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Mu Danni,Fang Jiadan,Yu Songlin,Ma Yichen,Cheng Jin,Hu Yingying,Song Ailing,Zhao Fang,Zhang Qi,Qi Zhihong,Zhang Kui,Xia Liangyu,Qiu Ling,Zhu Huijuan,Cheng Xinqi 대한진단검사의학회 2024 Annals of Laboratory Medicine Vol.44 No.1
Background: Twenty-four-hour urinary free cortisol (UFC) measurement is the initial diagnostic test for Cushing’s syndrome (CS). We compared UFC determination by both direct and extraction immunoassays using Abbott Architect, Siemens Atellica Solution, and Beckman DxI800 with liquid chromatography-tandem mass spectrometry (LC-MS/MS). In addition, we evaluated the value of 24-hr UFC measured by six methods for diagnosing CS. Methods: Residual 24-hr urine samples of 94 CS and 246 non-CS patients were collected. A laboratory-developed LC-MS/MS method was used as reference. UFC was measured by direct assays (D) using Abbott, Siemens, and Beckman platforms and by extraction assays (E) using Siemens and Beckman platforms. Method was compared using Passing–Bablok regression and Bland–Altman plot analyses. Cut-off values for the six assays and corresponding sensitivities and specificities were calculated by ROC analysis. Results: Abbott-D, Beckman-E, Siemens-E, and Siemens-D showed strong correlations with LC-MS/MS (Spearman coefficient r=0.965, 0.922, 0.922, and 0.897, respectively), while Beckman-D showed weaker correlation (r=0.755). All immunoassays showed proportionally positive bias. The areas under the curve were 0.975 for Abbott-D, 0.972 for LC-MS/MS, 0.966 for Siemens-E, 0.948 for Siemens-D, 0.955 for Beckman-E, and 0.877 for Beckman-D. The cut-off values varied significantly (154.8–1,321.5 nmol/24 hrs). Assay sensitivity and specificity ranged from 76.1% to 93.2% and from 93.0% to 97.1%, respectively. Conclusions: Commercially available immunoassays for measuring UFC show different levels of analytical consistency compared to LC-MS/MS. Abbott-D, Siemens-E, and Beckman-E have high diagnostic accuracy for CS.
Zhang Xiaoyang,Xiong Si-Yi,Wu Xiukun,Zeng Bei-Bei,Mo Yang-Mei,Deng Zhi-Cheng,Wei Qi,Gao Yang,Cui Licao,Liu Jianping,Long Haozhi 한국미생물학회 2023 The journal of microbiology Vol.61 No.11
Establishing slash pine plantations is the primary method for restoring sandification land in the Houtian area of South China. However, the microbial variation pattern with increasing stand age remains unclear. In this study, we investigated microbial community structure and function in bare sandy land and four stand age gradients, exploring ecological processes that determine their assembly. We did not observe a significant increase in the absolute abundance of bacteria or fungi with stand age. Bacterial communities were dominated by Chloroflexi, Actinobacteria, Proteobacteria, and Acidobacteria; the relative abundance of Chloroflexi significantly declined while Proteobacteria and Acidobacteria significantly increased with stand age. Fungal communities showed succession at the genus level, with Pisolithus most abundant in soils of younger stands (1- and 6-year-old). Turnover of fungal communities was primarily driven by stochastic processes; both deterministic and stochastic processes influenced the assembly of bacterial communities, with the relative importance of stochastic processes gradually increasing with stand age. Bacterial and fungal communities showed the strongest correlation with the diameter at breast height, followed by soil available phosphorus and water content. Notably, there was a significant increase in the relative abundance of functional groups involved in nitrogen fixation and uptake as stand age increased. Overall, this study highlights the important effects of slash pine stand age on microbial communities in sandy lands and suggests attention to the nitrogen and phosphorus requirements of slash pine plantations in the later stages of sandy management.
