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      • KCI등재

        신원 은닉을 위한 두뇌 영상의 무손실 변경

        이효종,Lee, Hyo-Jong,Yu, Du Ruo 한국정보처리학회 2011 정보처리학회논문지B Vol.18 No.6

        디지털 형태로 저장된 의료정보가 네트워크를 통하여 제약 없이 전송될 수 있게 되면서, 환자의 개인정보 관리는 의료 업계에서 중요한 주제로 부각되었다. 현재 두뇌 영상의 의료정보를 보호하는 방법은 환자의 신원을 은닉시키기 위하여 얼굴을 절삭하는 것이다. 그러나 절삭 과정에서 간혹 중요한 두뇌 조직부가 함께 절단되어 탈면 두뇌 영상은 의료 용도로 활용될 수 없게 손상을 입게 된다. 실린더 모양의 마스크를 덧붙임으로써 두뇌 영상의 중요한 모든 정보를 유지하면서 환자의 신원 정보를 은닉시키는 직접적인 방법을 제안하였다. 제안하는 두뇌 영상의 무손실 변경 방법은 중요한 영상정보가 손상되지 않음을 확인하였다. 또한 마스크로 입혀진 두뇌영상의 신원을 확인할 수 없는 사실도 증명되었다. Patients' privacy protection is a heated issue in medical business, as medical information in digital format transmit everywhere through networks without any limitation. A current protection method for brain images is to deface from the brain image for patient's privacy. However, the defacing process often removes important brain voxels so that the defaced brain image is damaged for medical analysis. An ad-hoc method is proposed to conceal patient's identification by adding cylindrical mask, while the brain keep all important brain voxels. The proposed lossless deformation of brain image is verified not to loose any important voxels. Futhermore, the masked brain image is proved not to be recognized by others.

      • SCOPUSKCI등재

        반복핵이식에 의한 복제동물 생산에 관한 연구 III. 토끼에서 제3세대 복제수정란의 생산

        이효종,전병균,윤희준,박충생,최상용,윤창현,강대진,Lee Hyo-jong,Jeon Byeong-gyun,Yin Xi-jun,Park Choong-saeng,Choe Sang-yong,Yun Chang-hyun,Kang Dae-jin 한국임상수의학회 1995 한국임상수의학회지 Vol.12 No.1

        The recycling nuclear transplantation(NT) technique has the powerful potential of producing a large number of genetically identical embryos and offsprings from one embryo. Multiple generational cloning by this technique utilizes the NT embryo itself as the donor for the next generation of cloning. In this experiment, we have produced the third generational cloned embryos by recycling NT. Further we examined comparatively the electrofusion rate and in vitro developmental potential in the cloned embryos of the first second and third generations. The embryos of 16-cell stage were collected from the mated does by flushing oviducts with Dulberco's phosphate buffered saline containing 10 % fetal calf serum(FCS) at 47 hours after hCG injection. In the first generation NT, the nuclear donor embryos were synchronized in the phase of Gl/S transition of 32-cell stage. The first and second generation NT embryos developed to 16-cell were used as donor nuclei for second and third generation. The recipient cytoplasms were utilized the oocytes collected at 14 hours after hCG injection, following revoming the nucleus and the first polar body by micromanipulation. The separated blastomeres were injected into the enucleated recipient oocytes by micromanipulation and were fused by electrical stimulation. The electrofusion rate was seen to be 78.0, 88.0 and 90.3 % in the first second and third generation NT rabbit embryos, respectively. The fused oocytes were co-cultured with a monolayer of rabbit oviductal epithelial cells in M-199 solution containing 10 % FCS for 120 hours at 39$^{\circ}C$ in a 5% $CO_2$ incubator. The in vitro developmental potential to blastocyst stage was significantly(P<0.05) decreased in the third(7.2 %) generation NT embryos compared to the first(53.1 %) and second(16.1 %) generation NT embryos. Following in vitro development to blastocyst stage, they were stained with Hoechst 33342 dye for counting the number of blastomeres by fluorescence microscopy. The mean blastomere numbers and cell cycle numbers of NT embryos during the culture period were significantly(p<0.05) decreased in the second(93.9 cells and 6.55 cylces) and third(81.5 cells and 1.35 cylces) generation, compared to the first(189.9 cells and 7.55 cylces) generation.

      • KCI등재

        과배란처리 한우에서 초음파 유도에 의한 난자채란에 대한 연구

        이효종,강태영,조성근,박준규,손우진,최민철,최상용 韓國受精卵移植學會 1997 한국동물생명공학회지 Vol.12 No.2

        The present study was carried out to evaluate the effect of superovulation treatments on ovarian responses, oocyte recovery rates and grades of collected oocytes using an ultrasound-guided transvaginal approach in Korean native cows. Superovulation in cows was induced with two different regimenes: 1) FSH-decreasing dose(n=8): the cows were received twice per day for three days of the total dose of 400 mg of FSH-p, 2) FSH-single dose(n=9): the cows were administrated a single dose of 400 mg of FSH-p in 25% PVP. The Observation of visible follicles and collection of oocytes were performed 12 hours following the last FSH in FSH-decreasing dose group and 48 hours after the FSH-single dose injection. All visible follicles larger than 6 mm were punctured and aspirated with a 6.5 MHz convex-array ultrasound transducer designed for intravaginal use. The mean number of visible follicles(> 6 mm) was significantly(P<0.05) higher in the FSH-decreasing dose treatment (22.811.9) and FSH-single dose treatment (20.612.0) groups than the non-treatment group(7.08). The mean recovery rate of oocytes was not significantly(P<0.05) different between the treatment and control groups, but the mean number of collected oocytes was significantly(P<0.05) higher in the FSH-decreasing dose treatment( 12.611.5) and FSH-single dose treatment (11.813.6) groups than the non-treatment group(3.70.5). In conclusion, the FSH-single dose treatment at superovulation in cows for ultrasound-guided aspiration might increase the number of aspiratable follicles and the recovery rate of follicular oocytes as the FSH-decreasing dose treatment.

