Expression of Smad7 in Cholangiocarcinoma: Prognostic Significance and Implications for Tumor Metastasis

Huang, Qiang,Liu, Lei,Liu, Chen-Hai,Shao, Feng,Xie, Fang,Zhang, Chuan-Hai,Hu, San-Yuan Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.10

Background: There are few molecular markers known to predict cholangiocarcinoma (CCA) prognosis. Smad7 has a certain relationship with epithelial-mesenchymal transition (EMT), but its relevance to CCA in unclear. Therefore expression and clinical significance of Smad7 in CCA was the focus of this study. Methods: Expression of Smad7, E-cadherin and vimentin was assessed in 41 patients with CCA by immunohistochemistry and analyzed for associations with clinical parameters. Results: Smad7 and vimentin expression in the CCA tissue was dramatically higher than that in adjacent tissues. In addition, Smad7, vimentin and E-cadherin expression was significantly associated with CCA lymph node metastasis and perineural invasion($P{\leq}0.05$), but not other factors, such as gender, age, tumor location, tumor type and tumor differentiation degree (P>0.05). The overall survival and relapse-free survival rate was significantly higher in patients with negative Smad7 expression than those with positive Smad7 expression. Conclusion: EMT phenomena may occur in the process of CCA invasion and metastasis. Smad7, which was highly expressed in CCA, may be considered to be one feedback regulator in late stages and could have potential as a prognostic indicator for clinical assessment.

A Smart Manufacturing Solution for Multi-Axis Dispenser Motion Planning in Mixed Production of Shoe Soles

Yuan-Ping Luh,Liao-Chuan Huang,Hao-Jan Lu,Chih-Hsing Chu 한국정밀공학회 2020 International Journal of Precision Engineering and Vol.7 No.3

Mixed production is a practical solution to realize the idea of mass product customization. The production system needs to adapt to raw materials of product variants appearing in the production line, and thus to properly complete the manufacturing task. Such system autonomy is a core competence in smart manufacturing. This paper presents a smart manufacturing solutionfor shoe sole gluing in mixed production environment. The solution automatically guides a 5-axis CNC dispensing machine to perform the gluing task of a sole randomly placed on the worktable without use of fi xturing. A single RGB-D camera is used to capture the color and depth data of the work environment. A two-stage algorithm is implemented to provide the intelligence required by the solution using the captured data as input. The LINEMOD method fi rst determines a rough pose from training data of all shoe soles at pre-defi ned orientations. A trimmed interactive closest point (ICP) method then refi nes the pose using the rough one as the initial solution. Pre-determined working paths are transformed into the correct location around the sole with the refi ned pose. The corresponding motion commands drive the dispenser controller to complete the gluing task without collision. Test results of real shoe soles validate the eff ectiveness of the proposed solution. This work contributes to smart manufacturing in the shoe making industry, which has been less studied in the past.

MicroRNA-21 Regulates the Invasion and Metastasis in Cholangiocarcinoma and May Be a Potential Biomarker for Cancer Prognosis

Huang, Qiang,Liu, Lei,Liu, Chen-Hai,You, Hao,Shao, Feng,Xie, Fang,Lin, Xian-Sheng,Hu, San-Yuan,Zhang, Chuan-Hai Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.2

Background: MicroRNAs are noncoding RNA molecules that posttranscriptionally regulate gene expression. The aim of this study was to determine the role of microRNA-21 in cholangiocarcinomas and its relationship to cholangiocarcinoma RBE cell capacity for invasion and metastasis. Methods: MicroRNA-21 expression was investigated in 41 cases of cholangiocarcinoma samples by in situ hybridization and real-time PCR. Influence on cholangiocarcinoma cell line invasion and metastasis was analyzed with microRNA-21 transfected cells. In addition, regulation of reversion-inducing-cysteine-rich protein with kazal motifs (RECK) by microRNA-21 was elucidated to identify mechanisms. Results: In situ hybridization and real-time quantitative PCR results for patients with lymph node metastasis or perineural invasion showed significantly high expression of microRNA-21 (P<0.05). There was a dramatic decrease in cholangiocarcinoma cell line invasion and metastasis ability after microRNA-21 knockdown (P<0.05). However, overexpression significantly increased invasion and metastasis (P<0.05). Real-time PCR and Western-blot analysis showed that microRNA-21 could potentially inhibit RECK expression in RBE cells. Survival analysis showed that patients with higher expression levels of microRNA-21 more often had a poor prognosis (P<0.05). Conclusions: MicroRNA-21 may play an important role in cholangiocarcinoma invasion and metastasis, suggesting that MicroRNA-21 should be further evaluated as a biomarker for predicting cholangiocarcinoma prognosis.

