백서에서 신 허혈성 손상에 미치는 칼슘 길항제의 효과

문철웅,정종훈,박천국,이승일,배학연,장경식,김만우,정춘해,홍순표,이병래,김호중 朝鮮大學校 附設 醫學硏究所 1993 The Medical Journal of Chosun University Vol.18 No.1

Renal ischemia is one of the most common causes of acute renal failure. Four factors related to the pathogenesis of acute renal failure are vasoconstriction, decreased glomerular filtration rate, tubular back leak of filtrate, and intratubular obstruction. The cellular response to renal ischemic insults include decreased content of adenosine trihosphate, lipid peroxidation induced membrane degradation, alteration in cellular pH, and calcium or phospholipase induced mitochondrial dysfunction. Much attention has been given to the role of increased cellular calcium as a pathogenetic contributor to cell injury during ischemia. Author studied the protective effects of calcium antagonists on cellular injury during renal ischemia in rat. To investigate the protective role of these agents, author measured the amount of malondialdehyde(MDA) and the enzyme activities of free radical scarvengers-superoxide dismutase(SOD), catalase and glutathione peroxidase from non-pretreated group and calcium antagonists pretreated groups in control, ischemia and reflow subgroups. The results were summerized as follows: 1) The amount of MDA in non-pretreated group was higher in the reflow compared with the control(<p<0.01). But, in all pretreated groups, there was no statistically difference in the amount of MDA. 2) The SOD activity in non-pretreated group was lower in both the ischemia and the reflow compared with the control (P<0.05). But, in both verapamil and trifluoperazine-pretreated groups, there was no statistically difference in the SOD activity. 3) Both catalase and glutathione peroxidase activities in non-pretreated group were lower in both the ischemia and the reflow compared with the control (P<0.05). But in all pretreated groups, there was no statically difference in both catalase and glutathione peroxidase activities. These results suggest that free radical mediated ischemic injury by renal artery clamp in rat can be protected by intraperitoneal pretreatment with calcium antagonists. As trifluoperazine has a protective effect in renal ischemia, the calcium activated calmodulin dependent enzyme may play a role in renal ischemic injury.

