A review of the innovative gas separation membrane bioreactor with mechanisms for integrated production and purification of biohydrogen

Bakonyi, Pé,ter,Kumar, Gopalakrishnan,Bé,lafi-Bakó,, Katalin,Kim, Sang-Hyoun,Koter, Stanislaw,Kujawski, Wojciech,Nemestó,thy, Ná,ndor,Peter, Jakub,Pientka, Zbynek Elsevier 2018 Bioresource technology Vol.270 No.-

<P><B>Abstract</B></P> <P>This review article focuses on an assessment of the innovative Gas Separation Membrane Bioreactor (GS-MBR), which is an emerging technology because of its potential for in-situ biohydrogen production and separation. The GS-MBR, as a special membrane bioreactor, enriches CO<SUB>2</SUB> directly from the headspace of the anaerobic H<SUB>2</SUB> fermentation process. CO<SUB>2</SUB> can be fed as a substrate to auxiliary photo-bioreactors to grow microalgae as a promising raw material for biocatalyzed, dark fermentative H<SUB>2</SUB>-evolution. Overall, these features make the GS-MBR worthy of study. To the best of the authors’ knowledge, the GS-MBR has not been studied in detail to date; hence, a comprehensive review of this topic will be useful to the scientific community.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A novel integrative system has been proposed for biohydrogen technology. </LI> <LI> Innovative Gas Separation Membrane Bioreactors are evaluated. </LI> <LI> Simultaneous biohydrogen production and separation is outlined. </LI> <LI> Gas separation membrane technology for CO<SUB>2</SUB> removal is suggested. </LI> <LI> Algae cultivation using the CO<SUB>2</SUB> removed and biohydrogen effluent is assessed. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Architectural engineering of bioelectrochemical systems from the perspective of polymeric membrane separators: A comprehensive update on recent progress and future prospects

Bakonyi, Pé,ter,Koó,k, Lá,szló,Kumar, Gopalakrishnan,Tó,th, Gá,bor,Ró,zsenberszki, Tamá,s,Nguyen, Dinh Duc,Chang, Soon Woong,Zhen, Guangyin,Bé,laf Elsevier 2018 Journal of membrane science Vol.564 No.-

<P><B>Abstract</B></P> <P>Significant advances in the design of bioelectrochemical systems (BES) have promoted these applications to be seen as contemporary biotechnological platforms. However, notable issues in system architecture are still to be addressed and overcome, in particular concerning the membrane separators, which rely widely on polymers. These architectural components play a key-role in facilitating the transport of ions (i.e. protons) between the (compartments containing the) electrodes and therefore, their properties substantially influence the overall BES performance. This article aims presenting an up-to-date survey on the important accomplishments and promising outlooks with polymer-based membranes (both porous/non-porous, charged/uncharged) applied in BES (first and foremost microbial fuel cells, MFCs) that could drive this technology towards enhanced efficiency. Because of the interdisciplinary concept of BES, it attracts attention from scientists and engineers involved in environmental biotechnology, microbial electrochemistry and applied material sciences and as a result, this review paper would target the audience of these fields with particular interest on the progress with membrane separators fabricated with various polymeric materials.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Critical membrane separator properties in BES are assessed. </LI> <LI> Techniques for membrane/separator characterization are outlined. </LI> <LI> Various polymer-based membranes/separators used in BES are discussed. </LI> <LI> Status and challenges for membrane development in BES are evaluated. </LI> </UL> </P>

Effects of anti-foaming agents on biohydrogen production

Sivagurunathan, Periyasamy,Anburajan, Parthiban,Kumar, Gopalakrishnan,Bakonyi, Pé,ter,Nemestó,thy, Ná,ndor,Bé,lafi-Bakó,, Katalin,Kim, Sang-Hyoun Elsevier 2016 Bioresource technology Vol.213 No.-

<P><B>Abstract</B></P> <P>The effects of antifoaming agents on fermentative hydrogen production using galactose in batch and continuous operations were investigated. Batch hydrogen production assays with LS-303 (dimethylpolysiloxane), LG-109 (polyalkylene), LG-126 (polyoxyethylenealkylene), and LG-299 (polyether) showed that the doses and types of antifoaming agents played a significant role in hydrogen production. During batch tests, LS-303 at 100μL/L resulted in the maximum hydrogen production rate (HPR) and hydrogen yield (HY) of 2.5L/L-d and 1.08mol H<SUB>2</SUB>/mol galactose<SUB>added</SUB>, respectively. The following continuously stirred tank reactor operated at 12h HRT with LS-303 at 100μL/L showed a stable HPR and HY of 4.9L/L-d and 1.17mol H<SUB>2</SUB>/mol galactose<SUB>added</SUB>, respectively, which were higher than those found for the control reactor. Microbial community analysis supported the alterations in H<SUB>2</SUB> generation under different operating conditions and the stimulatory impact of certain antifoaming chemicals on H<SUB>2</SUB> production was demonstrated.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Antifoaming agents types and dosages influence the hydrogen productivity. </LI> <LI> LS-303 and other agents at 100μL/L showed stimulatory effects on H<SUB>2</SUB> production. </LI> <LI> Increased cluster I <I>Clostridium</I> content at the low dosage attributed high H<SUB>2</SUB> yield. </LI> </UL> </P>

