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아토피피부염에서 포도상구균 집락과 장독소 유무에 따른 임상양상
반혜련 ( Hye Ryun Ban ),이소연 ( So Yeon Lee ),최준기 ( Jun Gi Choi ),김효빈 ( Hyo Bin Kim ),송영화 ( Young Wha Song ),김병주 ( Byoung Joo Kim ),유진호 ( Jin Ho Yu ),김미나 ( Mi Na Kim ),홍수종 ( Soo-Jong Hong ) 대한천식알레르기학회 2008 천식 및 알레르기 Vol.28 No.2
Background: Atopic dermatitis (AD) is a chronically relapsing allergic skin inflammatory disease with a high incidence in the early childhood. Staphylococcus aureus colonization (S. aureus) is frequent in the skin of patients with AD and is one of the important environmental factors that develop or exacerbate this disorder. Objective: The aim of this study was to investigate the different clinical features of AD with S. aureus colonization and to seek its relation with the clinical features of AD. Method: We recruited 342 children with AD who visited the pediatric allergy clinic in Asan Medical Center from July 2003 to January 2007. They were divided into 2 groups based on the results of skin cultures: group 1 with no bacteria cultured and group 2 with S. aureus cultured. The clinical and laboratory data, such as SCORAD (SCORing Atopic Dermatitis), total eosinophil count (TEC), eosinophil fraction (%), serum total IgE, serum eosinophil cationic protein (ECP) and staphylococcal enterotoxin A and B -specific IgE levels, were evaluated and were compared between individual groups. Result: There was a statistical difference between groups 1 and 2 in TEC, eosinophil fraction (%), total IgE, serum ECP and SCORAD index. There was a significant difference in total IgE between the enterotoxin-negative and enterotoxin-positive groups. S. aureus colony counts were significantly correlated with total eosinophil count (r=0.259, P= 0.003), eosinophil (%) (r=0.196, P=0.009), and serum ECP (r=0.212, P=0.035). Total IgE levels were significantly correlated with specific IgE levels to enterotoxins A and B (r=0.335, P=0.012; r=0.393, P=0.002). S. aureus colonization and specific IgE levels to staphyloccal enterotoxins A were correlated with SCORAD index and total IgE levels. Conclusion: Skin infections with S. aureus may be associated with pathogenesis of AD. (Korean J Asthma Allergy Clin Immunol 2008;28:105-112)
Kim, Young-Jon,Kim, Byoung-Ryun,Ryu, Jae-Suk,Lee, Gyeong-Ok,Kim, Hak-Ryul,Choi, Keum-Ha,Ryu, Jae-Won,Na, Kyoung-Suk,Park, Min-Cheol,So, Hong-Seob,Cho, Ji-Hyun,Park, Do-Sim Blackwell Scientific Publications 2017 International journal of gynecological cancer Vol.27 No.2
<B>Objective</B><P>Heterogeneous nuclear ribonucleoprotein A1 (HNRNPA1), serine/arginine-rich splicing factor 1 (SRSF1), and SRSF3 are splicing regulators associated with oncogenesis. However, the alterations of SF proteins and their diagnostic values in cervical cancer are unclear. To apply SFs clinically, effective marker selection and characterization of the target organ properties are essential.</P><B>Materials and Methods</B><P>We concurrently analyzed HNRNPA1, SRSF1, SRSF3, and the conventional tumor markers squamous cell carcinoma antigen (SCCA) and carcinoembryonic antigen (CEA) in cervical tissue samples (n = 127) using semiquantitative immunoblotting. In addition, we compared them with p16 (cyclin-dependent kinase inhibitor 2A [CDKN2A]), which has shown high diagnostic efficacy in immunohistochemical staining studies and has been proposed as a candidate protein for point-of-care screening biochemical tests of cervical neoplasia.</P><B>Results</B><P>HNRNPA1, higher molecular weight forms of SRSF1 (SRSF1-HMws), SRSF3, CEA, and p16 levels were higher (<I>P</I> < 0.05) in cervical carcinoma tissue samples than in nontumoral cervical tissue samples. However, the levels of SRSF1-Total (sum of SRSF1-HMws and a lower molecular weight form of SRSF1) and SCCA, a commonly used cervical tumor marker, were not different between carcinoma and nontumoral tissue samples. In paired sample comparisons, HNRNPA1 (94%) showed the highest incidence of up-regulation (carcinoma/nontumor, >1.5) in cervical carcinoma, followed by p16 (84%), SRSF1-HMws (69%), SRSF3 (66%), CEA (66 %), SCCA (32%), and SRSF1-Total (31%). HNRNPA1 (92%) and p16 (91%) presented the two highest diagnostic accuracies for cervical carcinoma, which were superior to those of SRSF3 (75%), SRSF1-HMws (72%), CEA (72%), SCCA (59%), and SRSF1-Total (55%).</P><B>Conclusions</B><P>Our results identified that HNRNPA1 is the best diagnostic marker among the SFs and conventional markers given its excellent diagnostic efficacy for cervical carcinoma, and it has a p16-comparable diagnostic value. We suggest that HNRNPA1 is an additional effective target protein for developing cervical cancer detection tools.</P>
