돼지 卵子의 透明帶에 대한 單一클론抗體生産과 그 特性에 關한 硏究

金鐘培,劉永春,金昌圭,權五中,鄭盛元,鄭吉生 건국대학교 동물자원연구센터 1991 動物資源硏究誌 Vol.16 No.-

本 試驗은 單一클론抗體의 강한 特異性과 抗體性質의 不變性을 이용하여 發生學的 側面에서 哺乳動物 卵子의 透明帶의 機能과 構造를 이해하고, 또한 種特異的인 精子 受容體의 存在 및 生化學的 構造를 규명하기 위한 기초연구로서, 돼지 卵子의 透明帶를 免疫原으로 하여 BABL/c 생쥐로부터 單一클론抗體를 생산하고 그 특성을 구명하였던 바 다음과 같은 결과를 얻었다. 1) 3마리의 BABL/c 생쥐(YⅠ, YⅡ, ZI)에 돼지卵子의 透明帶를 免疫化하고, 複合抗體 生成을 확인한 후 생쥐의 脾臟細胞와 Myeloma(SP2/O-Ag14)를 polyethylene glycol를 融合을 실시한 결과 각각 25.8%, 54.5% 그리고 59.7%의 融合效率을 나타내었으며, ELISQ에 의해 陽性反應을 조사한 결과 각각 17.3%, 32.6% 그리고 6.2% 陽性反應 效率을 나타내었다. 2) YI에서 강한 陽性反應을 보인 6개의 well에 대한 cloning을 실시하고 抗體檢證을 행한 결과 20.8% ∼ 48.4%의 Cloning效率과 54.6% ∼ 82%의 陽性反應 效率을 나타내었다. 3) 강한 陽性反應을 나타낸 항체에 대해 sandwich ELISA法에 의해 isotype을 決定하였던바 2E93C(YⅠ), 3E83E7(YⅠ), 4E3(YⅡ)각각 IgG₂b, IgG₂a, IgM으로 확인되었다. 4) Isotype이 決定된 2E93C9(YⅠ), 3E84E7(YⅠ), 4E3(YⅡ)의 세포를 생쥐의 腹腔에 주사하여 얻은 腹水를 indirect ELISA法에 의해 titer를 決定한 결과 모두 1:400,000 이상의 높은 titer를 나타내었다. 5) 處理區로서 單一클론抗體의 腹水와 對照區로서 normal mouse serum이 각각 2%씩 함유된 배양액속에서 난자를 배양한 후 顯徵鏡下에서 관찰했을 때 對照區에서 배양된 난자의 표면은 정상적인 형태를 나타냈으나 處理區에서 배양된 卵子는 표면에 뚜렷한 沈澱層을 형성하였다. 6) 處理區와 對照區 卵子를 Rabbit anti-mouse IgG-FITC가 1% 함유된 배양액속에서 배양하고 洗滌한 후 螢光顯徵鏡下에서 관찰한 바 處理區의 卵子는 透明帶 주위에서 螢光이 나타났으나, 對照區에서는 나타나지 않았다. This study was carried out ot produce and characterize monoclonal antibodies against porcine zona pellucida, and undertaken as a basic study to develop immunocontraceptive vaccine and to investigate the function of zana pellucida in early fertilization process. The results obtained were summarized as follows: 1. Spleen cells of three BALB/C mice(YⅠ, YⅡ and ZI) which were immunized with porcine zona pelucida were fused with myeloma cells(SP2/O-Ag14) by polyethylene glycol. At the result of fusion, fusion efficiency was 25.8 , 54.5% and 59.7% and positive efficiency 17.3%, 32.6% and 6.2%, respectively. 2. Cloning efficiency was shown to be from 20.8% to 48.4% and positive efficiency of them were 54.6% to 82%. 3. Sub-isotypes of three strong positive antibodies, 2E93C(YⅠ), 3E83E7(YⅠ) and 4E3(YⅡ) were determined by sandwich ELISA method and shown to be as IgG2b, IgH2a and IgM, respectively. 4. The titers of three ascitic fluids containing antibodies, 2E93C9(YⅠ), 3E84E7(YⅠ) and 4E3(YⅡ) were determined by indirect ELISA and all of them showed above 1:400,000. 5. The layer of precipitate was observed on the zona incubated with medium containing 2% ascitic fluid of monoclonal antibody while the eggs treated with 2% normal mouse serum did not. 6. Porcine eggs incubated with medium containing 2% ascitic fluid of monoclonal antibody and followed by subsequent incubation with Rabbit anti-mouse IgG-FITC conjugate showed strong fluorescent light on the zona surface while the zona of normal mouse serum-treated eggs did not show fluorescence.

