양수 다발성 수지 재접합수의 치험례 : 4 cases report

차진한,박명철,김우경,한근태,김수신,백세민 大韓成形外科學會 1990 Archives of Plastic Surgery Vol.17 No.2

Since the first successful replantation operation, several reports have been recorded replantated finger survival with high success rates. But ten and nine digits amputations are not common. It is even more unusual to have all digits suitable for replantation. 4 cases are presented where replantation of ten and nine digits were done at the department of PRS. Guro Hospital, Korea University from March 1987 to Jun 1988. In these cases, the patients were young and healthy, all of ten and nine digits were sharply amputed near the proximal phalanx, so a replantation team of 8 surgeons in alternating fashion replanted all ten and nine digits over 30 hours and each patient received 28 pints of whole blood during the procedure. Under general anesthesia, replantatin of both started simultaneously. All digits survived completely except partial necosis of right little finger in case 1. The ischemic time of each digit ranged from 4 hours and 45 minutes to 31 hours as shown in table 5. The long ischemic time did not criticaly affected the survival of amputated digit. Follow-up was from 12 months to 20 months postoperatively. The functional evaluation revealed as follows: 1. The grasping power ranged 13 lb to 65 lb(average;39.7lb) 2. The pinching power with index finger ranged 5 lb to 15 lb(average:10.2 lb) 3. The palm to pulp distance ranged 0 cm to 6 cm.(average;2.2cm) 4. The two point discrimination ranged 2.5mm to 22mm.(average;7.8mm) 5. The shape of digits were acceptable aesthetically. 6. All 4 patients were able to use both hands well in all activities of daily living.

흰쥐태아 구개돌기의 융합에 관여하는 E-cadherin과 세포자사에 관한 연구

엄기일,차지훈,김우신,김잉곤,정호삼 大韓成形外科學會 2001 Archives of Plastic Surgery Vol.28 No.5

동종골수이식 후 혈당 및 혈중 지질농도의 변화양상 및 관련인자

이원영,강무일,오은숙,오기원,손현식,윤건호,차봉연,이광우,손호영,강성구,신완식,민우성,김춘주 대한당뇨병학회 2002 Diabetes and Metabolism Journal Vol.24 No.6

