Visualizing the distributions and spatiotemporal changes of metabolites in Panax notoginseng by MALDI mass spectrometry imaging

Chenglong Sun,Shuangshuang Ma,Lili Li,Daijie Wang,Wei Liu,Feng Liu,Lanping Guo,Xiao Wang 고려인삼학회 2021 Journal of Ginseng Research Vol.45 No.6

Background: Panax notoginseng is a highly valued medicinal herb used widely in China and many Asiancountries. Its root and rhizome have long been used for the treatment of cardiovascular and hematologicaldiseases. Imaging the spatial distributions and dynamics of metabolites in heterogeneous planttissues is significant for characterizing the metabolic networks of Panax notoginseng, and this will alsoprovide a highly informative approach to understand the complex molecular changes in the processing ofPanax notoginseng. Methods: Here, a high-sensitive MALDI-MS imaging method was developed and adopted to visualize thespatial distributions and spatiotemporal changes of metabolites in different botanical parts of Panaxnotoginseng. Results: A wide spectrum of metabolites including notoginsenosides, ginsenosides, amino acids, dencichine,gluconic acid, and low-molecular-weight organic acids were imaged in Panax notoginseng rhizomeand root tissues for the first time. Moreover, the spatiotemporal alterations of metabolites during thesteaming of Panax notoginseng root were also characterized in this study. And, a series of metabolitessuch as dencichine, arginine and glutamine that changed with the steaming of Panax notoginseng weresuccessfully screened out and imaged. Conclusion: These spatially-resolved metabolite data not only enhance our understanding of the Panaxnotoginseng metabolic networks, but also provide dire

Clusterin negatively modulates mechanical stress-mediated ligamentum flavum hypertrophy through TGF-β1 signaling

Liu Chunlei,Li Peng,Ao Xiang,Lian Zhengnan,Liu Jie,Li Chenglong,Huang Minjun,Wang Liang,Zhang Zhongmin 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

Ligamentum flavum hypertrophy (LFH) is a major cause of lumbar spinal canal stenosis (LSCS). The pathomechanisms for LFH have not been fully elucidated. Isobaric tags for relative and absolute quantitation (iTRAQ) technology, proteomics assessments of human ligamentum flavum (LF), and successive assays were performed to explore the effect of clusterin (CLU) upregulation on LFH pathogenesis. LFH samples exhibited higher cell positive rates of the CLU, TGF-β1, α-SMA, ALK5 and p-SMAD3 proteins than non-LFH samples. Mechanical stress and TGF-β1 initiated CLU expression in LF cells. Notably, CLU inhibited the expression of mechanical stress-stimulated and TGF-β1-stimulated COL1A2 and α-SMA. Mechanistic studies showed that CLU inhibited mechanical stress-stimulated and TGF-β1-induced SMAD3 activities through suppression of the phosphorylation of SMAD3 and by inhibiting its nuclear translocation by competitively binding to ALK5. PRKD3 stabilized CLU protein by inhibiting lysosomal distribution and degradation of CLU. CLU attenuated mechanical stress-induced LFH in vivo. In summary, the findings showed that CLU attenuates mechanical stress-induced LFH by modulating the TGF-β1 pathways in vitro and in vivo. These findings imply that CLU is induced by mechanical stress and TGF-β1 and inhibits LF fibrotic responses via negative feedback regulation of the TGF-β1 pathway. These findings indicate that CLU is a potential treatment target for LFH.

Ampelopsin alleviates sevoflurane-induced cognitive dysfunction by mediating NF-κB pathway in aged rats

Chenglong Liu,Xiaojuan Zha,Haihua Liu,Fang Wei,Fei Zhang 한국유전학회 2020 Genes & Genomics Vol.42 No.4

Background Cancer-induced bone pain (CIBP) is the pain caused by bone metastasis from malignant tumors, and the largest source of pain for cancer patients. miR-300 is an important miRNA in cancer. It has been shown that miR-300 regulates tumorigenesis of various tumors. Purpose This study aims to investigate the role of miR-300 in CIBP and its underlying molecular mechanisms in vitro and in vivo. Methods We constructed CIBP model in rats and investigated the mechanism through which miR-300 affects CIBP. We first examined expression level of miR-300 in CIBP rats and then tested the effect of its overexpression. Next, we identified the target of miR-300 using TargetScan analysis and double luciferase assay. Finally, we studied genetic interactions between miR-300 and its target and their roles in CIBP. Results We found that miR-300 was downregulated in CIBP rats. Overexpression of miR-300 significantly attenuated cancerinduced neuropathic pain (p < 0.01). Furthermore, TargetScan analysis and double luciferase assay show High Mobility Group Box 1 (HMGB1) is a target of miR-300. Notably, HMGB1 is overexpressed in CIBP rats, while up-regulation of miR300 significantly suppresses expression of HMGB1 (p < 0.01). Moreover, knockdown of HMGB1 by siRNA significantly relieves cancer-induced neuropathic pain in rats (p < 0.01). On the other hand, HMGB1 overexpression partially blocked the effect of miR-300 on cancer-induced nerve pain. Conclusion miR-300 relieves cancer-induced neuropathic pain by inhibiting HMGB1 expression. These results may be beneficial for the treatment of CIBP in clinical practice.