한국잔디 수집계통들 중에서 우수계통들의 생육특성 비교

임용우(Y. W. Rim),김기용(K. Y. Kim),김맹중(M. J. Kim),성병렬(B. R. Sung),임영철(Y. C. Lim),정의수(E. S. Chung),신홍균(H. K. Shin),김용선(Y. S. Kim) 한국잡초학회·한국잔디학회 2003 Weed & Turfgrass Science Vol.17 No.2,3

2001년 수집된 한국잔디 133계통들 중에서 우수한 계통들을 선발하기위하여 밀도(품질),<br/> 피복성, 녹색기간, 내병성, 출수유무(종자수) 등의 주요특성과 그 외 생육특성들이 조사되었다. 생육특성이 우수한 6계통이 선발되었으며 그 결과를 요약하면 다음과 같다.<br/> 수집된 133계통들 중에서 가장 녹색기간이긴 계통은 J01067으로 11월초까지 녹색도를 유지하였다. J01106 및 J01129 계통들은 엽폭이 각각 1.5mm 및 2mm 정도로 매우 좁으며, 밀도가 높고, 피복성도 좋은 것으로 나타났으며, 내병성도 강하였다. J01122 계통은 들잔디 계통들 중에서 유일하게 내병성이 강한 계통으로 선발되었다. J01128 계통은 엽폭이 3.2mm로 중엽에 속하였으며, 대비품종인 Sunburst 와 비슷하였다. 이 계통의 경우도J01106, J01129 계통들과 마찬가지로 밀도가 높고 내병성이 강한 것으로 나타났다.<br/> 수집된 133계통들의 형태적 분류를 위하여 5가지의 생육특성을 사용하여 크게 세가지 군집<br/> 으로 분류하였으며, 그 중에서 대비품종으로 사용된 6품종들과 선발된 우수 6계통의 분류는<br/> 다음과 같다. 제 1군집에 속하는 품종 및 계통은 Belare, Meyer, 안양중지(Anyang-jungji), J01067, J01112 등이 포함되었으며, 제 2군집으로는 S-94, J01105가 포함되었고, 제 3군집에는 Sunburst, 건희(Konhee), J01106, J01128, J01129 등이 포함되었다. Growth characteristics such as density(quality), covering speed, green period, disease resistance, heading existence(number of seeds) and other characteristics were examined for selection of superior lines among the 133 zoysiagrass lines collected in 2001. Six superior lines were selected and the results were summarized as follows.<br/> Superior line, J01067 was longest for green period among the 133 zoysiagrasses and stayed green until the beginning of November. Leaf width of J01106 and J01129 lines was very narrow as 1.5㎜ and 2㎜, respectively and superior for density(quality), covering speed and disease resistance. J01122 line was selected for strongest disease resistance among the lines of Zoysia japonica. Leaf width of J01128 was 3.2㎜ showing midium type and similar to standard cultivar, Sunburst. This line also showed higher density and strong disease resistance like J01106 and J01129.<br/> Five growth characteristics for morphological classification of 133 zoysiagrass lines<br/> was used and divided into 3 cluster groups. Of 133 lines, 6 standard cultivars and 6 superior lines were classified as follows. First cluster group contained Belare, Meyer, Anyang-jungji, J01067, J01122, and second group contained S-94 and J01105, and third group contained Sunburst, Konhee, J01106, J01128 and J01129.<br/> <br/>