연구배경:골수이식은 비교적 젊은 연령의 환자들을 대상으로 하며 면역억제제의 사용기간이 비교적 짧으므로, 주로 고령의 만성 질환 환자에서 시행되는 고형장기이식에서와는 달리 당대사 및 지단백 대사에 있어서 많은 차이점이 있을 것으로 추측된다. 저자들은 전향적 연구를 통하여 골수이식 후 시기별로 혈당 및 혈중지단백 변화 양상을 알아보고 이와 관련된 임상인자들을 규명하고자 하였다. 방법:1998년 10월부터 1999년 8월까지 가톨릭대학교 성모병원에서 동종골수이식을 시행한 환자들 중 43명을 대상으로 이식 전 및 이식 후 1, 2, 3, 4주와 3개월, 6개월에 공복 혈당, 혈중 총 콜레스테롤, 중성지방, 고밀도지단백을 측정하여 골수이식 후 시기별 변화를 관찰하고 여러 임상인자들에 따른 차이를 알아보았다. 결과:1. 공복혈당은 골수이식 수 첫4주 동안 상승하였고 이후 감소하였으나 이식 6개월 시점의 평균 공복혈당은 이식 전보다 유의하게 높게 관찰되었다. 혈중 총 콜레스테롤은 이식 후 1주에 최고치를 형성하였고 이후 기저수준으로 회복되었다가 이식 수 3,6개월에 다시 증가하여 기저치에 비해 유의하게 높았다. 혈중 중성지방은 이식 후 1개월까지 유의하게 증가하였고 이후 감소하여, 3,6개월에는 기저치와 유의한 차이가 관찰되지 않았다. 혈중 고밀도지단백은 이식 후 2,3주에 이식 전보다 유의하게 감소하였고 이후 이식 전 수준으로 회복되었다. 혈중 저밀도지단백은 총 콜레스테롤의 변화와 매우 유사하였다. 2. 골수이식 후 6개월 시점에서 공복 혈당이 126㎎/dL이상인 환자는 7명(16%)이었다. 이들 환자들과 공복혈당 126㎎/dL 미만의 환자 36명을 비교한 결과, 공복혈당이 126㎎/dL 이상인 환자군에서 스테로이드 평균 투여량이 많았고 고밀도지단백이 유의하게 낮았다. 3. 이식편대숙주질환이 발생한 환자는 그렇지 않은 환자보다 이식 후 1, 2, 3주 및 6개월의 총 콜레스테롤이 더 낮았고, 3개월의 공복혈당이 더 높았다. 혈연골수이식 환자군은 비혈연골수이식 환자에 비해 투여된 스테로이드 용량이 더 적음에도 불구하고 이식 후 1, 2, 3주 및 6개월의 총 콜레스테롤 수치가 더 높았다. 스테로이드 고용량 투여군(하루 평균 7.5㎎기준)은 저용량군보다 이식 후 3개월 시점의 공복혈당이 더 높았으나 나머지 시점의 혈당, 혈중 지질농도에 있어서는 유의한 차이가 관찰되지 않았다. 결론:골수이식 후 초기시기에 주로 당 대사 및 지질대사이상이 관찰되며 이는 면역억제제 투여와 관련이 있음을 알 수 있었다. 면역억제제가 고용량 투여되는 합병증 발생 시 이들 대사이상에 관심을 기울여 대처해야 할 것이다. Background: In bone marrow transplantation(BMT), recipients are usually younger and immunosuppressants are open used in shorter period than in solid organ transplantation. Therefore, there might be a difference in glucose and lipid metabolism between BMT and solid organ transplantation. However, the serial changes of metabolic parameters following BMT have not been studied. Therefore, the aim of this study is to investigate the serial changes of blood glucose, lipids and the putative factors that are related with these changes after BMT. Methods: We have prospectively investigated 43 patients who underwent allogeneic BMT. Fasting plasma glucose(FPG), total cholesterol, triglyceride and high-density lipoprotein(HDL) were measured before BMT, and at 1, 2, 3, 4, 12 weeks and 6 months after BMT. The serial changes of these metabolic parameters according to clinical factors including type of BMT, mean daily steroid dosage, and occurrence of graft versus host disease (GVHD) were examined. Results: 1. Mean FPG level increased during 4 weeks after BMT and remained above basal value at post-transplant 6 months. Total Cholesterol level was increased during initial 4 weeks after BMT and was above basal value at post-BMT of 3 and 6 months. Triglyceride level was progressively increased during initial 4 weeks after BMT, but returned to basal value there after. HDL-cholesterol level was significantly decreased during initial 4 weeks after BMT, but returned to basal value there after. 2. Patients with FPG above 126 mg/dL and the other patients, the former received larger amounts of daily steroid and had lower HDL-cholesterol level. 3. The changes of metabolic parameters were different according to type of BMT, steroid dose, and occurrence of GVHD. Conclusion: Although there was increase of FPG, TC, TG and decrease of HDL-C during initial 4 weeks after BMT, these metabolic changes recovered slowly thereafter. Immunosuppressants are thought to be associated with these changes. Further observation will be needed for the long-term effect of BMT on metabolic changes(J Kor Diabetes Asso 24:689~698, 2000).

골수이식 후 골밀도 및 골대사의 변화 : 2년 추적관찰 2-Year Prospective Study

이원영,강무일,오은숙,오기원,한제호,손현식,윤건호,차봉연,이광우,손호영,강성구,신완식,민우성,김춘추 대한내분비학회 2000 Endocrinology and metabolism Vol.15 No.4·5

Primary Skin and Eye Irritation Study of Combined Vaccine (KGCC-95VI) Against Japanese Encephalitis and Hantaan Virus Infection

Shin, Kwang-Soon,Kim, Chul-Joong,Yun, Hyo-In,Park, Jong-Il,Cha, Shin-Woo,Shin, Hyeong-Soon Korean Society of ToxicologyKorea Environmental Mu 1997 Toxicological Research Vol.13 No.1

The primary skin and eye irritancy of the combined vaccine (KGCC-95VI) for the prophylaxis against Japanese encephalitis and Hantaan virus infection recently developed by Korea Green Cross Corporation was investigated. The KGCC-95VI was applied to the back skins of the New Zealand White rabbits. The rabbits were observed for 72 hours and did not exhibit erythema, eschar and edema. The eyes of the rabbits were exposed to the KGCC-95VI. The rabbits were observed for 7 days and did not exhibit any ocular findings on cornea, iris and conjuntivae. The KGCC-95VI is considered not to have the primary skin and eye toxicity in rabbits.

Immunotoxicity of ethyl carbamate in female BALB/c mice : role of esterase and cytochrome P450 esterase와 cytochrome P450의 역할

Cha, Shin Woo,Gu, Hee Kyoung,Lee, Ki Poong,Lee, Mun Han,Han, Sang Seop,Jeong, Tae Cheon 영남대학교 약품개발연구소 2000 영남대학교 약품개발연구소 연구업적집 Vol.10 No.-