Analysing of the Yarn Pull-out Process for the Characterization of Reinforcing Woven Fabrics

Ábris D. Virág,László M. Vas,Péter Bakonyi,Marianna Halász 한국섬유공학회 2019 Fibers and polymers Vol.20 No.9

Our goal was to test the theoretical yarn pull-out model we developed. We examined eight glass woven fabricsfrom the same manufacturer. We compared the values provided by the theoretical model with the measurement results, andbased on these, we determined the relationship between the length of the yarn embedded in the woven fabric and the tensileforce acting on the yarn. All in all, we concluded that the model described the yarn pull-out process well; hence it can beapplied to more complex woven fabric models and simulations.

SCON—a Short Conditional intrON for conditional knockout with one-step zygote injection

Wu Szu-Hsien Sam,Lee Heetak,Szép-Bakonyi Réka,Colozza Gabriele,Boese Ayse,Gert Krista R.,Hallay Natalia,Lee Ji-Hyun,Kim Jihoon,Zhu Yi,Linssen Margot M.,Pilat-Carotta Sandra,Hohenstein Peter,Theussl Ha 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

The generation of conditional alleles using CRISPR technology is still challenging. Here, we introduce a Short Conditional intrON (SCON, 189 bp) that enables the rapid generation of conditional alleles via one-step zygote injection. In this study, a total of 13 SCON mouse lines were successfully generated by 2 different laboratories. SCON has conditional intronic functions in various vertebrate species, and its target insertion is as simple as CRISPR/Cas9-mediated gene tagging.

Evaluation of a membrane permeation system for biogas upgrading using model and real gaseous mixtures: The effect of operating conditions on separation behaviour, methane recovery and process stability

Nemestó,thy, Ná,ndor,Bakonyi, Pé,ter,Szentgyö,rgyi, Eszter,Kumar, Gopalakrishnan,Nguyen, Dinh Duc,Chang, Soon Woong,Kim, Sang-Hyoun,Bé,lafi-Bakó,, Katalin Elsevier 2018 JOURNAL OF CLEANER PRODUCTION Vol.185 No.-

<P><B>Abstract</B></P> <P>In this paper, the enrichment of methane by membrane technology was studied by employing (i) a model as well as (ii) a real biogas mixture produced on a laboratory-scale. Thereafter, the endurance of the process was tested at an existing biogas plant. The commercial gas separation module under investigation contained hollow fiber membranes with a polyimide selective layer. During the measurements, the effect of critical factors (including the permeate-to-feed pressure ratio and the splitting factor) was sought in terms of the (i) CH<SUB>4</SUB> content on the retentate-side and (ii) CH<SUB>4</SUB> recovery, which are important measures of biogas upgrading efficiency. The results indicated that a retentate with 93.8 vol% of CH<SUB>4</SUB> – almost biomethane (>95 vol% of CH<SUB>4</SUB>) quality – could be obtained using the model gas (consisting of 80 vol% of CH<SUB>4</SUB> and 20 vol% of CO<SUB>2</SUB>) along with 77.4% CH<SUB>4</SUB> recovery in the single-stage permeation system. However, in the case of the real biogas mixture, ascribed primarily to inappropriate N<SUB>2</SUB>/CH<SUB>4</SUB> separation, the peak methane concentration noted was only 80.7 vol% with a corresponding 76% CH<SUB>4</SUB> recovery. Besides, longer-term experiments revealed the adequate time-stability of membrane purification, suggesting such a process is feasible under industrial conditions for the improvement of biogas quality.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Membrane gas separation was applied for biogas enrichment. </LI> <LI> Polyimide membrane was investigated to deliver biomethane. </LI> <LI> Significant variables affecting membrane performance were evaluated. </LI> <LI> Process efficiency was dependent on gas composition (model vs. real biogas). </LI> <LI> The gas permeation was steady in longer-terms using real biogas mixture. </LI> </UL> </P>

