Systematic Review of Single Large and/or Multinodular Hepatocellular Carcinoma: Surgical Resection Improves Survival

Yang, Xiang-Di,Pan, Ling-Hui,Wang, Lin,Ke, Yang,Cao, Ji,Yang, Chun,Zhong, Jian-Hong,Luo, Wang,Guo, Jiao,Li, Le-Qun Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.13

Background: The role of surgical resection for patients with single large (${\geq}5cm$) and/or multinodular (${\geq}2$) hepatocellular carcinoma (HCC) is still controversial. This systematic review was performed to evaluate the safety and efficacy of resection for patients with single large and/or multinodular HCC. Materials and Methods: Databases (the PubMed, Web of Science, Embase, and Cochrane databases) were systematically searched to identify relevant studies exploring the safety and efficacy of resection for single large and/or multinodular HCC, published between January 2000 and December 2014. Perioperative morbidity and mortality, overall survival, and disease-free survival of the resection group were calculated. In addition, these outcome variables were also calculated for the control group in the included studies. Results: One randomized controlled trial and 42 nonrandomized studies involving 9,580 patients were eligible for analysis. Eight (1,594 patients) of the 43 studies also reported the outcomes of transarterial chemoembolization (TACE). Although 51.4% of patients featured cirrhosis, 90.7% of them demonstrated Child-Pugh A liver function in the resection group. The median rates of morbidity (24.5%) and mortality (2.5%) after resection were significantly higher than that of TACE (11.0%, P<0.001; 1.9%, P<0.001). However, patients who underwent resection had significantly higher median one-, three-, and five-year overall survival (76.1%, 51.7%, and 37.4%) than those who underwent TACE (68.3%, 31.5%, and 17.5%, all P<0.001). The median 1-, 3-, and 5-year DFS rates after resection were 58.3%, 34.6%, and 24.0%, respectively. Conclusions: Although tumor recurrence after resection for patients with single large and/ or multinodular HCC continues to be a major problem, resection should be considered as a strategy to achieve long-term survival.

Complete Genome Sequence of Salmonella enterica Serovar Pullorum Multidrug Resistance Strain S06004 from China(s)

( Qiu Chun Li ),( Ya Chen Hu ),( Yin Fei Wu ),( Xiao Chun Wang ),( Xiao Lei Xie ),( Ming Xin Tao ),( Jun Lei Yin ),( Zhi Jie Lin ),( Yang Jiao ),( Li Juan Xu ),( Xinan Jiao ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.5

As Salmonella enterica serovar Pullorum remains a major economic problem for the poultry industries of countries with no efficient control measures, we presented a multidrug resistance strain S06004 (isolated from a clinically sick chicken in China in 2006) for genome sequencing. The genome comparison showed that the strain contained two prophages, the ST104 and prophage-4 (Fels2) of E. coli LF82, which were not detected in the only published genomes of S. Pullorum RKS5078 and CDC1983-67. In addition, the GyrA Ser83 point mutation, drugresistant genes, and many antibiotic pump systems that are present in S06004 may be contributing to the multidrug resistance of this strain.

Enhancement of Haloacetate Dehalogenase Production by Strain Mutation and Condition Optimization

Chun-jiao Lin,Li-rong Yang,Gang Xu,Jian-ping Wu 한국생물공학회 2011 Biotechnology and Bioprocess Engineering Vol.16 No.5

Enhancement of the activity of an inducible chloroacetate dehalogenase was carried out by efficient and safe mutation with UV and microwave irradiation along with optimization of culture conditions. First, a stable mutant of Pseudomonas sp. CGMCC 3267-MW6 with chloroacetate dehalogenase activity of 2.77 U/mL (3-fold higher activity than the wild strain) was produced by mutation. The maximum activity of this inducible enzyme was measured as 29.41 U/mL when Pseudomonas sp. CGMCC 3267-MW6 was cultured with 4 g/L 3-hydroxybutyrate for 12 h followed by 40 mM 3-chlorobutyrate for an additional 20 h. Production of the enzyme was found to be associated with growth of the bacterium. According to these results, we determined that the optimum inducer of chloroacetate dehalogenase activity would be a hard degradable substrate. The optimum auxiliary carbon source would be the primary metabolite of the substrate or the precursor of the metabolite. The optimum time of inducer supplementation would be during the middle stage of exponential phase. The optimum concentration of substrate would be sufficient but would not induce inhibition. Finally, the optimum collection time would be at the later stage of exponential phase. This work provides further knowledge of chloroacetate dehalogenase and the optimization of inducible enzyme production.