The antimicrobial peptide human cationic antimicrobial protein-18/cathelicidin LL-37 as a putative growth factor for malignant melanoma

Kim, J.E.;Kim, H.J.;choi, J.M.;Lee, K.H.;Kim, T.Y.;Cho, B.K.;Jung, j.Y.;Chung, K.Y.;Cho, D.;Park, H.J. Sookmyung Women's University Research Institute of 2011 여성과 건강 Vol.6 No.2

Background Recent evidence suggests cathelicidin LL-37 to be a growth factor for various human cancers such as lung cancer, ovarian cancer and breast cancer. However, the effect of LL-37 against malignant skin cancer has not been reported. Objectives To investigate whether the human cathelicidin LL-37 is involved in the carcinogenesis of various skin tumours. Methods Human cationic antimicrobial protein-18 (hCAP-18)/LL-37 production in several cell lines including HaCaT, a chronic myelogenous leukaemia (CML) cell line and various melanoma cell lines was examined using reverse transcription polymerase chain reaction and enzyme-linked immunosorbent assay. Immunohistochemical analysis of melanoma, nonmelanoma skin cancer and precancerous and benign skin lesions was performed. After adding LL-37 to a melanoma cell line, tumour cell proliferation, migration and invasion were investigated. Results Human malignant melanoma cell lines overexpressed hCAP-18/LL-37 mRNA and peptide compared with HaCaT and CML cell lines. Immuno- histochemistry showed that the peptide was strongly expressed in malignant melanoma and moderately expressed in squamous cell carcinoma, whereas basal cell carcinoma, precancerous lesions and seborrhoeic keratosis showed no or weak expression. LL-37 also stimulated melanoma cell proliferation, migration and invasion in vitro. Conclusions Cathelicidin LL-37 was primarily expressed in human malignant skin cancer. LL-37 promoted melanoma cell proliferation, migration and invasion in vitro. We report that an increase in the level of LL-37 is associated with malignant skin tumours such as malignant melanoma. These results highlight the importance of LL-37 in the malignant tendency of skin tumours.

[디젤엔진부문] 이종연료 층상분사를 이용한 디젤엔진의 NOx와 soot 동시 저감에 관한 연구

강병무(B.M. Kang),김종률(J.Y. Kim),이태원(T.W. Lee),정성식(S.S Chung),하종률(J.Y. Ha) 한국자동차공학회 2000 한국자동차공학회 춘 추계 학술대회 논문집 Vol.- No.-

<br/> ~~.A\-%7}t'--c:j-~ 2000\15:. ~;>jI~t:1J~<br/> 2000-03-2041<br/> 01<br/> 3S. 91 ~ ~A~~<br/> A<br/> L.s:.<br/> OI<br/> Ho~<br/> c:"~.n. C::>C>c r2. C:::>L ;<br/> C/~2!!~~ NOx$ soot ~AI AI~OU ~~ eF?<br/> 7J'!!-';'-, 7,J'6-it(%ottJI-o:}jj! tJI~~), 01I11~(~~ ~~tJI-O:}), '\J'\J-6j, "6J-'6-iH%ottJI-o:}jj!)<br/> A Study on the Simultaneous Reduction of :>iOx and Soot<br/> with Dual Fuel Stratified Injection System in a Diesel Engine<br/> B. 1\1. Kang, ]. Y. Kim(Graduated School of Dong- A L'niv'>, T. W. Lee(Changwon CQUage), S. S. Chung, ]. Y. Ha(Dong-A Univ,)<br/> ABSTRACT<br/> To investigate the method of the simultaneous reduction of Soot and NOx, we developed a new conception for dual fuel stratified injection system in a Diesel Engine.<br/> Some parts of the injection system, ie. nozzle holder, delivery vale. were remodeled to inject diesel and methanol in order from one injector. The Quantity of diesel and methanol was controled precisely by micrometers that were mounted at control lack of injection pump.<br/> The real injection Quantity ratio of dual fuel was certificated by volumetric ratio at injection Quantity experiment. We could confirm the capability that soot and NOx simultaneously were reduced by diesel-methanol stratified injection from the results of combustion and exhaust characteristic.<br/>