Epidemiological Characteristics of Norovirus Outbreaks in Shenyang from 2017 to 2021
Qi Ying,Dong Xinxin,Cheng Xiaowei,Xu Han,Wang Jin,Wang Bing,Chen Ye,Sun Baijun,Zhang Linlin,Yao Yan 한국미생물학회 2023 The journal of microbiology Vol.61 No.4
Norovirus is one of the leading causes of acute gastroenteritis outbreaks worldwide. This study aimed to identify the epidemiological characteristics of norovirus outbreaks and to provide evidence for public health entities. Specimens and epidemiological survey data were collected to determine if there were differences in the attack rate of norovirus in terms of the year, season, transmission route, exposure setting, and region and to determine whether there were relationships between the reporting interval, the number of illnesses in a single outbreak and the duration of the outbreak. Norovirus outbreaks were reported throughout the year, with seasonal characteristics (i.e., high rates in spring and winter). Among all regions in Shenyang with the exception of Huanggu and Liaozhong, norovirus outbreaks had been reported, and the primary genotype was GII.2[P16]. Vomiting was the most common symptom. The main places of occurrence were childcare institutions and schools. The person-to-person route was the main transmission route. The median duration of norovirus was 3 days (IQR [interquartile range]: 2–6 days), the median reporting interval was 2 days (IQR: 1–4 days), the median number of illnesses in a single outbreak was 16 (IQR: 10–25); there was a positive correlation between these parameters. Norovirus surveillance and genotyping studies still need to be further strengthened to increase knowledge regarding the pathogens and their variant characteristics, to better characterize the patterns of norovirus outbreaks and to provide information for outbreak prevention. Norovirus outbreaks should be detected, reported and handled early. Public health entities and the government should develop corresponding measures for different seasons, transmission routes, exposure settings, and regions.
A new species of Stibadocerella Brunetti, 1918 from China (Diptera, Cylindrotomidae)
Zhang Bing,Gao Shang,Yang Qi-Cheng,Ren Jin-Long,Yang Ding 한국응용곤충학회 2022 Journal of Asia-Pacific Entomology Vol.25 No.3
Four species of the genus Stibadocerella Brunetti, 1918 were previously known from the world. Here one new species, Stibadocerella shennongensis Zhang & Yang, sp. nov. is reported from Hubei, China. New record Stiba docerella pristina Brunetti, 1918 from Hainan, China is re-described and illustrated. A key to the known species of Stibadocerella is presented. www.zoobank.org/urn:lsid:zoobank.org:pub:45002F67-2C20-4F3C-A543-81F802EA3900.
Energy spread minimization in a cascaded laser wakefield accelerator via velocity bunching
Zhang, Zhijun,Li, Wentao,Liu, Jiansheng,Wang, Wentao,Yu, Changhai,Tian, Ye,Nakajima, Kazuhisa,Deng, Aihua,Qi, Rong,Wang, Cheng,Qin, Zhiyong,Fang, Ming,Liu, Jiaqi,Xia, Changquan,Li, Ruxin,Xu, Zhizhan American Institute of Physics 2016 Physics of plasmas Vol.23 No.5
New Glycosides from Dracocephalum tanguticum Maxim
Qi Zeng,Hui-zi Jin,Ruijie Chang,Jiangjiang Qin,Xiangrong Cheng,Weidong Zhang 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.12
Four new glycosides, luteolin-7-methoxy-3'-O-(3''-O-acetyl)-β-D-gluco pyranuronic acid-6''-methyl ester (1), benzyl-6-[(2E)-2-butenoate]-β-D-glucopyranoside (2), 2-methoxy-4-(2-propen-1-yl)penyl-6-acetate-β-D-glucopyranoside (3), and 2-methoxy-4-(2-propen-1-yl)penyl-6-[(2E)-2-butenoate]-β- D-glucopyranoside (4), along with benzyl-β-D-glucopyranoside (5), 2-methoxy-4-(2-propen-1-yl)penyl-β-D-glucopyranoside (6), and pectolarigenin (7), were isolated from the whole plant of Dracocephalum tanguticum Maxim. The structures of 1-4 were elucidated by detailed spectroscopic analyses, including HR-ESI-MS and 2D NMR spectroscopic data. The inhibitory effects against nitric oxide production in LPS-stimulated RAW264.7 cells of all seven compounds were also evaluated.
