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이승구,노현수,홍승표,이규종,왕지원,태동년,엄기남,방상구,김영준,성문희,Lee, Seung-Goo,Ro, Hyeon-Su,Hong, Seung-Pyo,Lee, Kyu-Jong,Wang, Ji-Won,Tae, Dong-Nyeon,Uhm, Ki-Nam,Bang, Sang-Gu,Kim, Young-Jun,Sung, Moon-Hee 한국미생물 · 생명공학회 1996 한국미생물·생명공학회지 Vol.24 No.1
By using the ${\beta}$-tyrosinase of Citrobacter freundii KCT2006, which was cloned and overexpressed in Escherichia coli, 3,4-dihydroxy phenyl-L-alanine (L-DOPA) was synthesized efficiently from pyrocatechol, sodium pyruvate, and ammonium acetate. Optimal temperature and pH for the reaction were determined to be about 18$^{\circ}C$ and 8.5, respectively. The effects of substrate concentrations were also examined at different concentrations of ammonium acetate, sodium pyruvate, and pyrocatechol. Ammoniumacetate and sodium pyruvate increased the reaction rate until the concentrations reached to 300mM and 50mM, respectively. Although pyrocatechol showed the optimal concentration at 20mM, it was controlled between 20mM and 50mM to avoid the depletion of substrate during the enzymatic synthesis. Meanwhile the synthetic rate was improved about 20% when ethanol was included in the reaction solution. Based on above results, a reaction medium for the productin of L-DOPA was prepared and incubated with 1 unit/ml of ${\beta}$-tyrosinase. Pyrocatechol and sodium pyruvate was added to the reaction solutin intermittently to avoid the substrate depletion during the enzymatic reaction. After 24 hour of reaction, 31.6g/l of L-DOPA was accumulated in the reaction solution as soluble and precipitated ones and the conversion yield was about 85.2%.
노언주(Eon Joo Roh),박시향(Si Hyang Park),황수미(Su Mi Hwang),노현수(Hyeon Su Ro),김병관(Byung Kwan Kim) 한국유화학회 2009 한국응용과학기술학회지 Vol.27 No.4
In this study, we evaluated the anti-oxidative, anti-wrinkle and whitening effect of Duchesnea indica (Andr.) Focke extract. Duchesnea indica (Andr.) Focke was extracted by two different solvents which were n-hexane and ethyl acetate. The anti-oxidant activity was measured by free radical scavenging activity using DPPH (1,1-diphenyl-2-picrylhydrazyl radical). And the inhibitory activities of tyrosinase for whitening effect and collagenase for anti-wrinkle were investigated. For anti-oxidant activity and whitening activity, ethyl acetate fraction of Duchesnea indica (Andr.) Focke extract showed more significant activity than n-hexane fraction of Duchesnea indica (Andr.) Focke extract. For anti-wrinkle activity, ethyl acetate fraction of Duchesnea indica (Andr.) Focke extract exhibited strong inhibition effects compared with reference. Therefore, Duchesnea indica (Andr.) Focke extract may be useful as a new antioxidant and anti-aging agent.
노언주 ( Eon Joo Roh ),박시향 ( Si Hyang Park ),황수미 ( Su Mi Hwang ),노현수 ( Hyeon Su Ro ),김병관 ( Byung Kwan Kim ) 한국유화학회 2010 한국응용과학기술학회지 Vol.27 No.4
In this study, we evaluated the anti-oxidative, anti-wrinkle and whitening effect of Duchesnea indica (Andr.) Focke extract. Duchesnea indica (Andr.) Focke was extracted by two different solvents which were n-hexane and ethyl acetate. The anti-oxidant activity was measured by free radical scavenging activity using DPPH (1,1-diphenyl-2-picrylhydrazyl radical). And the inhibitory activities of tyrosinase for whitening effect and collagenase for anti-wrinkle were investigated. For anti-oxidant activity and whitening activity, ethyl acetate fraction of Duchesnea indica (Andr.) Focke extract showed more significant activity than n-hexane fraction of Duchesnea indico (Andr,) Focke extract. For anti-wrinkle activity, ethyl acetate fraction of Duchesnea indico (Andr.) Focke extract exhibited strong inhibition effects compared with reference. Therefore, Duchesnea indico (Andr.) Focke extract may be useful as a new antioxidant and anti-aging agent.