鷄鳴散이 血漿脂質含量 및 心血管系에 미치는 影響

文炳淳,田炳薰,李承武 대한동의병리학회 1997 동의생리병리학회지 Vol.11 No.1

最近 瘀血에 대한 槪念은 血液流通의 不, 臟腑 또는 局部組織의 血液이 停滯瘀結된 것, 各種 原因에 의한 內出血, 外出血, 血液內의 汚穢하고 不潔한 代謝産物의 저류 및 炎症과 肌肉이나 皮膚의 各種 組織의 增殖과 變性 등이 瘀血의 病態와 一致한다고 報告되고 있다. 鷄鳴散은 大黃, 桃仁, 當歸尾로 構成 있으며, 大黃은 止痙癲癎, 結閉止痛, 破血化瘀, 견담사음, 止血降逆, 攻堅化積, 淸熱解毒, 利膽消黃하고, 桃仁은 破血行瘀, 潤燥滑腸하며 當歸尾는 破血行瘀하여 瘀血로 인한 名種 疾患에 應用되고 있다. 鷄鳴散이 血漿脂質含量 및 心血管系에 미치는 影響을 알아보기 위하여 血漿中의 脂質含量을 測定하고 自發性高血壓白鼠의 血壓 및 心搏數變化, 家兎의 耳殼血管灌流量의 變化, norepinephrine으로 誘導한 白鼠의 血管收縮變化, serotonin과 collagen으로 血栓性塞栓症을 誘發시킨 mouse의 死亡率變化, rat의 血小板凝集抑制 및 血漿凝固因子活性을 觀察하였다. 鷄鳴散이 高脂肪食餌로 誘導한 血漿內의 高脂血症 含量을 減少시키고, HDL-cholesterol, 함량을 有意性있게 增加시켰으며, 自發性高血壓白鼠의 血壓을 有意性있게 降下시켰으나 心搏數의 변화는 관찰할 수 없었다. 鷄鳴散은 家兎의 耳殼血管灌流量을 增加시켰으며, norepinephrine으로 誘導한 白鼠의 血管 收縮을 縮和 시켰고, serotonin과 collagen으로 血栓性塞栓症을 誘發시킨 Mouse의 死亡率을 抑制시켰다. 또한 rat의 血小板凝集을 有意性있게 抑制시키고 血漿凝固因子의 活性檢査에서 prothrombin time과 activated partial thromboplastin time 延長시켰으나 有意性은 없었다. 鷄鳴散은 ex vivo assay에서 rat의 纖維素溶解時間을 有意性있게 短縮시켰고, 纖維素溶解面積을 有意性있게 增加시키고, in vitro assay에서 rat의 纖維素溶解時間을 減少시켰다. The present experiment was designed to investigate the effects of Gaemyungsan water extracts on the Serum Cholesterol Levels and Cardiovascular System in the Experimental Animals. Thus, the changes of serum cholesterol levels and blood pressure and heart rate were measured after oral administration. Measurement of Mortality rate was observed for measuring the effect of Gaemyungsan water extract. Gaemyungsan water extract against puimonary thromboembolism induced by collagen the mixture(0.1㎖/10g, 2㎖/㎏) plus serotonin(5㎖/㎏)in mouse. The effect of Gaemyungsan water extract was examined by observing the change of collagen-induced platelet aggregation, coagulation activity, ex vivo and in vitro fibrinolytic activity of euglobulin faction in rats. The results were summarized as followings. 1. Gaemyungsan decreased the serum cholesterol levels in rats. 2. Gaemyungsan dropped the blood pressure in spontaneous hypertensive rat. 3. The drug increased the auricular blood flow in rabbit. 4. The drug relaxed the artery contraction by pretreated norepinephrine in rat. 5. The drug inhibited the death rate of mouse which was led to thromboembo-lism by serotonin and collagen. 6. The drug inhibited the platelet aggregation in rat. 7. The drug prolonged the prothrombin time and activated partial thromboplastin time on the test of plasma coagulation factor activity in rat, but was not valuable. 8. The drug increased the antithrombin activity in rat and the fibrinogen lyses time was reduced and lyses area was increased. 9. Gaemyungsan reduced fibrinogen lyses time of rat in vitro assay. According to the above mentioned results, Gaemyungsan decteased the serum cholesterol levels and increased the blood flow and dropped the blood pressure by the dilation of blood wessel. And the drug presented the antithrombin activity, inhibited the platelet aggregation.

