성장생물학 : 체지방 감소 다클론 후보 항체가 비거세돈의 체중, 분 소화율 및 혈액 대사물질에 미치는 영향

최창본 ( Chang bon Choi ),오영균 ( Young Kyoon Oh ),백경훈 ( Kyung Hoon Baek ),홍성구 ( Seong Koo Hong ),최창원 ( Chang Weon Choi ),조성백 ( Sung Back Cho ) 한국동물자원과학회 ( 구 한국축산학회 ) 2010 한국축산학회지 Vol.52 No.5

본 연구는 비거세돈 12두를 이용하여 돼지 복강 및 피하지방 감소 후보 항체를 생체 주사 처리 시 체중, 분 소화율 및 혈액 대사물질 등 영양생리대사에 미치는 영향을 조사하고자 실시하였다. Choi 등(2010)에서 개발된 돼지 복강 및 피하지방 감소 후보 항체를 피하 주사하였을 때 주사 후 4주까지 평균 체중은 수치적 증가가 발생했으나(P>0.05), 전 기간에 걸쳐 수치적 증가를 보여 항체의 처리로 인한 영향보다는 돼지의 일반적인 성장에 기인하는 것으로 판단된다. 항제 주사 처리 시 건물, 조단백질, 조지방 및 조섬유의 분 소화율은 항체 주사 전, 후 및 시험구 간의 통계적 유의성이 나타나지 않았다(P>0.05). 조회분 소화율의 경우 항체 주사 후 2주째 control에서 감소를 및 4주째 NAb에서 증가를 보였으나(P< 0.05), AAb 및 SAb에서는 유의적 변화가 없었다. 혈액 대사물질의 변화에서는 항체 주사 후 4주의 AAb 및 SAb에서 BUN 농도가 유의적으로 증가하는 것으로 나타났다(P<0.05). 하지만, 후보 항체 주사 전에서도 유사한 패턴으로 유의적 증가를 보여 항체 처리에 의한 차이보다는 개체간의 차이로 사료된다. 혈액 내 glucose 및 TG 농도는 후보 항체 처리에 의한 유의적 차이는 나타나지 않았다(P>0.05). 체지방 함량과 밀접한 관계에 있는 혈액 내 TC 농도에서는 control 대비 후보 항체 주사 후 4주차 AAb 및 SAb에서 유의적 감소가 나타나(P<0.05), 체지방 감소가 일어나고 있음을 간접적으로 추론할 수 있다. 이상의 결과를 볼 때, 본 연구에서 이용된 AAb 및 SAb는 in vivo 영양생리대사에 부정적 영향을 미치지 않는 안전한 항체로 판단된다. Twelve pigs were used to investigate the effects of polyclonal antibody candidate against abdominal (AAb) and subcutaneous adipocyte membrane proteins (SAb) on body weight, fecaldigestibility and blood metabolites. When AAb and SAb developed by Choi et al. (2010) were injected to pigs, the numerical increase in BW (body weight) occurred at 4 weeks post-treatment, but BW for an entire period was also increased, indicating that the BW increase may not be affected by the antibodies injection. Antibodies treatment did not affect (P>0.05) fecal digestibility of dry matter, crude protein, crude fat and crude fiber. Fecal digestibility of crude ash for control (no treatment) at 2 weeks decreased, and that for non-immunized serum treatmentgroup at 4 weeks post-treatment increased, respectively (P<0.05). However, fecal digestibility of crude ash for AAb and SAb groups did not significantly change. At 4 weeks after the antibodies treatment, blood urea N concentration for AAb and SAb groups was significantly increased (P<0.05). However, these increases may not be caused by the antibodies treatment because similar pattern in blood urea N concentration occurred before the antibodies treatment. Antibodies treatment did not affect concentration of plasma glucose and triglycerides (P<0.05). Compared with control, concentration of plasma total cholesterol for AAb and SAb groups at 4 weeks post-treatment was significantly (P<0.05) decreased. This may suggest that body fat reduction possibly occurs. In conclusion, the AAb and the SAb developed by Choi et al. (2010) may have safety in nutritional physiological metabolism in pigs. Further study on in vivo fat reduction of the antibodies against abdominal and subcutaneous adipocytes of pigs should be required for fat-reduced pork production.

