노화가 인체 중간엽 줄기세포로부터 조골세포로의 증식 및 분화에 미치는 영향

백기현,태현정,오기원,이원영,조정기,권순용,강무일,차봉연,이광우,손호영,강성구,김춘추 대한내분비학회 2003 Endocrinology and metabolism Vol.18 No.3

연구배경: 일반적으로 골다공증과 연관된 위험인자로는 연령, 폐경, 약물, 불충분한 칼슘섭취, 만성질환 및 운동부족 등이 있는데, 특히 노화가 진행할수록 골밀도가 감소하는 것은 잘 알려져 있다. 노화와 관련하여 진행되는 골소실은 조골세포 및 전구조골세포의 기능적 결핍에 의한 골형성의 감소가 주요한 요인으로 여겨지고 있다. 그 동안 연령이 조골모 세포의 양과 조골모 세포로부터 성숙조골세포로의 분화 및 증식에 미치는 영향에 대한 일부 보고들이 있었으나 아직 일치된 견해는 없는 형편이다. 방법: 다양한 연령의 사람으로부터 골수를 채취, 중간엽 줄기세포가 포함된 단핵세포를 분리한 후 조골세포로 분화하기 좋은 조건하에서 배양하였다. 대상군은다시 젊은군과 노령군으로 구분하여 다양한 변수를 비교 분석하였다. 일차배양에서는 CFU-F를 계수하여 골수내 중간엽 줄기세포의 수를 추산하였고, 칼슘측정을 통하여 기질의 무기화 정도를 비교하였다. 계대배양후 이차배양에서는 시기별로 알카리성 포스파타제 활성도를 측정하고 오스테오칼신 mRNA의 발현을 관찰하여 젊은군과 노령군 사이의 증식능 차이를 비교하였다. 또한 이차배양 시기별로 MTT 측정을 하여 양군간에 증식능 차이가 있는지 알아보았다. 결과: 1. 일차배양 15일째에 평균 CFU-F의 수는 젊은군에서 유의하게 많았다(젊은군 148.3±28.9, 노령군 54.3±9.1, p=0.02). CFU-F의 평균면적은 젊은군에서 넓은 경향을 보였으나 통계적으로 유의하지는 않았다. 2. 일차배양 17일 경과 후 양군간에 기질 칼슘 침착정도는 유의한 차이를 보이지 않았다(젊은군 103.6±50.6, 노령군: 114.0±56.5, p=NS). 3. 이차배양 10일째에 젊은군에서 알카리성 포스파타제 활성도가 고령군에 비해 유의하게 높았다(젊은군: 935.5±115.0 U/mg, 노령군: 578.4±115.7U/mg,p.0.05). 고령군에서는 시간 경과에 따른 변화가 미약했으며 전반적으로 알카리성 포스파타제의 활성도가 젊은군에 비해 낮았다. 4. 이차배양도중 오스테오칼신 mRNA의 발현은 배양시기별로 젊은군에 비해 고령군에서 더 낮은 경향을 관찰할 수 있었다. 5. 이차배양 10일과 15일에 젊은군에서 노령군보다세포증식이 유의하게 증가된 양상을 보였다(10앓 젊은군 0.73±0.05, 노령군 0.58±0.04, p=0.05, 15일; 젊은군 0.80±0.05, 노령군 0.70±0.03, p=0.05).결론: 이상의 연구에서 저자들은 노령군에서 젊은군보다 골수 내 중간엽줄기세포의 수가 적고, 노령군에서 유래한 전구조골세포의 성숙조골세포로의 증식 및 분화가 젊은군 보다 감소해 있는 것을 관찰할 수 있었다. Background: Osteoblasts originate from osteoprogenitor cells in bone marrow stroma, termed mesenchymal stem cells (MSCs) or bone marrow stromal cells. Each MSC forms colonies (colony forming units-fibroblasts [CFL-Fs]) when cultured ex vivo. There are some reports about the age-related changes of the number and osteogenic potential of osteoprogenitor cells, but any relationship has not been clearly established in humans. In this study, we counted MSCs using CFU-Fs count and examined the proliferative capacity and differentiation potential of osteoprogenitor cells. Finally, we analyzed how these parameters varied with donor age. Methods: Bone marrow was obtained from the iliac crest of young (n=6, 27.2±8.6 years old) and old (n= 10, 57.4k6.7 years old) healthy donors. Mononuclear cells, including MSCs, were isolated and cultured in osteogenic medium. In primary culture, we compared the colony-forming efficiency of MSCs between the two groups and determined the matrix calcification. When primary culture showed near confluence, the cells were subcultured. Alkaline phosphatase activity, osteocalcin expression by RT-PCR and proliferative potential by MTT assay were examined by the time course of secondary culture. Results: At the 15th day of primary culture, the mean number of CFU-Fs was significantly higher in the younger donors (young: 148.3±28.9, old: 54.3±9.1, p=0.02) and the mean size of CFL-Fs was also larger in the younger donors than the older donors. However, matrix calcification was not different between the two groups (young: 103.6±50.6, old: 114.0±56.5, p=NS). In secondary culture, alkaline phosphatase activities were significantly lower in the older donors. The younger donors showed peak alkaline phosphatase activity at day 10, while the older donors didn't showed a remarkable peak (young: 935.5±115.OU/mg, old: 578.4±115.7U/mg, p<0.05). Total cell number as a proliferative index increased progressively during the secondary culture and a significantly greater cell number was noted in the younger donors. Osteocalcin expression was generally upregulated in the younger donors, but this was not statistically significant. Conclusion: Our study shows that the number of osteoprogenitor cells is decreased during aging and that the proliferative capacity and differentiation potential of osteoprogenitor cells seem to be reduced during aging (J Kor SOC Endocrinol 18:296-305, 2003).