Ethyl carbamate, a potent carcinogen, has been characterized to be metabolized by cytochrome P450(P450) and esterase. It has recently been demonstrated that P450 may activate ethyl carbamate to immunotoxic metabolites. To investigate the role of esterase in ethyl carbamate-induced immunosuppression, mice were pretreated intraperitoneally with an esterase inhibitor, diazinon, at 20 ㎎/㎏ 30min prior to the administration of ethyl carbamate intraperi-toneally at 100 and 400㎎/㎏ for 7 consecutive days. Pretreatment with diazinon completely blocked the serum esterase activity. Histopathologically splenic and thymic atrophy was observed when mice were treated with ethyl carbamate, which was potentiated by he pretreatment with diazinon. In spleen, lymphocytes in the periarteriolar lymphoid sheath and the marginal zone appeared to be depleted in the white pulps. In thymus, ethyl carbamate caused a marked depletion of cells in cortex. The antibody response to sheep red blood cells(SRBCs) was more suppressed by ethyl carbamate in diazinon-pretreated groups. These results suggest that the metabolism of ethyl carbamate by esterase may be an inactivation pathway in ethyl carbamate-induced immunosuppression. In addition, ethyl N-hydroxycarbamate, a P450 metabolite, suppressed the lymphoproliferative response induced by lipopolysaccharide and concanavalin A in splenocyte cultures. These results indicate that the metabolism of ethyl carbamate by P450 may be an activation pathway in immunosuppression by ethyl carbamate. ⓒ 2000 Published by Elsevier Science Ireland Ltd. All rights reserved.

General Toxicology Study Using Dogs and Non-human Primates

Cha, Shin-Woo(차신우) 한국실험동물학회 2013 한국실험동물학회 학술발표대회 논문집 Vol.2013 No.8

How to evaluate data and write toxicology report

Shin-Woo Cha(차신우) 한국실험동물학회 2013 한국실험동물학회 학술발표대회 논문집 Vol.2013 No.2

Role of corticosterone in ethyl carbamate-induced immunosuppression in female BALB/c mice

Cha, Shin Woo,Lee, Hu Jang,Cho, Myung Haing,Lee, Mun Han,Koh, Woo Suk,Han, Sang-Seop,Kim, Jung-Ae,Lee, Eung-Seok,Nam, Doo-Hyun,Jeong, Tae Cheon 영남대학교 약품개발연구소 2001 영남대학교 약품개발연구소 연구업적집 Vol.11 No.-

We have recently demonstrated that the antibody response to the T-cell-dependent antigen, sheep red blood cells(SRBCs), was suppressed by ethyl carbamate in female BALB/c mice. At the same doses, ethyl carbamate decreased in the numbers of splenic macrophages. B cells, total T cells, CD4^(+) T cells and CD8^(+) T cells. In addition, the serum level of corticosterone was increased dose-dependently. To investigate the possible role of corticosterone in ethylcarbamate-induced immunosuppression, the antibody response to SRBCs and the subpopulation changes of splenocytes and thymocytes were determined in naive, sham-operated and adrenalectomized (ADX) female BALB/c mice. When the mice were treated intraperitoneally with 400 mg/kg ethyl carbamate, the antibody response was significantly suppressed by ethyl carbamated in naive and sham-operated mice in accompanying the decrease in spleen and thymus weights and/or the increase in the level of serum corticosterone. Meanwhile, the antibody response was not suppressed by ethyl carbamate in the ADX mice. The splenic numbers of total cells, macrophages B and T cells, and CD4^(+) cells were decreased by ethyl carbamate in naive and sham-operated mice. Meanwhile, each cell number was comparable with control in the ADX mice. The flow cytometric analyses on thymocytes did not show obvious differences as seen in the spleen. Finally, when the ADX mice were treated intraperitoneally with 25 mg/kg corticosterone, the antibody response was significantly suppressed. Taken together, our present results suggested that corticosterone might be, at least partially, responsible for ethy; carbamate-induced immunosuppression in female BALB/c mice. ⓒ 2001 Elsevier Science Ireland Ltd. All rights reserved.

Acute Toxicity of Combined Vaccine (KGCC-95VI) Against Japanese Encephalitis and Hantaan Virus Infection

Shin, Kwang-Soon,Kim, Chul-Joong,Yun, Hyo-In,Park, Jong-Il,Cha, Shin-Woo,Shin, Hyeong-Soon Korean Society of ToxicologyKorea Environmental Mu 1997 Toxicological Research Vol.13 No.3

The acute toxicity of the combined vaccine (KGCC-95VI) for the prophylaxis against Japanese encephalitis and Hantaan virus infection. recently developed by Korea Green Cross Corporation, was investigated. KGCC-95VI was administered to the Balb /c mice in two routes, orally and subcutaneously, and into the New Zealand White rabbits subcutaneously. $LD_{50}$ was not accessible as there were no deaths in the group treated even at a dose 800 times the expected clinical dose in both animal species. Between the treated and control groups there were no statistically significant differences in body weight changes and clinical signs during the 14-day observation period, and no pathological gross findings. Accordingly KGCC-95VI is considered not to have the acute toxicity in mice and rabbits.