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Bich Ngoc Vu,Jae Yong Yoo,Ki Seong Ko,Young Eun Lee,Ha Na Choi,Yoo Na Lee,Mi Hui Jang,Kyun Oh Lee 한국당과학회 2022 한국당과학회 학술대회 Vol.2022 No.07
To cope with multiple stresses caused by various biotic and abiotic stimuli and consequently increased accumulation of misfolded proteins, eukaryotic cells activate a series of adaptive mechanisms that are known as the unfolded protein response (UPR). In response to cellular stresses, phosphorylation of the α subunit of eukaryotic initiation factor 2 (eIF2α) attenuates general protein synthesis. However, it also favors the selective translation of mRNAs with upstream open reading frames (uORFs) such as ATF4, a key regulator of the integrated stress response (ISR), in mammalian cells. The ISR is an evolutionarily conserved translational and transcriptional program initiated upon phosphorylation of eIF2α. However, no studies to date have been conducted on the ISR mediated by phosphorylation of eIF2α, and key regulators responsible for the ISR have not been identified in plants. In this study, we performed in silico analysis to investigate functional orthologs of the ATF4, and confirmed that some genes belong to bZIP family containing uORFs in the 5′UTR are potential candidates. However, it is not well understood whether they are involved in plant ISR mediated by phosphorylation of eIF2α. During prolonged ER stress, transcription of these genes increased by 2-3 fold. Arabidopsis plants overexpressing showed a more sensitive phenotype than Col-0 and knockout mutant plants under tunicamycin-induced ER stress condition, while plants overexpressing with uORF did not show any difference. In addition, the expression level of bZIP GFP fusion protein without uORF is increased under ER stress conditions compare to normal conditions. These results suggest that uORF-containing bZIP genes may have a role in plant ISR. * Corresponding
Production of an antibody for treatment of rheumatoid arthritis using plants
Ha Na Choi,Ki Seong Ko,Jae Yong Yoo,Bich Ngoc Vu,Young Eun Lee,Yoo Na Lee,Mi Hui Jang,Kyun Oh Lee 한국당과학회 2022 한국당과학회 학술대회 Vol.2022 No.07
Rheumatoid arthritis, which is known as an autoimmune phenomenon as the main mechanism, is a chronic inflammatory disease that causes inflammation in several joints, such as the hands, wrists, feet, and ankles. Humira, a monoclonal antibody, recognizes tumor necrosis factor-alpha (TNF-α) and is used to treat rheumatoid arthritis. Humira is mostly produced in animal cell-based production systems that have high investment costs, high production costs, and risk of contamination. In this study, Humira was produced using plants to overcome the problems of the existing production system. In order to improve the efficacy of the antibody and reduce side effects, glycoengineered plants without plant-specific residues developed in previous studies were used. In addition, in order to solve the low expression level of the recombinant protein, a vector containing the antibody gene was constructed using a virus-based expression system in which expression is regulated by an inducible promoter. The expression vector was introduced into Agrobacterium to transform wild-type plants and glycoengineered plants, and the transformed plants were selected using an antibiotic-containing medium and a reporter gene. The expression of the antibody expressed in the plant was confirmed using an antibody specific to the protein extracted from the leaf. It was identified at a size of approximately 50 kDa and 27 kDa, respectively, representing the heavy and light chains of the antibody. Single copy plants were selected through the segregation test, and homozygous lines were established through the selection process. The expression level of the recombinant protein by the inducible promoter was compared according to the concentration and time of the inducer. These results show the potential for lower production costs and safer production of rheumatoid arthritis drugs using plants.
Production of an enzyme for treatment of Gaucher's disease using plants
Yoo Na Lee,Ki Seong Ko,Jae Yong Yoo,Bich Ngoc Vu,Young Eun Lee,Ha Na Choi,Mi Hui Jang,Kyun Oh Lee 한국당과학회 2022 한국당과학회 학술대회 Vol.2022 No.07
Gaucher's disease is a metabolic disorder in which a functional deficiency of an enzyme occurs due to a mutation in the gene encoding glucocerebrosidase (GC), resulting in multiple organ malfunctions. Gaucher's disease is being treated with enzyme replacement therapy, which compensates for the enzyme deficiency by administering activated GCs produced in mammalian cells. However, there is a potential of contamination by pathogenic viruses or prions in the mammalian cell-based manufacturing method, as well as a high production cost. In this study, GCs were expressed in plants that produce customized N-glycans to overcome the limitations of the mammalian cell-based production system and to produce a safe treatment drug for Gaucher's disease. A human cDNA library was used to clone a region expressing a GC gene, and sequence analysis was performed. In addition, a binary vector was created and introduced into plants using Agrobacterium to insert the sequence-confirmed GC gene. Plants into which the GC gene was introduced were selected using an antibiotic-containing media, and the presence or absence of the GC gene introduction into the transformed plants was confirmed using polymerase chain reaction (PCR). Furthermore, proteins were collected from the plant leaves into which the gene was inserted, and GC expression levels in the transformed plants were measured using a GC antibody. Finally, a single cloned plant was selected through the antibiotic-resistant isolate ratio. These findings suggest that employing plants, it is possible to overcome the disadvantages of the present mammalian cell-based manufacturing method, decreasing the production cost of pricey Gaucher's disease therapy, and effectively generating safer enzyme treatment.
