Geometrical and Magnetic Properties of Vanadium Clusters Supported on Graphene

Yu Zou,Chang Yong Zhan,Jian-Chun Wu,Li-Ping Zhou,Hai-Xia Da 한국물리학회 2013 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.63 No.2

We report ab-initio calculations of vanadium-cluster Vn(n = 2−5) adsorption on graphene sheets. Geometrical and magnetic properties of various adsorption configurations are studied using firstprinciples density-functional theory with the generalized gradient approximation. The geometrical and magnetic properties of vanadium clusters are found to be size-dependent, and the supported graphene sheet could influence the formation of the vanadium clusters. Low-dimensional Vn cluster configurations could be easily formed when they are absorbed on a graphene sheet, and the combined Vn-graphene systems exhibit a nonmagnetic state, which is the most stable magnetic configuration. Our calculations for the geometrical and the magnetic moment properties of Vn-graphene systems may be of interest for some nanotechnological applications.

Effect of all-trans retinoic acid on casein and fatty acid synthesis in MAC-T cells

Liao, Xian-Dong,Zhou, Chang-Hai,Zhang, Jing,Shen, Jing-Lin,Wang, Ya-Jing,Jin, Yong-Cheng,Li, Sheng-Li Asian Australasian Association of Animal Productio 2020 Animal Bioscience Vol.33 No.6

Objective: Caseins and fatty acids of milk are synthesized and secreted by the epithelial cells of the mammary gland. All-trans retinoic acid (ATRA), an active metabolite of vitamin A, has been shown to promote mammary development. This study was conducted to determine the effect of ATRA on casein synthesis and fatty acid composition in MAC-T cells. Methods: MAC-T cells were allowed to differentiate for 4 d, treated with ATRA (0, 1.0, 1.5, and 2.0 μM), and incubated for 3 d. We analyzed the fatty acid composition, the mRNA expression of casein and fatty acid synthesis-related genes, and the phosphorylation of casein synthesis-related proteins of MAC-T cells by gas chromatography, quantitative polymerase chain reaction, and western blotting, respectively. Results: In MAC-T cells, ATRA increased the mRNA levels of α_S1-casein and β-casein, janus kinase 2 (JAK2) and E74-like factor 5 of the signal transducer and activator of transcription 5 β (STAT5-β) pathway, ribosomal protein S6 kinase beta-1 (S6K1) and eukaryotic translation initiation factor 4E binding protein 1 of the mammalian target of rapamycin (mTOR) pathway, inhibited the mRNA expression of phosphoinositide 3-kinase and eukaryotic initiation factor 4E of the mTOR pathway, and promoted the phosphorylation of STAT5-β and S6K1 proteins. Additionally, ATRA increased the de novo synthesis of fatty acids, reduced the content of long-chain fatty acids, the ratio of monounsaturated fatty acids to saturated fatty acids (SFA), the ratio of polyunsaturated fatty acids (PUFA) to SFA, and the ratio of ω-6 to ω-3 PUFA. The mRNA levels of acetyl-CoA carboxylase 1, fatty acid synthase, lipoprotein lipase, stearoyl-CoA desaturase, peroxisome proliferator-activated receptor gamma, and sterol regulatory element-binding protein 1 (SREBP1) were enhanced by ATRA. Conclusion: ATRA promotes the synthesis of casein by regulating JAK2/STAT5 pathway and downstream mTOR signaling pathway, and it improves the fatty acid composition of MAC-T cells by regulating SREBP1-related genes.

VHL Gene Mutation Analysis of a Chinese Family with Non-Syndromic Pheochromocytomas and Patients with Apparently Sporadic Pheochromocytoma

Zhang, Bin,Qian, Jing,Chang, De-Hui,Wang, Yang-Min,Zhou, Da-Hai,Qiao, Gou-Mei Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.5

Objective: The Von Hippel-Lindau syndrome (VHLD), an inherited neoplastic syndrome predisposing to central nervous system hemangioblastoma (CNS), pheochromocytoma (PCC), renal cell carcinoma(RCC), retinal hemangioma (RA) and renal cysts, is caused by mutations or deletions of the VHL tumor-suppressor gene. To assess VHL genotype-phenotype correlations with function of pVHL a gene mutation analysis of members in a Chinese family with non-syndromic PCCs and individuals with apparently sporadic pheochromocytoma (ASP) was performed. Materials and Methods: DNA samples of 20 members from the Chinese family with non-syndromic PCCs and 41 patients with ASP were analyzed by polymerase chain reaction and direct sequencing, confirmed by Taqman probe. Results: Three novel mutations (H125P, 623(^TTTGTtG) and R120T) were identified in the Chinese family and in 3 among 41 ASP patients. The mutations were all located in exon 2 of VHL gene encoding ${\beta}$-domain of pVHL. The tumor type in H125P carriers and R120T carriers was VHL type 2C. And 623(^TTTGTtG) carriers presented VHL type 2B or type 2C. Conclusions: VHL gene abnormalities were identified in the Chinese family with non-syndromic PCCs and patients with APS, resulting in dysfunction of pVHL. H125P and R120T could be associated with VHL type 2C, while 623(^TTTGTtG) might be linked with VHL type 2B or type 2C. Not only is the genetic analysis helpful for early diagnosis and treatment of patients with VHLD, it is also benefitial for research intoVHLD pathogenesis.

