全南地方의 韓牛에 發生하고 있는 原因不明의 急性疾患에 關한 硏究(豫報)

康炳奎,金廣植,李政吉 全南大學校 農漁村開發硏究所 1973 農業科學技術硏究 Vol.7 No.-

Studies were made on the etiology of the acute, unknown disease of Korea cattle in Chonnam area. Total 12 cattle reared in the infected area were selected and examined periodically from May to November in 1972. Of the 12 cattle, two were died during the experiment. They were autopsied and histopathologically examined. In order to compare with symptoms and histopathological changes of died cattle, three goats were poisoned with phenylmercuric-triethanol-ammonium borate(PTAB), sodium nitrate(NaNO₃), and potassium cyanide(KCN). Meanwhile, samples of water, soil, and feedstuffs from the infected and noninfected area were analysed. Results obtained and matters presumed were as follows: 1. Chemical examinations for blood revealed that Korean cattle reared in the infected area had pronounced hypomagnesemia and hypocalcemia. The total leukocyte count was gradually increased in the blood examination of the two Korean cattle died during the experiment. In the analysis of soil, water, and feedstuffs of the infected and noninfected area, the differences were noted in the values of magnesium, calcium, and nitrate nitrogen. 2. Similar histopathological changes of liver and kidney in died cattle and poisoned goats led us to suspect a toxic substance. 3. Next studies should include the blood examination for calcium, magnesium, nitrate and nitrite nitrogen, cyanide, copper, vitamine A, and methemoglobin. Further investigations on soil, water, and feedstuffs are also desired.

쥐의 實驗的 葡萄狀球菌性乳房炎에 關한 硏究

李政吉,康炳奎,李採瑢 全南大學校 農漁村開發硏究所 1980 農業科學技術硏究 Vol.15 No.-

The purpose of the present experiment is to determine the suitability of rat model for the experimental staphylococcal mastitis. A strain of Staphylococcus aureus isolated from a case of bovine mastitis was inoculated through the teat canal into the mammary gland of lactating rats. The rats were removed from their offspring or left with them to allow suckling, as required by the experimental design. 1. No generalized reactions or symptoms of mastitis were observed in all rats. 2. At necropsy, affected glands showed discoloration; abscesses up to 2.5mm in diameter were observed in one group of "lactating" rats inoculated with 10x10?? colony-forming units of staphylococcus. 3. Histologically fat droplets retained within the alveoli were prominent in many sections. Epithelial hyperplasia and slight interalveolar fibrosis were also noted. 4. The number of bacteria recovered from the inoculated mammary gland was smaller than that of bacteria inoculated. The bacteria were more readily recoverable from the mammary glands of "non-lactating" rats than from "lactating" rats. 5. To determine a suitable rat model for the experimental staphylococcal mastitis, further studies should be carried out, using different strains of Staphylococcus aureus and also different strains of rats.

Pipe 형상의 입자강화형 금속 복합재료 부품의 Thixocasting 디지털 생산기술

허재찬,강충길 한국주조공학회 2001 한국주조공학회지 Vol.21 No.6

變質된 옥수수겨의 給與로 因한 乳牛의 中毒症例

羅鎭洙,李政吉,康炳奎 全南大學校 農漁村開發硏究所 1979 農業科學技術硏究 Vol.14 No.-

An outbreak of toxicity syndrome occurred in 17 Holstein cows after eating moldy corn bran in June, 1979. Three of 17 cows died, nine were killed owing to being unable to rise and five recovered after showing signs of illness The clinical signs of illness were hyper-excitability, muscular spasms, shaking or stamping with rear limbs, rigors, ataxia, recumbency, anorexia, non-rumination, diarrhea, polyuria, loss of condition, rapid fall in milk production, dyspnea, fever and abortion. Necropsy findings included pulmonary hyperemia or emphysema, intestinal hemorrhages, edematous kidneys, and intra-uterine hemorrhages. Histopathologicl findings showed hemorrhages and congestion of spleen, dilated sinusoids and pyknosis of hepatocytes of liver, pulmonary emphysema and interstitial hyperplasia and hemorrhages into the renal tubules. The suspected moldy corn bran proved to be toxic by experimental feeding to mice. From the above-mentioned results, it is suspected that the cause of the outbreak was mycotoxicosis. Results of analysis of the feed for toxic agents and identification of the mold will be reported later.