지가의 공간분포 분석을 통한 부산시 도시공간구조해석에 관한 연구

최창환(Choi Chang Hwan),이동본(Lee Dong Bon) 한국부동산학회 2005 不動産學報 Vol.25 No.-

1. CONTENTS (1) RESEARCH OBJECTIVES This study investigates the urban spatial structure which analyzes land distribution into its price in Busan. The land is limited resources, so the price and property of land are changed. It affects the urban spatial structure. Therefore it is important that the urban spatial structure was explained by using the price of land. (2) RESEARCH METHOD cluster analysis (3) RESEARCH RESULTS As result we realized that they have no relations between density of population and the price of land, but between the price of land and the distance from downtown have relations. The relations explained that farther place from downtown is lower price of land than a location which is near to downtown. 2. RESULTS The study reaches the two conclusion : first, the spatial structure of Busan was divided into two parts of inner functional space, a part of outer functional space and 7 parts of outer area. The second conclusion of the study is that the price of outer land is more expansive than center of city in some cases. 3. KEY WORDS the piece of land, urban spatial structure, Cluster Analysis, a economic zone

면양을 이용한 돼지 지방세포 원형질막 단백질 특이 항체의 생산

최창본,권은진,이명진 THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 1998 Journal of biomedical laboratory sciences Vol.4 No.1

본 연구는 돼지 지방세포 원형질막 단백질에 대한 항체를 면양에서 생산하고 생산된 항체의 역가 및 조직특이성을 조사하기 위하여 실시되었다. 지방세포, 뇌, 심장, 신장, 간장 및 비장으로부터 원형질막 단백질을 추출하였으며, 그중 지방세포로부터 분리한 원형질막 단백질을 면양(체중 40㎏)에 3주 간격으로 3회 면역접종시켰다. 면역접종 전, 3차 면역접종 후 10일 (AS-1), 12일 (AS-2) 및 14일 (AS-3)째에 각각 면양의 경정맥으로부터 혈액을 채취하여 혈청을 채취하여 혈청을 분리하였다. 항체의 역가 및 기타 조직과의 교차반응성은 enzyme-linked immunosorbent assay (ELISA)로 측정하였다. 면양에서 생산된 돼지 지방세포 원형질막 단백질에 대한 항혈청은 지방세포 원형질막 단백질과 강한 항원-항체 반응을 나타내었다. 항혈청의 교차반응성을 조사한 결과, 기타 조직의 원형질막 단백질과는 매우 미약한 반응을 나타낸 반면 지방세포 원형질막 단백질과는 강한 반응을 나타내었다. 이러한 항혈청의 지방세포 원형질막 단백질과의 조직특이적인 반응은 anti-sheep immunoglobulin Ghorseradish peroxidase conjugate를 2차 항체로 이용한 immunoblot에 의해서도 재확인 되었다. 이상의 결과, 면양으로부터 생산된 돼지 지방세포 원형질막 단백질에 대한 항체는 높은 역가를 지니고 있었으며, 지방세포 원형질막 단백질에 특이적으로 작용함을 알 수 있었다. The objectives of this study were to produce polyclonal antibody to adipocyte plasma membrane (APM) proteins isolated from pig, and to investigate its tissue specificity. Plasma membrane proteins from adipocyte, brain, heart, kidney, liver and spleen were isolated using a self-forming Percoll gradient. Sheep (40㎏) was immunized three times at three week interval with the purified APM proteins. Blood was taken from non-immunized sheep (NS) and from immunized sheep at 10 (AS-1), 12 (AS-2), and 14 (AS-3) days after the third immunization. Antisera titers and cross-reactivity against other tissues were determined by enzyme-linked immunosorbent assay (ELISA). Antisera reacted strongly to APM proteins showing detectable amounts of antibody at 1:81,000 dilution. And antisera showed much stronger reactivity to APM proteins than any other tissue plasma membrane proteins. Furthermore, tissue specificity of antisera against APM was reconfirmed by immunoblotting using anti-sheep immunoglobulin Ghorseradish peroxidase conjugate as a secondary antibody. Antisera to APM proteins showed adipocyte specificity compared with other tissues. In conclusion, polyclonal antibody against APM proteins isolated from pig was developed successfully in our laboratory, and these antisera showed tissue specificity with APM.

