흡연이 Gastric Emptying Time에 미치는 영향

조민구(Min Koo Cho),정순영(Sun Young chung),김소연(So Yon Kim),문희승(Mun Hee Seung),김진석(Jin Suk Kim),이석호(Suk Ho Lee),이권전(Gwon Jun Lee) 대한소화기학회 1990 대한소화기학회지 Vol.22 No.2

The effect of cigarette smoking on gastric emptying was examined in 25 healthy volunteers by means of Tc-sulfur colloid labeled-solid meal. The volunteers underwent GET measurement two times: before smoking and after smoking two cigarettes. Before smoking, the each average of GET T25% T50%, T75%, was 18 +- 5.2, 40 +- 6.7, 90 +- 18.4 min and after cigarette smoking the each average of GET T25%, T50%, 75% was 30 +- 8.3, 64 +- 12.6, 114 +- 7.0 min. We concluded that cigarette smoking significantly delayed gastric emptying of a solid meal (p< 0.05).

전해수 생성온도에 따른 그람양성균과 그람음성균의 살균 효과

이정민(Jeong Min Lee),정현정(Hyun-Jung Chung),방우석(Woo Suk Bang) 한국식품영양과학회 2016 한국식품영양과학회지 Vol.45 No.8

본 연구에서는 다양한 온도의 물로 제조한 전해수를 이용하여 그람양성균과 그람음성균에 대한 살균력을 확인하였다. 전해수의 물성은 물의 온도가 높아질수록 pH와 유효 잔류염소 농도 값이 높아졌으나, 산화환원전위 값은 감소하였다. 4, 22, 40°C의 물을 이용하여 생성한 전해수에 Escherichia coli O157:H7을 15분 동안 처리하였을 때 각각 1.18, 4.47, 5.46 log CFU/mL 감소하였으며, Staphylococcus aureus는 각각 0.72, 4.90, 5.54 log CFU/mL 감소하였다. 이를 통해 전해수를 생성하는 물의 온도가 증가할수록 살균 효과가 높아지는 것을 알 수 있다. 4, 40°C의 물을 이용하여 생성한 전해수에 균주를 처리한 경우, Listeria monocytogenes의 D 값(일정한 온도에서 90 %의 미생물이 사멸하는 데 걸리는 시간)이 각각 6.60, 1.57분으로 나타나 가장 낮았으며, 22°C 전해수에서는 Salmonella Enteritidis의 D 값이 2.92분으로 가장 낮은 값을 나타내었다. 그람양성균과 그람음성균을 비교하였을 때 모든 온도에서 D 값에 대한 유의적인 차이는 나타나지 않았다(P>0.05). 본 연구 결과는 전해수를 제조할 경우 높은 온도의 물을 이용하여 제조하는 것이 미생물 살균 효과가 높다는 것을 보여주고 있다. 따라서 전해수를 생성할 때 물의 온도를 고려하여 높은 살균력을 나타내는 전해수를 생성할 수 있으며, 이는 물의 온도 외에도 물의 경도나 물의 종류 등을 고려하여 최적의 살균 전해수를 제조함으로써 식품산업에 적용하기 위한 자료로 활용될 것으로 기대된다. Electrolyzed activated water (EAW) has been reported to exhibit strong bactericidal effects on foodborne microorganisms. However, the disinfection efficacy of EAW is affected by factors such as water source and hardness. This study investigated bactericidal effects of EAW against three gram-positive (Bacillus cereus, Listeria monocytogenes, and Staphylococcus aureus) and three gram-negative (Cronobacter sakazakii, Escherichia coli O157:H7, and Salmonella Enteritidis) foodborne pathogens. Six strains were treated with EAW prepared at different water temperatures (4, 22, and 40°C) for 15 min, and D-values were generated. The results show that the lowest D-values for Lis. monocytogenes by EAW produced at 4°C and 40°C were 6.60 and 1.57 min, respectively. The lowest D-value for Sal. Enteritidis by EAW produced at 22°C was 2.92 min. D-values of all strains treated by EAW produced at 40°C decreased significantly compared to those treated by EAW produced at 4°C (P<0.05). These results demonstrate that applying EAW produced at warm temperature is more effective for reducing foodborne pathogens for food safety.