Thermal-hydraulic research on rod bundle in the LBE fast reactor with grid spacer

Liu Jie,Song Ping,Zhang Dalin,Wang Shibao,Lin Chao,Liu Yapeng,Zhou Lei,Wang Chenglong,Tian Wenxi,Qiu Suizheng,Su G.H. 한국원자력학회 2022 Nuclear Engineering and Technology Vol.54 No.7

The research on the flow and heat transfer characteristics of lead bismuth(LBE) is significant for the thermal-hydraulic calculation, safety analysis and practical application of lead-based fast reactors(LFR). In this paper, a new CFD model is proposed to solve the thermal-hydraulic analysis of LBE. The model includes two parts: turbulent model and turbulent Prandtl, which are the important factors for LBE. In order to find the best model, the experiment data and design of 19-pin hexagonal rod bundle with spacer grid, undertaken at the Karlsruhe Liquid Metal Laboratory (KALLA) are used for CFD calculation. Furthermore, the turbulent model includes SST k u and k ε; the turbulent Prandtl includes Cheng-Tak and constant (Prt ¼1.5,2.0,2.5,3.0). Among them, the combination between SST k u and Cheng-Tak is more suitable for the experiment. But in the low Pe region, the deviation between the experiment data and CFD result is too much. The reason may be the inlet-effect and when Pe is in a low level, the number of molecular thermal diffusion occupies an absolute advantage, and the buoyancy will enhance. In order to test and verify versatility of the model, the NCCL performed by the Nuclear Thermal-hydraulic Laboratory (Nuthel) of Xi'an Jiao tong University is used for CFD to calculate. This paper provides two verification examples for the new universal model

miR-300 mitigates cancer-induced bone pain through targeting HMGB1 in rat models

Chenglong Liu,Jianping Yang,Haihua Liu,Tian Xia,Fei Zhang 한국유전학회 2020 Genes & Genomics Vol.42 No.3

Background Cancer-induced bone pain (CIBP) is the pain caused by bone metastasis from malignant tumors, and the largest source of pain for cancer patients. miR-300 is an important miRNA in cancer. It has been shown that miR-300 regulates tumorigenesis of various tumors. Objective This study aims to investigate the role of miR-300 in CIBP and its underlying molecular mechanisms in vitro and in vivo. Methods We constructed CIBP model in rats and investigated the mechanism through which miR-300 affects CIBP. We first examined expression level of miR-300 in CIBP rats and then tested the effect of its overexpression. Next, we identified the target of miR-300 using TargetScan analysis and double luciferase assay. Finally, we studied genetic interactions between miR-300 and its target and their roles in CIBP. Results We found that miR-300 was downregulated in CIBP rats. Overexpression of miR-300 significantly attenuated cancerinduced neuropathic pain (p < 0.01). Furthermore, TargetScan analysis and double luciferase assay show High Mobility Group Box 1 (HMGB1) is a target of miR-300. Notably, HMGB1 is overexpressed in CIBP rats, while up-regulation of miR-300 significantly suppresses expression of HMGB1 (p < 0.01). Moreover, knockdown of HMGB1 by siRNA significantly relieves cancer-induced neuropathic pain in rats (p < 0.01). On the other hand, HMGB1 overexpression partially blocked the effect of miR-300 on cancer-induced nerve pain. Conclusion miR-300 relieves cancer-induced neuropathic pain by inhibiting HMGB1 expression. These results may be beneficial for the treatment of CIBP in clinical practice.

Detrital-zircon geochronology and Hf isotope of Paleozoic sedimentary rocks in the Jilin Province, NE China: tectonic significance for microcontinental blocks of eastern Central Asian Orogenic Belt

Chenglong Shi,Xiaozhong Ding,Yanxue Liu,Xiaodong Zhou 한국지질과학협의회 2019 Geosciences Journal Vol.23 No.5

We undertook U-Pb-Hf isotope testing of detrital zircons from Late Paleozoic sedimentary rocks in the Jilin Province, NE China, with the aim of constraining the tectonic evolution of eastern Central Asian Orogenic Belt. The age populations of the detrital zircons from the sandstone of the Jiefangcun Formation in the Songliao Block are ~2234, ~1782, ~1030–921, ~817, ~540, and ~381–254 Ma, whereas those of the zircons from the greywacke of the Permian Kedao Formation in the Songliao Block yield are ~2436, ~1905, ~1692, 1146–1170, ~554–498, and ~342–255 Ma. Combined Hf isotope characteristics, these ages reveal that: (1) rocks from the Jiefangcun and Kedao formations were deposited after 254 and 255 Ma, respectively; (2) these formations were sourced mainly from the Songliao Block and the Jiamus Terrane in the eastern CAOB and to a lesser amount, from the northern margin of the NCC; and (3) it appears that several remnants of ancient Precambrian basement occur adjacent to these Late Paleozoic strata that occur within the microcontinental blocks. Detrital zircons from the silty sandstone of the Miaoling Formation in the western margin of the Khanka Terrane yield peak age populations of 508–476, 463–396, and 348–258 Ma, having similar εHf(t) values to those obtained from zircons within the Jiamusi Terrane. These data indicate that (1) deposition of the Miaoling Formation in the western margin of the Khanka Terrane had started by 258 Ma, and (2) these sediments were sourced mainly from the Jiamusi Terrane and local magmatic rocks. The widespread occurrence of detrital zircons with ages of 480–550 Ma and positive εHf(t) values within the Late Paleozoic strata in the Songliao Block and the Jiamusi–Khanka Terrane, indicates that the amalgamation of these blocks took place during the middle stage of the Paleozoic, probably after the Silurian, prior to the Late Permian.

