http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Tunable Nanostructure of TiO<sub>2</sub>/Reduced Graphene Oxide Composite for High Photocatalysis
He, Di,Li, Yongli,Wang, Jinshu,Yang, Yilong,An, Qier Korean Society of Microscopy 2016 Applied microscopy Vol.46 No.1
In this study $TiO_2$/reduced graphene oxide ($TiO_2/rGO$) bipyramid with tunable nanostructure was fabricated by two-step solvothermal process and subsequent heat-treatment in air. The as-synthesized anatase $TiO_2$ nanocrystals possessed morphological bipyramid with exposed dominantly by (101) facets. Polyethylenimine was utilized during the combination of $TiO_2$ and graphene oxide (GO) to tune the surface charge, hindering the restack of graphene during solvothermal process and resulting in 1 to 5 layers of rGO wrapped on $TiO_2$ surface. After a further calcination, a portion of carbon quantum dots (CQDs) with a diameter about 2 nm were produced owing to the oxidizing and cutting of rGO on $TiO_2$. The as-prepared $TiO_2/rGO$ hybrid showed a highly photocatalytic activity, which is about 3.2 and 7.7 times enhancement for photodegradation of methyl orange with compared to pure $TiO_2$ and P25, respectively. We assume that the improvement of photocatalysis is attributed to the chemical bonding between rGO/CQDs and $TiO_2$ that accelerates photogenerated electron-hole pair separation, as well as enhances light harvest.
The Signaling Mechanism of TGF-β<sub>1</sub> Induced Bovine Mammary Epithelial Cell Apoptosis
Di, He-Shuang,Wang, Li-Gang,Wang, Gen-Lin,Zhou, Lei,Yang, Yuan-Yuan Asian Australasian Association of Animal Productio 2012 Animal Bioscience Vol.25 No.3
The present study showed that Transforming growth factor beta 1 (TGF-${\beta}_1$) can induce apoptosis of bovine mammary epithelial cells. This apoptosis was also observed with phosphorylation of Smad2/3 within 0.5-2 h. Afterwards the signal transferred into the nucleus. Moreover, intracellular free $Ca^{2+}$ concentration was significantly elevated as well as Caspase-3 activated and DNA lysised, thereby inducing the programmed cell death. This signaling pathway of TGF-${\beta}_1$ was blocked by SB-431542 ($10^{-2}{\mu}M$) via inhibiting ALK-5 kinase activity, which thus reversed the anti-proliferation and apoptosis effect of TGF-${\beta}_1$ in mammary epithelial cells. These results indicated that TGF-${\beta}_1$ induced apoptosis of bovine mammary epithelial cells through the ALK-5-Smad2/3 pathway, which plays an important role in inhibiting survival of mammary epithelial cells. Moreover, intracellular $Ca^{2+}$ also played a critical role in TGF-${\beta}_1$-induced cell apoptosis.
Yu Peng,He Xue-jun,Ren Ai-di 보안공학연구지원센터 2014 International Journal of Control and Automation Vol.7 No.12
The alongside replenishment scheduling problem with time constraint determining the partition of the ships, the order of replenishment and the allocation of time to the ships at the same time is analyzed. It is equivalent to a multi-stage flow shop scheduling problem with the object of maximizing the effectiveness value of ship fleet. The problem solving process is divided into three steps, and based on the analysis of the three steps, a heuristic algorithm is proposed. The algorithm firstly considers the time allocated to each ship, and then sequences the ships by heuristic rule combining greed with insertion, finally determines the ships partitioning to the port and standard side. Emulating example with different problems’ scale and time constraints shows that the proposed heuristic algorithm is superior to some other algorithms.
MLSE-Net: Multi-level Semantic Enriched Network for Medical Image Segmentation
Di Gai,Heng Luo,Jing He, Baogang Xie,Pengxiang Su,Zheng Huang,Song Zhang,Zhijun Tu 한국인터넷정보학회 2023 KSII Transactions on Internet and Information Syst Vol.17 No.9
Medical image segmentation techniques based on convolution neural networks indulge in feature extraction triggering redundancy of parameters and unsatisfactory target localization, which outcomes in less accurate segmentation results to assist doctors in diagnosis. In this paper, we propose a multi-level semantic-rich encoding-decoding network, which consists of a Pooling-Conv-Former (PCFormer) module and a Cbam-Dilated-Transformer (CDT) module. In the PCFormer module, it is used to tackle the issue of parameter explosion in the conservative transformer and to compensate for the feature loss in the down-sampling process. In the CDT module, the Cbam attention module is adopted to highlight the feature regions by blending the intersection of attention mechanisms implicitly, and the Dilated convolution-Concat (DCC) module is designed as a parallel concatenation of multiple atrous convolution blocks to display the expanded perceptual field explicitly. In addition, Multi-Head Attention-DwConv-Transformer (MDTransformer) module is utilized to evidently distinguish the target region from the background region. Extensive experiments on medical image segmentation from Glas, SIIM-ACR, ISIC and LGG demonstrated that our proposed network outperforms existing advanced methods in terms of both objective evaluation and subjective visual performance.
