Effects of Various Addition and Exclusion Time of Glucose on Development of Mouse Two-Cell Embryos

S. B. Park,K. S. Park,T. H. Lee,S. S. Chun,S. S. Kim,H. B. Song 사단법인 한국동물생명공학회 2004 Reproductive & developmental biology Vol.28 No.4

This study was conducted to investigate the effect of various addition and exclusion time of glucose (Control: no addition, A: 24∼72 h, B: 24∼48 h, C: 48∼72 h, D: 0∼72 h, E: 0∼48 h, F: 0∼24 h and 48∼72 h, G: 0∼24 h) on embryonic developmental capacity of 2-cell embryos in mice. Developed blastocysts were assessed for mean cell number by differential staining. The zona-intact blastocyst (ZiB) rates were higher (p 0.05) in group B than control. However, the zona-escape blastocyst (ZeB) rates were not significantly different in all groups. At 72 h, total blastocyst (ZiB + ZeB) formation rates were not significantly different in all groups. The mean cell number was not significantly different among all groups. The inner cell mass (ICM) cell number was higher (p 0.05) in group F than control, group A, B and G. The trophectoderm (TE) cell number was higher (p 0.05) in control than group A and D. The %ICM was higher (p 0.05) in group C, D and F than control. The ICM : TE ratio was not significantly different in all groups. Between control and glucose group, no significant difference was observed in the total blastocysts (ZiB + ZeB) formation rates. Also, no significant difference was observed in the mean cell number, ICM cell number and ICM : TE ratio. However the TE cell number was higher (p 0.05) in control than glucose group and %ICM was higher (p 0.05) in glucose group than control. In conclusion, glucose added in culture medium was not inhibitory on blastocyst formation but glucose added for 48∼72 h in culture medium increases %ICM of blastocysts in mice.

Effects of Various Addition and Exclusion Time of Glucose on Development of Mouse Two-Cell Embryos

Park, S. B.,Park, K. S.,Lee, T. H.,Chun, S. S.,Kim, K. S.,Song, H. B. 한국동물생명공학회(구 한국동물번식학회) 2004 Reproductive & developmental biology Vol.28 No.4

This study was conducted to investigate the effect of various addition and exclusion time of glucose (Control: no addition, A: 24~72 h, B: 24~48 h, C: 48~72 h, D: 0~72 h, E: 0~48 h, F: 0~24 h and 48~72 h, G: 0~24 h) on embryonic developmental capacity of 2-cell embryos in mice. Developed blastocysts were assessed for mean cell number by differential staining. The zona-intact blastocyst (ZiB) rates were higher (p<0.05) in group B than control. However, the zona-escape blastocyst (ZeB) rates were not significantly different in all groups. At 72 h, total blastocyst (ZiB + ZeB) formation rates were not significantly different in all groups. The mean cell number was not significantly different among all groups. The inner cell mass (ICM) cell number was higher (p<0.05) in group F than control, group A, B and G. The trophectoderm (TE) cell number was higher (p<0.05) in control than group A and D. The %ICM was higher (p<0.05) in group C, D and F than control. The ICM : TE ratio was not significantly different in all groups. Between control and glucose group, no significant difference was observed in the total blastocysts (ZiB + ZeB) formation rates. Also, no significant difference was observed in the mean cell number, ICM cell number and ICM : TE ratio. However the TE cell number was higher (p<0.05) in control than glucose group and %ICM was higher (p<0.05) in glucose group than control. In conclusion, glucose added in culture medium was not inhibitory on blastocyst formation but glucose added for 48 ~72 h in culture medium increases %ICM of blastocysts in mice.

Purification and characterization of a novel fibrinolytic α chymotrypsin like serine metalloprotease from the edible mushroom, Lyophyllum shimeji

Moon, S.M.,Kim, J.S.,Kim, H.J.,Choi, M.S.,Park, B.R.,Kim, S.G.,Ahn, H.,Chun, H.S.,Shin, Y.K.,Kim, J.J.,Kim, D.K.,Lee, S.Y.,Seo, Y.W.,Kim, Y.H.,Kim, C.S. Society for Bioscience and Bioengineering, Japan ; 2014 Journal of bioscience and bioengineering Vol.117 No.5

