1987년 한국에서 발생한 렙토스피라병의 혈청역학적 조사

이증훈,박영수,이우곤,김석용,정선식,우준희,박성광,박경희,송영욱,김선영,기정일,최두혁,강성귀,김주완,최강원,김우열,최명식,최인학,장우현,윤성열 대한감염학회 1988 감염 Vol.20 No.3

Human leptospirosis was an unfamiliar disease in Korea until 1984 that outbreak of leptospirosis occurred among farmers and soldiers after field works for harvesting rice. During that time, Lee and Jo confirmed the first Korean cases of leptospirosis by serological test, isolation of causative agent and autopy findings. Afterward several outbreaks occurred also during autumn especially after flood in every years and some characterisitcs of leptospirosis in Korea such as clinical manifestations, serotypes and seroepidemiological features has been revealed by many investigators. Because of the major mode of transmission between rodents and human is by direct contact with leptospiral urine of rodents or contaminated soil by the urine, leptospirosis in Korea has been primarily a disease of person in occupations heavily exposed to contaminated soil or infected urine such as farmer, army and etc. Therefore it seems that leptospirosis is one of the main communicable diseases to be controlled urgently in Korea, for an agricultural people account for almost half of total Korean people. For clarifying the seroepidemiological patterns of human leptospirosis in Korea by sex, month region and main reacting serovars of L. interrogans among acute febrile disease occurred in 1987, 1,773 patient's sers with acute febrile episodes were tested by microagglutination test using 19 representative strains of leptospiral serogroup as antigen. All of those sera were collected from 10 collaborative clinics located in Kyunggi, Kangwon, Chungbuk, Chungnam, Chonbuk, Chonnam province and Seoul. The results wee summerized as follows. 1) Among 1,773 sera of patients with acute febrile episodes, 219 (12.4%) were seropositive to L. interrogans, 487(27.5%) to R. tsutsugamushi, 241(13.6%) to R.typhi and 160(90.0%) to Hantaan virus. 2) Among seropositives to L.interrogans, the male outnumbered the female, 65% and 35%. 3) For age distribution, 26.9% of seropositives to L.interrogans were fifties, 19.6% were forties, 9.1% were sixties, 5.9% were thirties and 4.1% were twenties. 4) Eighty three percent of seropositives had occurred between September and October in 1987 with a peak in September. 5) Main leptospiral serovars reactive to patient's sera were Icterohaemorrhagiae(54.3%), Canicola(31.0%), CH-48(13.2%), Tarassovi(0.9%)and Cynopteri(0.5%). 6) For regional distribution, 65.8% of seropositives to L.interrogans were residents from Chonbuk, 12.3% were Chonnam, 7.3% were Chungnam, 5.5% were Kyunggi and 1.4% were Kangwon.

한국 농촌거주 정상인의 Leptospira Interrogans에 대한 항체보유율 현황

최강원,최성배,국윤호,장우현,우준희,박경희,강재승,차창룡,김양수,김석용,윤종구,김익상,이우곤,최명식,송영욱 대한감염학회 1988 감염 Vol.20 No.3

It has been well known that leptospiroisis is a one of the prevailing acute febrile diseases in Korea. In order to find out the incidence of past infection of L. interrogans among normal population, a total of 1,171 sera obtained from rural residents and tested by microscopic agglutination test. All of normal resident's sera were collected during July in 1987, just before epidemic season, at Keochang county(248 sera), at Wanju county(145 sera), at Wonseoung county(255 sera), at Cholwon county(411 sera) and at Kongju county(112 sera). Seropositive rate against L. interrogans among rural residents was 12.0% and ranged from 2.7% in Kongju county to 18.5% in Cholwon county. There was a higher seropositive rate in males and the majority of seropositives occurred in the age group over 30 yesrs. Icterohaemorrhagiae was a most commonly reacting serotype of L. interrogans and followed by CH-48 and Canicola. The sera reacted with Cynopteri, Ballum, Australis, and Pyrogenes were present also.

Effect of nitric oxide-releasing derivative of indomethacin on Prevotella intermedia lipopolysaccharide-induced production of proinflammatory mediators in murine macrophages

Choe, S.H.,Choi, E.Y.,Hyeon, J.Y.,Choi, I.S.,Kim, S.J. Academic Press 2017 Biochemical and biophysical research communication Vol. No.