보문 : 큰느타리버섯 유전체내 LTR Retrotransposon 유전자 탐색 및 특성연구
김신일 ( In Il Kim ),레귀방 ( Quy Vang Le ),김선미 ( Sun Mi Kim ),노현수 ( Hyeon Su Ro ) 한국균학회 2014 韓國菌學會誌 Vol.42 No.1
Transposable elements (TEs) are mobile DNA elements that often cause mutations in genes and alterations in the chromosome structure. In order to identify and characterize transposable elements (TEs) in Pleurotus eryngii, a TE-enriched library was constructed using two sets of TE-specific degenerated primers, which target conserved sequences of RT and RVE domains in fungal LTR retrotransposons. A total of 256 clones were randomly chosen from the library and their insert sequences were determined. Comparative investigation of the insert sequences with those in repeat element database, Repbase, revealed that 71 of them were found to be TE-related fragments with significant similarity to LTR retrotransposons from other species. Among the TE sequences, the 70 TEs were Gypsy-type LTR retrotransposons, including 20 of MarY1 from Tricholoma matsutake, 26 of Gypsy- 8_SLL from Serpula lacrymans, and 16 of RMER17D_MM from mouse, whereas a single sequence, Copia-48-PTR, was found as only Copia-type LTR retrotransposon. Southern blot analysis of the HindIII-digested P. eryngii genomic DNA showed that the retrotransposon sequences similar to MarY1 and Gypsy-8_SLL were contained as high as 14 and 18 copies per genome, respectively, whereas other retrotransposons were remained low. Moreover, both of the two Gypsy retrotransposons were expressed in full length mRNA as shown by Northern blot analysis, suggesting that they were functionally active retrotransposons.
느티만가닥버섯의 신품종에 대한 endo-, exo-cellular 효소 활성도의 비교
Chang-Yun Lee(이창윤),Ho-Sung Song(송호성),Hyeon-Su Ro(노현수),Ju-Ri Woo(우주리),Young-Hyun You(유영현),Jong-Guk Kim(김종국) 한국생명과학회 2012 생명과학회지 Vol.22 No.6
본 연구는 새롭게 개발된 느티만가닥버섯의 6개 품종에 대한 형태적, 생리적 특성을 조사하고 endo-, exo-cellular 효소 활성을 측정하기 위해서 수행되었다. 국내 야생종인 Hm3-10과 일본 재배종인 Hm1-1과의 단핵균사 교배를 통하여 343개의 교배 균주를 획득하여 재배를 실시하여 58개 균주를 1차 선발하고 2차로 6개 균주를 선발하였다. 6개 선발 균주를 대상으로 배양 일수 별로 재배를 실시한 결과 배양일수가 80일 이상에서는 재배일수가 19~20일로 단축되어 최적 배양일수를 80일로 결정하였다. 80일 배양일수에서 각 품종별 형태적 특성을 검증한 결과 Hm15-3, Hm15-4, Hm17-5의 3균주가 재배에 적합한 균주로 판명되었다. 각 균주의 endo-cellular 효소 활성도를 측정한 결과, α-amylase의 효소 활성도가 73.9~102,2 unit/㎎ protein으로 가장 높았으며, chitinase 의 효소활성도가 8.1~13.1 unit/㎎ protein으로 측정되었다. Exo-cellular효소 활성도를 측정한 결과, α-amylase의 효소활성도가 5,292~1,184 unit/㎎ protein으로 가장 높았으며, CMCase와 Xylanase의 효소 활성도가 각각 1,140~245 unit/㎎ protein, 94~575 unit/㎎ protein으로 측정되었다. 그러나 β-glucosidase와 chitinase의 활성도는 비교적 낮은 활성도를 나타내었다. This study was carried out to investigate the morphological and physiological characteristics of six new cultivars of Hypsizygus marmoreus (Hm) and measure endo-, exo-cellular enzyme-specific activity. The domestic wild stain (Hm3-10) and commercial strain in Japan (Hm1-1) were mated by crossing monokaryon mycelia. We gained 58 strains from one of 400 crosses through the 1<SUP>st</SUP> cultivation experiment, and selected six strains from one of 58 strains through the 2<SUP>nd</SUP> cultivation experiment. When six of the selected new strains were grown during several spawn culture periods (60, 70, 80, 90, and 100 days), a spawn culture period of more 80 days was considered to be excellent as being shorter than 19~20 days. Therefore, we determined the period of spawn culture as 80 days. Three strains such as Hm15-3, Hm15-4, and Hm17-5 showed an excellent result. When endo-cellular enzyme activity measured eight strains, we obtained a result of that specific activity of α-amylase at the highest as 73.9~102.2 unit/㎎ protein, and chitinase is lower than α-amylase at 8.1~13.1 unit/㎎ protein. When exo-cellular enzyme activity measured eight strains, we determined the result of that specific activity of α-amylase is the highest at 5,292~1,184 unit/㎎ protein, and CMCase and xylanase were 1,140~245 unit/㎎ protein, 94~575 unit/㎎ protein, compared to each other. However, the enzyme activity of β-glucosidase and chitinase is low.