A novel fibrinolytic enzyme was purified from Lyophyllum shimeji, a popular edible mushroom in Asia. The enzyme was purified using combination of anion exchange chromatography on a Mono Q 5/5 column and size exclusion gel filtration chromatography on Superdex 200 100/300 column. This purification protocol resulted 80.9-fold purification of the enzyme and a final yield of 5.7%. The molecular weight of the purified enzyme was estimated to be 21 kDa by SDS-PAGE and size exclusion gel filtration. The N-terminal amino acid sequence was found to be ITFQSASP, which is dissimilar from that of known fibrinolytic enzymes. The purified enzyme was a neutral protease with an optimal reaction pH and temperature of 8.0 and 37<SUP>o</SUP>C, respectively. Enzymatic activity was inhibited by Cu<SUP>2+</SUP> and Co<SUP>2+</SUP>. It was also significantly inhibited by PMSF and TPCK. Furthermore, it was found to exhibit a higher specificity for S-7388, a well-known chymotrypsin chromogenic substrate, indicating chymotrypsin like serine metalloprotease. The relative fibrinolytic activity of 5 μg purified enzyme have two fold more activity than 1 unit/ml of plasmin on fibrin plate. Furthermore, purified enzyme preferentially hydrolyzed the Aα-chain followed by the Bβ- and γ-chain of fibrinogen, which is precursor of fibrin. Therefore, these data suggests that the fibrinolytic enzyme derived from edible mushroom, L. shimeji, might be useful for thrombolytic therapy and preventing thrombotic disease.

Effects of Various Addition and Exclusion Time of Glucose on Development of Mouse Two-Cell Embryos

Park, S. B.,Park, K. S.,Lee, T. H.,Chun, S. S.,Kim, K. S.,Song, H. B. 한국동물번식학회 2004 Reproductive & developmental biology Vol.28 No.4

This study was conducted to investigate the effect of various addition and exclusion time of glucose (Control: no addition, A: 24~72 h, B: 24~48 h, C: 48~72 h, D: 0~72 h, E: 0~48 h, F: 0~24 h and 48~72 h, G: 0~24 h) on embryonic developmental capacity of 2-cell embryos in mice. Developed blastocysts were assessed for mean cell number by differential staining. The zona-intact blastocyst (ZiB) rates were higher (p<0.05) in group B than control. However, the zona-escape blastocyst (ZeB) rates were not significantly different in all groups. At 72 h, total blastocyst (ZiB + ZeB) formation rates were not significantly different in all groups. The mean cell number was not significantly different among all groups. The inner cell mass (ICM) cell number was higher (p<0.05) in group F than control, group A, B and G. The trophectoderm (TE) cell number was higher (p<0.05) in control than group A and D. The %ICM was higher (p<0.05) in group C, D and F than control. The ICM : TE ratio was not significantly different in all groups. Between control and glucose group, no significant difference was observed in the total blastocysts (ZiB + ZeB) formation rates. Also, no significant difference was observed in the mean cell number, ICM cell number and ICM : TE ratio. However the TE cell number was higher (p<0.05) in control than glucose group and %ICM was higher (p<0.05) in glucose group than control. In conclusion, glucose added in culture medium was not inhibitory on blastocyst formation but glucose added for 48 ~72 h in culture medium increases %ICM of blastocysts in mice.

Ti-Ni-Cu 형상기억합금의 상변태 및 초탄성에 미치는 가공열처리의 영향

이오연,박영구,쳔병선 ( O . Y . Lee,Y . K . Park,B . S . Chun ) 한국열처리공학회 1994 熱處理工學會誌 Vol.7 No.4

Transformation behavior and superelastic behavior of Ti-Ni-Cu alloys with various Cu content has been investigated by means of electrical resistivity measurement, X-ray diffraction, tensile test and transmission electron microscopy. Two types of heat treatment are given to the specimens : i) Solutions treatment. ii) thermo-mechanical treatment. The transformation sequence in solution treated Ti-Ni-Cu Alloys substituted by Cu for Ni up to 5at.% occurs to B2◎B19` and it proceeds in two stages by addition of 10at.%Cu, i. e, B2◎B19◎B19`. Also, it has been found that Ti-30Ni-20Cu alloy transformed in one stage : B2◎B19. The thermo-mechanically treated Ti-47Ni-3Cu alloy transformed in two stages : B2◎rhomboheral phase◎B19`, while transformation sequence in Ti-45Ni-5Cu and Ti-40Ni-l0Cu alloy transformed as same as solution treated specimens. The critical stress for inducing slip deformation in solution treated and thermo-mechanically treated Ti-40Ni-l0Cu alloy is about 90㎫ and 320㎫ respectively.

Diamond 박막 성장에 미치는 Si 표면 영향의 AES에 의한 연구

이철로(C. R. Lee),신용현(Y. H. Shin),임재영(J. Y. Leem),정광화(K. H. Chung),천병선(B. S. Chun) 한국진공학회(ASCT) 1993 Applied Science and Convergence Technology Vol.2 No.2