The pharmacological stimulation of Nurr1 improves cognitive functions via enhancement of adult hippocampal neurogenesis

Kim, J.i.,Jeon, S.G.,Kim, K.A.,Kim, Y.J.,Song, E.J.,Choi, J.,Ahn, K.J.,Kim, C.J.,Chung, H.Y.,Moon, M.,Chung, H. Elsevier 2016 Stem cell research Vol.17 No.3

<P>The nuclear receptor related-1 (Nurr1) protein plays an important role in both the development of neural precursor cells (NPCs) and cognitive functions. Despite its relevance, the effects of Nurr1 on adult hippocampal neurogenesis have not been thoroughly investigated. Here we used RT-PCR, western blot, and immunocytochemistry to show that adult hippocampal NPCs abundantly express Nurr1. We then examined the effect of Nurr1 activation on adult hippocampal NPCs using amodiaquine (AQ), an anti-malarial drug that was recently discovered to be a Nurr1 agonist. Cell proliferation assay showed that AQ significantly increased cell proliferation. AQ-treated NPCs showed increased levels of phosphorylation of Akt and ERK1/2 whereas AQ-treated Nurr1 siRNA-transfected NPCs showed no changes in those levels. Further immunocytochemical and immunohistochemical analyses confirmed the stimulating effect of Nurr1 agonist on the proliferation and differentiation of adult hippocampal NPCs both in vivo and in vitro. In addition to its effects on proliferation and differentiation of NPCs, AQ-treated mice showed a significant enhancement of both short-and long-term memory in the Y-maze and the novel object recognition test. These data suggest that activation of Nurr1 may enhance cognitive functions by increasing adult hippocampal neurogenesis and also indicate that Nurr1 may be used as a therapeutic target for the treatment of memory disorders and cognitive impairment observed in neurodegenerative diseases. (C) 2016 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license.</P>

Pretreatment HBeAg level and an early decrease in HBeAg level predict virologic response to entecavir treatment for HBeAg‐positive chronic hepatitis B

Kwon, J. H.,Jang, J. W.,Lee, S.,Lee, J.,Chung, K. W.,Lee, Y. S.,Choi, J. Y. Blackwell Publishing Ltd 2012 Journal of viral hepatitis Vol.19 No.2

<P><B>Summary. </B> There are few reports on hepatitis B e antigen (HBeAg) titres during nucleos(t)ide analogues treatment. We investigated the changes in HBeAg levels in patients treated with entecavir and the usefulness of HBeAg quantification for predicting antiviral response. Ninety‐five consecutive HBeAg‐positive patients treated with entecavir for more than 48 weeks were enrolled. Serum levels of hepatitis B surface antigen (HBsAg), HBeAg and HBV DNA were assessed at 4‐week intervals to week 24 and thereafter at 12‐week intervals. Virologic response (Y1VR) was defined as an undetectable HBV DNA level at week 48 of therapy. During 48 weeks, HBeAg and HBV DNA level decreased significantly in a biphasic manner and HBsAg level tended to decease. Fifty‐three patients (55.8%) attained Y1VR. Pretreatment HBeAg levels were significantly lower in the Y1VR group than in no Y1VR group. At week 4 and 12 of therapy, 25% and 41.4% of patients showed a decrease of HBeAg levels with >0.5 log<SUB>10</SUB> and >1.0 log<SUB>10</SUB> from baseline, respectively. These patients achieved more Y1VR than those with less decrease of HBeAg levels (97.7%<I>vs</I> 22.2% and 86.2%<I>vs</I> 29.3%, respectively). HBeAg level at week 12 had higher predictive values for Y1VR than HBV DNA level. Multivariate analysis revealed that a pretreatment HBeAg level of <360 PEIU/mL and the reduction in HBeAg level >1.0 log<SUB>10</SUB> at week 12 were associated with Y1VR. These results suggest that pretreatment HBeAg level and an early decrease in HBeAg level are useful measurements for predicting one‐year virologic response during entecavir treatment.</P>