Emergence of robust 2D skyrmions in SrRuO₃ ultrathin film

Bongju Kim,Byungmin Sohn,Hai Huang,Sang-Jun Lee,Se Young Park,Taeyang Choi,Hua Zhou,Seo Hyoung Chang,Jung Hoon Han,Jun-Sik Lee,Changyoung Kim 한국자기학회 2019 한국자기학회 학술연구발표회 논문개요집 Vol.29 No.1

Efficacy of Pap Test in Combination with ThinPrep Cytological Test in Screening for Cervical Cancer

Chen, Hua,Shu, Hui-Min,Chang, Zhou-Lin,Wang, Zhi-Feng,Yao, Hai-Hong,Zhu, Hong-Mei,Lu, Tian-Mei,Ma, Qiang-Yan,Yang, Bin-Lie Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.4

Background: Our aim was to investigate the efficacy of the Pap test in combination with the ThinPrep cytological test (TCT) in screening for cervical cancer in China. Design: From March 2006 to October 2008, 988 women with the mean age $46.4{\pm}10.5$ years (range, 23-80 years) were recruited to receive cervical cancer screening. Pap test results ${\geq}$ grade III and TCT findings ${\geq}$ ASCUS/AGUS were considered abnormal. Subjects with a Pap test result ${\geq}$ grade IIb received TCT. Colposcopy and biopsies were performed in all participants, and final diagnosis was based on pathological findings. The sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV) and Youden index for predicting CIN I or above were determined. Results: The sensitivity, specificity, PPV, NPV and Youden index of the Pap test were 43.1%, 97.2%, 70.0%, 91.9%, and 40.3%, respectively. The same values for TCT in predicting CIN were 80.0%, 63.2%, 16.0%, 97.3%, and 43.2%, respectively. The two tests in combination gave values for predicting CIN of 64.8%, 87.6%, 43.6%, 94.4%, and 53.5%, respectively. Combined testing exhibited the highest Youden index (53.4%). Conclusion: The Pap test with a reduced threshold in combination with the TCT has high sensitivity and high specificity in screening for cervical cancer.

Chemical composition and in vitro digestibility of corn stover during field exposure and the fermentation characteristics of silage prepared with microbial additives

Jun Lei Gao,Peng Wang,Chang-Hai Zhou,Ping Li,Hong Yu Tang,Jiabao Zhang,Yimin Cai 아세아·태평양축산학회 2019 Animal Bioscience Vol.32 No.12

Objective: To effectively use corn stover resources as animal feed, we explored the chemical composition and in vitro digestibility of corn stover during field exposure and the fermentation characteristics of silage prepared with lactic acid bacteria (LAB) and cellulase. Methods: Corn ears including the cobs and shucks were harvested at the ripe stage. The corn stover was exposed in the field under natural weather conditions. Silages were prepared after 0, 2, 4, 7, 15, 30, and 60 d of exposure. Corn stover was chopped into approximately 1 to 2 cm lengths and then packed into 5 liter plastic silos. The ensiling density was 550.1±20.0 g/L of fresh matter, and the silos were kept at room temperature (10°C to 25°C). Silage treatments were designed as follows: without additives (control), with LAB, with cellulase, and with LAB+ cellulase. After 45 d of fermentation, the silos were opened for chemical composition, fermentation quality and in vitro digestion analyses. Results: After harvest, corn stover contained 78.19% moisture, 9.01% crude protein (CP) and 64.54% neutral detergent fiber (NDF) on a dry matter (DM) basis. During field exposure, the DM, NDF, and acid detergent fiber (ADF) contents of corn stover increased, whereas the CP and water-soluble carbohydrate contents and in vitro digestibility of the DM and CP decreased (p<0.05). Compared to the control silage, cellulase-treated silage had lower (p<0.05) NDF and ADF contents. The pH values were lower in silage treated with LAB, cellulase, or LAB+cellulase, and lactic acid contents were higher (p<0.05) than those of the control. Silage treated with cellulase or LAB+cellulase improved (p<0.05) the in vitro DM digestibility (IVDMD) compared to that of the control or LAB-treated silage. Conclusion: Corn stover silage should be prepared using fresh materials since stover nutrients are lost during field exposure, and LAB and cellulase can improve silage fermentation and IVDMD.