The purpose of this study was to investigate the influences of NCX 2121, a nitric oxide (NO)-releasing derivative of indomethacin, upon the generation of proinflammatory mediators using murine macrophages activated by lipopolysaccharide (LPS) isolated from Prevotella intermedia, which is one of the pathogens implicated in periodontal diseases. Inducible NO synthase (iNOS)-derived NO, IL-1β and IL-6 as well as their relevant mRNA were significantly attenuated by NCX 2121 in RAW264.7 cells activated by P. intermedia LPS. NCX 2121 was much more effective than the parental compound indomethacin in reducing these proinflammatory mediators. NCX 2121 triggered induction of heme oxygenase-1 (HO-1) in cells exposed to P. intermedia LPS, and its inhibitory influence upon P. intermedia LPS-elicited NO generation was notably blocked by SnPP treatment. NCX 2121 attenuated NF-κB-dependent SEAP release induced by P. intermedia LPS. NCX 2121 did not display inhibitory action towards IκB-α degradation triggered by LPS. Instead, it significantly diminished nuclear translocation as well as DNA-binding action of NF-κB p50 subunit elicited by P. intermedia LPS. Further, NCX 2121 significantly up-regulated SOCS1 mRNA expression in cells challenged with P. intermedia LPS. In summary, NCX 2121 down-regulates P. intermedia LPS-elicited generation of NO, IL-1β and IL-6 in murine macrophages in a mechanism that involves anti-inflammatory HO-1 induction as well as decrement of NF-κB activation, which may be associated with SOCS1 expression. NCX 2121 may have potential benefits as a host immunomodulatory agent for the therapy of periodontal disease.

Phase I Single Dose Elevation and Food Interaction Study of Q-35, A New Oral Quinolone Antimicrobial Agent

Yim, D. S 大韓臨床藥理學會 1995 臨床藥理學會誌 Vol.3 No.1

Melatonin enhances arsenic trioxide-induced cell death via sustained upregulation of Redd1 expression in breast cancer cells

Yun, S.M.,Woo, S.H.,Oh, S.T.,Hong, S.E.,Choe, T.B.,Ye, S.K.,Kim, E.K.,Seong, M.K.,Kim, H.A.,Noh, W.C.,Lee, J.K.,Jin, H.O.,Lee, Y.H.,Park, I.C. North-Holland 2016 Molecular and cellular endocrinology Vol.422 No.-

Melatonin is implicated in various physiological functions, including anticancer activity. However, the mechanism(s) of its anticancer activity is not well understood. In the present study, we investigated the combined effects of melatonin and arsenic trioxide (ATO) on cell death in human breast cancer cells. Melatonin enhanced the ATO-induced apoptotic cell death via changes in the protein levels of Survivin, Bcl-2, and Bax, thus affecting cytochrome c release from the mitochondria to the cytosol. Interestingly, we found that the cell death induced by co-treatment with melatonin and ATO was mediated by sustained upregulation of Redd1, which was associated with increased production of reactive oxygen species (ROS). Combined treatment with melatonin and ATO induced the phosphorylation of JNK and p38 MAP kinase downstream from Redd1 expression. Rapamycin and S6K1 siRNA enhanced, while activation of mTORC1 by transfection with TSC2 siRNA suppressed the cell death induced by melatonin and ATO treatment. Taken together, our findings suggest that melatonin enhances ATO-induced apoptotic cell death via sustained upregulation of Redd1 expression and inhibition of mTORC1 upstream of the activation of the p38/JNK pathways in human breast cancer cells.

Population Pharmacokinetic Study of Aminoglycoside Antibiotics in Korean Population

Yim, D. S 大韓臨床藥理學會 1995 臨床藥理學會誌 Vol.3 No.1

젖소의 초음파 유도 채란율에 대한 FSH 전처리 효과의 비교

이정규,최상용,박충생,이효종,조성근,공일근,박성재,강태영,최민철,최창용,손우진 경상대학교 축산진흥연구소 1997 畜産振興硏究所報 Vol.24 No.1

NCX 4040, a nitric oxide-donating aspirin derivative, inhibits Prevotella intermedia lipopolysaccharide-induced production of proinflammatory mediators in murine macrophages

Choi, E.Y.,Choe, S.H.,Hyeon, J.Y.,Park, H.R.,Choi, J.I.,Choi, I.S.,Kim, S.J. North-Holland ; Elsevier Science Ltd 2015 european journal of pharmacology Vol.768 No.-

In this study, the effects and underlying mechanisms of NCX 4040, a nitric oxide (NO)-donating aspirin derivative, on the production of proinflammatory mediators were examined using murine macrophages exposed to lipopolysaccharide (LPS) from Prevotella intermedia, a pathogen implicated in the etiology of periodontal disease. NCX 4040 significantly reduced P. intermedia LPS-induced production of inducible NO synthase (iNOS)-derived NO, IL-1β and IL-6 as well as their mRNA expression in RAW264.7 cells. Notably, NCX 4040 was much more effective than the parental compound aspirin in reducing LPS-induced production of inflammatory mediators. NCX 4040 induced the expression of heme oxygenase-1 (HO-1) in cells treated with P. intermedia LPS, and the suppressive effect of NCX 4040 on LPS-induced NO production was significantly reversed by SnPP, a competitive HO-1 inhibitor. NCX 4040 did not influence LPS-induced phosphorylation of JNK and p38. IκB-α degradation as well as nuclear translocation and DNA-binding activities of NF-κB p65 and p50 subunits induced by P. intermedia LPS were significantly reduced by NCX 4040. Besides, LPS-induced phosphorylation of STAT1 and STAT3 was significantly down-regulated by NCX 4040. Further, NCX 4040 elevated the SOCS1 mRNA in cells stimulated with LPS. This study indicates that NCX 4040 inhibits P. intermedia LPS-induced production of NO, IL-1β and IL-6 in murine macrophages through anti-inflammatory HO-1 induction and suppression of NF-κB, STAT1 and STAT3 activation, which is associated with the activation of SOCS1 signaling. NCX 4040 could potentially be a promising tool in the treatment of periodontal disease, although further studies are required to verify this.