200 GeV/핵자 유황이온과 핵건판핵의 충돌에 의해 생성된 헬륨 파쇄핵의 극한파쇄 연구

김동철,송진섭,윤천실,정성헌,박인곤,김종오,김철수,김태연,이승희,조재희,천병구,김재률,김준원,김태익,박명렬,장한일,임인택 慶尙大學校 기초과학연구소 1992 基礎科學硏究所報 Vol.8 No.-

고에너지 중이온 원자핵과 핵건판의 충돌에서, 200GeV/핵자 유황이온에 의해 생성된 파쇄 헬륨핵(Z=2)의 실험실계의 방출각 분포는 표적핵에 무관한 회귀공식. dN=exp[a+k exp(η-y_b)]d[exp(η-y_b)]로 잘 표현된다. 여기에서 의사신속도 η=-ln[tan(θ/2)]이고, y_b는 실험실계의 입사입자(^32S)의 신속도이다. 이 공식에 의한 적합에서 k=-0.057±0.008로 얻어진다. 즉, 핵건판과 고에너지 중이온의 충돌에서 파쇄 헬륨핵의 exp(η-y_b)의 분포는 "극한파쇄" 현상을 잘 설명하고 있다. The angular distribution of emission angle θ of helium (Z=2) produced in the collisions of incident particles of 200 GeV/nucleon ^32S in nuclear emulsion is well expressed by dN=exp[a+k exp(η-y_b)]d[exp(η-y_b)] where the pseudorapidity is η=-ln[tan(θ/2)], the laboratory system primary rapidity is y_b, and k=-0.057+0.008. The shape of this frequency of occurrence distributions in terms of exp(η-y_b) attests to the validity of the concept of "limiting fragmentation" for helium projectile fragments produced in the projectile fragmentation regions of heavy ion collisions in nuclear emulsion.

Population structure analysis reveals the maintenance of isolated sub-populations of weedy rice

CHUNG, J-W,PARK, Y-J Blackwell Publishing Ltd 2010 Weed research Vol.50 No.6

<P><SMALL>CHUNG</SMALL> J-W & <SMALL>PARK</SMALL> Y-J (2010). Population structure analysis reveals the maintenance of isolated sub-populations of weedy rice. <I>Weed Research</I> <B>50</B>, 606–620.</P><P>Summary</P><P>This study was conducted to assess the genetic diversity, population structure and linkage disequilibrium (LD) of weedy rice in Korea using 73 weedy rice, 24 landrace lines, 12 breeding lines and 24 international varieties. In total, 772 alleles were detected in 63 microsatellite loci. The number of alleles per marker locus ranged from two to 40, with an average of 12.3. The gene diversity and polymorphism information content value averaged 0.694 and 0.668, with a range of 0.016–0.941 and 0.016–0.946 respectively. In a model-based analysis of population structure, Korean rice germplasm was classified into four subpopulations, designated ‘cultivated <I>indica</I>’, ‘cultivated <I>japonica</I>’, ‘weedy <I>indica</I>’, ‘weedy <I>japonica</I>.’ Pair-wise estimates of <I>F</I><SUB>ST</SUB> indicated different degrees of differentiation between the four model-based populations, with values ranging from 0.073 (between cultivated and weedy <I>japonica</I>) to 0.474 (between weedy <I>japonica</I> and weedy <I>indica</I>). The weedy <I>indica</I> population appeared to be highly differentiated, compared with the other populations. The cultivated <I>indica</I> lines had the highest gene diversity (0.58), followed by cultivated <I>japonica</I> (0.50), weedy <I>japonica</I> (0.42) and weedy <I>indica</I> (0.35). The total number of private alleles in weedy <I>indica</I> and <I>japonica</I> populations was 39 (23 markers) and 55 (22 markers) respectively. The average of LD (<I>r</I><SUP>2</SUP>) of weedy <I>indica</I> and <I>japonica</I> types was higher than that of the two cultivated populations, both for inter- and intrachromosome values, indicating the possible reproductive and geographical isolation of sub-populations in cultivated rice fields.</P>