BC₃H1 혈관평활근세포의 증식 및 분화과정에 있어서 Polyamine 대사의 역할에 관한 연구

최상현,문창택,민본홍,전보권,천연숙 고려대학교 의과대학 1992 고려대 의대 잡지 Vol.29 No.3

The influences of spermine (SM) and DFMO. an irreversible ODC-inhibitor, on the polyamine metabolism, CK-activity, [^(3)H]-thymidine DNA synthesis, and isoactin mRNA expression of BC_(3)H1 in culture were studied, with the references of phosphodiesterase(PDE)-inhibitors including isobutyl-methylxanthine (IBMX) and KR30075. BC_(3)Hl cells cultured in 10% fetal bovine serum (FBS)-DMEM for 24 hrs (the first : pre-drug phase) were cultivated in 10% FBS-DMEM added with one of the test drugs for further 96 hrs(the second proliferation phase), and then continue to grow in 2% FBS-DMEM for 48 hrs (the third : differentiation phase). The putrescine content of BC_(3)Hl harvested after the first phase was relatively higher in comparison with those of the other phases and was gradually decreased through the full phases of culture, and the spermine content was little changed. The spermidine (SD) content was. unlike other polyamines, increased by 26.5% at the end of the second phase, but that SD increase was moderately attenuated by PDE-inhibitors, particularly KR30075, and furthermore. the SD content of BC_(3)Hl cultured in the presence of DFMO, unlike other polyamines. showed the marked and gradual decrease throughout the full phases of culture. The [^(3)H]-thymidine DNA synthesis during the third : differentiation phase was not affected by IBMX and slightly inhibited by KR30075, but was significantly inhibited by SM plus DFMO or each of them. And the creatine kinase (CK) activity gradually increased in advancing with culture duration was not changed by PDE-inhibitors. but both SM and DFMO significantly enhanced the increase of CK activity. The synthesis of BC_(3)Hl α-actin and β-/ γ-actin mRNAs was significantly enhaced by DFMO, and the DFMO-induced enhancement was dramatically inhibited by SM. And PDE-inhibitors and SM little affected the synthesis of α-actin mRNA, but PDE-inhibitors attenuated the synthesis of β-/ γ-mRNAs, especially in the second phase. These results suggest that polyamines have a pivotal role in the proliferation and differentiation of BC_(3)Hl cells, that the enhancement induced by DFMO of expression of VSM isoactin gene seems to be an attractive subject to be studied in future, and that PDE-inhibitors may inhibit the synthesis of VSM β-/ γ-actin mRNAs.

Isonitrosoethylacetoacetate Imine 리간드의 니켈(II) 착물 합성 및 구조 특성

김인환,구본창,최강열,김상태,이만호 大邱大學校附設 基礎科學硏究所 1993 基礎科學硏究 Vol.10 No.-

Nickel(lI) Complexes of the type Ni(IEAA-NH)(IEAA-NR) (R = H₁, CH₃, C₂H_(5), n-C₃H_(7), n-C₄H_(9), or CH₂C_(6)H_(5)), where IEAA-NH and IEAA-NR represents isonitrosoethylacetoacetate imine and its N-alkyl derivative, respectively, have been prepared. And the structure of the complexes have been studied by elemental analyses, electronic, infrared,1H and 13C-NMR spectra. It has been determined that IEAA-NH ligand coordinates to nickel through the nitrogen of imine group and the oxygen of isonitroso group to form 6-membered chelate ring and IEAA-NR coordinates to nickel through the nitrogens of imino group and isonitroso group to form 5-membered chelate ring in the square-planar Ni(IEAA-NH)(IEAA-NR) complexes.