      • KCI등재후보
      • KCI등재

        소 수정란에서 Green Fluorescent Protein 유전자 검색 및 PCR에 의한 성감별

        이효종,강태영,노규진,채영진,이항,최상용 韓國受精卵移植學會 2000 한국동물생명공학회지 Vol.15 No.2

        The efficiency of transgenic livestock production could be improved by early screening of transgene-integration and sexing of embryos at preimplantational stages before trasferring them into recipients. We examined the effciency of multiplex PCR analysis for the simultaneous confirmation of the trasgene and sex during the preimplantational development of bovine embryos and the possibility of green fluorescent protein(GFP) gene as a non-invasive marker for the early screening of transgenic embryos. The GFP gene was microinjected into the male pronuclei of bovine zygotes produced in vitro. The injected zygotes were co-cultured in TCM-199 containing 10% FCS with boving oviductal epithelial cells in a 5% CO2 incubator. Seventeen(13.0%) out of 136 gene-injected bovine zygotes developed by multiplex PCR analysis and the expression of GFP was detected by observing green fluorescence in embryos under a fluorescent microscope. Eight(67%) of 12 embryos at 2-cell to blastocyst stage were positive in the PCR analysis, but only two(11.8%) of 17 blastocysts expressed the GFP gene. Their sex was determined as 7 female and 5 male embryos by the PCR analysis. The results indicate that the screening of GFP gene and sex in bovine embryos by PCR analysis and fluorescence detection could be a promisible method for the preselection of transgenic embryos.

      • KCI등재후보
      • 객체지향 프로그래밍 기법에 의한 원격학습도구의 개발

        이효종,Lee, Hyo-Jong 한국정보처리학회 2000 정보처리논문지 Vol.7 No.11

        세계적으로 급속히 발전하고 있는 웹(WWW) 기술은 원격학습의 새로운 기회를 인터넷을 통하여 제공하고 있다. 가상 교육 또는 원격 교육 운영에 관한 일부 실험에 관한 성공적인 보고도 나와있다. 웹은 다른 네트워크 도구와 병합하여 원격으로 떨어진 학습자들에게 대화형 학습을 할 수 있는 가상 교실을 생성할 수 있음을 연구하였다. 전자우편, 다자참여 학습보드, 뉴스그룹, 또는 화상회의 같은 원격 학습 도구의 표준에 관한 필요 사항들을 연구하고, 자바 프로그래밍 언어와 객체지향 프로그래밍 기법을 이용하여 이들 학습 도구들을 개발하였다. 객체지향 프로그래밍을 통하여 개발되는 코드의 내구성, 확장성 및 재활용성을 증가시킬 수 있었다. 이들 도구를 활용하여 개발된 시스템은 교사와 학생, 또는 학생들 간에 상호작용을 허용하여 실질적인 원격 교육 시스템에 사용 될 수 있음을 알 수 있었다. The rapidly developing World Wide Web technology provides new opportunities for distance education over the internet. Several successful experiments about cyber educationor distance leanning have been reported. The Web when combined with other network tools can be used to create a virtual classroom to bring together a community of learners for interactive education. Requrements for standard tools for distance. Iearning, such as an electionic mail, a multiparheipant bcard newsgroup service and video conference tools have been investigated and implemented based on the object modeling technique useing java programming language. The object onented programming helps the developed codes maintain learning with allowed interactions either between instroctors and students or between students.

      • KCI등재

        질감을 이용한 차량모델 인식 알고리즘

        이효종 한국정보처리학회 2005 정보처리학회논문지. 소프트웨어 및 데이터 공학 Vol.12 No.3

        사회가 발전하면서 자동차의 수요도 세계적으로 급증하고 있다. 교통제어나 차량에 연관된 범죄 등을 해결하는데 자동차의 인식 기술이 중요하기 때문에 이에 관련된 번호판 인식이나 교통량 측정에 관한 연구는 오래 전부터 수행되어왔다. 본 논문에서는 주행차량의 제조회사와 차량 모델을 인식하는 방법을 제시하였다. 차종의 인식은 차량 전면부 영역의 질감을 이용하여 인식하였다. 번호판 상단의 라디에이터 영역에서 질감 특징자를 추출하여 신경망을 통한 차종별 학습을 시켜서 인식을 시도하였다. 제안 알고리즘에서 차종의 정인식은 93.7%, 이종차량의 감별은 99.7%로 양호하게 나타났다. The number of vehicles are rapidly increased as our society is developed. The vehicle recognition has been studied for a while because many people acknowledged it has critical functions to solve the problems of traffic control or vehicle-related crimes. In this paper a novel method is proposed to recognize vehicle models corresponding makers. Vehicles' models are recognized based on the texture parameters from segmented radiator region above a number plate. A three-layer neural network was built and trained with the texture features for recognition. The proposed method shows 93.7% of recognition rate and 99.7% of specificity for vehicles' model.

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