Si 기판 표면상태 변화와 관련된 핵생성 자유에너지 증가에 따른 다이아몬드 박막성장 거동을 관찰하였다. 표면 연마조건 변화에 따른 3가지 기판(A-Si, B-Si, C-Si) 위에 동일한 성장조건으로 다이아몬드를 성장하였으며, 이때 형상인자와 관련된 자유에너지 관계는 ΔG_(A-Si)<ΔG_(B-Si)<ΔG_(C-Si)이다. AES, SEM, XRD, RHEED에 의해 각각의 박막 A, B, C를 조사한 결과, 핵생성 자유에너지가 가장 적은 A 박막은 (100) (110) 면이 지배적인 고품위 다이아몬드 박막이다. 자유에너지가 A에 비해 다소 적은 B 박막은 (111) 면이 지배적인 8면체 다이아몬드 박막이고, 자유에너지가 가장 적은 C 박막은 흑연이 많이 함유된 구상의 다이아몬드이다. The effect of nucleation free energy related to Si surface states on diamond film growth behavior has been studied. At first, the three kinds of diamond thin films (A, B, C) were deposited on various Si substrates (A-Si, B-Si, C-Si) whose surfaces were polished with 1 ㎛ diamond paste, 6 ㎛ Al₂O₃ powder and 12 ㎛ Al₂O₃ powder respectively. And then, relative nucleation free energy calculated is ΔG_(A-Si)<ΔG_(B-Si)<ΔG_(c-Si) Although there are some difference in grain size, shape and nucleation site, the thin films on A-Si and B-Si were diamond including a small amount of DLC which was confirmed by AES, SEM, XRD and RHEED. Namely, the diamonds of films (B) were not nucleated in scratches but in dents and larger in grain size compare with the film (C) of which diamond were nucleated not only scratches but also dents. And, the sphere diamond which is not general shape was grown on C-Si. After all, the sphere was turned out to be the diamond including much graphite as a result of the AES in situ depth profiling. Consequently, the diamond shape and quality grown on Si were changed from the crystal which the (100) and (110) planes were predominent to the crystal in which (111) plane was predominent, and next to sphere shape diamond including much graphite according as the nucleation free energy increases.

Strain path effects on the microstructure evolution and mechanical properties of Zr702

Cao, W.Q.,Yu, S.H.,Chun, Y.B.,Yoo, Y.C.,Lee, C.M.,Shin, D.H.,Hwang, S.K. Elsevier 2005 Materials science & engineering. properties, micro Vol.395 No.1

<P><B>Abstract</B></P><P>A commercial-purity Zr702 was grain-refined from 20μm to 0.2–0.5μm by equal channel angular pressing. Grain refinement was most evident in the first pass but was insignificant during the subsequent passes. Two microstructural characteristics evolved: a lamellar structure and an equiaxed subgrain structure during the route A pressing and the route B<SUB>C</SUB> pressing, respectively. Due to the rotation of specimen in between passes, two sets of geometrically necessary boundaries were formed during the route B<SUB>C</SUB> pressing. The mechanism of grain refinement during the route A pressing was evolution of high-angle geometrically necessary boundaries from the low-angled ones, while that during the route B<SUB>C</SUB> pressing was decomposition and rearrangement of pre-existing boundaries. The yield stress of severely deformed specimens increased with the reducing grain size according to a Hall–Petch relationship.</P>

Structural and magnetic properties of epitaxial Co₂FeAl films grown on MgO substrates for different growth temperatures

Chun, B.S.,Kim, K.H.,Leibing, N.,Serrano-Guisan, S.,Schumacher, H.W.,Abid, M.,Chu, I.C.,Mryasov, O.N.,Kim, D.K.,Wu, H.C.,Hwang, C.,Kim, Y.K. Elsevier Science 2012 ACTA MATERIALIA Vol.60 No.19

We report the correlation between the crystalline structure, electronic structure and magnetic properties of Co<SUB>2</SUB>FeAl films as a function of growing temperature both experimentally and theoretically. The Co<SUB>2</SUB>FeAl film grown at room temperature is initially in the partially disordered B2 state, but then it gains a much higher ordered structure with increasing growing temperature due to its transition from short-range to long-range crystallographic order by surface diffusion. Electron energy loss spectroscopy measurements reveals that the increase in the I(L3)/I(L2) ratio of Co can be attributed to the enhanced ferromagnetic exchange interaction between neighboring Co atoms and the fact that the Co contribution is more dominant than the Fe contribution. As the growing temperature increases, many more unoccupied 3d states in Co are observed, hence the Gilbert damping constant increases due to a strong spin-orbit interaction. We also present the results of highly accurate quasiparticle self-consistent GW calculations and confirm that Co<SUB>2</SUB>FeAl in an ideal L2<SUB>1</SUB> structure is indeed a half-metal with a well-defined band gap in the minority spin channel.

Sweet's Syndrome Associated with Acute Erythema Nodosum

Chang, S. N Korean Dermatological Association 1998 Annals of Dermatology Vol.10 No.3

A Case of Maffucci's Syndrome Associated with Spindle-cell Hemangioendothelioma

Lee, K. S Korean Dermatological Association 1998 Annals of Dermatology Vol.10 No.3