高血壓性 腦卒中(中風)에 對한 東西醫學的 疫學硏究

鄭在赫,具本泓,張仁圭,崔栖영,柳東俊 中央醫學社 1983 中央醫學 Vol.45 No.3

Epidemiological studies were done on the 162 hypertensive cerebrovascular accid-ents cases admitted in the affiliated Oriental Medicine School Hospital Kyung Hee University in 1983 and the following results were obtained. 1. The male to female ratio of the patients was 1. 3 : 1 and the age distribution frequency was as follows; fifties, forties, seventies and thirties(Table 2). 2. In the C.T.A., there were 119 cases of cerebral thrombosis(73.5%) and 45 cases of intracerebral hemorrhage (26.5%). The ratio was 2.8 : 1. In the former the male to female ratio was 1.3 : 1, in the latter it was 1.2 : 1(Table 3). 3. There were considerable numbers of recurrent cases as shown in Table 4. The first attack was noted in 125 cases(77.2%), the 2nd attack 30 cases(18.5%), the 3rd attack 6 cases(3.1%), 4th attack 1 case(0.6%). The male to female ratio was as follows. In the first attack it was 1. 3 : 1, in the 2nd attack 1.1 : 1, the 3rd attack 5.0: 1, the 4th attack was noted only in one male case. 4. The occupational frequency order was as follows(Table 5). Unemployed initi-ated with 45 cases(27.8%), home care 21 cases, Unspecified 19 cases, office worker, business men, farmers and public official etc. 5. The regional distribution was as follows(Table 6). Seoul city led with 102 cases (63. 0%) then it was followed by Kyung-Gi, Chung-Cheong, Kyung-Sang, Kang-Won and Jon-Ra province. 6. The systolic pressures at admission were disclosed as follows(Table 7). There were 42 cases(male 20 cases-12.3%, female 22 cases-13.6%) of systolic hypertens-ive over the WHO standard of 160mmHg, the mean value of systolic pressure was 145mmHg. The diastolic pressures at admission were shown as follows(Table 8). There were 92 cases(56.8 %) (male 48 cases-29.6%' female 44 cases-27.2%) of diastolic hypertension over the WHO standard of 95mmHg, the mean value of diastolic pressure was 98mmHg. 7. The patient's favors to salty food, animal fatty food, cigarettes and alcoholic beverage were examined (Table 9). Salty' foods were favored by 78 cases(48.2%), animal fatty foods by 78 cases(48.2%), cigarettes by 64 cases(39.5%) and alcohol imbibers were noted in 77 cases(47.5%) as shown in table 9. 8. The family history of C. V.A and hypertension was studied (Table 10). In C. V.A there were 28 cases (17.3%) with parental history, 12 cases (7.4%) with siblings history and 7 cases (4.3%) with other relatives, and in hypertension there appeared 14 cases(8.6%) with parental history, 9 cases(5.6%) with sibling history and 6 cases(3.7%) with other relatives. 9. In the past history, hypertension led with 124 case s(76.5°%), then C.V.A 37 cases(22.9%) and diabetes mellitus 33 cases(20.4%)(Table 11). 10) In treatment the frequently used herbal recipes were Gamichungshimtang(), BoSimgunbitang( ), Kyuntongdudamtang( ) in 15% of the cerebral hemorrhage cases, respectively; Camichungshimtang( ) in 20% Yangkyeuksan(), Chowishungchumtang(), Yukmichihwangt-ang() in 6%, respectively, in cerebral thrombosis. The most frequently used acupuncture points in C. V. A were shown in Table 12. Taechung(), Habgok ( ), Joksamri(), Oekwan(), Chungjue( ), Jokimueb.(), Kokji(), Taebak() were frequency order used in C.V.A of both cerebral thrombosis and hemorrhage. 11. In admitted C.V.A patient care, it was stressed that the Oriental Medical Hospital plays important roles and, accordingly, the propriety of East-West medical cooperation was addressed. 12. Through classified disease distribution of admitted patients in the Oriental Medicine Hospital the epidemiological epitome of cerebrovascular accidents and other diseases was presented. * East-West medicine denotes a methodology of approaching a creative medicine through cooperation between East-West medicines.

