신세포암으로 오인된 상피모양 신혈관근지방종

하유신(U-Syn Ha),이준하(Jun Ha Lee),이광배(Kwang Bae Lee),최항원(Hang Won Choi),조대행(Dae Haeng Cho),이충범(Choong Bum Lee) 대한비뇨기종양학회 2006 대한비뇨기종양학회지 Vol.4 No.4

흰쥐 콩팥발생과정에서 interleukin-6와 그 수용체의 발현

이충범(Choong-Bum Lee),하유신(Ha-U Syn),조대행(Dae-Haeng Cho),최재연(Jae-Youn Choi),차정호(Jung-Ho Cha) 대한체질인류학회 2007 해부·생물인류학 (Anat Biol Anthropol) Vol.20 No.4

Interleukin-6 (IL-6)와 그 수용체는 신경발생조직에서 중요한 기능을 담당하는 것으로 알려져 있으나, 콩팥의 발생과정에서의 기능은 거의 보고된 바가 없다. 본 연구에서는 IL-6와 그 특이수용체인 IL-6R의 발현을 흰쥐의 발생중인 콩팥에서 조사하였다. 임신 16, 18 및 20일 태아와 출생후 1, 3, 7, 14일의 동물 및 성체의 콩팥을 사용하여 IL-6와 그 수용체의 발현을 면역조직화학법과 in situ hybridization 법으로 각각 관찰하였다. IL-6 단백질은 임신 16일의 태아에서 이미 발현되었으며, 요관싹과 사이조직의 중간엽세포를 포함하여 콩팥단위발생구역의 여러 발생단계의 콩팥단위 구조물에 강한 면역반응성을 보였다. 그러나 성숙된 사구체와 근위세관에서는 발현되지 않았다. 출생후의 동물에서도 콩팥단위발생구역에서의 발현양상은 태아의 것과 같았으며, 성숙된 콩팥세관에서는 원위세관과 집합세관에 강한 면역반응성이, 굵은오름세관에 매우 미약한 면역반응성이 관찰되었고, 이러한 양상은 성체에까지 계속되었다. IL-6R mRNA의 발현은 임신 16일의 태아에서 이미 관찰되며, 출생전 태아 콩팥에서의 발현부위는 IL-6의 것과 유사하였다. 출생후의 성숙된 콩팥단위에서의 IL-6R mRNA 발현은 원위세관, 굵은오름세관 및 집합세관에서 관찰되었으며, 근위세관에서는 S3 부분에서만 발현되었다. 본 연구의 결과로서 IL-6가 그 수용체를 통하여 콩팥단위발생의 초기과정에 관련되어 역할을 담당하고 있는 것으로 보여지며, 성숙된 콩팥에서도 IL-6 단백질과 그 수용체가 계속 발현되는 것으로 보아 일정한 기능을 담당할 것으로 생각된다. Interleukin-6 (IL-6) and its receptor are presumed to play important roles in the developing nervous system. However, little is known about their potential role(s) in the developing kidney. To investigate this, we have studied the expression of IL-6 and its receptor (IL-6R) in the developing rat kidney. Kidneys from 16- (F16), 18- and 20-day-old (F20) fetuses, 1- (P1), 3- (P3), 7- (P7) and 14-day-old (P14) pups, and adult rats were extracted. Renal expressions of IL-6 and its receptors were examined by immunohistochemistry and in situ hybridization respectively. Il-6 protein already appeared in F16. The early stage of renal development before birth, IL-6 showed strong immunoreactivity in the ureteric bud, metanephric mesenchymal cells (MMC) and developing glomerulus. The expression pattern of IL-6 in nephrogenic zone are very similar even after birth. In matured nephron after birth, IL-6 immunoreactivities were detected in distal tubules strongly, and collecting ducts moderately and thick ascending limb weekly. IL- 6R hybridization signals have also already appeared in 16-day old fetal kidney. Before birth, IL-6R mRNAs were expressed in ureteric bud, MMC and developing glomerulus. In the matured nephron after birth, IL-6R mRNA was expressed in the thick ascending limb, distal tubules, collecting ducts and S3 segment of proximal tubule. These results suggest that IL-6 and its receptor may be involved in regulation of nephron formation in nephrogenic zone of rat, and play a role in distal nephron including collecting duct after birth.