Strain Rate Effects on the Mechanical Properties of an AlCoCrFeNi High-Entropy Alloy

Chuan Ting Wang,Yong He,Zhiping Guo,Xiaohui Huang,Ying Chen,Houan Zhang,Yuan He 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.7

The efect of strain rate on the compressive properties of an AlCoCrFeNi high-entropy alloy (HEA) was investigated. Microstructure of the AlCoCrFeNi HEA was observed using scanning transmission microscopy (SEM) and transmission electronmicroscopy. The results showed formation of a homogeneous two-phase structure. Quasi-static compression was testedunder initial engineering strain rates between 10–4 and 10–2 s−1. Engineering compression stress of exceeding 2500 MPaand ductility of around 12% was achieved. Dynamic mechanical behavior at room temperature was characterized by a splitHopkinson pressure bar under strain rates between 1350 and 4000 s−1. The AlCoCrFeNi HEA exhibited high strain ratesensitivity, especially under dynamic compression. The fuctuation of yield strength and the variation of strain rate hardening with strain rate was investigated. The various parameters of a constitutive equation for deformation of the AlCoCrFeNiHEA were obtained from the experimental data. The constitutive equations can be applied to predict the strength of alloyunder various stain rates.

Distinct Inflammation Biomarkers in Healthy Individuals and Patients with Schizophrenia: A Reliability Testing of Multiplex Cytokine Immunoassay by Bland-Altman Analysis

Ta-Chuan Yeh,Hsuan-Te Chu,Chia-Kuang Tsai,Hsin-An Chang,Fu-Chi Yang,San-Yuan Huang,Chih-Sung Liang 대한신경정신의학회 2019 PSYCHIATRY INVESTIGATION Vol.16 No.8

Objective Since the inflammatory process has been implicated in the pathophysiology of psychiatric disorder, an important issue emerging is to assess the test-retest reliability of cytokine measurement in healthy individuals and patients with schizophrenia. The objective of the present study was to investigate the test-retest reliability of bead-based multiplex immunoassay technology (BMIT) for cytokine measurement by using a Bland-Altman plot (BAP). Methods Twenty healthy individuals and twenty patients with schizophrenia were enrolled, and a 17-plex cytokine assay was used to measure inflammatory biomarkers at baseline and two weeks later. The test-retest reliability was examined by BAP, 95% limits of agreement (LOA), intraclass correlation coefficient (ICC), and coefficient of repeatability (CoR). Results In the healthy controls, only interleukin (IL)-2, IL-13, IL-10, IL-17, and macrophage inflammatory protein-1β showed excellent ICC. The BAP with 95% LOA determined that 13 cytokines showed acceptable 95% LOA for a 2-week test-retest reliability, and only IL-1β, IL-12 and tumor necrosis factor (TNF)-α had significant test-retest bias. The CoR of cytokines varied significantly, ranging from 1.72 to 218.1. Compared with healthy controls, patients with schizophrenia showed significantly higher levels of IL-5, IL-13, and TNF-α and significantly lower levels of IL-4, IL-12, and interferon-gamma (IFN-γ). Of these six cytokines, IL-12 and TNF-α were considered suboptimal reliability. Conclusion The findings from ICC and CoR implied that the test-retest reliability of BMIT for cytokine measurement were suboptimal. However, the BAP with 95% LOA confirmed that BMIT can reliably distinguish schizophrenia from healthy individuals in cytokine measurement, while significant within-subject variation and between-group overlapping were evident in cytokine expression.

網絡化制造中的循環經濟與跨文化管理新範式

?肖?(Xiao-yuan Yang),黃?坤(Chuan-kun Huang) 한국산업정보학회 2007 한국산업정보학회 학술대회논문집 Vol.2007 No.3

The Effects of Chicken Leg Bone Extract on Antioxidative Properties under Different Heating Condition

Cheng, Fu-Yuan,Wan, Tien-Chun,Huang, Chao-Wei,Tominaga, Kana,Lin, Liang-Chuan,Sakata, Ryoichi Asian Australasian Association of Animal Productio 2008 Animal Bioscience Vol.21 No.12

The aim of this study was to extract chicken leg bone, which is a by-product of industrial poultry processing, using different heating temperatures (80, 90 and $100^{\circ}C$) and durations (5, 10 and 15 min). The pH value, soluble protein content, peptide content and antioxidative properties, including superoxide anion scavenging ability, 1, 1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging ability, reducing capacity and inhibitory activity of linoleic acid peroxidation, were measured. The results showed no significant differences (p>0.05) in pH value among all treatments. Decreased soluble protein content and peptide content were observed in chicken leg bone extract obtained under higher heating temperatures (90 or $100^{\circ}C$) and longer heating durations (10 or 15 min). In antioxidative properties, the extracts which were heated at 90 or $100^{\circ}C$ for 15 min exhibited significantly higher superoxide anion scavenging ability, DPPH free radical scavenging ability, reducing capacity and inhibitory activity of linoleic acid peroxidation (p<0.05).