高血壓性 腦卒中(中風)에 對한 東西醫學的 疫學硏究

鄭在赫,具本泓,張仁圭,崔栖瀅,柳洞俊 WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 1983 東西醫學硏究所 論文集 Vol.2 No.1

Epidemiological studies were done on the 162 hypertensive cerebrovascular accidents cases admitted in the affiliated Oriental Medicine School Hospital Kyung Hee University in 1983 and the following results were obtained. 1. The male to female ratio of the patients was 1.3:1 and the age distribution frequency was as follows; fifties, forties, seventies and thirties(Table 2.) 2. In the C.V.A., there were 119 cases of cerebral thrombosis(73.5%) and 45 cases of intracerebral hemorrhage(26.5%). The ratio was 2.8:1. In the former the male to female ratio was 1.3:1, in the latter it was 1.2:1(Table 3). 3. There were considerable numbers of recurrent cases as shown in Table 4. The first attack was noted in 125 cases(77.2%), the 2nd attack 30 cases(18.5%), the 3rd attack 6 cases(3.1%) , 4th attack 1 case(0.6%). The male to female ratio was as follows. In the first attack it was 1.3:1, in the 2nd attack 1.1:1, the 3rd attack 5.0:1, the 4th attack was noted only in one male case. 4. The occupational frequency order was as follows(Table 5). Unemployed initiated with 45 cases(27.8%), home care 21 cases, Unspecified 19 cases, office worker, business men, farmers and public official etc. 5. The regional distribution was as follows(Table 6). Seoul city led with 102 cases (63.0%) then it was followed by Kyung-Gi, Chung-Cheong, Kyung-Sang, Kang-Won and Jon-Ra province. 6. The systolic pressures at admission were disclosed as follows(Table 7). There were 42 cases(male 20 cases-12.3%, female 22 cases-13.6%) of systolic hypertensive over the WHO standard of 160mmHg, the mean value of systolic pressure was 145mmHg. The diastolic pressures at admission were shown as follows(Table 8). There were 92 cases(56.8%)(male 48 cases-29.6%, female 44 cases-27.2%) of diastolic hypertension over the WHO standard of 95mmHg, the mean value of diastolic pressure was 98mmHg. 7. The patient's favors to salty food, animal fatty food, cigarettes and alcoholic beverage were examined(Table 9). Salty foods were favored by 78 cases(48.2%), animal fatty foods by 78 cases(48.2%), cigarettes by 64 cases(39.5%) and alcohol imbibers were noted in 77 cases(47.5) as shown in table 9. 8. The family history of C.V.A and hypertension was studied (Table 10). In C.V.A there were 28 cases(17.3%) with parental history, 12 cases(7.4%) with siblings history and 7 cases(4.3%) with other relatives, and in hypertension there appeared 14 cases(8.6%) with parental history, 9 cases(5.6%) with sibling history and 6 cases(3.7%) with other relatives. 9. In the past history, hypertension led with 124 cases(76.5%), then C.V.A 37 cases(22.9%) and diabetes mellitus 33 cases(20.4%)(Table 11). 10. In treatment the frequently used herbal recipes were Gamichungshimtang(加味淸心湯), Bosimgunbitang(補心健脾湯), Kyuntongdodamtang(腱痛導痰湯) in 15% of the cerebral hemorrhage cases, respectively, Gamichungshimtang(加味淸心湯) in 20%, Yangkyeuksan(凉膈散), Chowisungchongtang(調胃升淸湯), Yukmichihwangtang(六味地黃湯) in 6%, respectively, in cerebral thrombosis. The most frequently used acupuncture points in C.V.A were shown in Table 12. Taechung(太衡) , Habgok(合谷), Joksamri(足三里), Oekwan(外關), Chungjue(中渚), Jokimeub(足姙泣), Kokji(曲池), Taebak(太白) were frequency order used in C.V.A of both cerebral thrombosis and hemorrhage. 11. In admitted C.V.A patient care, it was stressed that the Oriental Medical Hospital plays important roles and, accordingly, the propriety of East-West medical cooperation was addressed. 12. Through classified disease distribution of admitted patients in the Oriental Medicine Hospital the epidemiological epitome of cerebrovascular accidents and other diseases was presented.

품종 및 성별에 따른 쇠고기의 지방산조성 비교

김대곤,성삼경,정근기,최창본 대구산업정보대학 1995 논문집 Vol.9 No.2

Dae Gon The study was conducted to investigate the effect of breed, sex on fatty acid compostition. Hanwoo and Holstein used, in the study were classified by sex. The results are summarized as follows ; Oleic acid and unsaturated fatty acids(UFA) percentages of total fatty acids in Hanwoo were higher than in Holstein, but palmitic acid and saturated fatty acids(SFA) were lower in Hanwoo. Oleic acid in steers of Hanwoo and Holstein tended to increase compared with bulls. Oleic acid in rib loin and top round were considerably higher in Hanwoo steers than in Holstein steers. In Hanwoosteers, oleic acid and UFA tended to increased while stearic acid and SFA decreased as marbling degree increased.