Neural Network-based Event-triggered Adaptive Asymptotic Tracking Control for Switched Nonlinear Systems

Chenglong Zhu,Baomin Li,Jianwei Xia,Na Zhang,Rui Liu 제어·로봇·시스템학회 2022 International Journal of Control, Automation, and Vol.20 No.6

In this paper, an adaptive event-triggered asymptotic tracking control problem is addressed for switched nonlinear systems with unknown control directions. In existing control schemes, the proposed controller is not directly aimed at the original system, which affects the control performance. Different from the existing control schemes, based on the original system, an event-triggered control law is constructed in this paper. The proposed event-triggered controller guarantees that the tracking error ς1 asymptotically converges to the origin. Finally, the effectiveness of the proposed controller design scheme is proved by simulation examples.

Expediting the Formation of Cellulose Nanopaper by Heterocoagulation of Oppositely Charged Cellulose Nanofibers

Meng Kang,Chenglong Li,Dehai Yu,Guodong Li,Zhaoping Song,Huili Wang,Wenxia Liu 한국섬유공학회 2022 Fibers and polymers Vol.23 No.7

Cellulose nanopaper has attracted tremendous attention as an alternative to fossil-based flexible substrates. However, the fabrication of nanopaper from cellulose nanofibers (CNFs) is a time-consuming process. Herein, positivelycharged CNFs (PCNFs) were employed to heterocoagulate negatively charged CNFs (NCNFs) at various PCNF-to-NCNFratios in order to decrease the formation time of nanopaper. At a PCNF-to-NCNF ratio of 1:1, the formation time was reducedfrom 98 min to 20 min. Although heterocoagulation increases the roughness of cellulose nanopaper, nanopapers made from amixture of NCNFs and PCNFs still possess a nanosized network structure and therefore show similar transparency tonanopapers made of pure NCNFs. Compared to the nanopaper made from pure NCNFs, the nanopaper made from themixture of NCNFs and PCNFs showed reduced tensile strength but improved resistance to deformation. This study providesa practical method for the production of cellulose nanopaper.

EIS diagnosis on the corrosion behavior of TiN coated NiTi surgical alloy

Dazhi Yang,Chenglong Liu,Xiaopeng Liu,Min Qi,Guoqiang Lin 한국물리학회 2005 Current Applied Physics Vol.5 No.5

In this work titanium nitride (TiN) coatings were deposited on NiTi surgical alloy by arc ion plating (AIP). The open circuittesting and electrochemical impedance spectroscopy (EIS) have been employed to study the corrosion performance of the TiNcoated samples in Troyde.s simulated body uid. It was found that the TiN coating showed a good corrosion resistance at the begin-ning of long-term immersion; with the increase of immersion time, however, the corrosion resistance of the coating deteriorated afternearly 24 h of immersion; moreover, the large areas of the coating had fallen o the substrate after 30 days of immersion..

Comfort-Based Trajectory and Velocity Planning for Automated Vehicles Considering Road Conditions

Yan Shengyu,Liu Chenglong,Cao Jing 한국자동차공학회 2021 International journal of automotive technology Vol.22 No.4

Driving comfort significantly affects travel experiences. However, the most prevailing automated vehicle (AV) control strategies mainly focus on the safety and efficiency concerns, neglecting the comfort perspective. Moreover, pavement conditions, such as roughness and distress, cannot be directly detected by standard AV onboard sensors, although they are essential factors impacting driving comfort. This study aimed to present a vehicle-to-infrastructure system to adjust AV trajectories for alleviating the negative impacts of bumpy roads on comfort. The precollected lane-based roadway roughness information was used to optimally adjust the longitudinal and lateral AV trajectories. The method was validated by an eight-degree-of-freedom full-car simulation model. Specifically, the relationships among comfort, jerk, speed, and pavement parameters were revealed and applied to the adjustment process. The results showed that the trajectory adjustment approach improved the overall comfort level by 30 % in the numerical case. Furthermore, a framework was designed to facilitate data exchanges between vehicles and infrastructures in empirical scenarios. The proposed method had the potential to assist AVs in better-perceived driving quality.