한국 남성의 비만과 전립선 비대증, 발기부전과의 연관성

서준상(Jun Sang Suh),조중훈(Jung Hoon Cho),정원식(Won Sik Jeong),하유신(U-Syn Ha),배재현(Jae Hyun Bae),김태효(Tae Hyo Kim),김재헌(Jae Heon Kim),이승환(Seung Hwan Lee),조희주(Hee Ju Cho),유구한(Koo Han Yoo),이형래(Hyung-Lae Lee),장인호(I 대한비뇨기종양학회 2015 대한비뇨기종양학회지 Vol.13 No.3

Purpose: Pathogenically, both erectile dysfunction (ED) and benign prostatic hyperplasia (BPH) are closely related to vascular problems, and vascular problems are closely associated with obesity. This study evaluated the relationships between obesity, BPH, and ED. Materials and Methods: The medical history of male patients, aged ≥40 years, evaluated at urology outpatient clinics of 11 university hospitals in Korea with chief complaints of lower urinary tract symptoms (LUTS)/BPH and ED were retrospectively evaluated. The correlations of medical history, body mass index (BMI), international prostate symptom score (IPSS), prostate volume, International Index of Erectile Function -5 (IIEF-5), and serum testosterone level were assessed. Results: Body mass index showed significant correlations with IPSS (r=0.087, p=0.003), prostate volume (r=0.384, p<0.001), IIEF (r=-0.072, p=0.015), and serum testosterone concentration (r=-0.303, p<0.001). Conclusions: Body mass index correlates with testosterone concentration, prostate volume, and markers of LUTS and ED in men with the latter conditions.

Capsaicin-Induced Apoptosis in MBT-2 Murine Bladder Tumor Cells and Bladder Wall Penetration Effect of the Nano-Encapsulated Capsaicin in Rabbit

Shin Jay Cho(조신제),Hyong Woo Moon(문형우),Woong Jin Bae(배웅진),Yong-Hyun Park(박용현),U-Syn Ha(하유신),Sung-Hoo Hong(홍성후),Sae Woong Kim(김세웅),Ji Youl Lee(이지열) 대한비뇨기종양학회 2019 대한비뇨기종양학회지 Vol.17 No.3

Purpose: In this study, we attempted to characterize capsaicin’s effects with regard to the apoptosis of murine bladder cancer cells (MBT-2) as well as the pharmacodynamics of nano-encapsulated capsaicin formulation for intravesical instillation. Materials and Methods: We assessed the viability of the MBT-2 cells via MTT staining, agarose gel electrophoresis, and flow cytometric apoptosis analysis. Intravesical reagents were instilled into 3 groups of male white New Zealand rabbits. Instillation agents were nano-encapsulated capsaicin dissolved in saline, capsaicin dissolved in saline, and capsaicin dissolved in dimethyl sulfoxide (DMSO). We also determined the pharmacokinetics of urine, plasma, and bladder tissue after intravesical capsaicin instillation. Results: Capsaicin treatment was determined to reduce cell viability in a time- and dose-dependent manner. The capsaicin concentrations in the urine of the rabbits decreased in each of the treatment groups, but we noted a more profound reduction of capsaicin concentration in the nano-encapsulated capsaicin group. Plasma concentrations were definitely lower as compared with the levels measured in the bladder tissue and urine. We noted distinctive differences in patterns of concentration change between the capsaicin with normal saline solution (NSS) or DMSO and the nano-encapsulated capsaicin groups. The concentration of nano-encapsulated capsaicin in the tissue appeared to increase directly with tissue depth. Conclusions: Our results show that capsaicin can induce apoptosis in MBT-2 cells, as well as the excellent permeation properties of nano-encapsulated capsaicin. Treatment with intravesical capsaicin may be a promising alternative therapeutic modality for the treatment of bladder cancer.

임상적으로 무의미한 전립선암의 선별을 위한 자기공명영상 기반의 노모그램 개발

성재우(Jae Woo Sung),신동호(Dongho Shin),박용현(Yong Hyun Park),조혁진(Hyuk Jin Cho),하유신(U-Syn Ha),홍성후(Sung-Hoo Hong),이지열(Ji Youl Lee),김세웅(Sae Woong Kim) 대한비뇨기종양학회 2020 대한비뇨기종양학회지 Vol.18 No.3