Zhang, Bai-Hua,Cheng, Gui-Yu,Xue, Qi,Gao, Shu-Geng,Sun, Ke-Lin,Wang, Yong-Gang,Mu, Ju-Wei,He, Jie Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.3
Background: Basaloid squamous cell carcinoma of the esophagus (BSCCE) is a rare and distinctive tumor with no standard treatment. This study aimed to explore treatment in relation to prognosis of the disease. Methods: A total of 142 patients with BSCCE that underwent treatment in our hospital from March 1999 to July 2010 were retrospectively analyzed. All patients received surgery, 42 postoperative radiotherapy and 28 patients chemotherapy. Results: There were 26 patients included in stage I, 60 in stage II, 53 in stage III and 3 in stage IV. The clinical symptoms and macroscopic performances of BSCCE did not differ from those of typical esophageal squamous cell carcinoma. Among 118 patients receiving endoscopic biopsy, only 12 were diagnosed with BSCCE. The median survival time (MST) of the entire group was 32 months, with 1-, 3- and 5-year overall survival (OS) of 81.4%, 46.8% and 31.0%, respectively. The 5-year OS of stage I and II patients was significantly longer than that of stages III/IV, at 60.3%, 36.1% and 10.9%, respectively (p<0.001, p=0.001). The MST and 5-year OS were 59.0 months and 47.4% in patients with tumors located in the lower thoracic esophagus, and 27.0 months and 18.1% in those with lesions in the upper/middle esophagus (p=0.002). However, the survival was not significantly improved in patients undegoing adjunctive therapy. Multivariate analysis showed TNM stage and tumor location to be independent prognostic factors. Furthermore, distant metastasis was the most frequent failure pattern, with a median recurrence time of 10 months. Conclusion: BSCCE is an aggressive disease with rapid progression and a propensity for distant metastasis. It is difficult to make a definitive diagnosis via preoperative biopsy. Multidisciplinary therapy including radical esophagectomy with extended lymphadenectomy should be recommended, while the effectiveness of radiochemotherapy requires further validation for BSCCE.
Cheng Chen,Taotao Ding,Zhiqiang Qi,Wei Zhang,Jun Zhang,Juan Xu,Jingwen Chen,Jiangnan Dai,Changqing Chen 대한금속·재료학회 2018 ELECTRONIC MATERIALS LETTERS Vol.14 No.4
The periodically ordered ZnO nanorod (NR) arrays have been successfully synthesized via a hydrothermal approach on thesilicon substrates by templating of the TiO2ring deriving from the polystyrene (PS) nanosphere monolayer colloidal crystals(MCC). With the inverted MCC mask, sol–gel-derived ZnO seeds could serve as the periodic nucleation positions for thesite-specific growth of ZnO NRs. The large-scale patterned arrays of single ZnO NR with good side-orientation can be readilyproduced. According to the experimental results, the as-integrated ZnO NR arrays showed an excellent crystal quality andoptical property, very suitable for optoelectronic applications such as stimulated emitters and ZnO photonic crystal devices.
Prediction of postoperative pancreatic fistula using a nomogram based on the updated definition
Cheng-Xiang Guo,Yi-Nan Shen,Qi Zhang,Xiao-Zhen Zhang,Jun-Li Wang,Shun-Liang Gao,Jian-Ying Lou,Ri-Sheng Que,Tao Ma,Ting-Bo Liang,Xue-Li Bai 대한외과학회 2020 Annals of Surgical Treatment and Research(ASRT) Vol.98 No.2
Purpose: The International Study Group on Pancreatic Fistula’s definition of postoperative pancreatic fistula (POPF) has recently been updated. This study aimed to identify risk factors for POPF in patients having pancreaticoduodenectomy (PD) and to generate a nomogram to predict POPF. Methods: Data on 298 patients who underwent PD from March 2012 to October 2017 was retrospectively reviewed and POPF statuses were redefined. A nomogram was constructed using data from 220 patients and validated using the remaining 78 patients. Independent risk factors for POPF were identified using univariate and multivariate analyses. A predictive nomogram was established based on the independent risk factors and was compared with existing models. Results: Texture of the pancreas, size of the main pancreatic duct, portal vein invasion, and definitive pathology were the identified risk factors. The nomogram had a C-index of 0.793 and was internally validated. The nomogram performed better (C-index of 0.816) than the other most cited models (C-indexes of 0.728 and 0.735) in the validation cohort. In addition, the nomogram can assign patients into low- (less than 10%), intermediate- (10% to 30%), and high-risk (equal or higher than 30%) groups to facilitate personalized management. Conclusion: The nomogram accurately predicted POPF in patients having PD.