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백서 대뇌 피질에서 허혈에 의한 [^3H]5-Hydroxytryptamine의 유리
장영문,송은석,노시운,김성수,김재천,김기원 의과학연구소 1995 全北醫大論文集 Vol.19 No.1
In an attempt to elucidate the mechanisms for ischemia-induced release of neurotransmitters in vitro. I examined the ischemia-induced release of [^3H]5-hydroxytryptamine(5-HT) from cerebral cortex of the rat. Ischemia, deprivation of oxygen and glucose, induced significant release of [^3H]5-HT(about 6% of total tissue content) from the cerebral cortex in vitro. This ischemia-induced release of [^3H]5-HT from the cerebral cortex was significantly attenuated by TTX(1μM), Mg^2+(1.2mM), MK-801(10μM), ketamine(10μM), NMDA receptor antagonists, DNQX(30μM), a kainate/AMPA receptor antagonist, or carbetapentane(30μM), an inhibitor of glutamate release. Dantrolene(30μM) and ryanodine(100μM), inhibitors of intracellular Ca^2+ release, flunarizine(5μM) and ω-conotxin(100μM), inihbitors of N-type Ca^2+ channels, signficantly attenuated the ischemia-induced release of [^3H]5-HT, but verapamil(5μM), and inhibitor of L-type Ca^2+ chnnels, did not. Fluoxetine(1μM), a relatively selective 5-HT transporter blocker, significantly inhibited the ischemia-induced release of [^3H]5-HT. These results suggest that ischemia-evoked release of norepinephrine was caused by release of glutamate via activation of NMDA and non-NMDA receptors, and that CA^2+-dependent and -independent release processes are underlying in this phenomenon.
Chang, Hyun-Joo,Kim, Young Sup,Ryu, Shi Yong,Chun, Hyang Sook The Korean Society for Applied Biological Chemistr 2013 Applied Biological Chemistry (Appl Biol Chem) Vol.56 No.4
Neuroprotective activities of 176 phytochemicals were investigated against oxygen-glucose deprivation in vitro using primary cortical neuron culture. Diterpenes (taxol and taxol C) were the most potent neuroprotective compounds. $EC_{50}$ of taxol and taxol C were 0.402 and $0.452{\mu}M$, respectively. This result supports the use of an in vitro model as initial screening for neuroprotective candidates, which warrants further testing in animal models.
Zhao, Chang,Wang, Xu-Jie,Wang, Song,Feng, Wei-Hua,Shi, Lei,Yu, Chun-Peng Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.5
Purpose: To assess the effect and safety of lobaplatin combinated floxuridine /pirarubicin in transcatheter hepatic arterial chemoembolization(TACE) of unresectable primary liver cancer. Patients and Methods: TACE combined with the chemotherapy regimen was used to treat 34 unresectable primary liver cancer patients. DSA/MRI/CT/blood routine examinations were used to evaluate short term activity and toxicity after 4-5 weeks, the process being repeated if necessary. Results: Among the 34 cases, 1 (2.9%) showed a complete response, 21 (61.7%) a partial response, 8 (23.5%) stable disease, and 4 progressive disease, with a total effective rate of 67.6%. The content of alpha fetoprotein dropped by over 50% in 20 cases (58.8%). The rate of recovery was hepatalgia (88.2%), ascites (47.1%), appetite (55.9%), Performance Status(30.4%). The median follow-up time (MFT) was 281 days (63-558 days), and median progression-free survival was 118.5 days (95%, CI:88.8-148.2days). Adverse reactions (III-IV grade) were not common, with only 4 cases of vomiting and 2 cases of thrombocytopenia (III grade). Conclusions: Lobaplatin-based TACE is an effective and safe treatment for primary liver cancer.
Ji-Chang Zhou,Yu-Mei Zhu,Zheng Chen,Shan He,Shi-jie Zheng,Jun-luan Mo,Xiao-Li Liu,Chun-mei Gong,Bin Hou,Hui Yang 한국유전학회 2015 Genes & Genomics Vol.37 No.5
Two single nucleotide polymorphisms (SNPs), rs2280788 (-28C[G) and rs2107538 (-403G[A), in the promoter region of chemokine (C–C motif) ligand 5 (CCL5) was reported to be involved in the immunoglobulin E (IgE) expression and IgE-mediated allergic reactions. This study was to investigate the characteristics of total serum IgE level, specific allergen sensitivities and the two SNPs in the allergic skin disease (ASD) patients. ASD patients visiting the dermatological outpatient department of a local hospital were included with certain criteria, and the fasting venous blood was sampled for analysis. Total serum IgE was assayed with an ELISA kit, and 14 kinds of allergen-specific IgE were tested with an allergen screening system. The polymerase chain reaction–restriction fragment length polymorphism method was used to analyze the two SNPs. Among the finally included 437 patients aged from 16 to 85 years, 68.2 % was positive for the total serum IgE, 49.2 % was positive for at least one of the assayed allergen-specific IgE, and 35.0 % was sensitive to house dust mite. In the SNPs analysis, the GG/(GA?AA) ratio and G/A ratio for the -403G[A locus in the male and/or female C45 years subgroup were significantly lower in the total serum IgE positive patients than in the negative patients (P\0.05). Weak linkage disequilibrium was found between -403A and -28C alleles in male subgroups adjusted by age. Conclusively, house dust mite was the most common allergen in ASD patients, and -403A allele of CCL5 promoter was a risk factor for IgE-mediated sensitization.
Ying-wu Shi,Kai Lou,Chun Li,Lei Wang,Zhen-yong Zhao,Shuai Zhao,Chang-yan Tian 한국미생물학회 2015 The journal of microbiology Vol.53 No.10
We used Illumina-based 16S rRNA V3 amplicon pyrosequencing to investigate the community structure of soil bacteria from the rhizosphere surrounding Salicornia europaea, and endophytic bacteria living in Salicornia europaea plants and Sueada aralocaspica seeds growing at the Fukang Desert Ecosystem Observation and Experimental Station (FDEOES) in Xinjiang Province, China, using an Illumina genome analyzer. A total of 89.23 M effective sequences of the 16S rRNA gene V3 region were obtained from the two halophyte species. These sequences revealed a number of operational taxonomic units (OTUs) in the halophytes. There were between 22–2,206 OTUs in the halophyte plant sample, at the 3% cutoff level, and a sequencing depth of 30,000 sequences. We identified 25 different phyla, 39 classes and 141 genera from the resulting 134,435 sequences. The most dominant phylum in all the samples was Proteobacteria (41.61%–99.26%; average, 43.30%). The other large phyla were Firmicutes (0%– 7.19%; average, 1.15%), Bacteroidetes (0%–1.64%; average, 0.44%) and Actinobacteria (0%–0.46%; average, 0.24%). This result suggested that the diversity of bacteria is abundant in the rhizosphere soil, while the diversity of bacteria was poor within Salicornia europaea plant samples. To the extent of our knowledge, this study is the first to characterize and compare the endophytic bacteria found within different halophytic plant species roots using PCR-based Illumina pyrosequencing method.