수소취성의 무서운 공포

최병학(B.G Choe),민두식(D.S Min),심윤임(Y.I Shim),최송천(S.C Choi),이장우(J.W Lee),김우식(W.S Kim) 대한기계학회 2010 대한기계학회 춘추학술대회 Vol.2010 No.11

1. 고온, Hydrogen Attack, 펄라이트 분해(메탄반응) 2. 저온, Hydrogen Embrittlement, Delayed 균열 3. 응력/부식, Hydrogen Stress Corrosion, S/CI 집적동반 수소취성을 대하며 첫 번째 결정은 이것을 손상분류체계 중 어디에 속하게 할 것인가 이었는데, 비교적 큰 갈등 없이 사용상>피로/부식/고온 분류 중 부식에 넣는다. 고전적인 부식은 틈새/갈바닉/응력-부식/입계예민화/피트/마식/염기성부식의 7개 분야로 나눌 수 있는데, 여기에 특별히 “수소취성” 부식을 여덟 번째로 더하는 것이다. 아주 잦지는 않지만 그래도 종종 손상진단 사건들에서 수소취성 관련 의심 증거들이 나타난다. 그런데 사실 손상진단 현장에서 자신 있게 수소취성을 원인으로 내세우는 것은 실험적인 증거가 미약하여 쉽지 않다. 이것은 원자번호 1번인 작은 수소(분자 또는 이온형태)가 금속과 어떤 작용을 하는 가에 대한 분석이 어렵기 때문이다. 본 연구는 수소취성을 종합 정리해 보고자한 것이다. 우선 온도에 따른 반응기구별 분류를 하였는데, 잘 밝혀보지 않은 것은 부식관련 기구로 마지막 분류체계에 넣었다. 이와 같은 분류체계가 수소취성의 “The best”일지는 확신할 수 없다. 하지만 현장에서 종종 의심되는 수소취성의 손상사건들을 인단 정형화된 분류 틀에 넣어 진단 논리를 단순화할 필요가 크다.

Agomelatine, a MT₁/MT₂ melatonergic receptor agonist with serotonin 5-HT_2C receptor antagonistic properties, suppresses Prevotella intermedia lipopolysaccharide-induced production of proinflammatory mediators in murine ma

Hyeon, J.Y.,Choi, E.Y.,Choe, S.H.,Park, H.R.,Choi, J.I.,Choi, I.S.,Kim, S.J. Pergamon Press 2017 Archives of oral biology Vol.82 No.-

Objective: This study was performed in an attempt to examine the influence of agomelatine in mitigating the generation of proinflammatory mediators in RAW264.7 murine macrophages exposed to lipopolysaccharide (LPS) obtained from Prevotella intermedia, a gram-negative anaerobic bacterium that is related with various types of periodontal diseases, and the molecular mechanisms behind its effects. Design: LPS from P. intermedia strain ATCC 25611 was prepared employing the conventional phenol-water procedure. Conditioned culture media were analyzed for the levels of nitric oxide (NO), interleukin-1β (IL-1β) and IL-6. Real-time PCR analysis was carried out to determine the mRNA levels of inducible NO synthase (iNOS), IL-1β, IL-6 and SOCS1. Protein expression levels were evaluated by immunoblot test. NF-κB-dependent SEAP reporter assay was performed using a reporter cell line. DNA-binding activities of NF-κB subunits were analyzed utilizing the ELISA-based kits. Results: Agomelatine was found to down-regulate significantly the generation of iNOS-derived NO, IL-1β and IL-6 as well as the expression of their mRNAs in cells activated with P. intermedia LPS. Agomelatine decreased NF-κB-dependent SEAP release caused by P. intermedia LPS. Agomelatine did not inhibit NF-κB transcription induced by LPS at the level of IκB-α degradation. Instead, LPS-induced nuclear translocation and DNA binding of NF-κB p50 subunit was blocked by agomelatine. P. intermedia LPS-elicited activation of STAT1 and STAT3 was reduced notably by co-treatment with agomelatine. Agomelatine showed a tendency to enhance mRNA level of SOCS1 in LPS-activated cells as well. Conclusions: Agomelatine merits further evaluation to reveal its usefulness on the host modulation of periodontal disease.