Case-control study on the fibroblast growth factor receptor 2 gene Polymorphisms associated with breast cancer in in Chinese Han women

Chun-Lian Liu,Xiao-Ping Hu,Wei-Dong Guo,Li Yang,Jie Dang,Hai-Yan Jiao 한국유방암학회 2013 Journal of breast cancer Vol.16 No.4

Purpose: Genetic variation in fibroblast growth factor receptor 2(FGFR2) is a newly described risk factor for breast cancer. Thisstudy aimed to evaluate the association of four single nucleotidepolymorphisms (SNPs) in FGFR2 with breast cancer in Han Chinesewomen. Methods: Two hundred three women with breastcancer and 200 breast cancer-free age-matched controls wereselected. Four SNPs (rs2981579, rs1219648, rs2420946, andrs2981582) and their haplotypes were analyzed to test for theirassociation with breast cancer susceptibility. The presence ofthe four FGFR2 SNPs was determined by polymerase chain reaction-restriction fragment length polymorphism analysis. Results:A statistically significant difference was observed in thefrequency of rs2981582 in the FGFR2 gene (p<0.05) betweencase and control groups. In subjects stratified by menopausalstatus, rs2981582 TT, rs2420946 AA, and rs1219648 CC weresignificantly associated with the risk of breast cancer in postmenopausalsubjects, but no significant associations betweenthese four SNPs and the risk of breast cancer were identified inpremenopausal subjects. Further, there was no significant associationbetween hormone receptor status (estrogen receptor andprogesterone receptor) and breast cancer risk. Six common (>3%) haplotypes were identified. Three of these haplotypes,CGTC (odds ratio [OR], 0.613; 95% confidence interval [CI],0.457-0.82; p=0.001), TGTC (OR, 6.561; 95% CI, 2.064-20.854;p<0.001), and CATC (OR, 12.645; 95% CI, 1.742-91.799; p=0.001) were significantly associated with breast cancer risk. Conclusion:Our findings indicated that the SNP rs2981582 and haplotypesCGTC, TGTC, and CATC in FGFR2 may be associatedwith an increased risk of breast cancer in Han Chinese women.

臨床觀察關于愈肝汤治療濕热型肝病

謝春嬌(Chun Jiao Xie),徐富一(Bu Il Seo),宋春浩(Choon Ho Song),楊裕仁(Yoo In Yang),申舜植(Soon Shik Shin) 한의병리학회 2008 동의생리병리학회지 Vol.22 No.3

Tumor Necrosis Factor-α Gene Polymorphisms and Risk of Oral Cancer: Evidence from a Meta-analysis

Chen, Fang-Chun,Zhang, Fan,Zhang, Zhi-Jiao,Meng, Si-Ying,Wang, Yang,Xiang, Xue-Rong,Wang, Chun,Tang, Yu-Ying Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.12

Numerous studies have been conducted regarding association between TNF-${\alpha}$ and oral cancer risk, but the results remain controversial. The present meta-analysis is performed to acquire a more precise estimation of relationships. Databases of Pubmed, the Cochrane library and the China National Knowledge Internet (CNKI) were retrieved until August 10, 2013. Pooled odds ratios (ORs) and 95% confidence intervals (95% CIs) were calculated with fixed- or random-effect models. The heterogeneity assumption was assessed by I-squared test. Among the eight included case-control studies, all were focused on TNF-${\alpha}$-308G>A and four also concerned the TNF-${\alpha}$-238G>A polymorphism. It was found that oral cancer risk were significant decreased with the TNF-${\alpha}$-308G>A polymorphism in the additive genetic model (GG vs. AA, OR=0.19, 95% CI: [0.04, 1.00], P=0.05, I2=68.9%) and the dominant genetic model (GG+GA vs. AA, OR=0.22, 95% CI: [0.06, 0.82], P=0.03, I2=52.4%); however, no significant association was observed in allele contrast (G vs. A, OR=0.70, 95% CI: [0.23, 2.16], P=0.54, I2=95.9%) and recessive genetic models (GG vs. GA+AA, OR=0.72, 95% CI: [0.33, 1.57], P=0.41, I2=93.1%). For the TNF-${\alpha}$-238G>A polymorphism, significant associations with oral cancer risk were found in the allele contrast (G vs. A, OR=2.75, 95% CI: [1.25, 6.04], P=0.01, I2=0.0%) and recessive genetic models (GG vs. GA+AA, OR=2.23, 95%CI: [1.18, 4.23], P=0.01, I2=0.0%). Conclusively, this meta-analysis indicates that TNF-${\alpha}$ polymorphisms may contribute to the risk of oral cancer. Allele G and the GG+GA genotype of TNF-${\alpha}$-308G>A may decrease the risk of oral cancer, while allele G and the GG genotype of TNF-${\alpha}$-238G>A may cause an increase.