Influence of Starvation of Carbon, Nitrogen, and Phosphate in the medium on the carbohydrate metabolite of Panax ginseng adventitious roots

Yu Kee-Won(유기원),Lim Jong-Hoon(임종훈),Kim Sun-Ja(김선자),Sin Kong-Sik(신공식),Paek Kee Yoeup(백기엽),Kim Mee Hee(김미희),Cha Ahn Hee(차안희),Jeong Jae Woon(정제훈),LEE Yong Wook(이용욱),Lee Sang-Jong(이상종),Yun Seung Rho(윤승로) 한국원예학회 1999 원예과학기술지 Vol.17 No.5

Dysphagia Requiring Medical Attention in Parkinson’s Disease: A Korean Population-Based Study

Cha Seungwoo,Chang Won Kee,Cho Hee-Mun,한경도,백남종,권소현,Kim Won-Seok 대한의학회 2023 Journal of Korean medical science Vol.38 No.15

Background: Patients with Parkinson’s disease (PD) experience both motor and non-motor symptoms, including dysphagia. Although PD is closely associated with dysphagia, the prevalence or risk of dysphagia in PD is unclear, especially in Asian countries. Methods: The prevalence of PD and dysphagia with PD in the general population was analyzed using the Korean National Health Insurance Service (NHIS) database. The prevalence per 100,000 persons of PD and dysphagia with PD from 2006 to 2015 was analyzed in the general population aged ≥ 40 years. Patients newly diagnosed with PD between 2010 and 2015 were compared with those without PD. Results: The prevalence of PD and dysphagia in patients with PD increased continuously during the study period and was highest in the ninth decade of life. The percentage of patients with dysphagia in patients with PD increased with age. Patients with PD showed an adjusted hazard ratio of 3.132 (2.955–3.320) for dysphagia compared to those without PD. Conclusion: This nationwide study showed increasing trends in the prevalence of PD and dysphagia among patients with PD in Korea between 2006 and 2015. The risk of dysphagia was three times higher in patients with PD than that in those without PD, highlighting the importance of providing particular attention.

A Case of Sarcoidosis : Systemic sarcoidosis with cutaneous and subcutaneous involvement

Cha, Mi Kyung,Kim, Tae Yoon,Cho, Baik Kee,Houh, Won,Song, Jeong Sup Korean Dermatological Association 1994 Annals of Dermatology Vol.6 No.1

Blood parameter changes in Korean traditional calves and pigs after foot-and-mouth disease vaccination

Cha, Chun-Nam,Park, Eun-Kee,Yoo, Chang-Yeul,Kim, Suk,Yun, Young Won,Lee, Hu-Jang The Korean Society of Veterinary Science 2017 大韓獸醫學會誌 Vol.57 No.1

This study investigated changes in certain blood parameters in calves and pigs after foot-and-mouth disease (FMD) vaccination. In this study, five calves and five pigs were selected from groups of 10 calves and pigs, respectively, and were vaccinated with an FMD vaccine. The remaining animals formed two non-treatment control groups. Blood samples were collected from all animals on the 1st, 3rd, 5th, and 7th days post-vaccination. In the FMD-vaccinated calves and pigs on day 7 post-vaccination, white blood cell counts, blood urea nitrogen levels, and alanine aminotransferase and aspartate aminotransferase activities were higher than those in the respective controls. The present data suggested that the certain hemato-biochemical parameters on cattle and pigs were meaningfully changed between before and after FMD vaccination.

Study on the Elution Behaviors of Molybdate and Tungstate in Both Hydrochloric and Perchloric Acid by Anion Exchange Chromatograpphy

Cha, Ki-Won,Choi, Jae-Sung,Park, Kee-Chae Korean Chemical Society 1981 Bulletin of the Korean Chemical Society Vol.2 No.2

The elution behaviors of molybdate and tungstate through anion exchange column have been investigated in the various concentration of hydrochloric and perchloric acid. A discussion is made to evaluate the existing these equilibrium and constant according to acidity. In both acids of 0.01-2.0 M concentration range, the existing equilibrium for molybdate and its constant calculated at $20^{\circ}C$ is $10^{18.9}$ for $Mo_8O_2^{4-} + 20H^+ {\rightleftharpoons} 8MoO_2^{2+} + 10H_2O$. In the 3-3.5 M hydrochloric acid it is 0.16 for $MoO_2Cl_2 + Cl^- {\rightleftharpoons} MoO_2Cl^{3-}$ and for the case of tungstate, in the both acids of 0.01-1.0 M concentration range $10^{6.6}$ for $W_{12}O_{39}^{6-} + 6H^+ {\rightleftharpoons} 12WO_3{\cdot}3H_2O$. In higher concentration than 1.0 M both acids the following equilibrium seems to be existed. $12WO_3{\cdot}3H_2O {\rightleftharpoons} 12WO_3 + 3H_2O$

