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생물전환을 통한 음나무발효물의 지표성분 설정 및 동시분석법 검증
장원희,이화영,이봉진,김진만,박선주,Jang, Won Hui,Lee, Wha Young,Lee, Bong Jin,Kim, Jean Man,Park, Seon Ju 한국식품영양학회 2019 韓國食品營養學會誌 Vol.32 No.2
The aim of this study was to select compounds for the standardization of fermented Kalopanax pictus Nakai (KP-F), to develop the analysis method using HPLC-PDA and to perform method validation. KP-F is a fermented powder developed to improve the original physiological activities and create a new functionality. Eleutheroside E, Acanthoside B, and Syringaresinol were selected as the standard compounds and developed our own method for simultaneous analysis. The analyte was isolated using C18 column with a gradient elution of 0.05 M phosphoric acid in water and methanol as the mobile phase at a flow rate of 1 mL/min and detected at 210 nm. As a result, all standard compounds showed good linearity with an $R^2$ (coefficient of correlation) of 1.000 and for the limit of detection range of $0.710{\sim}0.831{\mu}g/mL$, and the limit of quantification as $2.150{\sim}2.520{\mu}g/mL$. The precision was RSD (%) of less than 4.80%, while the accuracy was 4.70%>RSD (%) for the range 102.44~110.48%. In conclusion, the developed analysis method is suitable for the detection of Eleutheroside E, Acanthoside B, and Syringaresinol in KP-F.
연구논문 : 인공피부모델을 이용한 트라넥삼산 제형의 피부투과도 및 자극 평가
박양희 ( Yang Hui Park ),장원희 ( Won Hee Jang ),정경미 ( Kyoung Mi Jung ),이수현 ( Su Hyun Lee ),임경민 ( Kyung Min Lim ) 한국동물실험대체법학회 2012 동물실험대체법학회지 Vol.6 No.1
The skin permeability of active ingredient is one of the most important issues for designing transdermal administration form. At the same time, the formulation should be non-irritant to skin. To evaluate skin permeability and irritancy, various methods have been proposed and ex vivo skin is the tool recommended by regulators. However its use is time consuming and requires numerous human donors. Owing to the similarity to real skin, reconstructed human epidermis model have been recognized as a useful tool. We simultaneously evaluated the permeability and irritancy of tranexamic acid in transdermal formulation using the KeraskinTM of MCTT, a three-dimensional reconstructed human epidermis. We applied 4 transdermal formulations to the KeraskinTM and after incubation for 6 hr, culture medium was collected. KeraskinTM was washed five times with DPBS and MTT assay was performed for measuring cell viability. The collected medium was analyzed by HPLC-MS/MS to determine the tranexamic acid concentration. Cell viability was calculated through dividing with the absorbance of MTT formazan of AHA/BHA non-containing vehicle group. No significant differences were found in four groups. The concentration of tranexamic acid in culture medium was determined by HPLCMS/MS; 11.0 ± 2.5μg/mL in tranexamic acid in AHA/BHA non-containing formulation, 24.6 ± 3.8μg/mL in tranexamic acid in AHA/BHA containing formulation and not detected in vehicle groups. These findings demonstrated that reconstructed human epidermis can be used for the simultaneous evaluation of the skin permeability and irritancy of transdermal drug and cosmetic ingredients or products.
인공피부모델을 이용한 트라넥삼산 제형의 피부투과도 및 자극 평가
박양희 ( Yang Hui Park ),장원희 ( Won Hee Jang ),정경미 ( Kyoung Mi Jung ),이수현 ( Su Hyun Lee ),임경민 ( Kyung Min Lim ) 한국동물실험대체법학회 2012 동물실험대체법학회지 Vol.6 No.1
The skin permeability of active ingredient is one of the most important issues for designing transdermal administration form. At the same time, the formulation should be non-irritant to skin. To evaluate skin permeability and irritancy, various methods have been proposed and ex vivo skin is the tool recommended by regulators. However its use is time consuming and requires numerous human donors. Owing to the similarity to real skin, reconstructed human epidermis model have been recognized as a useful tool. We simultaneously evaluated the permeability and irritancy of tranexamic acid in transdermal formulation using the KeraskinTM of MCTT, a three-dimensional reconstructed human epidermis. We applied 4 transdermal formulations to the KeraskinTM and after incubation for 6 hr, culture medium was collected. KeraskinTM was washed five times with DPBS and MTT assay was performed for measuring cell viability. The collected medium was analyzed by HPLC-MS/MS to determine the tranexamic acid concentration. Cell viability was calculated through dividing with the absorbance of MTT formazan of AHA/BHA non-containing vehicle group. No significant differences were found in four groups. The concentration of tranexamic acid in culture medium was determined by HPLCMS/MS; 11.0 ± 2.5μg/mL in tranexamic acid in AHA/BHA non-containing formulation, 24.6 ± 3.8μg/mL in tranexamic acid in AHA/BHA containing formulation and not detected in vehicle groups. These findings demonstrated that reconstructed human epidermis can be used for the simultaneous evaluation of the skin permeability and irritancy of transdermal drug and cosmetic ingredients or products.
제5차 대한간학회 춘계학술대회 초록집 : B형 만성 간질환에서 HBV X, core promoter, precore변이종의 임상적 의의
이연재 ( Lee Yeon Jae ),양영일 ( Yang Yeong Il ),장원희 ( Jang Won Hui ),김석주 ( Kim Seog Ju ),장윤식 ( Jang Yun Sig ),이상혁 ( Lee Sang Hyeog ),설상영 ( Seol Sang Yeong ),정정명 ( Jeong Jeong Myeong ) 대한간학회 1999 Clinical and Molecular Hepatology(대한간학회지) Vol.5 No.1(S)
Sophora flavescens의 뿌리에서 유래한 Cholinesterase 저해 화합물
장원희, 트란홍광, 김장훈, 양서영, 김영호 충남대학교 약학대학 의약품개발연구소 2021 藥學論文集 Vol.36 No.-
Searching for cholinesterase inhibitory compounds from natural products is important to de- velop therapeutic agents for Alzheimer’s disease. Our previous research on the chemical components of Sophora flavescens roots resulted in the isolation of twelve flavonoids, kushenol E (1), maackiain (2), 8-prenylkaemperol (3), 8-prenylnaringenin (4), kushenol L (5), kushenol A (6), kushenol C (7), kushenol B (8), sophoraflavanone G (9), kushenol H (10), isoxanthohumol (11) and kurarinone (12) and their structures were elucidated by spectroscopic methods. Inhibitory effect of cholinesterase were tested using the Ellman's method. Most flavonoid compounds showed good inhibitory activities on cholinesterases. Kushenol E (1) and sophoraflavanone G (9) showed good inhibitory activities on acetylcholinesterase with IC50 values of 3.75 and 4.14 μM and butyrylcholinesterase with IC50 values of 2.00 and 0.75 μM, respectively. In addition, a kinetic analysis revealed that kushenol E (1) was non-competitive inhibitor with Ki value of 0.77 μM on acetylcholinesterase and 0.16 μM on butyrylcholinesterase, whereas sophoraflavanone G (9) was mixed type inhibitior with Ki value of 9.42 μM on acetylcholinesterase and 0.81 μM on butyrylcholinesterase. These findings suggest that flavonoid compounds from S. flavescens roots are potential cholinesterase inhibitors.