neural networks indulge in feature extraction triggering redundancy of parameters and unsatisfactory target localization, which outcomes in less accurate segmentation results to assist doctors in diagnosis. In this paper, we propose a multi-level semantic-rich encoding-decoding network, which consists of a Pooling-Conv-Former (PCFormer) module and a Cbam-Dilated-Transformer (CDT) module. In the PCFormer module, it is used to tackle the issue of parameter explosion in the conservative transformer and to compensate for the feature loss in the down-sampling process. In the CDT module, the Cbam attention module is adopted to highlight the feature regions by blending the intersection of attention mechanisms implicitly, and the Dilated convolution-Concat (DCC) module is designed as a parallel concatenation of multiple atrous convolution blocks to display the expanded perceptual field explicitly. In addition, Multi-Head Attention-DwConv-Transformer (MDTransformer) module is utilized to evidently distinguish the target region from the background region. Extensive experiments on medical image segmentation from Glas, SIIM-ACR, ISIC and LGG demonstrated that our proposed network outperforms existing advanced methods in terms of both objective evaluation and subjective visual performance.
( Di Liu ),( Fabiao Hu ),( Wenpeng Wang ),( Dong Wu ),( Xiujuan He ),( Wenyun Zheng ),( Haipeng Liu ),( Xingyuan Ma ) 한국미생물생명공학회(구 한국산업미생물학회) 2017 Journal of microbiology and biotechnology Vol.27 No.8
Escherichia coli heat-labile enterotoxin (LT) and its non-toxic mutant (LTm) are well-known powerful mucosal adjuvants and immunogens. However, the yields of these adjuvants from genetically engineered strains remain at extremely low levels, thereby hindering their extensive application in fundamental and clinical research. Therefore, efficient production of these adjuvant proteins from genetically engineered microbes is a huge challenge in the field of molecular biology. In order to explore the expression bottlenecks of LTm in E. coli, we constructed a series of recombinant plasmids based on various considerations and gene expression strategies. After comparing the protein expression among strains containing different recombinant plasmids, the signal sequence was found to be critical for the expression of LTm and its subunits. When the signal sequence was present, the strong hydrophobicity and instability of this amino acid sequence greatly restricted the generation of subunits. However, when the signal sequence was removed, abundantly expressed subunits formed inactive inclusion bodies that could not be assembled into the hexameric native form, although the inclusion body subunits could be refolded and the biological activity recovered in vitro. Therefore, the dilemma choice of signal sequence formed bottlenecks in the expression of LTm. These results reveal the expression bottlenecks of LTm, provide guidance for the preparation of LTm and its subunits, and certainly help to promote efficient preparation of this mucosal adjuvant protein.
Xudong Di,Danica M.K. Andrews,Charles J. Tucker,Linda Yu,Alicia B. Moore,Xiaolin Zheng,Lysandra Castro,Tonia Hermon,Hang Xiao,Darlene Dixon 생화학분자생물학회 2012 Experimental and molecular medicine Vol.44 No.4
Previously, we found that high doses of genistein show an inhibitory effect on uterine leiomyoma (UtLM) cell proliferation. In this study, using microarray analysis and Ingenuity Pathways AnalysisTM, we identified genes (up- or down-regulated, ≥ 1.5 fold, P ≤ 0.001),functions and signaling pathways that were altered following treatment with an inhibitory concentration of genistein (50 μg/ml) in UtLM cells. Downregulation of TGF-β signaling pathway genes, activin A, activin B,Smad3, TGF-β2 and genes related to cell cycle regulation,with the exception of the upregulation of the CDK inhibitor P15, were identified and validated by real-time RT-PCR studies. Western blot analysis further demonstrated decreased protein expression of activin A and Smad3 in genistein-treated UtLM cells. Moreover, we found that activin A stimulated the growth of UtLM cells, and the inhibitory effect of genistein was partially abrogated in the presence of activin A. Overexpression of activin A and Smad3 were found in tissue samples of leiomyoma compared to matched myometrium, supporting the contribution of activin A and Smad3 in promoting the growth of UtLM cells. Taken together, these results suggest that downregulation of activin A and Smad3, both members of the TGF-β pathway, may offer a mechanistic explanation for the inhibitory effect of a high-dose of genistein on UtLM cells, and might be potential therapeutic targets for treatment of clinical cases of uterine leiomyomas.
Zhaoli Zheng,Fahui Zhu,Di Zhang,Yonghui Xie 대한기계학회 2019 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.33 No.3
A generalized and efficient method is proposed in this paper for parametric response analysis of large-scale asymmetric rotor. If the bearings are anisotropic in asymmetric rotor, the motion of the rotor-bearing system will be governed by ordinary differential equations with periodically time-variant coefficients in both fixed and rotating frame. Response analysis of such parametric system can be attempted by assuming solution based on Hill’s method. If the number of degree of freedoms (DOFs) of the original model is N and the number of terms considered in the truncated assumed solution is m, for response analysis one has to solve a set of equations with (2m+1)N unknowns. Calculating this kind of equations requires a lot of computational costs, and the application of 3D finite element model will lead to greater computational costs. To avoid this costly approach, the fixed-interface component mode synthesis (CMS) is employed to form a reduced-order model (ROM). Although conventional CMS is adequate enough to make response analysis of largescale structures, an optimized scheme still needs to be developed for the response analysis of large-scale asymmetric rotor to achieve high efficiency and accuracy. The optimized scheme concerning a single substructure and multiple substructures are established respectively. Finally, the developed approach is validated through a simple model and a large-scale realistic two-pole generator rotor. And factors that influence the parametric response are investigated.
Yue Zheng,Hengshan Tang,Xiaoyu Wang,Ya Di,Kunling Lu,Jidong Wang 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2017 NANO Vol.12 No.12
The synthesis of aqueous MPA-capped CdTe quantum dots (QDs) via a facile one-pot route was developed. The particle size, optical properties and crystal structure of the as-synthesis QDs were investigated by transmission electron microscopy (TEM), Fourier transform infrared (FTIR), UV-vis absorption and photoluminescence (PL) spectra, respectively. Meanwhile, the effect of reaction conditions, including reaction time, pH and the quantity of sodium citrate (SC) on the growth of CdTe QDs were discussed. Then, the obtained CdTe QDs were successfully used for the detection of trace Cu2+ with high sensitivity and excellent selectivity. The fluorescence intensity of CdTe QDs with Cu2+ concentration showed a linear relationship in the range from 1 x 10 -7 mol/L to 1 x 10 -6 mol/L. The correlation coefficient (R) is 0.9980 and limit of detection (LOD) is 1.0 x 10 -8 mol/L. Moreover, the concentration of Cu2+ in tap water samples was determined based on this sensing system and the recovery test was satisfactory.