Effects of Rice Bran, Flax Seed, and Sunflower Seed on Growth Performance, Carcass Characteristics, Fatty Acid Composition, Free Amino Acid and Peptide Contents, and Sensory Evaluations of Native Korean Cattle (Hanwoo)

Choi, Chang Bon,Kwon, Hana,Kim, Sung Il,Yang, Un Mok,Lee, Ju Hwan,Park, Eun Kyu Asian Australasian Association of Animal Productio 2016 Animal Bioscience Vol.29 No.2

This study was conducted to evaluate the effect of dietary supplementation with rice bran, flax seed, or sunflower seed to finishing native Korean cattle (Hanwoo) on growth performances, carcass characteristics, fatty acid composition, free amino acid and peptide contents, and sensory evaluations of Longissimus muscle (LM). A total of 39 Hanwoo steers (average age of 22.2 mo and average body weight (BW) of 552.2 kg) were randomly divided into Control, rice bran (RB), flax seed (FS), or Sunflower seed (SS) groups. The steers were group fed for 273 d until they reached an average age of 31.2 mo. Final BW was 768.2, 785.8, 786.2, and 789.0 kg, and average daily gain was 0.79, 0.85, 0.82, and 0.84 kg for the Control, RS, FS, and SS groups, respectively (p>0.05). Fat thickness of the FS group (19.8 mm) was greater (p<0.05) than that of the other groups. Final yield grade converted into numerical values was 2.0 for the RB group, 1.7 for the Control and SS groups, and 1.4 for the FS group. Marbling degrees for the Control, SS, RB, and FS groups were 5.3, 5.1, 4.7, and 4.6, respectively. Percentages of palmitic acid ($C_{16:0}$), stearic acid ($C_{18:0}$), and arachidic acid ($C_{20:0}$) in the LM were not different among the groups. Palmitoleic ($C_{16:1}$) acid was higher (p<0.05) in the SS group. The concentration of oleic acid was highest (p<0.05) in the Control group (47.73%). The level of linolenic acid ($C_{18:3}$) was 2.3 times higher (p<0.05) in the FS group compared to the other groups. Methionine concentration was (p<0.05) higher in FS (1.7 mg/100 g) and SS (1.2 mg/100 g) steers than in the Control or RB groups. Glutamic acid and ${\alpha}$-aminoadipic acid (${\alpha}$-AAA) contents were (p<0.05) higher in the FS group compared to the other groups. LM from the FS group had numerically higher (p>0.05) scores for flavor, umami, and overall palatability in sensory evaluations. In conclusion, supplementation of flax seed to diets of finishing Hanwoo steers improved sensory evaluations which might have been caused by increases in flavor related amino acids such as methionine, glutamic acid and ${\alpha}$-AAA and peptides, anserine and carnosine, and their complex reactions.

Presence of an Albumin-Like Protein on the Plasma Membrane of Adipocytes Isolated from Korean Cow

Choi, Chang-Bon,Rhee, Seung-Keun Korean Society for Biochemistry and Molecular Biol 1998 Journal of biochemistry and molecular biology Vol.31 No.6

The main function of adipocytes in various species is to store nutrient energy in the form of triglycerides, and this function may he closely related with hormonal signaling through the plasma membrane of adipocytes. Using SDS-PAGE, two-dimensional gel electrophoresis, and a membrane biotinylation technique, we have identified a 55KDa protein (55K protein) from the plasma membrane fraction of adipocytes, with an isoelectric point (pI) of 8.1-8.3. However, this 55K protein was not observed with a two-dimensional gel electrophoresis carried out on plasma membrane fractions prepared from the liver, heart, and kidney tissues. An analysis of the 12 amino acids sequence at the N-terminal of the 55K protein showed that it has a similar sequence to that of bovine serum albumin.