http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Anburajan, Parthiban,Pugazhendhi, Arivalagan,Park, Jong-Hun,Sivagurunathan, Periyasamy,Kumar, Gopalakrishnan,Kim, Sang-Hyoun Elsevier Applied Science 2018 Bioresource Technology Vol.247 No.-
<P><B>Abstract</B></P> <P>This study investigated the effect of 5-hydroxymethylfurfural (5-HMF) on high-rate continuous fermentative H<SUB>2</SUB> production in a lab-scale fixed bed reactor (FBR) inoculated with mixed culture granules and fed with 15g/L galactose at a hydraulic retention time of 6h and at 37°C. During the 83days of operation, 5-HMF up to 2.4g/L was spiked into the feedstock. The maximum hydrogen production performance of 26.6L/L-d and 2.9mol H<SUB>2</SUB>/mol galactose<SUB>added</SUB> were achieved at 5-HMF concentration of 0.6g/L. 5-HMF concentration exceeding 0.9g/L not only inhibited hydrogen production but also affected the biofilm structure and microbial community population. However, when 5-HMF was eliminated from the feedstock, the performance and microbial community population were rapidly recovered.</P> <P><B>Highlights</B></P> <P> <UL> <LI> 5-HMF at or over 0.9g/L inhibited continuous H<SUB>2</SUB> production from galactose. </LI> <LI> 5-HMF shifted dominant groups from <I>Clostridium butyricum</I> to <I>Lactobacillus</I>. </LI> <LI> Inhibition to continuous culture was milder than that to batch culture. </LI> <LI> Inhibition was rapidly overcome when 5-HMF was eliminated from feedstock. </LI> </UL> </P>
Anburajan, P.,Park, J.H.,Sivagurunathan, P.,Pugazhendhi, A.,Kumar, G.,Choi, C.S.,Kim, S.H. Society for Bioscience and Bioengineering, Japan ; 2017 Journal of bioscience and bioengineering Vol.124 No.3
<P>This study examined the mesophilic continuous biohydrogen fermentation from galactose and glucose mixture with an initial substrate concentration of 15 g/L (galactose 12 g/L and glucose 3 g/L) as a resembling carbon source of pre-treated red algal hydrolyzate. A fixed bed reactor was fed with the sugar mixture at various hydraulic retention times (HRTs) ranging 12 to 1.5 h. The maximum hydrogen production rate of 52.6 L/L-d was found at 2 h HRT, while the maximum hydrogen yield of 2.3 +/- 0.1 mol/mol hexose(added), was achieved at 3 h HRT. Microbial communities and species distribution were analyzed via quantitative polymerase chain reaction (qPCR) and the dominant bacterial population was found as Clostridia followed by Lactobacillus sp. Packing material retained higher 16S rRNA gene copy numbers of total bacteria and Clostridium butyricum fraction compared to fermentation liquor. The finding of the study has demonstrated that H-2 production from galactose and glucose mixture could be a viable approach for hydrogen production. (C) 2017, The Society for Biotechnology, Japan. All rights reserved.</P>
Anburajan, Parthiban,Yoon, Jeong-Jun,Kumar, Gopalakrishnan,Park, Jong-Hun,Kim, Sang-Hyoun Elsevier 2019 INTERNATIONAL JOURNAL OF HYDROGEN ENERGY - Vol.44 No.4
<P><B>Abstract</B></P> <P>In this study, a novel inoculation method to mitigate the inhibition of 5-hydroxymethylfurfural (5-HMF) is proposed. Acid algae hydrolysate containing 1.5 g 5-HMF/L and 15 g hexose/L hexose was fed to a continuous fixed bed reactor (C-FBR) partially packed with hybrid-immobilized beads. The inoculation method enabled a high rate of H<SUB>2</SUB> production, due to the reduction of 5-HMF inhibition and enhanced biofilm formation. Maximum hydrogen production was achieved at a hydraulic retention time of 6 h with a hydrogen production rate (HPR) of 20.0 ± 3.3 L H<SUB>2</SUB>/L-d and a hydrogen yield (HY) of 2.3 ± 0.4 mol H<SUB>2</SUB>/mol hexose <SUB>added</SUB>. Butyrate and acetate were the major soluble metabolic products released during fermentation. Quantitative real-time polymerase chain reaction analysis revealed that <I>Clostridium butyricum</I> comprised 94.3% of the total bacteria, which was attributed to the high rate of biohydrogen production.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Pore of support material was packed with hybrid immobilized beads. </LI> <LI> The new inoculation method mitigated the inhibition of 5-HMF on bio-H<SUB>2</SUB>. </LI> <LI> H<SUB>2</SUB> production of 20.0 L/L-d was achieved with 1.5 g 5-HMF/L. </LI> </UL> </P>