장빛나 ( Bich Na Jang ),주재홍 ( Jae Hong Joo ),김휘준 ( Hwi Jun Kim ),박은철 ( Eun-cheol Park ),장성인 ( Sung In Jang ) 한국보건행정학회 2021 보건행정학회지 Vol.31 No.2
Unmet healthcare is an important indicator to measure accessibility of healthcare services. To examine the latest status of unmet healthcare needs in South Korea, the four different data which is composed of nationally representative sample of South Korean population were used; the Korea Health and Nutrition Examination Survey (KNAHANES, 2007-2019), the Community Health Survey (CHS, 2008-2019), the Korea Health Panel Survey (KHP, 2011-2017), and the Korean Welfare Panel Study (KOWEPS, 2006-2019). The proportion of individuals reporting unmet healthcare needs were 5.8% (KNHANES), 5.3% (CHS), and 11.6% (KHP). Annual percentage change (APC) which identifies trend for the follow-up period was -9.5%, -8.0%, and -6.5%, respectively. The proportion of individuals reporting unmet healthcare needs due to cost were 1.1% (KNAHANES), 0.7% (CHS), 2.4% (KHP), and 0.4% (KOWEPS). The APC was -10.5%, -14.2%, -12.2%, and -19.6%, respectively. Compared to last year, the rate of unmet healthcare needs has declined in general. However, the low-income and the elderly population were reporting the highest rate of unmet health care needs, and the disparity between lowest and highest groups were remained. These results suggest that adequate benefit coverage is needed for low-income and elderly population.
패시브주택 에너지 절감목표 수준별 요소기술 통합구성에 관한 연구
김빛나(Kim Bich-na),윤종호(Yoon Jong-Ho),신우철(Shin U-Cheul),백남춘(Baek Nam-Choon) 한국태양에너지학회 2010 한국태양에너지학회 학술대회논문집 Vol.2010 No.11
It is essential to reduce energy consumption in Residential Buildings from 2012 because government strengthened the standard of annual total energy consumption by stages. So, this study analyzed energy saving rate of passive house by stages. it suggests the application of necessary technical elements to satisfy with the level in progressive energy performance objectives. The level of progressive energy saving rate of passive house is 30%, 40%, 50%. According to analyze the elemental heat, It can know that the heat loss from window per unit area loss is large. Passive technical elements is the insulation of wall and roof, highly efficient window system, reduction of infiltration. It is analyzed energy saving rate of base house by simulating with building energy analysis program. Consequently energy saving rate by the insulation of wall is the most largest. It is analyzed energy saving rate of base house by combining technical elements to apply base house. Energy saving proposal according to energy saving rate is calculated at 240 unit. Energy saving rate show from minimum 22.2% to maximum 51%. It selected the proposal of passive house of energy saving rate 30%, 40%, 50% based aim of this study from among these. Result is arrived that 30% saving proposal is 7 unit, 40% saving proposal is 8 unit and 50% saving proposal is 2 unit. It is expected to apply data of standard of annual total energy consumption from 2012 as this study suggests integrated application to compose technical elements of passive house by the level of energy saving.
해방이후 한국 현대건축가 교육환경 및 작품유형 특성에 관한 연구
김빛나(Kim Biche-Na),김승제(Kim Seung-Je) 대한건축학회 2008 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.28 No.1(계획계)
The porpose of this study was analysis fix study subject in 185 architect award to Korean Institute of Architects, Architectural Institute of Korea, Korean Institute of education facilities and analysis Architect education environment, career type's flowing analysis, architecture type. Learning lineage, career and quality of work to Korea architect learn Modem Architecture education suggest to flowing and special quality according objective data analysis