오이 추출물에 존재하는 Superoxide Dismutase의 열안정성

김은애(Eun-Ae Kim),김기남(Gi-Nahm Kim),길지은(Ji-Eun Kil),이민경(Min-Kyung Lee),김석환(Suk-Hwan Kim),서정식(Chung-Sik Suh),박인식(Inshik Park) 한국식품영양과학회 1999 한국식품영양과학회지 Vol.27 No.6

오이속의 조효소액에 존재하는 Superoxide dismutase(SOD)활성의 pH 안정성은 pH 8.0에서 가장 안정하였고 pH5.0~9.0 사이의 범위에서는 비교적 안정하였다. 최적 온도는 25℃였고 열 안정성은 60℃까지는 안정하였다. 100℃에서 5분간 보관하였을 경우에는 12%만이 남아있었다. 오이에 존재하는 SOD 활성이 섭취 후에도 안정한가를 확인하기 위한 실험에서는 위속의 pH와 동일하도록 오이속의 조효소액의 pH를 2.0으로 변형시킨 후 36.7℃에서 3시간 동안 보관 후에 잔존활성이 10%였고, 장내의 환경인 pH 7.0으로 바꾸어 6시간 동안 둔 후 잔존하는 SOD의 활성은 25%로 활성이 증가되었다. 다양한 열처리 후에 잔존하는 오이의 SOD활성은 오이속은 데치기에서(끓는 물에서 2분) 25%, 껍질은 찌는 동안에(3분) 53%, 그리고 속과 껍질로 분리하지 않은 오이는 데치기에서 27%의 활성잔존률을 보였다. 4℃에서는 20일간 보관한 후에 오이속의 조효소액은 81%활성이 있었고, 30℃에서는 17%의 활성이 남아 있었다. 투석한 결과 SOD의 활성은 변화가 없었으므로 오이속에 존재하는 SOD는 적어도 분자량이 12,000 이상의 물질로 추정된다. The superoxide dismutase(SOD) in peeled pericarp of cucumber was most stable at pH 8.0 and relatively stabe between pH 5.0 and 9.0. The enzyme was stable up to 60℃ and retained 12% by heat treatment at 100℃ for 5 min. At pH 2.0, the peeled pericarp enzyme activity was decreased to 10% by incubation for 3 hrs. However, the enzyme activity was increased above 25% after incubating the enzyme at pH 7.0 for 6 hrs. Retention of SOD activity in cucumber by various heating methods was also measured. The residual SOD activities of peeled pericarp and whole cucumber was estimated to be 25% and 27% after blanching (2 min), respectively. The skin enzyme retained 53% of its activity after steaming (3 min). When the peeled peri carp enzyme was incubated at 4℃ for 20 days, the enzyme activity remained about 81%. However, when the enzyme incubated at 30℃ for 20 days, the peeled pericarp enzyme activity decreased to 17% of its original activity. The enzyme activity of peeled pericarp cucumber was not changed after exhaustive dialysis for 3 days, which indicated that the SOD activity in cucumber seems to have molecular weight above 12,000.

Sustained-release praziquantel tablet : pharmacokinetics and the treatment of clonorchiasis in beagle dogs

Hong, Sung-Tae,Lee, Sang Hyup,Lee, Seung-Jin,Kho, Weon-Gyu,Lee, Mejeong,Li, Shunyu,Chung, Byung-Suk,Seo, Min,Choi, Min-Ho 梨花女子大學校 藥學硏究所 2003 藥學硏究論文集 Vol.- No.12

Praziquantel is rapidly absorbed and secreted; and thus fractional doses are recommended for the treatment of cestode and trematode infections. In the present study, we developed a new praziquantel tablet formula allowing sustained-release (SRP). In vitro dissolution of SRP tablets showed that praziquantel at 300 ㎎/tablet combined with hydroxypropyl methylcellulose dissolved completely at a constant rate over 10 h, whereas the conventional praziquantel tablet (PZQ) was only 40% dissolved. Pharmacokinetic studies in dogs confirmed that SRP was absorbed more slowly than PZQ. The mean value of the area under the concentration/time curve from 0 h to the final observation time, the maximum concentration in serum, and the time of maximum concentration in serum for SRP were 3.471.500 ng/min for 0.25 ㎖, 10,300 ng for 0.25 ㎖, and 192 min. while the values for PZQ were 688,600 ng/min for 0.25㎖, 2,500 ng for 0.25㎖, and 135 min. The cure rate in dogs with a heavy infection (500 metacercariae) treated with a single dose of SRP (150 ㎎/tablet) at 50 ㎎/㎏ was 80%, while in dogs treated with a single dose of SRP (300 ㎎/tablet) at 30 mg/kg it was 60%, and the cure rate with PZQ was 20%. In each case. the egg reduction rate was similar (over 90%). No abnormal liver functions or hepatic or renal pathologies were observed in dogs administered with SRP at 30 ㎎/㎏. The SRP tablet showed sustained release and slow absorption; and it had an improved anthelmintic efficacy against Clonorchis sinensis in experimental dogs. compared with conventional praziquantel.