Effects of anti-foaming agents on biohydrogen production
Sivagurunathan, Periyasamy,Anburajan, Parthiban,Kumar, Gopalakrishnan,Bakonyi, Pé,ter,Nemestó,thy, Ná,ndor,Bé,lafi-Bakó,, Katalin,Kim, Sang-Hyoun Elsevier 2016 Bioresource technology Vol.213 No.-
<P><B>Abstract</B></P> <P>The effects of antifoaming agents on fermentative hydrogen production using galactose in batch and continuous operations were investigated. Batch hydrogen production assays with LS-303 (dimethylpolysiloxane), LG-109 (polyalkylene), LG-126 (polyoxyethylenealkylene), and LG-299 (polyether) showed that the doses and types of antifoaming agents played a significant role in hydrogen production. During batch tests, LS-303 at 100μL/L resulted in the maximum hydrogen production rate (HPR) and hydrogen yield (HY) of 2.5L/L-d and 1.08mol H<SUB>2</SUB>/mol galactose<SUB>added</SUB>, respectively. The following continuously stirred tank reactor operated at 12h HRT with LS-303 at 100μL/L showed a stable HPR and HY of 4.9L/L-d and 1.17mol H<SUB>2</SUB>/mol galactose<SUB>added</SUB>, respectively, which were higher than those found for the control reactor. Microbial community analysis supported the alterations in H<SUB>2</SUB> generation under different operating conditions and the stimulatory impact of certain antifoaming chemicals on H<SUB>2</SUB> production was demonstrated.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Antifoaming agents types and dosages influence the hydrogen productivity. </LI> <LI> LS-303 and other agents at 100μL/L showed stimulatory effects on H<SUB>2</SUB> production. </LI> <LI> Increased cluster I <I>Clostridium</I> content at the low dosage attributed high H<SUB>2</SUB> yield. </LI> </UL> </P>
Enhanced Fermentative Hydrogen Production from Galactose using Bioaugmentation Strategy
( Periyasamy Sivagurunathan ),( Anburajan Parthiban ),( Gopalakrishnan Kumar ),( Sang Hyoun Kim ) 한국폐기물자원순환학회(구 한국폐기물학회) 2015 한국폐기물자원순환학회 3RINCs초록집 Vol.2015 No.-
This study evaluated the feasibility of anaerobic hydrogen fermentation of galactose (red algal biomass sugar) using individual and bioaugmented mixed cultures. Heat-treated (80ºC 30 min) samples of granular sludge and digester sludge were used as inoculum source and their individual and bioaugmentive role on hydrogen production were examined in batch tests. Among the tested individual inoculum, granular sludge provided the maximum hydrogen production rate (HPR) and hydrogen yield (HY) of 2.8 L/L-d and 0.64 mol/mol galctoseadded. Bioaugmented (granular sludge+ digester sludge) mixed culture showed a peak HPR and HY of 3.2 L/L-d and 0.84 mol/mol galctose added, respectively. The results showed that there was a significant improvement in hydrogen production performances were observed when the individual hat-treated mixed cultures were added together. The hydrogen content was maintained over ~52% in both individual and bioaugmented cultures. The sugar consumption rate was more than 90% in all cases. The soluble metabolic products analysis revealed that butyrate and acetate was the major dominant metabolic products. This study proved that bioaugmentation could be a feasible strategy for improving hydrogen production from galactose and to solve the energy issue in the future.