정신분열병과 N-Methyl-D-Aspartate 수용체 유전자와 연관된 VNTR 표지자인 D9S158 다형성의 연합

김종원,채영규,정은기,김길숙,박강규,이영호,최인근,양병환,한진희 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.5

정신분열병과 NMDA 수용체와 연관된 D9S158 유전적 표지자와의 연관성을 알아보기 위해 75명의 정신분열병 환자군과 87명의 정상대조군을 대상으로 중합효소연쇄반응을 이용하여 연합연구를 시행하여 다음과 같은 결론을 얻었다. D9S158 유전좌위에는 모두 8개의 대립유전자가 있었고, 각 대립유전자는 cytosine-adenine염기가 반복되는 217염기쌍에서 233염기쌍의 형태였다. 먼저 정신분열병 환자와 정상대조군의 대립유전자의 빈도를 비교해 본 결과 통계적으로 유의한 차이를 보이지 않았다(p〉.05). 환자군을 좀 더 동질적으로 만들기 위해 정신분열병의 가족력 유무, 음성 및 양성증상, 연성신경학적 징후, DSM-IV 임상아형에 따라 환자군을 나누어 대립유전자의 빈도를 비교해 본 결과, 특정 임상양상과 관련이 있는 대립유전자를 발견하지 못하였다. 결론적으로 본 연구에서는 D9S158 유전좌위와 정신분열병의 연관성을 입증하지 못하였으나 NMDA 수용체 유전자주위의 다른 표지자나 좀 더 유전적으로 동질한 환자군을 대상으로 한 추후연구가 필요할 것으로 생각된다. An association study with Korean schizophrenic patients(N=75) and normal controls(N=87) was performed to find the relationship between D9S158 polymorphism and schizophrenia using polymerase chain reaction. Eight different alleles of a dinucleotide polymorphism on D9S158 locus were observed in both group. When we compared the frequencies of alleles between schizophrenics and normal controls, there was no significant difference between two groups. To increase homogeneity of schizophrenic group, we divided schizophrenic group by clinical phenotypes such as family history, negative and positive symptoms(PANSS), soft neurologic signs(NES-K) and DSM-IV diagnostic subtypes. Then we compared the frequencies of alleles among subgroups of clinical phenotypes, and there were no significant differences between subgroups(p〉.05). Although our findings fail to provide an evidence of association between schizophrenia and D9S158 locus, further investigation of other loci that are linked to NMDA receptor gene may be needed in genetically homogeneous subgroups of schizophrenia.

혈액투석 환자에게서 발생한 재생불량성 빈혈 1예

정지용,윤나라,강대웅,오종찬,장재현,선길홍,박경희,정종훈,김현리 朝鮮大學校 附設 醫學硏究所 2005 The Medical Journal of Chosun University Vol.30 No.1

Aplastic anemia is a disease characterized by pancytopenia in the peripheral blood and hypocellular bone marrow with the fatty infiltration. The causes of the acquired aplastic anemia were usually related to drugs and immune-related diseases. A 34-years old man was admitted due to general weakness. He has been going hemodialysis for 2 years because of end stage renal disease (ESRD) due to chronic glomerulonephritis. At admission, laboratory tests showed hemoglobin 6.2 g/dL, hematocrit 18.0 %, WBC 5,710mm (neutrophil 71.8%, lymphocyte 19.1%, monocyte 5.9%), reticulocyte 1.9%, platelet 93,000/mm, Fe 12.8 ug/dL, TIBC 204 ug/dL, ferritin 941.47 ng /ml, haptoglobin 0.72 g/L, vitamin B12 508.17 pmol/L, folate 24 ng/mL, total protein 6.54 g/dL, albumin 3.76 g/dL, Alk. pohsphatase 79 IU/L, AST 30.0 IU/L, ALT 39.7 IU/L, total bilirubin 0.57 mg/dl, BUN 79.4 mg/dl and creatinine 10.18 mg/dl. Peripheral blood smear showed the normocytic normochromic anemia with anisocytosis. Anemia was diagnosed but it was irresponsive to the recombinant human erythropoietin (EPO) and blood transfusion. In the bone marrow aspiration smear were there the hypoplastic myeloid and erythroid precursors. The bone marrow biopsy section showed the hypoplasia of all components (10-20%) and the fatty infiltration. We have experienced one case of the idiopathic aplastic anemia in a patient going through hemodialysis and we just report it with documentary records. 저자들은 혈액 투석 중인 환자에서 약물, 면역계 이상등의 원인을 발견할 수 없었음에도 재생 불량성 빈혈이 발생한 1예를 경험하여 문헌 고찰과 함께 보고하는 바이다.