( Di Liu ),( Fabiao Hu ),( Wenpeng Wang ),( Dong Wu ),( Xiujuan He ),( Wenyun Zheng ),( Haipeng Liu ),( Xingyuan Ma ) 한국미생물생명공학회(구 한국산업미생물학회) 2017 Journal of microbiology and biotechnology Vol.27 No.8
Escherichia coli heat-labile enterotoxin (LT) and its non-toxic mutant (LTm) are well-known powerful mucosal adjuvants and immunogens. However, the yields of these adjuvants from genetically engineered strains remain at extremely low levels, thereby hindering their extensive application in fundamental and clinical research. Therefore, efficient production of these adjuvant proteins from genetically engineered microbes is a huge challenge in the field of molecular biology. In order to explore the expression bottlenecks of LTm in E. coli, we constructed a series of recombinant plasmids based on various considerations and gene expression strategies. After comparing the protein expression among strains containing different recombinant plasmids, the signal sequence was found to be critical for the expression of LTm and its subunits. When the signal sequence was present, the strong hydrophobicity and instability of this amino acid sequence greatly restricted the generation of subunits. However, when the signal sequence was removed, abundantly expressed subunits formed inactive inclusion bodies that could not be assembled into the hexameric native form, although the inclusion body subunits could be refolded and the biological activity recovered in vitro. Therefore, the dilemma choice of signal sequence formed bottlenecks in the expression of LTm. These results reveal the expression bottlenecks of LTm, provide guidance for the preparation of LTm and its subunits, and certainly help to promote efficient preparation of this mucosal adjuvant protein.
Di Duan,Yong Huang,Ying Zou,Bingju He,Ruihui Tang,Liuxia Yang,Zecao Zhang,Shucai Su,Guoping Wang,Deyi Zhang,Chunhui Zhou,Jing Li,Maocheng Deng 한국식품과학회 2021 Food Science and Biotechnology Vol.30 No.10
Analytical method which combines electronictongue technique and chemometrics analysis is developedto discriminate oil types and predict oil quality. All thestudied Camellia oil samples from pressing, n-hexaneextraction and supercritical CO2 extraction (SCCE), weresuccessfully identified by principal component analysis(PCA) and hierarchical cluster analysis (HCA). Furthermore,multi factor linear regression model (MLRM) wasestablished to predict oil quality, which are indicated byacid value (AV) and peroxide value (POV). The practicalpotential of e-tongue for the discrimination and assessmentof Camellia oils has shown promising application in thecharacterization of Camellia oils in the oil qualityevaluation.
Serra, Heï,di,Choi, Kyuha,Zhao, Xiaohui,Blackwell, Alexander R.,Kim, Juhyun,Henderson, Ian R. Public Library of Science 2018 PLoS genetics Vol.14 No.12
<▼1><P>During meiosis, chromosomes undergo DNA double-strand breaks (DSBs), which can be repaired using a homologous chromosome to produce crossovers. Meiotic recombination frequency is variable along chromosomes and tends to concentrate in narrow hotspots. We mapped crossover hotspots located in the <I>Arabidopsis thaliana RAC1</I> and <I>RPP13</I> disease resistance genes, using varying haplotypic combinations. We observed a negative non-linear relationship between interhomolog divergence and crossover frequency within the hotspots, consistent with polymorphism locally suppressing crossover repair of DSBs. The <I>fancm</I>, <I>recq4a recq4b</I>, <I>figl1</I> and <I>msh2</I> mutants, or lines with increased <I>HEI10</I> dosage, are known to show increased crossovers throughout the genome. Surprisingly, <I>RAC1</I> crossovers were either unchanged or decreased in these genetic backgrounds, showing that chromosome location and local chromatin environment are important for regulation of crossover activity. We employed deep sequencing of crossovers to examine recombination topology within <I>RAC1</I>, in wild type, <I>fancm</I>, <I>recq4a recq4b</I> and <I>fancm recq4a recq4b</I> backgrounds. The <I>RAC1</I> recombination landscape was broadly conserved in the anti-crossover mutants and showed a negative relationship with interhomolog divergence. However, crossovers at the <I>RAC1</I> 5′-end were relatively suppressed in <I>recq4a recq4b</I> backgrounds, further indicating that local context may influence recombination outcomes. Our results demonstrate the importance of interhomolog divergence in shaping recombination within plant disease resistance genes and crossover hotspots.</P></▼1><▼2><P><B>Author summary</B></P><P>Sexually reproducing plants and animals produce gametes with half the number of chromosomes, which can participate in fertilization. A specialized cell division called meiosis generates gametes, where the chromosomes are copied once and segregated twice. A further key feature of meiosis is that chromosomes physically pair and undergo reciprocal exchanges, called crossovers. Due to independent chromosome segregation and crossovers, meiosis creates gametes that are genetically diverse, which has a major effect on patterns of sequence variation in populations. Interestingly, the frequency of crossover is also highly variable along the lengths of chromosomes and tends to be concentrated in narrow hotspots. Here we studied two crossover hotspots in detail that are located within disease resistance genes, using the model plant Arabidopsis. We show that within these hotspots, greater levels of genetic difference between the recombining chromosomes locally inhibits crossover formation. We also show that hotspots within one of these resistance genes are surprisingly resistant to genetic backgrounds that increase crossovers elsewhere in the genome. This indicates that patterns of polymorphism and hotspot location along the chromosome are both important for control of recombination activity.</P></▼2>
Chao He,Shunhua Zhou,Honggui Di,Xinwen Yang 대한토목학회 2019 KSCE Journal of Civil Engineering Vol.23 No.11
This paper analytically investigates the dynamic interaction of two neighboring tunnels in the saturated soil. The twin tunnels are simulated using the Flügge shell theory, and the surrounding soil is modelled by the Biot's theory. The solution for cylindrical waves in saturated porous media is derived by applying the potential decomposition. The addition theorem is subsequently introduced to translate cylindrical waves, thereby satisfying the boundary conditions at the interfaces between the twin tunnels and saturated soil. Numerical examples are presented to analyze soil vibrations under different loading conditions calculated by different tunnel models. The neighboring tunnels have strong dynamic interaction, resulting in the insertion gain up to ± 20 dB. It is recommended to account for the interaction between neighboring tunnels when calculating the vibrations from underground railways.