氣血의 相關性에 關한 文獻的 考察

文炳淳,鄭完祐,申善澔 圓光大學校 韓醫學硏究所 1997 원광한의학 Vol.7 No.1

氣와 血은 水穀의 精微로움과 腎中의 精氣로 만들어지며 肺·脾·腎의 正常的인 機能에 依存한다. 氣와 血은 生命活動을 維持시키는 重要한 基本 要素이다. 氣와 血은 各各 固有의 機能이 있다. 氣는 無形으로 陽에 屬하고 活動力이 强하며 溫煦·推動作用이 있고, 血은 無形으로 陰에 屬하고 活動力이 弱하며 濡養·滋潤作用이 있다. 그러므로 氣와 血 사이에는 密接한 關聯이 있다. 만약 氣와 血의 相互關係를 維持하는 機能이 失調하면 "百病乃變化而生"한다. 氣血의 調和가 疾病의 豫防과 治療의 原則이다. 氣血의 相關性을 生理·病理·治療上의 觀點에서 歷代 文獻을 通하여 考察한 結果 다음과 같은 結論을 얻었다. 1. 氣血의 生理的 關係는 相互依存的이며 氣爲血之師하여 生血, 行血, 攝血하는 作用이 있고 血爲氣之母하여 載氣, 化生氣하는 作用이 있다. 2. 氣血의 病理的 關係는 氣病은 血에, 血病은 氣에 相互影響을 미쳐 氣血同病이 나타나며 氣滯血瘀, 氣不攝血, 血隨氣脫, 氣血兩虛의 類型으로 分類할 수 있다. 3. 氣血의 治療上의 關係는 調氣爲上, 調血次之의 治法이 運用되며 氣滯血瘀時에는 理氣行血法, 氣不攝血時에는 益氣攝血法, 血隨氣脫時에는 大補元氣와 補氣固脫法, 氣血兩虛時에는 氣血雙補法을 使用한다. Ki(氣) and Hul(血) are made of food(水穀)' s essence and vital essence(精氣) in the kidney-Ki(腎氣), and they are dependent on normal function of lung, spleen, kidney. They are basic element for maintenance of vital activity. Ki and Hul have characteristic function. Ki is immaterial, is included in Yang(陽), has energetic activity, makes the body to be warm(溫照), and promotes the activity of body(推動). Hul is material, is included in Yin(陰), has a little activity, and has the effects of the nourishing(滯養) and moisturizing(滋潤). Therefore there is close correlation between Ki and Hul. If Ki and Hul lose their fuction which maintains the mutual connection, all diseases take place from it(百病乃變化而生). The harmonies of Ki and Hul are the prevention of disease and the principle of therapy. The results are as follows 1. The Physiological mutual effects of Ki and Hul have two types. One type is that Ki makes Hul flow(氣爲血之師), the other type is that Hul is a mother of Ki(血爲氣之母). The first type has the effects such as promoting generation of blood(生血), promoting circulration of blood(行血) and promoting Hul-control(攝血). The second type has the effects such as Ki-loading(載氣) and Ki-transforming(化生氣). 2. The Pathological mutual effects of Ki and Hul can be classified as stagnancy of Ki and Hul stasis(氣滯血瘀), out of the Hul-control by the lowering of Ki(氣不攝血), bleeding resulting from exhaustion of Ki(血隨氣脫), and deficiency of both Ki and Hul(氣血兩虛). 3. The principle of medical treatment about the disease by both Ki and Hul(氣血同病) has 4-types. In the case of stagnancy of Ki and Hul stasis(氣滯血瘀), we can promote blood circulation to remove Hul stasis(活血祛瘀法). In the case of being out of the Hul-control by the lowering of Ki(氣不攝血), we can invigorate Ki for promoting Hul-control(益氣攝血法). In the case of bleeding resulting from exhaustion of Ki(血隨氣脫), we can use invigorating primodial Ki(大補元氣法) and invigorating Ki for the prevention of weakness(補氣固脫法). In the case of deficiency of both Ki and Hul(氣血兩虛), we can invigorate Ki and Hul(氣血雙補法).