Purpose: Various predictive tools have been developed to predict insignificant prostate cancer (PCa) for active surveillance, however, these models cannot reflect all the refinements of current medicine. Thus, we aimed to develop a novel model to predict clinically insignificant PCa incorporating these factors. Materials and Methods: We developed a novel nomogram to predict the probability of insignificant PCa (total tumor volume less than 2.5 cm3, index tumor volume less than 1.3 cm3, organ confined disease and no Gleason pattern 4 or 5) using preoperative data of 790 Korean patients who underwent radical prostatectomy. To evaluate the predictive accuracy, the area under the receiver operating characteristic curve (AUC) was calculated. Next, the predicted probability versus the actual probability was compared. This examination was performed by calibration plotting using 1,000 bootstrap resamples. Results: Of the 790 patients, 668 (84.6%) had clinically significant PCa, and 122 (15.4%) had insignificant PCa. We developed a novel predictive model for clinically insignificant PCa using clinical stage less than T2a, biopsy Gleason sum less than 7, ratio of positive biopsy cores less than 10%, neutrophil-to-lymphocyte ratio, and multiparametric magnetic resonance imaging (mpMRI) visibility, which discriminated patients with clinically insignificant PCa from those with significant PCa with an AUC of 0.9135 (95% confidence interval, 0.9127–0.9143). The calibration plot showed a well-calibrated prediction that had little over- or underestimation. Conclusions: We proposed a novel predictive model for insignificant PCa to more accurately select patients for active surveillance using the results from mpMRI and prebiopsy laboratory marker.

급성 단순성 방광염의 경험적 치료로써 Cefcapene Pivoxil의 유효성

이승주,하유신,김현우,조용현 대한감염학회 2008 감염과 화학요법 Vol.40 No.3

목적 : 급성 단순성 방광염은 일반적으로 원인균에 대한 그 지역의 항생제 내성조사가 이루어지고 그 결과에 따라 항생제가 경험적으로 처방된다. 하지만 최근 우리나라에서 fluoroquinolone계 항생제에 대한 요로감염 원인균의 내성 증가가 보고되고 있어 그 동안 경험적 1차치료제로 선택되었던 fluoroquinolone계 항생제의 역할에 한계가 보이고 있는 실정이다. 이에 저자들은 급성 단순성 방광염 환자에서 cefcapene pivoxil의 미생물학적, 임상적 유효성 및 안전성을 알아보고자 하였다. 재료 및 방법 : 시험은 전향적, 비맹검, 공개, 비비교 임상시험으로 시행되었고 서울지역 2개 종합병원을 방문한 92명의 급성 단순성 방광염 환자에게 cefcapene pivoxil 100 mg을 1일 3회, 5일간 경구 투여하여 1-3일과 2주 후에 추적 관찰하였다. 결과 : 평가가 가능하였던 77명의 환자 중 75명의 환자에서 치료완료 2주 후에 원인균이 제거되어 97.4%의 미생물학적 유효성을 보였으며, 74명의 환자에서 치료 후 증상이 소실되었거나 호전되어 96.1%의 임상적 유효성을 보였다. 약물 관련 이상반응은 8명의 환자에서 나타났으나 이로 인한 치료 중단 사례는 없었으며, 심각한 생화학적 또는 혈액학적 이상반응은 나타나지 않았다. 결론 : Cefcapene 100 mg 1일 3회 5일요법은 미생물학적, 임상적 유효성이 뛰어나며 안전한 치료요법으로 급성단순성 방광염의 경험적 항생제 치료에 적합할 것으로 생각된다. Background : A high prevalence of fluoroquinolone resistance among uropathogens has been observed in Korea. Since empirical antimicrobial therapy should be guided by the proportion of pathogens resistant to the chosen antibiotic, the limitation of fluoroquinolones as the first empirical choice for acute cystitis is noticed in the clinical setting. The present study was undertaken to determine the susceptibility profiles of urinary isolates to cefcapene pivoxil and assess the clinical efficacy in patients with acute uncomplicated cystitis. Materials and Methods : The study was a prospective, open label, non-comparative, non-blinded trial. Ninety-two patients with acute, uncomplicated symptomatic cystitis from two hospitals were treated with cefcapene pivoxil 100 mg tid for 5 days and followed up for 2 weeks. Results : Seventy-seven of the 92 patients were evaluated. Bacteriological eradication of initial pathogens was achieved in 97.4% by 2 weeks after therapy termination. Seventy-four of 77 (96.1%) patients were symptomatically cured or improved after completion of therapy. Drug related adverse experiences were seen in 8 patients. None necessitated cessation of therapy. No Significant biochemical or hematological abnormalities occurred. Conclusion : These results demonstrate the clinical efficacy and safety of empirical 5-day cefcapene pivoxil for acute uncomplicated cystitis.

재발성 요로감염이 있는 소아에서 발견된 선천성 방광게실

서홍진,이동환,이주헌,하유신 大韓泌尿器科學會 2002 Korean Journal of Urology Vol.43 No.11