慶北地域에서 栽培中인 芍藥의 Paeoniflorin 含量分析

이인구,주길재,김태강,정재동 慶北大學校農業科學技術硏究所 1996 慶北大農學誌 Vol.14 No.-

主재배품종인 의성종과 영천종의 유효성분을 HPLC 및 TLC로 분석한 결과 主성분인 paeoniflorin 함량은 의성종이 영천종보다 높으며 albiflorin과 oxypaeoniflorin의 함량을 두 재배종에서 비교하면 의성종은 albiflorin 함량이 많고 영천종은 oxypaeoniflorin의 함량이 많았다. 생산지에 따른 paeoniflorin의 함량차이는 2.15%에서 4.08%의 범위였고 의성과 거창의 것은 비슷하였으며 영천의 것이 가장 낮았다. 18종의 재배종들은 품종에 따라 다양한 paeoniflorin 함량 차이를 보이며 작약성분분석의 HPLC 크로마토그람을 albiflorin, oxypaeoniflorin 및 paeoniflorin피크를 기준으로 비교해본 결과 크게 3가지 패턴으로 분류할 수 있었다. 이 세가지 유형은 의성종 패턴 9종, 영천종 패턴 4종 풍기표준 종패턴 5종 이었다. 수확시기에 따른 작약의 paeoniflorin의 함량변화를 조사한 결과 3월과 9월에 수확한 것이 낮았으며 5월과 11월에 수확한 작약의 paeoniflorin 함량이 높았다. The effective components of the peony cultivar, Euseongjagyag and Youngcheonjagyag, which were the major cultivars of peony in Kyeongbuk area, were determined with HPLC and TLC. The paeoniflorin content in the root of Euseongjagyag were more than that of Youngcheonjagyag. The root of Euseongjagyag contained much albiflorin and Youngcheonjagyag contained much oxypaeoniflorin in comparision with albiflorin and oxypaeoniflorin contents in both cultivars of peony. Paeoniflorin contents in accordance with peony prodution regions were ranged from 2.15% to 4.0%, and paeoniflorin content of local cultivar of Euseong and that of Geochang were approximatly the same but that of Youngcheon was the lowest. Paeoniflorin content in the 18 accessions of peony cultivar which were collected from Kyeongbuk area and harvested on November 1993, were ranged from 1.41% to 5.30%. The 18 accessions of peony were classified with the HPLC chromatogram pattern of peony root extract into the three groups which composed with Euseong peony group(9 accessions), Youngcheon peony group (4 accessions) and Punggi standard peony group(5 accessions). High content of paeoniflorin was contained in peony root harvested in May and November, but low content of paeoniflorin was contained in peony root harvested in March and September.

감의 脫澁處理中 Pectin 含量과 Texture의 變化

崔鍾旭,孫泰華,鄭信敎,李吉雨,姜峻洙 慶北大學校 1989 論文集 Vol.47 No.-

This experiment was made to investigate the changes of the contents of acetaldehyde, alcohol, tannin, and to study the texture changes of persimmon in relation to the contents of pection during the removal of astringency by CO_2. 1. During the removal of astringency of persimmon fruits, the contents of soluble tannin showed the rapid decrease in high concentration of CO_2, but the contents of ethanol and acetaldehyde showed the rapid increase in all treatments. 2. The contents of W.S.P. increased, but H.S.P. decreased in all treatments. 3. All texture paramenters of persimmon fruits except springness and adhesiveness were decreased druing the removal of astringency. 4. The contents of W.S.P. were good correlated with the changes of hardness in all treatments.

주편 연속주조시 Thermal Soft Reduction 적용에 의한 중심 편석 개선

정영진,김지준,김선구,강충길 대한금속재료학회 2003 대한금속·재료학회지 Vol.41 No.11

Centerline macrosegregation is one of the most significant problems in continuous casting on stainless steel slabs. Numerical model have been developed in order to decrease centerline macrosegregation during continuous casting of STS 420J2 stainless steel slab. Extra cooling zone and extra cooling water quantity are determined by iterative coupled analysis of heat transfer and segregation. Segregation ratio is decreased by the intensive cooling at the extra cooling zone above the solid fraction of the slab center 0.7, so called thermal soft reduction (TSR). The experimental results show good agreements with numerical results.

Determination of Die Design Rules for Semi-Solid Die Casting Process and Its Experimental Investigation

Kang, Chung Gil,Seo, P.K.,Kim, Byung Min Trans Tech Publications, Ltd. 2005 Materials science forum Vol.475 No.-

<P>Die design rule for semi-solid die casting (SSDC) with A356 electromagnetic stirring (EMS) aluminum alloy, was proposed. The die design rule included inspection of machine, part requirements, parting line determination, sleeve, plunger, gating system, overflow, air vent, ejector pin, and heating line design. The specification of gating system, overflow, air vent, plunger tip, and sleeve suitable for respective part were regulated. Two steps die system of lower-positioned gate and three steps die system of center-positioned gate were manufactured for 4 automobile suspension parts, based on the die design rule. For the sound filling pattern and solidification behavior, injection speeds of 4 parts were summarized to the interval (from V1 to V4). As a result of observing the microstructure of 4 parts after T6 heat treatment, primary Al-α phase was globularized and fine Si particles were distributed around the grain boundary. The mechanical properties of 4 parts with T6 heat treatment were investigated and showed ultimate tensile strength (UTS) of 330 MPa, yield strength (YS) of 250 MPa, and elongation of 7.5% as average.</P>