人蔘이 산소유리기로 손상된 척수신경세포의 손상에 미치는 영향

田炳勳,文炳淳,姜益賢 대한동의병리학회 1998 동의생리병리학회지 Vol.12 No.1

산소자유기는 중풍인 뇌졸중을 비롯하여 뇌허혈, 파킨슨씨병과 같은 뇌질환의 병리적 요인으로 밝혀짐에 따라 이의 독성효과나 작용현상에 대한 기전을 규명하기 위하여 많은 연구가 진행되어 왔다. 그러나 산소자유기의 독성효과에 대하여 아직까지 이에 대한 자세한 기전이 밝혀져 있지 않고 치료방법 역시 미흡한 실정이다. 본 연구는 산소자유기의 신경독성 효과에 대한 기전을 규명하기 위하여 배양소의 희소돌기아교세포에 여러 농도의 산소자유기를 처리한 다음 산소자유기의 독성효과를 측정하였으며 또한 산소자유기의 독성효과에 대한 인삼추출물의 영향을 MTT assay법으로 조사하여 다음과 같은 결론을 얻었다. xanthine oxidase(XO)와 hypoxanthine(HX)은 처리한 농도에 비례하여 배양 신경세포의 생존율을 현저하게 감소시켰으며, 인삼추출물을 배양 희소돌기아교세포에 전처리한 결과 XO/HX의 독성효과를 유의성있게 방어하였다. 이상의 결과로부터 산소자유기는 배양 소의 희소돌기아교세포에 독성을 나타냈으며 인삼추출물이 산소자유기의 독성을 방어하는데 효과적인 것으로 나타났다. To elucidate the neurotoxic mechanism of oxygen radicals in cultured bovine oligodendrocytes, cytotoxic effect was measured by MTT assay after cultured oligodendrocytes were grown in the medium containing various concentrations of oxygen radicals. In addition, neuroprotective effect of herb extract, Korean red Ginseng was examined by MTT assay in cultured oligodendrocytes. Cell viability of cultured oligodendrocytes was remarkably decreased by xanthine oxidase(XO) and hypoxanthine(HX) in dose-dependent manner, and Korean red Ginseng protected remarkably oxidant-induced neurotoxicity in these cultures. From the above results, it is suggested that oxygen radicals is toxic in cultured bovine oligodendrocytes, and herb extracts such as Korean red Ginseng are effective in blocking the neurotoxicity induced by oxygen radicals in cultured bovine oligodendrocytes.

病院施設의 長期計劃에 관한 硏究

권순정,문병국 순천향대학교 부설 산업기술연구소 1999 순천향 산업기술연구소논문집 Vol.5 No.2

생물학적 폐수처리시 휴지기간이 처리특성에 미치는 영향에 관한 연구

김상수,문병현,서규태,이택수 7개 국립대학교 환경연구 논문집 공동발행 위원회 2004 공업기술연구 Vol.4 No.-

Under recent situation of more stringent effluent regulations, small scale biological process for industrial wastewater treatment has on operational problems, such as highly variable loadings into the system. Especially, the seafood processing industry has difficulties to treat is because of its highly variable organic loading and high saline concentration (>30g/d㎥). High salt concentrations disrupt metabolic functions and cause plasmolysis and/or loss of activity of microbial flora, hence the biological treatment of the saline wastewaters could produce low COD removal efficiency. On the basis of background mentioned above, an appropriate operation of the existing treatment plant is required for stable effluent discharge at even highly influent loading. In this study, effects of long term ( days) idle periods on floc characteristics were investigated in treatment of saline wastewater by SBR. Temporal stop of feeding affects biological activity in the reactors and then floc structures such as size and fractal dimension. After a certain period of reoperation for orgnic activity, the size and fractal dimension of floc start to increase.

四君子湯 抽出液이 家兎의 體液性 免疫反應에 미치는 影響

林圭庠,文炳淳,李起男 圓光大學校 醫科學硏究所 1987 圓光醫科學 Vol.3 No.1

The present experiment was investigated on the effects of Sagunjatang extract on the humoral immune response of rabbit. In order to understand the immune respone of the extract, one of the experimental groups was treated with the antigen, E. coli Ry 13 suspension and the extract. And the serum separated from the treated group was analyzed. The results obtained were as follows : 1. The body weight of the rabbit treated with Sagunjatang extract was increased remarkably. 2. Sagunjatang extract did not affect the number of erythrocytes, however, it increased the number of WBC in small amount. It means that the extract activates the WBC related with producing antibody. 3. The synthesis of γ-globulin was stimulated by the Sagunjatang extract in the present experiment.