Association of Chemotherapy-induced Leucopenia with Treatment Outcomes in Advanced Non-small-cell lung Cancer Cases Receiving the NP Regimen

Huang, Cheng-Suo,Liu, Lin,Liu, Jie,Chen, Zhen,Guo, Jun,Li, Chang-Zheng,Zhou, Deng-Guang,Wang, Zhe-Hai Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.9

Background: Chemotherapy induced leutropenia has been shown to be associated with improved treatment outcomes in selected solid tumors. We studied the association of chemotherapy induced leutropenia with treatment related outcomes in advanced non-small-cell lung cancer. Methods: This is a prospective analysis of patients receiving chemotherapy for advanced NSCLC at the Shandong Cancer Hospital from 2005-07.The chemotherapy included cisplatin $35mg/m^2$, IV on $d_{1,2}$ and vinorelbine $25mg/m^2$ IV on $d_{1,8}$ every 21 days. Patients were stratified into three groups (A) those experiencing grades 0 leucopenia, group (B) grades 1-2 and group (C) grades 3-4. The outcomes studied were response rate (RR), disease control rate (DCR), and time to progression (TTP). Results: 128 patients were studied. The RRs in groups A, B and C were 30.8%, 56.8% and 71.4%, respectively, p=0.010. The DCRs were 61.5%, 83.8% and 92.9%, respectively, p=0.009 and the median TTPs were 150 days (95%CI: 91-209), 189 days (95%CI: 181-197) and 207 days (95%CI: 172-242), p=0.009. The differences in RR and TTP were significant. In patients whose CIL kept on 10 days at least, the TTP was significantly prolonged, p=0.0213, and the same was the case for those experiencing grades 1-2 leucopenia and ECOG 0, p=0.0412. Conclusions: Occurrence of CIL correlated with RR and TTP in patients with advanced NSCLC receiving cisplatin and vinorelbine chemotherapy, especially in patients experiencing grades 1-2 leucopenia and ECOG 0, and the same for those with CIL persisting for 10 days at least. CIL could be a biological measure of drug activity and a marker of efficacy.

Lithium chloride's inhibition of 3T3-L1 cell differentiation by regulating the Wnt/β-catenin pathway and enhancing villin 2 expression

Chi, Yu-Yang,Shen, Jing-Lin,Zhang, Jing,Shan, An-Shan,Niu, Shu-Ling,Zhou, Chang-Hai,Lee, Hong-Gu,Jin, Yong-Cheng Korean Society of Food Science and Technology 2016 Food Science and Biotechnology Vol.25 No.4

The aim of this study is to reveal the relation among villin 2, $Wnt/{\beta}-catenin$, and adipogenesis by adding appropriate lithium chloride (LiCl). The study comprises three parts: the selection of LiCl concentration, the effect of LiCl on adipocyte differentiation during and after differentiation induction. By comprehensively analyzing the results of the experiments, we proved that LiCl can inhibit adipocyte differentiation and enhance villin 2 and ${\beta}-catenin$ expressions not only during differentiation induction but also after it. Moreover, villin 2 has a significant impact on ${\beta}-catenin$. We suggest that villin 2 may participate in $Wnt/{\beta}-catenin$ signaling.

Lithium chloride’s inhibition of 3T3-L1 cell differentiation by regulating the Wnt/β-catenin pathway and enhancing villin 2 expression

Yu-Yang Chi,Jing-Lin Shen,Jing Zhang,Anshan Shan,Shu-Ling Niu,Chang-Hai Zhou,이홍구,Yong-Cheng Jin 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.4

The aim of this study is to reveal the relation among villin 2, Wnt/β-catenin, andadipogenesis by adding appropriate lithium chloride (LiCl). The study comprises three parts: theselection of LiCl concentration, the effect of LiCl on adipocyte differentiation during and afterdifferentiation induction. By comprehensively analyzing the results of the experiments, we proved thatLiCl can inhibit adipocyte differentiation and enhance villin 2 and β-catenin expressions not only duringdifferentiation induction but also after it. Moreover, villin 2 has a significant impact on β-catenin. Wesuggest that villin 2 may participate in Wnt/β-catenin signaling.