Changes in Volatile Aroma Compounds of Traditional Chinese-type Soy Sauce During Moromi Fermentation and Heat Treatment

Xian-Li Gao,Chun Cui,Hai-Feng Zhao,Mou-Ming Zhao,Lan Yang,Jiao-Yan Ren 한국식품과학회 2010 Food Science and Biotechnology Vol.19 No.4

Considering the important influence of longtime (150 day) moromi fermentation and heat treatment on the aroma formation of traditional Chinese-type soy sauce (TCSS), volatile compounds in samples taken from different stages of moromi fermentation and heat treatment were analyzed by solid phase microextraction coupled with gas chromatography-mass spectrometry. Results showed that a total of 76 volatile compounds were identified in all the samples, and most of the volatile compounds were common. During 150 day of moromi fermentation, relative contents of acids, alcohols, aldehydes and ketones, esters,and furan(one)s along with all the sensory attributes of acidic, alcoholic, fruity, caramel-like, smoky, and malty changed greatly. Notably, relative contents of alcohols,aldehydes and ketones along with the sensory intensities of alcoholic, caramel-like, and smoky of heated sample (80℃/60 min) decreased markedly, whereas there were slight increases in relative contents of furan(one)s, phenols,and sulfur-containing compounds of it. Long-time moromi fermentation and heat treatment have significant influence on the formation and relative contents of volatile compounds in TCSS, whereas changes in volatile compounds and their relative contents of the samples were responsible for the differences in sensory attributes.

High Expression of Forkhead Box Protein C2 is Related to Poor Prognosis in Human Gliomas

Wang, Yao-Wu,Yin, Chun-Li,Zhang, Hong-Yi,Hao, Jin-Min,Yang, Yue-Ye,Liao, Heng,Jiao, Bao-Hua Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.24

Background: Increasing evidence has indicated that high Forkhead box protein C2 (FOXC2) level is closely associated with the development, progression, and poor prognosis of a variety of tumors. However, the relationship between FOXC2 and the progression of human gliomas remains to be clarified. The aim of present study was to assess FOXC2 expression and to explore its contribution in human gliomas. Materials and Methods: Realtime quantitative PCR was performed to examine FOXC2 expression in 85 pairs of fresh frozen glioma tissues and corresponding non-neoplastic brain tissues. Associations of FOXC2 expression with clinicopathological factors and prognosis of glioma patients were statistically analyzed. Results: The relative mRNA expression of FOXC2 was significantly higher in glioma tissues than the corresponding non-neoplastic brain tissues (p<0.001). In addition, high FOXC2 expression was significantly associated with advanced pathological grade (P=0.005) and the low Karnofsky performance score (KPS) (p=0.003), correlating with poor survival (p<0.001). Furthermore, multivariate Cox regression analysis showed that high FOXC2 expression was an independent predictor of overall survival (p=0.006). Conclusions: FOXC2 may act as an oncogenic gene and represent a potential regulator of aggressive development and a candidate prognostic marker in human gliomas.

Implementation of Non-Local Multi-Qubit CNOT Operation with Multi-Qubit GHZ States

Yan-Hui Zhou,Xiao-Qiang Shao,Chun-Jiao Yang,Shou Zhang,연규황 한국물리학회 2008 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.53 No.2

A scheme is proposed to implement a non-local controlled-not (CNOT) operation with two control qubits and two target qubits, where two three-particle Greenberger-Horne-Zeilinger (GHZ) states and eight cbits (bits of classical communication) are needed. The scheme can also be generalized straightforwardly to implement a non-local multi-qubit operation with M control qubits and N target qubits.

Growth and Differentiation Effects of Homer3 on a Leukemia Cell Line

Li, Zheng,Qiu, Hui-Ying,Jiao, Yang,Cen, Jian-Nong,Fu, Chun-Mei,Hu, Shao-Yan,Zhu, Ming-Qing,Wu, De-Pei,Qi, Xiao-Fei Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.4

The Homer protein family, also known as the family of cytoplasmic scaffolding proteins, which include three subtypes (Homer1, Homer2, Homer3). Homer3 can regulate transcription and play a very important role in the differentiation and development for some tissues (e.g. muscle and nervous systems). The current studies showed that Homer3 abnormal expression changes in acute myeloid leukemia (AML). Forced expression of Homer3 in transfected K562 cells inhibited proliferation, influenced the cell cycle profile, affected apoptosis induced by $As_2O_3$ through inhibition of Bcl2 expression, and also promoted cell differentiation induced by 12-O-tetra decanoylphorbol-acetate (TPA). These results showed that Homer3 is a novel gene which plays a certain role in the occurrence and development of AML.