Assessment via the modified gompertz-model reveals new insights concerning the effects of ionic liquids on biohydrogen production

Nemestó,thy, Ná,ndor,Bakonyi, Pé,ter,Ró,zsenberszki, Tamá,s,Kumar, Gopalakrishnan,Koó,k, Lá,szló,Kelemen, Gá,bor,Kim, Sang-Hyoun,Bé,lafi-Bak Elsevier 2018 International journal of hydrogen energy Vol.43 No.41

<P><B>Abstract</B></P> <P>Lignocellulosic biofuel, in particular hydrogen gas production is governed by successful feedstock pretreatment, hydrolysis and fermentation. In these days, remarkable attention is paid to the use of ionic liquids to make the fermentable regions of lignocellulose biomass more accessible to the biocatalysts. Although these compounds have great potential for this purpose, their presence during the consecutive fermentation stage may pose a threat on process stability due to certain toxic effects. This, however, has not been specifically elaborated for dark fermentative biohydrogen generation. Hence, in this work, two common imidazolium-type ionic liquids (1-butyl-3-methylimidazolium acetate, ([bmim][Ac]) and 1-butyl-3-methylimidazolium chloride, ([bmim][Cl])) were employed in mixed culture biohydrogen fermentation to investigate the possible impacts related to their presence and concentrations. The batch assays were evaluated comparatively via the modified Gompertz-model based on the important parameters characterizing the process, namely the biohydrogen production potential, maximum biohydrogen production rate and lag-phase time.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The impact of imidazolium-type ionic liquids on biohydrogen formation was tested. </LI> <LI> The batch biohydrogen production process was evaluated kinetically. </LI> <LI> Both [bmim][Ac] and [bmim][Cl] affected the biohydrogen formation performance. </LI> <LI> The anion part of ionic liquids ([Ac]<SUP>-</SUP> vs. [Cl]<SUP>-</SUP>) demonstrated notable effect. </LI> </UL> </P>

Microstructure and morphology of electrodeposited Ni–P alloys treated by high energy surface mechanical attrition

Ádám Révész,László Péter,Péter J. Szabó,Péter Szommer,Imre Bakonyi 한국물리학회 2012 Current Applied Physics Vol.12 No.1

Fully amorphous NieP layer electrodeposited onto a Cu plate was subjected to severe plastic deformation using surface mechanical attrition treatment in a high energy SPEX 8000 shaker mill. Two series of experiments using different milling conditions (series I: 20 6.35-mm balls; series II: 100 1.59-mm balls)were carried out to explore the mechanism of the process and to investigate the structure of the developed coatings. The evolution of the microstructure and mechanical properties of the NieP layer after the deformation process was studied by x-ray diffraction, scanning electronmicroscopy and hardness measurements.We demonstrate that the different mechanical treatments controllably influence the mechanical behavior of the NieP metallic glass coating. When the vial of the mill is loaded with larger balls, deformation-induced Ni3P compound particles form in the amorphous matrix resulting in a hard (HV ¼ 17 GPa) but non-uniform coating. In the case of milling with many small balls, the increase in the surface hardness is considerably lower (7 GPa) as a consequence of reduced impact energy.

Improvement of hydrogen fermentation of galactose by combined inoculation strategy

Sivagurunathan, P.,Anburajan, P.,Kumar, G.,Arivalagan, P.,Bakonyi, P.,Kim, S.H. Society for Bioscience and Bioengineering, Japan ; 2017 Journal of bioscience and bioengineering Vol.123 No.3

<P>This study evaluated the feasibility of anaerobic hydrogen fermentation of galactose, a red algal biomass sugar, using individual and combined mixed culture inocula. Heat-treated (90 degrees C, 30 min) samples of granular sludge (GS) and suspended digester sludge (SDS) were used as inoculum sources. The type of mixed culture inoculum played an important role in hydrogen production from galactose. Between two inocula, granular sludge showed higher hydrogen production rate (HPR) and hydrogen yield (HY) of 2.2 L H2/L-d and 1.09 mol H2/mol galactoseadded, respectively. Combined inoculation (GS + SDS) led to an elevated HPR and HY of 3.1 L H2/L-d and 1.28 mol H2/mol galactoseadded, respectively. Acetic and butyric acids are the major organic acids during fermentation. Quantitative polymerase chain reaction (qPCR) revealed that the mixed culture generated using the combined inoculation contained a higher cluster I Clostridium abundance than the culture produced using the single inoculum. (C) 2016, The Society for Biotechnology, Japan. All rights reserved.</P>