Association of a Single Nucleotide Polymorphism in the 5'-Flanking Region of Porcine HSP70.2 with Backfat Thickness in Duroc Breed

Chen, Ming-Yu,Huang, San-Yuan,Lin, En-Chung,Hseu, Tzong-Hsiung,Lee, Wen-Chuan Asian Australasian Association of Animal Productio 2003 Animal Bioscience Vol.16 No.1

Higher environmental temperature affects the economic performance of pigs. Heat shock protein 70 has been shown to play an important role in thermoresistance. The purpose of this study was to assess the effect of a single nucleotide polymorphism in the 5'-flanking region of porcine HSP70.2 on growth performance in Taiwanese Duroc. The genotype of this nt 393 polymorphic site could be verified by digestion with Bsa WI restriction enzyme of a PCR product. Pigs with TT and TC genotypes have thinner backfats than those with CC type (p<0.05). The result suggested that the polymorphic Bsa WI site in the 5'flanking region of porcine HSP70.2 may be used as a marker for the early selection of ultrasonic backfat thickness in Duroc pigs.

Generalized Anxiety Disorder, Comorbid Major Depression and Heart Rate Variability: A Case-Control Study in Taiwan

Hsin-An Chang,Chuan-Chia Chang,Nian-Sheng Tzeng,Terry B. J. Kuo,Ru-Band Lu,San-Yuan Huang 대한신경정신의학회 2013 PSYCHIATRY INVESTIGATION Vol.10 No.4

Objective Decreased heart rate variability (HRV) has been reported in generalized anxiety disorder (GAD), but the results are mixed. Little is known about the impact of comorbid major depression (MD) on HRV in GAD patients. Both issues necessitate further investigation. Methods Twenty unmedicated, physically healthy GAD patients, 20 GAD patients with a secondary diagnosis of MD, 40 MD patients and 60 matched controls were recruited. We used the Hamilton Anxiety Rating Scale and the Hamilton Depression Rating Scale to assess anxiety and depression severity, respectively. Cardiac autonomic function was evaluated by measuring HRV parameters. Frequency-domain indices of HRV were obtained. Results Three patient groups had more anxiety and depression symptoms than control subjects, but heart rates (HRs) were significantly elevated only in GAD patients with comorbid depression. Relative to controls, GAD patients had reduced HRV while GAD patients with comorbid depression displayed the greatest reductions in HRV among three patients groups. Correlation analyses revealed anxiety/depression severity significantly associated with HRs, variance, LF-HRV and HF-HRV. However, separately analyzing among individual groups and adjusting for HRV-associated covariables rendered the correlations non-significant. Conclusion Our results suggest that reduction in HRV is a psychophysiological marker of GAD and individuals with comorbid GAD and MD may be distinguished based on psychophysiological correlates (for example, HF-HRV) from non-comorbid GAD patients. Taken into account that comorbid depression may confer increased risks for cardiovascular events in GAD patients, this subgroup of GAD patients may benefit better from cardiovascular risk reduction strategies.

Fabrication and Study of Novel Piezoresistive Polymer Composites Sensor for 3D Printing Applications

( Yu-yen Chen ),( Cheng-chun Huang ),( Ching-yuan Su ),( Yao-chuan Tsai ) 한국농업기계학회 2018 한국농업기계학회 학술발표논문집 Vol.23 No.1

With the technology development, the technique of 3D printing has gradually matured. The characteristic of 3D printing is that it can print the product directly without another complex process. If the 3D printing could be applied to manufacturing sensor elements, it could be quicker and cheaper for the sensor element fabrication and the sensor forms will be applicable. Piezoresistive effect is a common sensor sensing principle which is through the deformation of the material to cause the resistance changes. In this study, a piezoresistive polymer composites material was developed, which can be manufactured by the photo-curable 3D printing. The different amount of the conductive carbon black (CB) nanoparticles was employed and added into photo-curable resin for obtaining the conductive percolation threshold of the polymer composites and measuring the piezoresistivity of the polymer composites. The conductive percolation threshold is around 1.0~1.5wt%, and the gauge factor of 1.5wt% is 9~17. The gauge factor was found which would decrease with the increasing strains. This study can be applied on fabricating the piezoresistive pressure and tactile sensors which can be used to the agricultural, industrial and various industries.