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Ahmed, Raju,Getachew, Adane Tilahun,Cho, Yeon-Jin,Chun, Byung-Soo Elsevier 2018 FOOD SCIENCE AND TECHNOLOGY -ZURICH- Vol.89 No.-
<P>Collagen extracted from fish skin by using porcine-derived pepsin is not accepted by a large number of people due to religious constrains. The aim of the study was to explore bacterial collagenolytic proteases (CP) to extract fish skin collagen and characterize the extracted collagen. Two bacteria, Bacillus cereus FORC005 and Bacillus cereus FRCY9-2 were isolated. Glycerol and sucrose were suitable carbon sources for FORC005 and FRCY9-2, respectively. Response surface methodology (RSM) was employed to optimize pH, temperature (T) and concentration of carbon source (CC) for producing maximum CP. The temperature and pH were the most influencing factors for producing CP by FORC005 and FRCY9-2, respectively. The total yield of collagen by FORC005 and FRCY9-2 CP treatments combined with acid soluble collagen were 188 g/kg and 177 g/kg, respectively of skin (dry matter basis). The SDS-PAGE band patterns, FT-IR spectra and amino acid compositions indicated that all extracted collagens were type I collagen. The CPs produced from the bacteria were able to extract collagen from fish skin. This study provides an alternative approach for producing collagen from fish skin using CP.</P>
J.N. Moon,Adane Tilahun Getachew,A.S.M. Tanbirul Haque,Periaswamy Sivagnanam Saravana,Y.J. Cho,David Nkurunziza,B.S. Chun 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.69 No.-
This study was aimed to extract oil from Asiasarum heterotropoides using supercritical carbon dioxide (Sc-CO2) with and without ethanol as co-solvent and to compare the results with conventional extraction solvents. A total of 27 major volatile organic compounds (VOCs) were identified among them eucarvone, 3-carene, safrole, methyleugenol, and camphene were found to be the highest. The solvent Sc-CO2 + ethanol at 250 bar and 50 °C was found to be the best condition to get high yield of oil with 3.54 ± 0.21%. The extracted oil showed 100% of deodorant efficiency within 30 min of reaction with an artificially manufactured odorant.
Monjurul Haq,Truc Cong Ho,Raju Ahmed,Adane Tilahun Getachew,Yeon-Jin Cho,Jin-Seok Park,Byung-Soo Chun 한국공업화학회 2020 Journal of Industrial and Engineering Chemistry Vol.81 No.-
Bacterial collagenolytic protease-extracted Bigeye tuna skin collagen was subjected to catalysts-assistedsubcritical water hydrolysis. The degree of hydrolysis was the highest for hydrolysates obtained usingsodium bicarbonate catalysts. The color and pH values of the hydrolysates varied depending of thecatalysts used. Four different assays of antioxidant activities varied with respect to the various collagenhydrolysates with hydrolysates obtained using sodium bicarbonate catalysts showing the highestactivity. Ten bacterial species were studied to evaluate antibacterial activity among which nine showedpositive results. The average molecular size of the peptides in the obtained collagen hydrolysates variedbetween 300 and 425 Da. The total amount of structural and total amino acids varied between 116.63 and49.51, and 196.86 and 265.68 mg/100 g hydrolysate, respectively. The collagen hydrolysates obtainedshowed enormous potential to be used in the food and pharmaceutical industries.