Lung Microbiome Analysis in Steroid-Naïve Asthma Patients by Using Whole Sputum
Jung, Jae-Woo,Choi, Jae-Chol,Shin, Jong-Wook,Kim, Jae-Yeol,Park, In-Won,Choi, Byoung Whui,Park, Heung-Woo,Cho, Sang-Heon,Kim, Kijeong,Kang, Hye-Ryun The Korean Academy of Tuberculosis and Respiratory 2016 Tuberculosis and Respiratory Diseases Vol.79 No.3
Background: Although recent metagenomic approaches have characterized the distinguished microbial compositions in airways of asthmatics, these results did not reach a consensus due to the small sample size, non-standardization of specimens and medication status. We conducted a metagenomics approach by using terminal restriction fragment length polymorphism (T-RFLP) analysis of the induced whole sputum representing both the cellular and fluid phases in a relative large number of steroid $na{\ddot{i}}ve$ asthmatics. Methods: Induced whole sputum samples obtained from 36 healthy subjects and 89 steroid-$na{\ddot{i}}ve$ asthma patients were analyzed through T-RFLP analysis. Results: In contrast to previous reports about microbiota in the asthmatic airways, the diversity of microbial composition was not significantly different between the controls and asthma patients (p=0.937). In an analysis of similarities, the global R-value showed a statistically significant difference but a very low separation (0.148, p=0.002). The dissimilarity in the bacterial communities between groups was 28.74%, and operational taxonomic units (OTUs) contributing to this difference were as follows: OTU 789 (Lachnospiraceae), 517 (Comamonadaceae, Acetobacteraceae, and Chloroplast), 633 (Prevotella), 645 (Actinobacteria and Propionibacterium acnes), 607 (Lactobacillus buchneri, Lactobacillus otakiensis, Lactobacillus sunkii, and Rhodobacteraceae), and 661 (Acinetobacter, Pseudomonas, and Leptotrichiaceae), and they were significantly more prevalent in the sputum of asthma patients than in the sputum of the controls. Conclusion: Before starting anti-asthmatic treatment, the microbiota in the whole sputum of patients with asthma showed a marginal difference from the microbiota in the whole sputum of the controls.
Lim, Sun Min,Choi, Jae Woo,Hong, Min Hee,Jung, Dongmin,Lee, Chang Young,Park, Seong Yong,Shim, Hyo Sup,Sheen, Seungsoo,Kwak, Kyeong Im,Kang, Dae Ryong,Cho, Byoung Chul,Kim, Hye Ryun Elsevier 2019 Lung cancer Vol.131 No.-
<P><B>Abstract</B></P> <P><B>Objectives</B></P> <P>Radon, a natural radiation, is the leading environmental cause of lung cancer in never-smokers. However, the radon exposure impact on the mutational landscape and tumor mutation burden (TMB) of lung cancer in never-smokers has not been explored. The aim of this study was to investigate the mutational landscape of lung adenocarcinoma in never-smokers who were exposed to various degrees of residential radon.</P> <P><B>Materials and methods</B></P> <P>To investigate the effect of indoor radon exposure, we estimated the cumulative exposure to indoor radon in each house of patients with lung cancer with a never-smoking history. Patients with at least 2 year-duration of residence before the diagnosis of lung adenocarcinoma were included. Patients were subgrouped based on the median radon exposure level (48 Bq/m<SUP>3</SUP>): radon-high <I>vs.</I> radon-low and targeted sequencing of tumor and matched blood were performed in all patients.</P> <P><B>Results</B></P> <P>Among 41 patients with lung adenocarcinoma, the TMB was significantly higher in the radon-high group than it was in the radon-low group (mean 4.94 <I>vs</I>. 2.6 mutations/Mb, <I>P</I> = 0.01). The recurrence rates between radon-high and radon-low group did not differ significantly. Mutational signatures of radon-high tumors showed features associated with inactivity of the base excision repair and DNA replication machineries. The analysis of tumor evolutionary trajectories also suggested a series of mutagenesis induced by radon exposure. In addition, radon-high tumors revealed a significant protein-protein interaction of genes involved in DNA damage and repair (<I>P</I> < 0.001).</P> <P><B>Conclusions</B></P> <P>Indoor radon exposure increased the TMB in never-smoker patients with lung adenocarcinoma and their mutational signature was associated with defective DNA mismatch repair.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Radon is the leading environmental cause of lung cancer in never-smokers. </LI> <LI> We investigated the mutational landscape in never-smokers who were exposed to residential radon. </LI> <LI> Indoor radon exposure increased tumor mutation burden. </LI> </UL> </P>