交感丹 煎陽液이 心臟機能에 미치는 影響

張鎬鉉,文炳淳,金世吉 圓光大學校 韓醫學硏究所 1992 원광한의학 Vol.2 No.1

The present experiments were designed to investigate the effects of Kyogamdan(交感丹) water extract on the Heart Functions. The inhibitory effects of Kyogamdan on the contractility of cardiac muscle and ATPase activities were analyzed with Physiogram and enzyme Kinetics. 1. Kyogamdan water extract decreased the contractility of ventricular muscle with the increased concentration of Kyogamdan extract. 2. The (Mg^++ -Ca^++) -ATPase activity decreased with the increased concentration of Kyogamdan EXTRACT, AND THE VALUES OF Ki and Km were 0.1 and 2.2 respectively. 3. The cerebral cell membrane(Na^+ -K^+)-ATPase activity increased with the increased concentration of Kyogamdan extract. 4. Verapamil (calcium antagonist )also inhibits the (Mg^++ -Ca^++)-ATPase and thus Kyogamdan was suggested to act as a blocking agent on the calcium active transport. 5. Electric Shock induced the abnormal secretion of AST, CK and LDH, however, the oral administration of Kyogamdan extract recovered the abnormal release of the enzymes. From the above results, it was suggested that the effects of Kyogamdan on the heart act as a stabilizer against stress.

溫膽湯 煎湯液이 家兎의 心血管系에 미치는 影響

成彊慶,文炳淳,朴暎淳,金世吉 圓光大學校 韓醫學硏究所 1994 원광한의학 Vol.4 No.1

The present experiments were designed to investigate the effects of Ondamtang water extract(Ondamtang) on the blood circulation, bile acid secretion and mental tension in normal and hyperlipidemia induced rabbits. In order to control the precise chemical and physical conditions, the experimental rabbits were supplied with calorie limited food, and the hyperlipidemia rabbits were induced by oral administration of cholesterol(500㎎/㎏) for 3 weeks. The results obtained were summerized as follows: 1. Ondamtang increased the bile secretion and serum bile acid level in rabbits. 2. Bile solution decreased the blood pressure after injection through the ear vien in rabbits. 3. The blood pressure was decreased after injecting Ondamtang through the ear vein. And it was also decreased after oral administration of Ondamtang for 5 days. 4. The decrease of blood pressure was not dependant on the autonomic nervous system. 5. Ondamtang activated the Mg^2+ -Ca^2+ -ATPase activity of cardiac indoplasmic reticulum, and thus inhibited the cardiac muscle contraction. 6. Ondamtang stimulated the dilatation of the peripheral blood vessel. 7. Ondamtang decreased the serum creatinine level by stimulating the renal filtration and thus it increased the kidney function in hypelipidemia rabbits. 8. Ondamtang decreased the arteriosclerosis index by effecting the metabolism of cholesterol and lipid in hypdrlipidemia induced rabbits. 9. Ondamtang inhibited the Na^+ -K^+ -ATPase activity of the cerebral cortex, and decreased the sensitivity of the electrical shock. According to the above results, Ondamtang improved blood circulation by dilatation the capillary lumen and decreasing in blood viscosity, promoted bile acid secretion, inhibited the nerve transmission, and relieved the mental tension.

O_3와 O_3/UV를 이용한 수용액중의 음이온계면활성제의 처리에 관한 연구

김태성,문병현,이택순,서규태 7개 국립대학교 환경연구 논문집 공동발행 위원회 2001 환경연구논문집 Vol.1 No.1

Water pollution by discharging surfactant into receiving water has become serious. One of pollutants widely contained in domestic wastewater is surfactants. To remove these surfactants, ozonation has been used but it doesn't perfectly remove relatively non-degradable organic matter. Recently, an advanced oxidation process using OH radical has been developed and applied for removing such non-degradable pollutants. In this study it was treated Sodium Dodecyl Benzene Sulfonate (DBS) using Ozone and Ozone/UV process. The results showed that removal efficiencies by Ozonation increased as the dissolved ozone concentration, initial temperature and pH increased. The combination process of Ozone/UV for DBS removal resulted in 4 times higher reaction rate than the Ozonation process. The combination process showed higher removal efficiencies for treating laundry wastewater and the removal efficiencies was increased by pretreating particle matters.