Erratum to pulmonary embolism concurrent with lung cancer and central emboli predict mortality in patients with lung cancer and pulmonary embolism

Cha, Seung-Ick,Shin, Kyung-Min,Lim, Jae-Kwang,Yoo, Seung-Soo,Lee, Shin-Yup,Lee, Jaehee,Kim, Chang-Ho,Park, Jae-Yong,Lee, Won-Kee,Jung, Chi-Young AME Publishing Company 2018 Journal of thoracic disease Vol.10 No.3

<i>SERPINE2</i> Polymorphisms and Chronic Obstructive Pulmonary Disease

Cha, Seung Ick,Kang, Hyo-Gyoung,Choi, Jin Eun,Kim, Min Jung,Park, Jaeho,Lee, Won Kee,Kim, Chang Ho,Jung, Tae Hoon,Park, Jae Yong The Korean Academy of Medical Sciences 2009 JOURNAL OF KOREAN MEDICAL SCIENCE Vol.24 No.6

<P>A number of genome-wide linkage analyses have identified the 2q33.3-2q37.2 region as most likely to contain the genes that contribute to the susceptibility to chronic obstructive pulmonary disease (COPD). It was hypothesized that the <I>SERPINE2</I> gene, which is one of the genes located at the 2q33.3-2q37.2 region, may act as a low-penetrance susceptibility gene for COPD. To test this hypothesis, the association of four <I>SERPINE2</I> single nucleotide polymorphisms (SNPs; rs16865421A>G, rs7583463A>C, rs729631C>G, and rs6734100C>G) with the risk of COPD was investigated in a case-control study of 311 COPD patients and 386 controls. The SNP rs16865421 was associated with a significantly decreased risk of COPD in a dominant model for the polymorphic allele (adjusted odds ratio [OR]=0.66, 95% confidence interval [CI]=0.45-0.97, <I>P</I>=0.03). In haplotype analysis, the GACC haplotype carrying the polymorphic allele at the rs16865421 was associated with a significantly decreased risk of COPD when compared to the AACC haplotype (adjusted OR=0.58, 95% CI=0.38-0.89, <I>P</I>=0.01), and this effect was evident in younger individuals (adjusted OR=0.30, 95% CI=0.14-0.64, <I>P</I>=0.002). This study suggests that the <I>SERPINE2</I> gene contributes to the susceptibility to COPD.</P>

Virucidal efficacy of powder disinfectant containing a phosphate compound against porcine epidemic diarrhea virus

Won-Seok Chae,Eun-Kee Park,Chang-Yeul Yoo,Chun-Nam Cha,Lutfiye Tutkun,Song-Ee Son,Suk Kim,Hu-Jang Lee 한국예방수의학회 2016 예방수의학회지 Vol.40 No.4

The coronavirus porcine epidemic diarrhea virus (PEDV) infects the cells lining the small intestine of a pig and, causes porcine epidemic diarrhea (PED). Owing to its highly infectious nature, PEDV has a substantial economic burden, which results in significant morbidity and mortality in piglets. In this study, the virucidal efficacy of a powder disinfectant containing a phosphate compound against PEDV was investigated. Virucidal efficacy was assessed as the infectivity of PEDV toward Vero cells after exposure of the virus to the disinfectant. PEDV was exposed to the disinfectant in the presence of either hard water (HW) or an organic matter suspension (OM). In the HW condition, PEDV was inactivated by 4-fold dilution of the disinfectant. In the presence of OM, the disinfectant showed virucidal activity with a 2-fold dilution. As the disinfectant possessed virucidal activity against PEDV, it should be an effective reagent for limiting the spread of animal viral diseases.

Analysis of AC Breakdown Characteristics of Fly Ash Cenosphere/Epoxy Composite

Cheong-Won Seo,Jung-Hun Kwon,Young-Gwang Cha,Su-Min Park,Kee-Joe Lim 대한전기학회 2016 Journal of Electrical Engineering & Technology Vol.11 No.5

In this study, AC breakdown properties of cenosphere/epoxy composite are investigated. The thermal properties of the composite are also discussed. The cenosphere/epoxy composites were prepared by epoxy resin filled with 0~40 wt% of hollow fly ash particles (cenospheres). The AC breakdown strength of the composites was measured at 25~125℃. The permittivity, glass transition temperature, and coefficient of thermal linear expansion of the composites were also measured. The experimental results of the cenosphere/epoxy composite were compared with those of the silica/epoxy composite which are widely used as insulating materials in power apparatus. The cenosphere/epoxy composite showed the similar or better performance than silica/epoxy composite in terms of breakdown strength, glass transition temperature, and coefficient of thermal expansion.