이동 작동기형 인공심장의 모터부와 작동기부의 효율 분석

정진한,이정주,박찬영,김욱은,최재순,민병구 제주대학교 인공심장이식연구소 2000 인공심장 연구 Vol.1 No.2

이동 작동기형 인공 심장의 모터부와 모터부를 포함한 작동기부의 효율 측정방법과 측정 결과이다. 이동 작동기형 인공심장은 에너지 변환기로 브러시 없는 직류 모터(S/M 506-26A, Sierracin/Magnedyne, Cafsbad, CA, U.S.A.)를 사용한다. 이 브러시 없는 직류 모터가 기어 열(Gear train)을 따라 원추운동을 하며 혈액주머니에 힘을 가하게 되고, 이 힘에 의해 혈액주머니 안의 혈액이 박출하게 된다. 효율 측정을 위하여 제어기 및 모터부, 기어부 및 작동기부, 혈액주머니 밀 기타부등 크게 세 부분으로 나누었으며, 각각을 다시 소자별로 나누어 입, 출력의 효율을 측정했다. 부하의 토크, 분당 회전수, 각속도, 인가되는 전압 등을 바꾸어 가면서 시스템의 효율을 측정헌고, 이 측정을 통해서 모터 운동시의 전체적인 효율과 부분별 효율을 알 수 있다. 특히 인공심장의 모터부와 작동기부의 효율측정에 중점을 두었다. 측정 결과 4ℓ/min 박출량의 경우, 이동 작동기형 인공심장의 전체 효율은 8 %정도이며, 구체적으로는 모터부에서 50 %정도, 작동기부에서 85 %정도, 그리고 혈액주머니에서 19 %정도의 査율을 보임을 확인했다. This is a test report of motor and actuator efficiency for the moving actuator type total artificial heart. Our moving-actuator type total artificial heart, as an energy converter, utilizes a brushless DC motor(S/M 566-26A, Sierracin/Magnedyne, Carlsbad, CA, U.S.A.) generating a pendulous motion of itself by an epicyclic gear train. It is necessary to know the overall efficiency of our system. We operated the motor at a variable ranee of torque, angular speed and width of voltage pulse. Measurements of power into the drive, from the drive to the motor, and out of the motor were taken in order to derive the efficiency of the drive, efficiency of the motor and overall efficiency for the system. Throughout the test, the power input to the drive, the power output from the drive, and the power output from the motor are measured simultaneously. We focused on efficiency of the motor and actuator parts in the TAH. In case of 4 ℓ/min condition, system efficiency is about 8 %, motor part is about 50%, actuator part is about 85 %. Some experimental results are provided to demonstrate the effectiveness of the presented approach.