Pugazhendhi, Arivalagan,Anburajan, Parthiban,Park, Jong-Hun,Kumar, Gopalakrishnan,Sivagurunathan, Periyasamy,Kim, Sang-Hyoun Elsevier 2017 International journal of hydrogen energy Vol.42 No.45
<P><B>Abstract</B></P> <P>This paper describes the continuous biohydrogen production in a mesophilic fixed-bed reactor using anaerobic digester sludge as an inoculum. Hydraulic retention times (HRTs) were decreased stepwise from 12 to 1.5 h, while 15 g/L of glucose was used as the model substrate. The peak hydrogen production performance was found at 1.5 h HRT with the hydrogen yield (HY) of 2.3 mol H<SUB>2</SUB>/mol glucose<SUB>added</SUB> and the hydrogen production rate (HPR) of 78 L H<SUB>2</SUB>/L-d. Butyrate and acetate were the major soluble metabolic products released during the fermentation. Quantitative polymerase chain reaction (qPCR) and scanning electron microscopy analyses implied that <I>Clostridium butyricum</I> was dominant in the mixed culture fermentation in all the examined HRTs.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Continuous hydrogen fermentation of glucose by fixed bed reactor has been demonstrated. </LI> <LI> Peak HY and HPR were obtained as 2.3 mol H2/mol glucose<SUB>added</SUB> and 78 L H2/L-d. </LI> <LI> <I>Clostridium butyricum</I> was dominant in the mixed culture fermentation. </LI> </UL> </P>
Mesophilic biogenic H<sub>2</sub> production using galactose in a fixed bed reactor
Sivagurunathan, P.,Anburajan, P.,Park, J.H.,Kumar, G.,Park, H.D.,Kim, S.H. Pergamon Press 2017 International journal of hydrogen energy Vol.42 No.6
<P>This study investigated hydrogen fermentation from galactose in a fixed bed reactor (FBR) under a mesophilic temperature of 37 degrees C. The fixed bed reactor was packed with Lantec HD-Q-PAC material to support biomass growth and microbial assisted granule formation over the time course of the reactor operation. Hydraulic retention times (HRT) of 1.5-12 h over 79 days of bioreactor operation were assessed to determine the optimal hydrogen production efficiency of the system. The maximum hydrogen production rate and hydrogen yield of 65.5 L/L/d and 2.60 mol/mol hexoseadaed were achieved at a hydraulic retention time (HRT) of 2 h, which is higher than the reported maximum hydrogen production performance of reactors fed with galactose using other reactor configuration. The major volatile fatty acids formed were butyric and acetic acids. Microbial community analysis by quantitative real time polymerase chain reaction revealed that population changes greatly affected hydrogen production performance. The increase in the bacterial fraction of Lactobacillus spp. over 21.5% at a 1.5 h HRT led to increased lactic acid production up to 1865 mg/L, which deteriorated the reactor performances. Conversely, a bacterial fraction of Clostridium butyricum over 98% was observed when the H-2 production performance and organic acid distribution were recovered using a 2 h HRT. FBR would be a promising reactor configuration to achieve high hydrogen productivity while preventing the wash out of active biomass. (C) 2016 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved.</P>
Sivagurunathan, P.,Anburajan, P.,Kumar, G.,Kim, S.H. Pergamon Press 2016 International journal of hydrogen energy Vol.41 No.46
<P>This study investigated the hydrogen fermentation from galactose in up-flow anaerobic sludge blanket (UASB) reactor under mesophilic temperature of 37 degrees C. The maximum hydrogen production rate (HPR) and hydrogen yield (HY) of 56.8 L/L/d and 2.25 mol/mol galactose added were achieved at a hydraulic retention time (HRT) of 2 h. Further shortening the HRT to 1.5 h, led to the significant drop in the HPR and HY with a value of 48.3 L/L/d and 1.44 mol/mol galactose added, respectively. During the reactor operation, neither external pH adjustment nor recirculation was required, while the pH was maintained in the range of 5.5-6.2 by the carbonate buffer in the nutrient medium. Acetate and butyrate were the major soluble metabolic products (SMPs) formed during the hydrogen fermentation with fewer amounts of lactate and propionate during the peak hydrogen production performances. The results showed that controlling appropriate HRT is prerequisite for the enhancement of hydrogen production performances. (C) 2016 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved.</P>