Role of p38 MAPK on the Down-Regulation of Matrix Metalloproteinase-9 Expression in Rat Astrocytes
Shin, Chan-Young,Lee, Woo-Jong,Choi, Ji-Woong,Choi, Min-Sik,Park, Gyu-Hwan,Yoo, Byoung-Kwon,Han, Sun-Young,Ryu, Jae-Ryun,Choi, Eui-Yul,Ko, Kwang-Ho 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.5
In spite of their pathophysiological importance in neuro-inflammatory diseases, little is known about the signal transduction pathways that lead to the induction of matrix metalloproteinases(MMPs) in the central nervous system. We reported previously that lipopolysaccharide (LPS) induced MMP-9 expression through ERK1/2 pathway in rat primary astrocytes (Glia 41:15-24, 2003). Here, we investigated the role of other MAPK pathways, including p38 and JNK/SAPK, on the regulation of MMP-9 expression in LPS-stimulated rat primary astrocytes. LPS activated both p38 and JNK in astrocytes. Treatment with a specific p38 MAPK inhibitor SB203580, but not JNK inhibitor Sp600f 25, increased the LPS-stimulated MMP-9 expression in a concentration-dependent manner. Anti-inflammatory cytokines, including IFN-${\gamma}$ and 1L-4,activated p38 MAPK and decreased MMP-9 production in LPS-stimulated astrocytes. When p38 MAPK activation was blocked by SB203580, the inhibitory effects of these cytokines on MMP-9 induction were abolished. Finally, direct injection of SB203580 into the lateral ventricle of rat brain increased the LPS-induced MMP-9 activity in cerebral cortex. Altogether, these results suggest that p38 activation down-regulates the inflammatory stimulation-induced over-expression of MM P-9, both in primary astrocytes and in cerebral cortex. The elaborate inter-play between ERK1/2 and p38 pathways provides a more sophisticated mechanism for regulating MMP-9 activity in neuroirflammatory diseases.
Lung Microbiome Analysis in Steroid-Naive Asthma Patients by Using Whole Sputum
( Jae Woo Jung ),( Jae Chol Choi ),( Jong Wook Shin ),( Jae Yeol Kim ),( In Won Park ),( Byoung Whui Choi ),( Heung Woo Park ),( Sang Heon Cho ),( Kijeong Kim ),( Hye Ryun Kang ) 대한결핵 및 호흡기학회 2016 Tuberculosis and Respiratory Diseases Vol.79 No.3
Background: Although recent metagenomic approaches have characterized the distinguished microbial compositions in airways of asthmatics, these results did not reach a consensus due to the small sample size, non-standardization of specimens and medication status. We conducted a metagenomics approach by using terminal restriction fragment length polymorphism (T-RFLP) analysis of the induced whole sputum representing both the cellular and fluid phases in a relative large number of steroid naive asthmatics. Methods: Induced whole sputum samples obtained from 36 healthy subjects and 89 steroid-naive asthma patients were analyzed through T-RFLP analysis. Results: In contrast to previous reports about microbiota in the asthmatic airways, the diversity of microbial composition was not significantly different between the controls and asthma patients (p=0.937). In an analysis of similarities, the global R-value showed a statistically significant difference but a very low separation (0.148, p=0.002). The dissimilarity in the bacterial communities between groups was 28.74%, and operational taxonomic units (OTUs) contributing to this difference were as follows: OTU 789 (Lachnospiraceae ), 517 (Comamonadaceae, Acetobacteraceae , and Chloroplast ), 633 (Prevotella ), 645 (Actinobacteria and Propionibacterium acnes ), 607 (Lactobacillus buchneri, Lactobacillus otakiensis, Lactobacillus sunkii , and Rhodobacteraceae ), and 661 (Acinetobacter, Pseudomonas , and Leptotrichiaceae ), and they were significantly more prevalent in the sputum of asthma patients than in the sputum of the controls. Conclusion: Before starting anti-asthmatic treatment, the microbiota in the whole sputum of patients with asthma showed a marginal difference from the microbiota in the whole sputum of the controls.