Citrus fruit extracts with carvacrol and thymol eliminated 7-log acid-adapted <i>Escherichia coli</i> O157:H7, <i>Salmonella</i> typhimurium, and <i>Listeria monocytogenes</i>: A potential of effective natural antibacterial agents

Chung, Doohyun,Cho, Tae Jin,Rhee, Min Suk Elsevier 2018 Food Research International Vol.107 No.-

<P>Despite the widespread belief that citrus fruit extracts (CFEs) are microbiologically safe due to their acidity, limited bactericidal effect results in low applicability as antibacterial agent and outbreaks occurred by acid-adapted pathogens. Here, we examined the antibacterial effects of CFEs [lime (Citrus medica), lemon (Citrus limon), calamansi (Citrus microcarpa)] combined with essential oil components (EOCs; carvacrol and thymol) against non-acid-adapted/acid-adapted Escherichia coli 0157:H7, Salmonella Typhimurium, and Listeria monocytogenes under 22 degrees C for 5 min. CFEs (< 20%) alone or small amounts of EOCs (2.0 mM; 0.032%) alone could not inactivate the target bacteria effectively. However, combined treatments exhibited marked synergy: CFE + EOCs eliminated all the bacteria (> 6.9 log CFU/ml). Among the CFEs tested, the highest synergism was shown by calamansi, an exotic citrus fruit previously unrecognized as an antibacterial agent. Although acid-adaptation improved bacterial survival, calamansi (< 20%) + EOCs (< 0.032%) completely inactivated even the most resistant pathogen (E. coli 0157:H7). Validation test also showed that all tested commercial juice products also eliminated acid -adapted pathogens when used with EOCs. Physicochemical analysis of tested CFEs (pH measurement and HPLC analysis of components) revealed that low pH and flavanone (hesperidin) did not contribute to the synergistic bactericidal effects. Rather, the high citric acid content is likely to contribute to the strong synergistic effect with EOCs by damaging susceptible bacterial membranes. Sensory scores for CFEs were not altered by addition of EOCs at concentrations up to 1.5 mM. This study provides new insight into the utility of CFEs with EOCs to improve not only the microbiological safety of food products containing CFEs but also their applicability as natural antibacterial complex.</P>

치아미백제가 우치의 색상에 미치는 영향

정석민,박경희,박영준 대한치과기재학회 2001 대한치과재료학회지 Vol.28 No.1

The purpose of this study was to evaluate the effects of commercial home-tooth bleaching agents in the color of tooth. Thirty sound extracted bovine teeth were randomly divided into six groups. The color differences between before and after treatment with four types of tooth bleaching agents (Nite White ExcelR, Insta-BriteTM, OpalescenceR, and Plus WhiteR), one type of whitening toothpaste (Natural WhiteR) and untreated control group were evaluated. The results were as follows; 1.By 2 week home tooth bleaching agent applications, the values (L*) of bovine teeth increased as high as 2.18 ∼ 6.73. 2.Insta-Brite induced significantly greater ΔL* and ΔE* than other tested groups by 2 week bleaching agent treatments(p<0.01). 3.Plus White which was applied for 5 minutes twice a day for 2 weeks induced significantly lesser ΔL* (2.18) and ΔE* (2.80) than those in Insta-Brite (ΔL*=5.30, ΔE*=6.73), Nite White Excel (ΔL*=4.24, ΔE*=4.90), Opalescence (ΔL*=3.78, ΔE*=4.92) which were applied for 8 hours a day for 2 weeks (p<0.01). These results demonstrate that all the commercial home-tooth bleaching agents have appreciable bleaching effect on bovine teeth, and the effects of home-tooth bleaching agents which are used during night time are affected by viscosity as well as content of carbamide peroxide. The whitening effect of home tooth bleaching agents that are used through nighttime is greater than that of short time-applying tooth bleaching agent.