암에르고미터 운동시 여유심박수에서의 신체작업능력(PWC%HRR)을 이용한 최대산소섭취량 추정에 관한 연구

정성태,전태원,정덕조,정영수,엄우섭,이동기,윤호섭 한국운동과학회 2000 운동과학 Vol.9 No.1

암에르고미터 운동시 여유심박수에서의 신체작업능력(PWC%HRR)을 이용한 최대산소섭취량 측정에 관한 연구 운동과학. 제 9권 제 1호. (1)-(12), 2000. 본 연구의 목적은 암에르고미터 운동시 여유심박수에서의 신체작업능력(PWC%HRR)을 이용해 최대산소섭취량(O₂_(max))을 추정하는데 있어 그 타당성을 보는데 있다. 이를 위해 본 연구에서는 건강한20대 남자 대학생(21.1±2.77)20명이 참가 하였다. 이들의 안정시 심박수는 5분간 EKG로 측정되었고, 최대산소취량은 암에르고미터에서 50rpm의 속도로 20W에서 운동을 실시하고 매 3분 간격으로 10W씩 증가시켜 피험자가 탈진시까지 운동을 지속시켰다. 여유심박수 계산에 있어서 최대심박수는 수식(HRmax = 210 - age)으로 계산되었다. PWC%HRR의 계산은 점증부하 운동상에서 120≤R≥160의 범위에 발견된 HR과 O₂의 관계식(Y=bx+a)에 의하였다. 강도별 상대적 백분율 범위는 50∼85%사이였으며, 비교대상은 PWC%HRR, O₂R, O₂_(max)이었다. 그 결과 다음과 같은 결과를 얻었다 첫째, %HRR과 O₂R, O₂_(max)간의 회귀분석결과 %HRR은 O₂_(max)보다 O₂R에 가까웠다. 둘째, 각 강도별 PWC%HRR과 O₂R, O₂_(max)간에는 통계적으로 유의한 차이는 없었으나, PWC%HRR은 O₂_(max)보다 O₂R에 보다 가까웠다(P<.05). 셋째, O₂_(max)의 실측치와 PWC%HRR = O₂R에 의한 추정치 사이에는 모든 강도에서 통계적으로 유의한 차이가 없었으나, 상관관계는 PWC%HRR에서 가장 높았다(P<.05). 이상의 결과를 종합해 볼 때 암에르고미터 운동검사 과정에서 만족할 만한 O₂_(max) 발견하지 못했더라도 최소한 60%HRR에 해당하는 심박수가 발견될 때까지 운동을 수행하였다면 일련의 방식에 의해 O₂_(max)의 추정이 가능하다고 본다. The study on Estimation of maximal oxygun uptake through PWC%HRR during Arm Ergometer Exercise. Exercise Science, 9(1): 1-12, 2000. The purpose of this study was to evaluate the validity for estimation of maximal oxygen uptake through PWC%HRR during Arm Ergometer Exercise. The subjects of this study were 20 healthy males(21.2±2.77yrs) college students. Resting heart rate of subjects were recorded by EKG for 5min. the maximal oxygen uptake was determined until subjects were exhausted. The first stage was performed 50rpm, 20W, and then was increased by 10W every 3 minutes on the arm ergometer. HRmax was determined by a numerical formula of HRmax = 210-age. PWC%HRR was assessed by relative equation (Y=bX+a) of HR and V˙O₂ observed in range 120≤R≥160 during the exercise test. Range of relative percentage was between 50% and 85%, comparison factor was examined among PWC%HRR, % V˙O₂R and % V˙O₂_(max). The results of this study are as follows : 1) Result of the regression analysis among PWC%HRR, % V˙O₂R and % V˙O₂_(max), %HRR was equivalent to % V˙O₂R rather than V˙O₂_(max). 2) No significant difference was observed among PWC%HRR, % V˙O₂R and V˙O₂_(max) in the all intensity, But the PWC%HRR was closer to % V˙O₂R rather than V˙O₂_(max)(P<.05). 3) No significant difference was the actual V˙O₂_(max) and the estimation value by PWC%HRR = % V˙O₂R in the all intensity, and the correlation analysis was the most correlated at the estimation value by PWC%60HRR. It was concluded that if the satisfactory V˙O₂_(max) was not found in Arm Ergometer exercise test but heart rate corresponded to 60%HRR, V˙O₂_(max) can be estimated by a series of equation.