치아미백제가 치아의 색상에 미치는 영향

정석민 대구보건대학 2000 대구보건대학 論文集 Vol.20 No.-

This study was purposed to evaluate the effect of commercial home tooth bleaching agent application on the color of tooth. Fifty sound extracted teeth were randomly divided and the color differences between before and after treatment with 5 types of tooth bleaching agents (Nite White Excel, Insta-Brite, Opalescence, Plus White and StarBrite Contrast _(P.M).) were evaluated. Teeth were treated with 5 types of bleaching agents and the color changes were evaluated. The results were as follows; 1. By 4 weeks bleaching agents treatment, the difference of value (L^(*)) of the tooth were increased as high as 3.61 ~ 7.75 and the color difference (E^(*)) showed as high as 9 ~14. 2. L^(*) and E^(*) between 2 week treated- and 4 week treated-groups did not show significant changes (p>0.05), except L^(*) and E^(*) in the cases of Plus White and E in the cases of StarBrite Contrast treated group (p<0.05). 3. Nite White Excel among tested bleaching agents induced greatest L^(*) increase for teeth showing significant changes of L^(*) by 2 weeks bleaching agent treatments and by 4 weeks bleaching agent treatments except in the case of Opalescence (p<0.05). Nite White Excel induced significantly greater E^(*) for teeth by 2 and 4 weeks bleaching agent treatments when comparing to the E^(*) induced by all other tested bleaching agents groups (p<0.05). These results demonstrate that all the commercial tooth bleaching agents have appreciable bleaching effect on teeth.

치아미백제가 치아 및 치과용 심미수복재의 경도에 미치는 영향

정석민 대한치과기재학회 1999 대한치과재료학회지 Vol.26 No.4

The purpose of this study was to evaluate the effect of tooth bleaching agents application on the hardness of tooth and dental restorative materials. In order to evaluate the effect of intraoral aging of the materials(3 composite resins; Palfique Estelite Prodigy, TPH Spectrum, and 1 compomer; Dyract) on the degree of hardness change induced by tooth bleaching agents, each material group was subdivided(thermocycling and non-thermocycling). Ten teeth and three specimens per material group were treated with 5 types of bleaching agents(Nite White Excel, Insta-Brite, Opalescence, Plus White, and StarBrite ContrastP.M. and evaluated. The results were as follows: 1. Nite White Excel and StarBrite Contrast bleaching agent treatments applied to tooth significantly reduced Vickers hardness value(p<0.05). 2. Dyract demonstrated significant decrease in Vickers hardness value by all kinds of the tooth bleaching agents in both of the thermocycling and non-thermocycling subgroup(p<0.05). 3. By Opalescence and Plus White tooth bleaching agents, all esthetic restorative materials demonstrated significant decrease in Vickers hardness value in both of the thermocycling and non-thermocycling subgroup(p<0.05). 4. Prodigy showed higher Vickers hardness value by thermocycling compared to that of non-thermocycling group treated with all kinds of tooth bleaching agents. 5. Palfique Estelite treated with Plus White and StarBrite Contrast tooth bleaching agents showed significant difference in the amount of Vickers hardness value changes by themocycling compared to those of non-thermocycling group(p<0.05).

치아에 대한 치아미백제의 효과에 관한 연구

정석민 광주보건대학 2002 論文集 Vol.27 No.-

This study was purposed to evaluate the effect of commercial home tooth bleaching agent application on the color of tooth. Fifty sound extracted teeth were randomly divided into 5 groups and were evaluated by the color differences between before and after treatment with 5 types of tooth bleaching agents (Nite White Excel, Insta-Brite, Opalescence, Plus White and StarBrite Contrast _(P.M.)). Teeth were treated with 5 types of bleaching agents and the color changes were evaluated. The results were as follows; 1. By 4 weeks bleaching agents treatment, the difference of value (L^(*)) of the tooth were increased as high as 3.61 ∼ 7.75 and the color difference (E^(*)) showed as high as 9 ∼ 14. 2. L^(*) and E^(*) between 2 week treated- and 4 week treated-groups did not show significant changes (p>0.05), except L^(*) and E^(*) in the cases of Plus White and E^(*) in the cases of StarBrite Contrast treated group(p<0.05). 3. Nite White Excel among tested bleaching agents induced greatest L^(*) increase for teeth showing significant changes of L^(*) by 2 weeks bleaching agent treatments and by 4 weeks bleaching agent treatments except in the case of Opalescence (p<0.05). Nite White Excel induced significantly greater E^(*) for teeth by 2 and 4 weeks bleaching agent treatments when comparing to the E^(*) induced by all other tested bleaching agents groups (p<0.05), These results demonstrate that all the commercial tooth bleaching agents have appreciable bleaching effect on teeth.

케이슨 튜브 가물막이의 시공안정성 검토

정성교,김성렬,N. T. Dung,김민석 東亞大學校 建設技術硏究所 2005 硏究論文集 Vol.29 No.1